journal
https://read.qxmd.com/read/37737639/suggested-role-of-nosz-in-preventing-n-2-o-inhibition-of-dissimilatory-nitrite-reduction-to-ammonium
#1
JOURNAL ARTICLE
Sojung Yoon, Hokwan Heo, Heejoo Han, Dong-Uk Song, Lars R Bakken, Åsa Frostegård, Sukhwan Yoon
Climate change and nutrient pollution are among the most urgent environmental issues. Enhancing the abundance and/or the activity of beneficial organisms is an attractive strategy to counteract these problems. Dissimilatory nitrate reduction to ammonium (DNRA), which theoretically improves nitrogen retention in soils, has been suggested as a microbial process that may be harnessed, especially since many DNRA-catalyzing organisms have been found to possess nosZ genes and the ability to respire N2 O. However, the selective advantage that may favor these nosZ -harboring DNRA-catalyzing organisms is not well understood...
September 22, 2023: MBio
https://read.qxmd.com/read/37737636/assembling-the-anaerobic-gamma-butyrobetaine-to-tma-metabolic-pathway-in-escherichia-fergusonii-and-confirming-its-role-in-tma-production-from-dietary-l-carnitine-in-murine-models
#2
JOURNAL ARTICLE
Mohammed Dwidar, Jennifer A Buffa, Zeneng Wang, Akeem Santos, Aaron N Tittle, Xiaoming Fu, Adeline M Hajjar, Joseph A DiDonato, Stanley L Hazen
Trimethylamine-N-oxide (TMAO) is a major pro-atherogenic and pro-thrombotic metaorganismal molecule produced through the initial conversion of the dietary L -carnitine and other precursors into trimethylamine (TMA). We recently identified a dual-microbe anaerobic pathway for the metabolism of L- carnitine into TMA, in which the widely distributed cai operon in Enterobacteriaceae converts L- carnitine into gamma-butyrobetaine (γBB), followed by the degradation of γBB into TMA by the relatively rare <u>g</u>amma-<u>b</u>utyrobetaine <u>u</u>tilization ( gbu ) gene cluster present in Emergencia timonensis and few other related microbes...
September 22, 2023: MBio
https://read.qxmd.com/read/37737633/the-candida-albicans-reference-strain-sc5314-contains-a-rare-dominant-allele-of-the-transcription-factor-rob1-that-modulates-filamentation-biofilm-formation-and-oral-commensalism
#3
JOURNAL ARTICLE
Virginia E Glazier, Juraj Kramara, Tomye Ollinger, Norma V Solis, Robert Zarnowski, Rohan S Wakade, Min-Ju Kim, Gabriel J Weigel, Shen-Huan Liang, Richard J Bennett, Melanie Wellington, David R Andes, Mark A Stamnes, Scott G Filler, Damian J Krysan
Candida albicans is a diploid human fungal pathogen that displays significant genomic and phenotypic heterogeneity over a range of virulence traits and in the context of a variety of environmental niches. Here, we show that the effect of Rob1 on biofilm and filamentation virulence traits is dependent on both the specific environmental condition and the clinical strain of C. albicans . The C. albicans reference strain SC5314 is a ROB1 heterozygote with two alleles that differ by a single nucleotide polymorphism at position 946, resulting in a serine- or proline-containing isoform...
September 22, 2023: MBio
https://read.qxmd.com/read/37737632/conformational-variability-of-cyanobacterial-chli-the-aaa-motor-of-magnesium-chelatase-involved-in-chlorophyll-biosynthesis
#4
JOURNAL ARTICLE
Dmitry Shvarev, Alischa Ira Scholz, Arne Moeller
Magnesium chelatase is a conserved enzyme complex responsible for the first committed step of chlorophyll biosynthesis in photosynthetic organisms, which is the addition of magnesium to the chlorophyll precursor, protoporphyrin IX. The complex is composed of the catalytic subunit ChlH, the bridging subunit ChlD, and the subunit ChlI, which serves as the motor that drives the entire complex. Although the enzyme is well-characterized functionally, high-resolution structures are available only for individual subunits...
September 22, 2023: MBio
https://read.qxmd.com/read/37737622/enhanced-fungal-specificity-and-in-vivo-therapeutic-efficacy-of-a-c-22-modified-fk520-analog-against-c-neoformans
#5
JOURNAL ARTICLE
Angela Rivera, Won Young Lim, Eunchong Park, Patrick A Dome, Michael J Hoy, Ivan Spasojevic, Sheng Sun, Anna Floyd Averette, Sergio Pina-Oviedo, Praveen R Juvvadi, William J Steinbach, Maria Ciofani, Jiyong Hong, Joseph Heitman
Fungal infections are of mounting global concern, and the current limited treatment arsenal poses challenges when treating such infections. In particular, infections by Cryptococcus neoformans are associated with high mortality, emphasizing the need for novel therapeutic options. Calcineurin is a protein phosphatase that mediates fungal stress responses, and calcineurin inhibition by the natural product FK506 blocks C. neoformans growth at 37°C. Calcineurin is also required for pathogenesis. However, because calcineurin is conserved in humans and inhibition with FK506 results in immunosuppression, the use of FK506 as an anti-infective agent is precluded...
