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International Journal of Microbiology

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https://read.qxmd.com/read/31093290/encapsulation-of-bifidobacterium-pseudocatenulatum-strain-g4-within-bovine-gelatin-genipin-sodium-alginate-combinations-optimisation-approach-using-face-central-composition-design-response-surface-methodology-fccd-rsm
#1
Khalilah Abdul Khalil, Suhaimi Mustafa, Rosfarizan Mohammad, Arbakariya Bin Ariff, Siti Aqlima Ahmad, Farrah Aini Dahalan, Mohd Yazid Abdul Manap
Bovine gelatin is a biopolymer which has good potential to be used in encapsulating matrices for probiotic candidate Bifidobacterium pseudocatenulatum strain G4 (G4) because of its amphoteric nature characteristic. Beads were prepared by the extrusion method using genipin and sodium alginate as a cross-linking agent. The optimisation of bovine gelatin-genipin-sodium alginate combinations was carried out using face central composition design (FCCD) to investigate G4 beads' strength, before and after exposed to simulated gastric (SGF), intestinal fluids (SIF), and encapsulation yield...
2019: International Journal of Microbiology
https://read.qxmd.com/read/31089326/bacteriological-assessment-of-the-indoor-air-of-different-hospitals-of-kathmandu-district
#2
Arzu Kunwar, Samyukta Tamrakar, Shyaron Poudel, Sony Sharma, Pramila Parajuli
Nosocomial infection is the infection that has been caught in a hospital and is potentially caused by organisms that are not susceptible to antibiotics. Nosocomial infections are transmitted directly or indirectly through air and may cause different types of infections. This study was undertaken with an objective to determine the prevalence of nosocomial bacteria present in hospital indoor environment. A total of 16 air samples were taken from general wards and emergency wards of 8 different hospitals using an impactor air sampler in nutrient agar, mannitol salt agar, blood agar, cetrimide agar, and MacConkey agar...
2019: International Journal of Microbiology
https://read.qxmd.com/read/31073310/antibacterial-activity-of-honey-against-methicillin-resistant-staphylococcus-aureus-a-laboratory-based-experimental-study
#3
Mohammedaman Mama, Teklu Teshome, Jafer Detamo
Background: Antimicrobial drug resistance is one of the serious issues this world is facing nowadays, and increased cost of searching for effective antimicrobial agents and the decreased rate of new drug discovery have made the situation increasingly worrisome. Objective: The aim of this study is to determine in vitro antibacterial activity of honey against methicillin-resistant Staphylococcus aureus isolates from wound infection. Methods: An experimental study was conducted from May to November 2017...
2019: International Journal of Microbiology
https://read.qxmd.com/read/31065270/methicillin-and-inducible-clindamycin-resistant-staphylococcus-aureus-among-patients-with-wound-infection-attending-arba-minch-hospital-south-ethiopia
#4
Mohammedaman Mama, Addis Aklilu, Kassahun Misgna, Molla Tadesse, Eyerusalem Alemayehu
Background: Wound infection is one of the most common hospital-acquired infections. Different bacteria cause infection, of which Staphylococcus aureus is one of the known bacteria in causing infection with increased drug-resistant isolates. Objective: To assess the prevalence and antimicrobial susceptibility pattern of methicillin and inducible clindamycin-resistant Staphylococcus aureus among patients with wound infections attending Arba Minch Hospital. Methods: A facility-based cross-sectional study was conducted from April to June 2017...
