Camilo Castellar-Mendoza, María-Angélica Calderón-Peláez, Jaime E Castellanos, Myriam L Velandia-Romero, Carolina Coronel-Ruiz, Sigrid Camacho-Ortega, Lilia J Bernal-Cepeda, Lady López-Ibarra, Jhann A Arturo, Félix G Delgado, Hernando Gutierrez-Barbosa, Sonia Bohorquez-Avila, Johanna Madroñero, Zayda L Corredor-Rozo, Sandra J Perdomo-Lara, Angela Fonseca-Benitez, Eliana Calvo
PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives. In-house multiplex qPCR tests were developed worldwide to detect different viral targets and ensure results, follow the infections, and favor the containment of a pandemic...
2024: International Journal of Microbiology