Read by QxMD icon Read


Shanwen Ke, Shuchun Liu, Xin Luan, Xin-Ming Xie, Tzung-Fu Hsieh, Xiang-Qian Zhang
Leaf senescence is a genetically regulated, highly complex and ordered process. Although it has been extensively studied, the mechanism of leaf senescence is not well understood. In this study, we isolated a rice mutant, designated as premature senescence leaf (psl), which exhibits early senescence and spontaneous lesion mimic phenotype after flowering. The psl mutant displays programmed cell death with elevated accumulation of reactive oxygen species (ROS). Molecular and genetic analyses revealed that the phenotypes were caused by a phenylalanine deletion in the OsPSL (LOC_Os12g42420) that encode a putative core 2/I branching beta-1,6-N-acetylglucosaminyl transferase predicted to be involved in protein glycosylation modification...
February 13, 2019: Rice
Jun Wang, Qiang Zhang, Yi Wang, Jing Huang, Nengjie Luo, Shengbo Wei, Jian Jin
BACKGROUND: TRIANGULAR HULL1 (TH1), a member of the rice ALOG gene family, has been characterized as a rice lemma/palea-related gene. To understand the gene regulatory network that controlled by TH1, we analyzed the transcriptome from a TH1 knock out (KO) line, which was generated by CRISPR/Cas9. Our study may shed some light on the molecular mechanism of lemma/palea development. RESULTS: We obtained 20 T0 th1-C transgenic plants by CRISPR/Cas9. Among the 20 plants, there were eight bi-allelic mutations, five homozygous mutations, three heterozygous mutations, and four Non-KO plants...
February 6, 2019: Rice
Xiangbo Li, Quandan Tao, Jun Miao, Zefeng Yang, Minghong Gu, Guohua Liang, Yong Zhou
BACKGROUND: qPE9-1/DEP1, encoding a G protein γ subunit, has multiple effects on plant architecture, grain size, and yield in rice. The qPE9-1 protein contains an N-terminal G gamma-like (GGL) domain, a putative transmembrane domain, and a C-terminal cysteine-rich domain. However, the roles of each domain remain unclear. RESULTS: In the present study, we focused on the genetic effects of different domains of qPE9-1 in the regulation of grain length and weight. We generated a series of transgenic plants expressing different truncated qPE9-1 proteins through constitutive expression and clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 strategies...
January 31, 2019: Rice
Nita Lakra, Charanpreet Kaur, Sneh Lata Singla-Pareek, Ashwani Pareek
BACKGROUND: To delineate the adaptive mechanisms operative under salinity stress, it is essential to study plant responses at the very early stages of stress which are very crucial for governing plant survival and adaptation. We believe that it is the initial perception and response phase which sets the foundation for stress adaptation in rice seedlings where plants can be considered to be in a state of osmotic shock and ion buildup. RESULTS: An isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach was used to analyze the pre-existing differences as well as the very early salt shock responsive changes in the proteome of seedlings of contrasting rice genotypes, viz salt-sensitive IR64 and salt-tolerant Pokkali...
January 30, 2019: Rice
Giang Thi Hoang, Lam Van Dinh, Thom Thi Nguyen, Nhung Kim Ta, Floran Gathignol, Chung Duc Mai, Stefan Jouannic, Khanh Dang Tran, Trung Huu Khuat, Vinh Nang Do, Michel Lebrun, Brigitte Courtois, Pascal Gantet
BACKGROUND: Drought tolerance is a major challenge in breeding rice for unfavorable environments. In this study, we used a panel of 180 Vietnamese rice landraces genotyped with 21,623 single-nucleotide polymorphism markers to perform a genome-wide association study (GWAS) for different drought response and recovery traits during the vegetative stage. These landraces originate from different geographical locations and are adapted to different agrosystems characterized by contrasted water regimes...
January 28, 2019: Rice
Xingxue Mao, Jianjun Zhang, Wuge Liu, Shijuan Yan, Qing Liu, Hua Fu, Junliang Zhao, Wenjie Huang, Jingfang Dong, Shaohong Zhang, Tifeng Yang, Wu Yang, Bin Liu, Feng Wang
BACKGROUND: Seed dormancy directly affects the phenotype of pre-harvest sprouting, and ultimately affects the quality and yield of rice seeds. Although many genes controlling seed dormancy have been cloned from cereals, the regulatory mechanisms controlling this process are complex, and much remains unknown. The MAPK cascade is involved in many signal transduction pathways. Recently, MKK3 has been reported to be involved in the regulation of seed dormancy, but its mechanism of action is unclear...