2019: International Journal of Microbiology
https://read.qxmd.com/read/31001341/comparison-of-serum-potassium-magnesium-and-calcium-levels-between-kanamycin-and-capreomycin-based-regimen-treated-multidrug-resistant-tuberculosis-patients-in-bandung-cease-mdr-tb-a-retrospective-cohort-study
#5
Arto Yuwono Soeroto, Guntur Darmawan, Rudi Supriyadi, Panji Gugah Bhaskara, Prayudi Santoso, Bachti Alisjahbana, Ida Parwati
Treatment of multidrug-resistant tuberculosis (MDR-TB) with second-line injectable drugs may result in an electrolyte imbalance. This retrospective study was performed to compare and evaluate the effect of kanamycin and capreomycin on serum potassium, calcium, and magnesium in the first and second month treatment at a tertiary, top-referral hospital in Bandung, Indonesia. Data from 84 subjects with complete medical records of at least serum potassium during either kanamycin-based or capreomycin-based treatment were retrieved from the institutional database...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30956662/characterization-and-synergistic-antimicrobial-evaluation-of-lipopeptides-from-bacillus-amyloliquefaciens-isolated-from-oil-contaminated-soil
#6
Pratiwi Sudarmono, Ahmad Wibisana, Lira W Listriyani, Saleha Sungkar
Lipopeptides show great potential for biomedical application. Several lipopeptides exhibit narrow and broad-spectrum inhibition activities. The aim of the study is to characterize the lipopeptides produced by B. amyloliquefaciens strain MD4-12 and evaluate the synergistic antimicrobial activity in combination with a conventional antibiotic against Gram-negative bacteria. B. amyloliquefaciens strain MD4-12 was isolated from oil-contaminated soil. The isolate was cultivated in McKeen medium, and the lipopeptides were isolated by precipitation and extraction with methanol...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30949206/microbiota-landscape-of-gut-system-of-guppy-fish-poecilia-reticulata-plays-an-outstanding-role-in-adaptation-mechanisms
#7
Christian Aimé Kayath, Armel Ibala Zamba, Joseph Goma-Tchimbakala, Victor Mamonékéné, Gloire Moniceth Mombo Makanga, Aimé Augustin Lebonguy, Etienne Nguimbi
Microbial consortium that is present in fish gut systems works together to achieve unknown specific roles. Here, we collected guppy fish from hydrocarbon- and trace metal-contaminated wastewater to assess the relationships between gut microbiota and host fish adaptation. Targeted genes and 16S rRNA amplicon sequencing have been used to identify gut bacteria of guppies. Mineral-conditioned medium contributes to identify bacteria with the ability to grow and/or to tolerate hydrocarbon and trace metals. Additionally, trace metals' tolerance minimum inhibitory concentration (MIC) of microbiota was evaluated...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30944569/smear-positive-tuberculosis-prevalence-and-associated-factors-among-pregnant-women-attending-antinatal-care-in-north-gondar-zone-hospitals-ethiopia
#8
Adugna Berju, Belete Haile, Seleshe Nigatu, Araya Mengistu, Girma Birhan
Tuberculosis is an ancient infectious disease that remains a threat to public health around the world. It is a contagious airborne disease caused by Mycobacterium tuberculosis complex. In high tuberculosis burden countries, the prevalence of tuberculosis was 10-fold higher in the HIV-infected mothers than that in those not infected with HIV. However, little is known about the burden of tuberculosis (TB) and associated factors in women of reproductive age in most resource poor countries. Therefore, this study aims to investigate prevalence of smear-positive TB and factors associated in pregnant women attending antenatal care in North West, Ethiopia...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30944568/relationship-of-sanitation-parameters-with-microbial-diversity-and-load-in-raw-meat-from-the-outlets-of-the-metropolitan-city-biratnagar-nepal
#9
Sanjay Mahato
The main aim of this study is to assess the microbial load of raw meat from outlets of Biratnagar and its relationship with several sanitation parameters. Samples were taken from meat outlets, and required microbiological procedures were followed as per guidelines. Approximately 63.6% of microbes were present in meat with poor sanitation while 36.4% were present in meat with good sanitation. Fungal contamination in poorly kept mutton was one and half times greater than chicken/mutton of good sanitation. Fungi such as Penicillium (21...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30906324/perchlorate-reducing-bacteria-from-hypersaline-soils-of-the-colombian-caribbean