September 22, 2023: MBio
https://read.qxmd.com/read/37737612/human-gbp1-facilitates-the-rupture-of-the-legionella-containing-vacuole-and-inflammasome-activation
#6
JOURNAL ARTICLE
Antonia R Bass, Marisa S Egan, Jasmine Alexander-Floyd, Natasha Lopes Fischer, Jessica Doerner, Sunny Shin
The inflammasome is essential for host defense against intracellular bacterial pathogens, including Legionella pneumophila , the causative agent of the severe pneumonia Legionnaires' disease. Inflammasomes recruit and activate caspases, which promote IL-1 family cytokine release and pyroptosis to restrict infection. In mice, interferon (IFN) signaling promotes inflammasome responses against L. pneumophila and other bacteria , in part, through inducing a family of IFN-inducible GTPases known as guanylate-binding proteins (GBPs)...
September 22, 2023: MBio
https://read.qxmd.com/read/37737610/ultrastructure-and-3d-reconstruction-of-a-diplonemid-protist-diplonemea-and-its-novel-membranous-organelle
#7
JOURNAL ARTICLE
Daria Tashyreva, Jiří Týč, Aleš Horák, Julius Lukeš
Although diplonemid protists (Diplonemea, Euglenozoa) are among the most species-rich microeukaryotes in the ocean, many ultrastructural features of this lineage remain to be clarified. Using serial block-face scanning and transmission electron microscopy, we provide the whole-cell three-dimensional model of a previously undescribed diplonemid Lacrimia vacuolata sp. nov. For the first time, we were able to reconstruct a diplonemid cell in detail, including all its organelles and the flagellar and feeding apparatuses...
September 22, 2023: MBio
https://read.qxmd.com/read/37737607/crystal-structure-and-solution-state-of-the-c-terminal-head-region-of-the-narmovirus-receptor-binding-protein
#8
JOURNAL ARTICLE
Alice J Stelfox, Kasopefoluwa Y Oguntuyo, Ilona Rissanen, Karl Harlos, Robert Rambo, Benhur Lee, Thomas A Bowden
Increased viral surveillance has led to the isolation and identification of numerous uncharacterized paramyxoviruses, rapidly expanding our understanding of paramyxoviral diversity beyond the bounds of known genera. Despite this diversity, a key feature that unites paramyxoviruses is the presence of a receptor-binding protein (RBP), which facilitates host-cell attachment and plays a fundamental role in determining host range. Here, we study the RBP presented on the surface of rodent-borne paramyxoviruses Mossman and Nariva (MosV and NarV, respectively), viruses that constitute founding members of the recently defined Narmovirus genus within the Paramyxoviridae family...
September 22, 2023: MBio
https://read.qxmd.com/read/37737591/host-subversion-of-bacterial-metallophore-usage-drives-copper-intoxication
#9
JOURNAL ARTICLE
Saika Hossain, Jacqueline R Morey, Stephanie L Neville, Katherine Ganio, Jana N Radin, Javiera Norambuena, Jeff M Boyd, Christopher A McDevitt, Thomas E Kehl-Fie
Microorganisms can acquire metal ions in metal-limited environments using small molecules called metallophores. While metals and their importers are essential, metals can also be toxic, and metallophores have limited ability to discriminate between metals. The impact of metallophore-mediated non-cognate metal uptake on bacterial metal homeostasis and pathogenesis remains to be defined. The globally significant pathogen Staphylococcus aureus uses the Cnt system to secrete the metallophore staphylopine in zinc-limited host niches...
September 22, 2023: MBio
https://read.qxmd.com/read/37732809/pkr-mediated-stress-response-enhances-dengue-and-zika-virus-replication
#10
JOURNAL ARTICLE
Taissa Ricciardi-Jorge, Edroaldo Lummertz da Rocha, Edgar Gonzalez-Kozlova, Gabriela Flavia Rodrigues-Luiz, Brian J Ferguson, Trevor Sweeney, Nerea Irigoyen, Daniel Santos Mansur
The mechanisms by which flaviviruses use non-canonical translation to support their replication in host cells are largely unknown. Here, we investigated how the integrated stress response (ISR), which promotes translational arrest by eIF2ɑ phosphorylation (p-eIF2ɑ), regulates flavivirus replication. During dengue virus (DENV) and Zika virus (ZIKV) infection, eIF2ɑ phosphorylation peaked at 24 hours post-infection and was dependent on protein kinase RNA-activated (PKR) but not type I interferon...