January 22, 2019: Rice
Funing Meng, Dan Xiang, Jianshu Zhu, Yong Li, Chuanzao Mao
Roots are fundamentally important for growth and development, anchoring the plant to its growth substrate, facilitating water and nutrient uptake from the soil, and sensing and responding to environmental signals such as biotic and abiotic stresses. Understanding the molecular mechanisms controlling root architecture is essential for improving nutrient uptake efficiency and crop yields. In this review, we describe the progress being made in the identification of genes and regulatory pathways involved in the development of root systems in rice (Oryza sativa L...
January 10, 2019: Rice
Miwa Ohashi, Keiki Ishiyama, Miyako Kusano, Atsushi Fukushima, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya
BACKGROUND: Our previous transcriptomic analysis revealed that downregulation of nitrogen and carbon metabolism in the basal portions of the shoots inhibited cytosolic glutamine synthetase1;2 (GS1;2), which severely reduced rice tiller number. In the present study, we used rice mutants lacking GS1;2 (gs1;2 mutants) to determine the contribution of carbon metabolism to tiller growth. RESULTS: Metabolomic analysis indicated the effects of carbon metabolism disorder such as reductions in the levels of sugar metabolites (e...
December 22, 2018: Rice
Yue Chen, Xiyang Wu, Chenggang Li, Yibo Zeng, Xinqiu Tan, Deyong Zhang, Yong Liu
BACKGROUND: GPI-anchoring is a prevalent Glycosylphosphatidylinositol modification process of posttranslational protein and is necessary for cell wall integrity in eukaryotes. To date, the function of GPI anchored-related protein remains unknown in phytopathogenic fungi. RESULTS: We here characterized the functions of MoPer1, a homolog of Saccharomyces cerevisiae ScPer1, from the rice blast fungus Magnaporthe oryzae. Transcriptional analysis demonstrated that MoPER1 was significantly upregulated during conidiation and infection...
December 22, 2018: Rice
Yoav Yichie, Chris Brien, Bettina Berger, Thomas H Roberts, Brian J Atwell
BACKGROUND: Soil salinity is widespread in rice-producing areas globally, restricting both vegetative growth and grain yield. Attempts to improve the salt tolerance of Asian rice, Oryza sativa-the most salt sensitive of the major cereal crops-have met with limited success, due to the complexity of the trait and finite variation in salt responses among O. sativa lines. Naturally occurring variation among the more than 20 wild species of the Oryza genus has great potential to provide breeders with novel genes to improve resistance to salt...
December 22, 2018: Rice
Lin Zhang, Yusuke Nakagomi, Takashi Endo, Mika Teranishi, Jun Hidema, Shusei Sato, Atsushi Higashitani
BACKGROUND: The rice blast resistance gene Pi54 was cloned from Oryza sativa ssp. indica cv. Tetep, which conferred broad-spectrum resistance against Magnaporthe oryzae. Pi54 allelic variants have been identified in not only domesticates but also wild rice species, but the majority of japonica and some indica cultivars lost the function. RESULTS: We here found that Pi54 (Os11g0639100) and its homolog Os11g0640600 (named as #11) were closely located on a 25 kbp region in japonica cv...
December 5, 2018: Rice
Mohamed Hazman, Kathleen M Brown
BACKGROUND: Root architectural and anatomical phenotypes are important for adaptation to drought. Many rice-growing regions face increasing water scarcity. This study describes drought responses of 11 Egyptian rice cultivars with emphasis on plastic root responses that may enhance drought adaptation. RESULTS: Eleven Egyptian rice cultivars were phenotyped for root architectural and anatomical traits after 6 weeks growth in soil mesocosms under well-watered conditions...
December 4, 2018: Rice
Junliang Zhao, Wu Yang, Shaohong Zhang, Tifeng Yang, Qin Liu, Jingfang Dong, Hua Fu, Xingxue Mao, Bin Liu
BACKGROUND: Cadmium (Cd) accumulation in rice followed by transfer to the food chain causes severe health problems in humans. Breeding of low Cd accumulation varieties is one of the most economical ways to solve the problem. However, information on the identity of rice germplasm with low Cd accumulation is limited, particularly in indica, and the genetic basis of Cd accumulation in rice is not well understood. RESULTS: Screening of 312 diverse rice accessions revealed that the grain Cd concentrations of these rice accessions ranged from 0...
November 21, 2018: Rice
Fan Zhang, Yu-Chan Zhang, Jin-Ping Zhang, Yang Yu, Yan-Fei Zhou, Yan-Zhao Feng, Yu-Wei Yang, Meng-Qi Lei, Huang He, Jian-Pin Lian, Yue-Qin Chen
BACKGROUND: Pollen tube formation and growth are crucial steps that lead to seed production. Despite the importance of pollen tube growth, the molecular mechanisms implicated in its spatial and temporal control are not fully known. In this study, we found an uclacyanin gene, OsUCL8, that regulates pollen intine deposition and pollen tube growth. FINDINGS: The overexpression of OsUCL8 led to a striking irregularity in pollen tube growth and pollination and thus affected the seed setting rate in rice; many pollen tubes appeared to lose the ability to grow directly into the style...