#10
Rosa Acevedo-Barrios, Angela Bertel-Sevilla, Jose Alonso-Molina, Jesus Olivero-Verbel
Perchlorate (ClO4 - ) has several industrial applications and is frequently detected in environmental matrices at relevant concentrations to human health. Currently, perchlorate-degrading bacteria are promising strategies for bioremediation in polluted sites. The aim of this study was to isolate and characterize halophilic bacteria with the potential for perchlorate reduction. Ten bacterial strains were isolated from soils of Galerazamba-Bolivar, Manaure-Guajira, and Salamanca Island-Magdalena, Colombia. Isolates grew at concentrations up to 30% sodium chloride...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30906323/prevalence-of-bacterial-urinary-tract-infections-and-associated-factors-among-patients-attending-hospitals-in-bushenyi-district-uganda
#11
Martin Odoki, Adamu Almustapha Aliero, Julius Tibyangye, Josephat Nyabayo Maniga, Eddie Wampande, Charles Drago Kato, Ezera Agwu, Joel Bazira
Urinary tract infections (UTIs) are one of the major causes of morbidity and comorbidities in patients with underlying conditions, and it accounts for the majority of the reasons for hospital visit globally. Sound knowledge of factors associated with UTI may allow timely intervention that can easily bring the disease under control. This study was designed to determine the prevalence of UTI by isolating and characterizing the different bacterial etiological agents and to evaluate the factors associated with UTI...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30881456/magnitude-of-biofilm-formation-and-antimicrobial-resistance-pattern-of-bacteria-isolated-from-urinary-catheterized-inpatients-of-jimma-university-medical-center-southwest-ethiopia
#12
Netsanet Awoke, Tesfaye Kassa, Lule Teshager
Biofilm formation is one of the features of most bacteria. Catheterization in medicine is a source of highly resistant bacterial infections, and those bacteria respond poorly to antimicrobial therapy. Bacterial biofilm features were not described from catheterized inpatients in Ethiopia as its formation is known to afford antimicrobial resistance and challenge patient management. The aim of this study was to isolate catheter-associated urinary bacterial pathogens, their biofilm formation, and antimicrobial susceptibility pattern among inpatients of Jimma University Medical Center (JUMC) in Southwest Ethiopia...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30805002/screening-and-antifungal-activity-of-a-%C3%AE-carboline-derivative-against-cryptococcus-neoformans-and-c-gattii
#13
Kátia Santana Cruz, Emerson Silva Lima, Marcia de Jesus Amazonas da Silva, Erica Simplício de Souza, Andreia Montoia, Adrian Martin Pohlit, João Vicente Braga de Souza
Background: Cryptococcosis is a fungal disease of bad prognosis due to its pathogenicity and the toxicity of the drugs used for its treatment. The aim of this study was to investigate the medicinal potential of carbazole and β -carboline alkaloids and derivatives against Cryptococcus neoformans and C. gattii . Methods: MICs were established in accordance with the recommendations of the Clinical and Laboratory Standards Institute for alkaloids and derivatives against C...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30766603/isolation-of-polygalacturonase-producing-bacterial-strain-from-tomatoes-lycopersicon-esculentum-mill
#14
Yemisi Dorcas Obafemi, Adesola Adetutu Ajayi, Olugbenga Samson Taiwo, Shade John Olorunsola, Patrick Omoregie Isibor
Background: Polygalacturonase (EC 3.2.1.15) enzyme aids in microbial spoilage of fruits and vegetables. It is very important to find economical ways to producing the enzyme so as to achieve maximum yield in industries due to its use at different areas of production process. Methods: Isolation of polygalacturonase-producing bacterial strain from tomatoes ( Lycopersicon esculentum Mill.) was studied. Polygalacturonase-producing bacterial strains were isolated and screened from tomatoes stored at normal laboratory temperature (25 ± 2°C)...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30766602/melittin-inhibition-and-eradication-activity-for-resistant-polymicrobial-biofilm-isolated-from-a-dairy-industry-after-disinfection
#15
Emilia Galdiero, Antonietta Siciliano, Renato Gesuele, Valeria Di Onofrio, Annarita Falanga, Angela Maione, Renato Liguori, Giovanni Libralato, Marco Guida