September 21, 2023: MBio
https://read.qxmd.com/read/37732773/-glmpc-activated-by-gcn4-regulates-secondary-metabolism-under-nitrogen-limitation-conditions-in-ganoderma-lucidum
#11
JOURNAL ARTICLE
Zi Wang, Juhong Chen, Juan Ding, Jing Han, Liang Shi
Fungi actively alter their metabolic and synthetic pathways to cope with changing environmental conditions. The mitochondrial pyruvate carrier (MPC) plays a vital role in transporting pyruvate into mitochondria and regulating carbon metabolic homeostasis. However, the mechanism by which MPC regulates carbon metabolism in response to environmental stress in fungi remains unclear. In this study, yeast one-hybrid library screening assays were employed to screen for transcription factors in Ganoderma lucidum that may interact with GlMPC...
September 21, 2023: MBio
https://read.qxmd.com/read/37732764/f-actin-and-myosin-f-control-apicoplast-elongation-dynamics-which-drive-apicoplast-centrosome-association-in-toxoplasma-gondii
#12
JOURNAL ARTICLE
Parvathi Madhavi Devarakonda, Valeria Sarmiento, Aoife T Heaslip
Toxoplasma gondii contains an essential plastid organelle called the apicoplast that is necessary for fatty acid, isoprenoid, and heme synthesis. Perturbations affecting apicoplast function or inheritance lead to parasite death. The apicoplast is a single copy organelle and, therefore, must be divided so that each daughter parasite inherits an apicoplast during cell division. In this study, we identify new roles for F-actin and an unconventional myosin motor, TgMyoF, in this process. First, loss of TgMyoF and actin lead to an accumulation of apicoplast vesicles in the cytosol indicating a role for this actomyosin system in apicoplast protein trafficking or morphological integrity of the organelle...
September 21, 2023: MBio
https://read.qxmd.com/read/37732761/improved-scientific-knowledge-of-methanogenesis-and-methanotrophy-needed-to-slow-climate-change-during-the-next-30-years
#13
EDITORIAL
Eric A Davidson, Jeremy D Semrau, Nguyen K Nguyen
Owing to the high radiative forcing and short atmospheric residence time of methane, abatement of methane emissions offers a crucial opportunity for effective, rapid slowing of climate change. Here, we report on a colloquium jointly sponsored by the American Society for Microbiology and the American Geophysical Union, where 35 national and international experts from academia, the private sector, and government met to review understanding of the microbial processes of methanogenesis and methanotrophy. The colloquium addressed how advanced knowledge of the microbiology of methane production and consumption could inform waste management, including landfills and composts, and three areas of agricultural management: enteric emissions from ruminant livestock, manure management, and rice cultivation...
September 21, 2023: MBio
https://read.qxmd.com/read/37732760/recursive-genome-engineering-decodes-the-evolutionary-origin-of-an-essential-thymidylate-kinase-activity-in-pseudomonas-putida-kt2440
#14
JOURNAL ARTICLE
Nicolas T Wirth, Katja Rohr, Antoine Danchin, Pablo I Nikel
Thymidylate kinases (TMPKs) play an essential role in DNA biosynthesis across all domains of life by catalyzing dTMP phosphorylation to dTDP. In Pseudomonas putida KT2440, a model Gram-negative soil bacterium, tmk is disrupted by a 65-kb genomic island (GI), posing questions about the origin of the essential TMPK function. To solve this long-standing evolutionary riddle, we addressed three competing hypotheses: (i) assembly of two Tmk segments into a functional protein, (ii) complementation by a deoxynucleotide monophosphate kinase encoded within the GI, or (iii) fulfillment of the essential function by the product of PP_3363 , yet another gene annotated as "thymidylate kinase...
September 21, 2023: MBio
https://read.qxmd.com/read/37732738/surface-growth-of-pseudomonas-aeruginosa-reveals-a-regulatory-effect-of-3-oxo-c-12-homoserine-lactone-in-the-absence-of-its-cognate-receptor-lasr
#15
JOURNAL ARTICLE
Thays de Oliveira Pereira, Marie-Christine Groleau, Eric Déziel
Successful colonization of a multitude of ecological niches by the bacterium Pseudomonas aeruginosa relies on its ability to respond to concentrations of self-produced signal molecules. This intercellular communication system known as quorum sensing (QS) tightly regulates the expression of virulence determinants and a diversity of survival functions, including those required for social behaviors. In planktonic cultures of P. aeruginosa , the transcriptional regulator LasR is generally considered on top of the QS circuitry hierarchy; its activation relies on binding to 3-oxo-C12 -homoserine lactone (3-oxo-C12 -HSL), a product of LasI synthase...