November 19, 2018: Rice
Jiayan Liang, Shaoying Guo, Bo Sun, Qing Liu, Xionghui Chen, Haifeng Peng, Zemin Zhang, Qingjun Xie
Leaf rolling is one of the most significant symptoms of drought stress in plant. Previously, we identified a dominant negative mutant, termed rolled and erect 1 (hereafter referred to rel1-D), regulating leaf rolling and erectness in rice. However, the role of REL1 in drought response is still poorly understood. Here, our results indicated that rel1-D displayed higher tolerance to drought relative to wild type, and the activity of superoxide dismutase (SOD) and drought responsive genes were significantly up-regulated in rel1-D...
October 25, 2018: Rice
Zhenyi Chang, Mingna Jin, Wei Yan, Hui Chen, Shijun Qiu, Shan Fu, Jixing Xia, Yuchen Liu, Zhufeng Chen, Jianxin Wu, Xiaoyan Tang
BACKGROUND: The pollen wall, which protects male gametophyte against various stresses and facilitates pollination, is essential for successful reproduction in flowering plants. The pollen wall consists of gametophyte-derived intine and sporophyte-derived exine. From outside to inside of exine are tectum, bacula, nexine I and nexine II layers. How these structural layers are formed has been under extensive studies, but the molecular mechanisms remain obscure. RESULTS: Here we identified two osabcg3 allelic mutants and demonstrated that OsABCG3 was required for pollen development in rice...
October 11, 2018: Rice
Laurent Sagart, Tze-Fu Hsu, Yuan-Ching Tsai, Cheng-Chieh Wu, Lin-Tzu Huang, Yu-Chi Chen, Yi-Fang Chen, Yu-Chien Tseng, Hung-Ying Lin, Yue-Ie Caroline Hsing
BACKGROUND: Genetic data for traditional Taiwanese (Formosan) agriculture is essential for tracing the origins on the East Asian mainland of the Austronesian language family, whose homeland is generally placed in Taiwan. Three main models for the origins of the Taiwanese Neolithic have been proposed: origins in coastal north China (Shandong); in coastal central China (Yangtze Valley), and in coastal south China. A combination of linguistic and agricultural evidence helps resolve this controversial issue...
October 11, 2018: Rice
Rosa Paula O Cuevas, Cyril John Domingo, Nese Sreenivasulu
BACKGROUND: For predicting texture suited for South and South East Asia, most of the breeding programs tend to focus on developing rice varieties with intermediate to high amylose content in indica subspecies. However, varieties within the high amylose content class may still be distinguishable by consumers, who are able to distinguish texture that cannot be differentiated by proxy cooking quality indicators. RESULTS: This study explored a suite of assays to capture viscosity, rheometric, and mechanical texture parameters for characterising cooked rice texture in a set of 211 rice accessions from a diversity panel and employed multivariate approaches to classify rice varieties into distinct cooking quality classes...
October 10, 2018: Rice
Jie Hu, Xingyuan Chang, Ling Zou, Weiqi Tang, Weiren Wu
BACKGROUND: Host-plant resistance is the most desirable and economic way to overcome BPH damage to rice. As single-gene resistance is easily lost due to the evolution of new BPH biotypes, it is urgent to explore and identify new BPH resistance genes. RESULTS: In this study, using F2:3 populations and near-isogenic lines (NILs) derived from crosses between two BPH-resistant Sri Lankan rice cultivars (KOLAYAL and POLIYAL) and a BPH-susceptible cultivar 9311, a new resistance gene Bph33 was fine mapped to a 60-kb region ranging 0...
October 5, 2018: Rice
Zhiming Yu, Lixiang Dong, Zhifang Jiang, Keke Yi, Jianhua Zhang, Zhongchen Zhang, Zhenxing Zhu, Yuhuan Wu, Maojun Xu, Jun Ni
The mechanisms of plant defense against pathogen attack in plant leaves have been extensively studied. However, our understanding of plant defense mechanisms in plant roots is still limited. In this study, a semi-dominant mutant nrtp1-D (necrotic root tip 1), with a short-root phenotype, was characterized in rice. Map-based cloning revealed that NRTP1 encoded a typical coiled-coil nucleotide binding leucine rich repeat (CC-NB-LRR) type protein and the mutation caused an amino acid substitution in the Nucleotide-Binding adaptor shared by Apaf1, certain R genes and CED4 (NB-ARC) domain, which may cause constitutive auto-activation of the NRTP1 protein...
October 3, 2018: Rice
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"