The emerging concern about the increase of antibiotic resistance and associated biofilm has encouraged scientists to look for alternative antibiotics such as antimicrobial peptides (AMPs). This study evaluated the ability of melittin to act as an antibacterial biofilm inhibitor and biofilm remover considering isolates from dairy industry. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), minimum biofilm inhibitory concentrations (MBICs), and biofilm removal activities were studied in polymicrobial biofilms produced from isolates...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30719044/antibacterial-potential-of-aloe-weloensis-aloeacea-leaf-latex-against-gram-positive-and-gram-negative-bacteria-strains
#16
Yohannes Kelifa Emiru, Ebrahim Abdela Siraj, Tekleab Teka Teklehaimanot, Gedefaw Getnet Amare
Objective: To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods: The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623)...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30723505/arsenic-resistance-and-biosorption-by-isolated-rhizobacteria-from-the-roots-of-ludwigia-octovalvis
#17
Harmin Sulistiyaning Titah, Siti Rozaimah Sheikh Abdullah, Mushrifah Idris, Nurina Anuar, Hassan Basri, Muhammad Mukhlisin, Bieby Voijant Tangahu, Ipung Fitri Purwanti, Setyo Budi Kurniawan
Certain rhizobacteria can be applied to remove arsenic in the environment through bioremediation or phytoremediation. This study determines the minimum inhibitory concentration (MIC) of arsenic on identified rhizobacteria that were isolated from the roots of Ludwigia octovalvis (Jacq.) Raven. The arsenic biosorption capability of the was also analyzed. Among the 10 isolated rhizobacteria, five were Gram-positive ( Arthrobacter globiformis , Bacillus megaterium, Bacillus cereus, Bacillus pumilus , and Staphylococcus lentus ), and five were Gram-negative ( Enterobacter asburiae, Sphingomonas paucimobilis, Pantoea spp...
2018: International Journal of Microbiology
https://read.qxmd.com/read/30693035/magnitude-of-vancomycin-resistant-enterococci-vre-colonization-among-hiv-infected-patients-attending-art-clinic-in-west-amhara-government-hospitals
#18
Manamenot Agegne, Bayeh Abera, Awoke Derbie, Gizachew Yismaw, Melashu Balew Shiferaw
Background: Enterococci that colonize the intestinal tract of immunocompromised patients are an important cause of nosocomial infections. Data on the prevalence of vancomycin-resistant Enterococci (VRE) and its antimicrobial susceptibility patterns and associated factors are scarce in the present study area. Therefore, this study was conducted aimed at determining the prevalence of VRE colonization among HIV-infected patients attending ART clinic at West Amhara Government Hospitals. Methods: A cross-sectional study was conducted from 1 February 2017 to 31 May 2017...
2018: International Journal of Microbiology
https://read.qxmd.com/read/30631365/molecular-characterization-of-low-density-polyethene-ldpe-degrading-bacteria-and-fungi-from-dandora-dumpsite-nairobi-kenya
#19
Christabel Ndahebwa Muhonja, Gabriel Magoma, Mabel Imbuga, Huxley Mae Makonde
This study aimed at molecular and biochemical characterization of low-density polyethene (LDPE) degrading fungi and bacteria from Dandora dumpsite, Nairobi. Twenty bacterial and 10 fungal isolates were identified using 16S rDNA and 18S rDNA sequences for bacteria and fungi, respectively. The highest fungal degradation was attributed to Aspergillus oryzae strain A5,1 while the highest bacterial degradation was attributed to Bacillus cereus strain A5,a and Brevibacillus borstelensis strain B2,2, respectively...
2018: International Journal of Microbiology
https://read.qxmd.com/read/30631364/-enterococcus-faecium-tir-domain-genes-are-part-of-a-gene-cluster-which-promotes-bacterial-survival-in-blood
#20
Theresa M Wagner, Jessin Janice, Fernanda L Paganelli, Rob J Willems, Fatemeh Askarian, Torunn Pedersen, Janetta Top, Carla de Haas, Jos A van Strijp, Mona Johannessen, Kristin Hegstad
Enterococcus faecium has undergone a transition to a multidrug-resistant nosocomial pathogen. The population structure of E. faecium is characterized by a sharp distinction of clades, where the hospital-adapted lineage is primarily responsible for bacteremia. So far, factors that were identified in hospital-adapted strains and that promoted pathogenesis of nosocomial E. faecium mainly play a role in adherence and biofilm production, while less is known about factors contributing to survival in blood. This study identified a gene cluster, which includes genes encoding bacterial Toll/interleukin-1 receptor- (TIR-) domain-containing proteins (TirEs)...
2018: International Journal of Microbiology
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