Surachai Ngamratanapaiboon, Krittaboon Pornchokchai, Siriphattarinya Wongpitoonmanachai, Petchlada Pholkla, Napatarin Srikornvit, Jiajun Mo, Patipol Hongthawonsiri, Pracha Yambangyang, Pilaslak Akrachalanont
BACKGROUND AND PURPOSE: The use of fluoxetine raises the risk of pancreatic beta-cell dysfunction. However, the specific mechanism behind its mechanism of action in beta cells is unknown. This study investigated the cellular response of MIN6 cells to fluoxetine using untargeted cell-based metabolomics. EXPERIMENTAL APPROACH: Metabolic profiling of MIN6 cells was performed using liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis on samples prepared under optimized conditions, followed by principal component analysis, partial least squares-discriminant analysis, and pair-wise orthogonal projections to latent structures discriminant analyses...
2023: Research in Pharmaceutical Sciences