September 21, 2023: MBio
https://read.qxmd.com/read/37729584/gene-duplication-gene-loss-and-recombination-events-with-variola-virus-shaped-the-complex-evolutionary-path-of-historical-american-horsepox-based-smallpox-vaccines
#16
JOURNAL ARTICLE
Aline R V Souza, Annika Brinkmann, José Esparza, Andreas Nitsche, Clarissa R Damaso
Vaccinia virus is the active component of all modern smallpox vaccines after the mid-20th century, but it is uncertain to what extent cowpox, vaccinia, and horsepox viruses were used to produce vaccines before then. Genome sequences of six smallpox vaccines used in the United States between 1850 and 1902, namely VK01, VK02, VK05, VK08, VK12, and Mulford_1902 vaccines, revealed >99.5% similarity with a 1976 strain of horsepox in the genome core. However, how these historical vaccines relate to horsepox and vaccinia viruses is still unknown...
September 20, 2023: MBio
https://read.qxmd.com/read/37728606/influence-of-soil-depth-irrigation-and-plant-genotype-on-the-soil-microbiome-metaphenome-and-carbon-chemistry
#17
JOURNAL ARTICLE
Katherine I Naasko, Daniel Naylor, Emily B Graham, Sneha P Couvillion, Robert Danczak, Nikola Tolic, Carrie Nicora, Steven Fransen, Haiying Tao, Kirsten S Hofmockel, Janet K Jansson
Climate change is causing an increase in drought in many soil ecosystems and a loss of soil organic carbon. Calcareous soils may partially mitigate these losses via carbon capture and storage. Here, we aimed to determine how irrigation-supplied soil moisture and perennial plants impact biotic and abiotic soil properties that underpin deep soil carbon chemistry in an unfertilized calcareous soil. Soil was sampled up to 1 m in depth from irrigated and planted field treatments and was analyzed using a suite of omics and chemical analyses...
September 20, 2023: MBio
https://read.qxmd.com/read/37728370/the-morphogenic-protein-copd-controls-the-spatio-temporal-dynamics-of-pbp1a-and-pbp2b-in-streptococcus-pneumoniae
#18
JOURNAL ARTICLE
Cassandra Lenoir, Anaïs Pelletier, Sylvie Manuse, Hugo Millat, Adrien Ducret, Anne Galinier, Thierry Doan, Christophe Grangeasse
Penicillin-binding proteins (PBPs) are key to the assembly of peptidoglycan, the major component of the bacterial cell wall. Although several PBP-specific regulatory proteins have been identified in different species, little is known about how the activity of PBPs is controlled and coordinated during the cell cycle. In this study, we characterize the unknown function protein Spr1400 and demonstrate its regulatory function on two PBPs in Streptococcus pneumoniae . For that, we use a combination of technics ranging from bacterial genetics and protein biochemistry to microscopy imaging...
September 20, 2023: MBio
https://read.qxmd.com/read/37728345/virb-a-key-transcriptional-regulator-of-shigella-virulence-requires-a-ctp-ligand-for-its-regulatory-activities
#19
JOURNAL ARTICLE
Taylor M Gerson, Audrey M Ott, Monika M A Karney, Jillian N Socea, Daren R Ginete, Lakshminarayan M Iyer, L Aravind, Ronald K Gary, Helen J Wing
The VirB protein, encoded by the large virulence plasmid of Shigella spp., is a key transcriptional regulator of virulence genes. Without virB , Shigella cells are avirulent. On the virulence plasmid, VirB functions to offset transcriptional silencing mediated by the nucleoid structuring protein, H-NS, which binds and sequesters AT-rich DNA, making it inaccessible for gene expression. Thus, gaining a mechanistic understanding of how VirB counters H-NS-mediated silencing is of considerable interest. VirB is unusual in that it does not resemble classic transcription factors...
September 20, 2023: MBio
https://read.qxmd.com/read/37728338/-l-pneumophila-resists-its-self-harming-metabolite-hga-via-secreted-factors-and-collective-peroxide-scavenging
#20
JOURNAL ARTICLE
Mische Holland, Danielle N Farinella, Emily Cruz-Lorenzo, Madelyn I Laubscher, Darian A Doakes, Maria A Ramos, Nanami Kubota, Tera C Levin
Many pathogenic bacteria, including Legionella pneumophila , infect humans from environmental reservoirs. To survive in these reservoirs, bacteria must withstand microbe-on-microbe competition. We previously discovered that L. pneumophila can compete with neighboring bacteria via an antimicrobial metabolite called homogentisic acid (HGA). Curiously, L. pneumophila strains that secrete HGA are not wholly immune to its effects: low-density bacteria are strongly inhibited by HGA, whereas high-density cells are tolerant...
September 20, 2023: MBio
journal
journal
42716
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.