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Probiotics and Antimicrobial Proteins

Ourdia Kareb, Mohammed Aïder
It is well established that bacteria communicate between each other by using different mechanisms; among which, quorum sensing (QS) is the best known one. Indeed, intra- and intercellular communications of microorganisms, as well as the regulation of metabolism and reaction to the surrounding environmental conditions, are carried out by using different signaling molecules. N-Acyl homoserine lactones control the QS in Gram-negative bacteria, while Gram-positive bacteria use communicating peptides. These compounds, by diffusing through the bacterial membrane cell from the extracellular medium, directly or indirectly control the expression of specific genes that induce bacteria to react to their surrounding environment and stressing agents...
May 19, 2019: Probiotics and Antimicrobial Proteins
Mahmoud A O Dawood, Fawzy I Magouz, Mahmoud F I Salem, Zizy I Elbialy, Hanaa A Abdel-Daim
The current study was conducted to evaluate the synergetic effects of heat-killed Lactobacillus plantarum (HK L-137) and β-glucan (BG) on digestive enzyme activity and intestinal morphology of genetically improved farmed tilapia (GIFT) with focus on insulin-like growth factor I (IGF-I), fatty acid synthase (FAS), and glucose-6-phosphate dehydrogenase (G6PD). For 12 weeks, fish fed the control, or three diets incorporated with 100 HK L-137, 100 BG, or 50 HK L-137 + 50 BG mg/kg (HK L-137, BG, and HK L-137/BG diets)...
May 11, 2019: Probiotics and Antimicrobial Proteins
Carolina Ramírez, Rodrigo Rojas, Jaime Romero
Seriola lalandi is an economically important species that is globally distributed in temperate and subtropical marine waters. Aquaculture production of this species has had problems associated with intensive fish farming, such as disease outbreaks or nutritional deficiencies causing high mortality. Intestinal microbiota are involved in many processes that benefit a host, such as disease control, stimulation of the immune response, and the promotion of nutrient metabolism. The aim of this study is to evaluate the in vitro probiotic properties of bacteria isolated from the intestinal content of wild Seriola lalandi...
May 10, 2019: Probiotics and Antimicrobial Proteins
Lenka Micenková, Juraj Bosák, Stanislav Smatana, Adam Novotný, Eva Budinská, David Šmajs
Colinfant New Born (CNB) is an orally administered probiotic preparation containing the Escherichia coli strain A0 34/86, which is specially marketed for use in newborns and infants. Although the impact of different probiotics on the composition of the human gut microbiota has been previously described, the effects of E. coli probiotic consumption during infancy on the development of intestinal microbiota are not known. The effect of oral administration of CNB on the Enterobacteriaceae population was mapped using 16S rRNA gene sequencing in DNA samples isolated from the stools of one infant collected at 177 different time points during the first year of life...
May 8, 2019: Probiotics and Antimicrobial Proteins
Natalia Barbieri, Susana Salva, Matías Herrera, Julio Villena, Susana Alvarez
The effect of Lactobacillus rhamnosus CRL1505 (Lr) on macrophages (Ma) and dendritic cells (DC) in the orchestration of anti-pneumococcal immunity was studied using malnutrition and pneumococcal infection mouse models. Monocytes (Mo), Ma, and DC in two groups of malnourished mice fed with balanced diet (BCD) were studied through flow cytometry; one group was nasally administered with Lr (BCD+Lr group), and the other group was not (BCD group). Well-nourished (WNC) and malnourished (MNC) mice were used as controls...
April 27, 2019: Probiotics and Antimicrobial Proteins
Takavar Mohammadian, Zahra Tulaby Dezfuly, Reza Ghanei Motlagh, Abdolhossein Jangaran-Nejad, Seyed Samad Hosseini, Hossein Khaj, Nasim Alijani
The present study was conducted to investigate the effects of probiotic and encapsulated Lactobacillus bulgaricus on hematological and immunological factors after lead toxicity in rainbow trout (Oncorhynchus mykiss). Two hundred and forty fish weighing about 16 ± 3.8 g were divided randomly in to four groups including two groups which were fed by a diet containing ~ 108  CFU g-1 Lactobacillus bulgaricus and encapsulated Lactobacillus bulgaricus bacteria and also the third group diet without Lactobacillus bulgaricus...
April 25, 2019: Probiotics and Antimicrobial Proteins
Abd El-Moneim Eid Abd El-Moneim, Ibrahim El-Wardany, Adel M Abu-Taleb, Magda M Wakwak, Tarek A Ebeid, Ahmed A Saleh
Bifidobacterium is one of the most promising probiotics which was recently used as an alternative growth promoter in poultry. This trial was considered to evaluate the potential effects of in ovo administration of Bifidobacterium bifidum and Bifidobacterium longum on growth performance and biochemical parameters of broilers. Three hundred broiler breeder eggs were incubated and candled individually at day 17 of embryogenesis; then, eggs that had live embryos were randomly divided into six groups. The first and second groups were of intact (without injection) and vehicle-injected (sterile distilled water) control groups, respectively...
April 25, 2019: Probiotics and Antimicrobial Proteins
Seyed Mohammad Ali Aziz Mousavi, Seyed Ali Mirhosseini, Mona Rastegar Shariat Panahi, Hamideh Mahmoodzadeh Hosseini
Silver nanoparticles are the most desirable nanoparticles broadly used in diverse fields. This study intends to investigate the anticancer properties of synthesized silver/Lactobacillus rhamnosus GG nanoparticles (Ag-LNPs) as a reducing and stabilizing agent in the synthesis process. To prepare silver/Lactobacillus rhamnosus GG nanoparticles, 1 mg/ml cell lysate of Lactobacillus rhamnosus GG and 1 mM silver nitrate solution were mixed and incubated for 72 h. XRD, FTIR, and TEM methods were used for nanoparticle characterization...
April 24, 2019: Probiotics and Antimicrobial Proteins
Hichem Moulahoum, Faezeh Ghorbani Zamani, Suna Timur, Figen Zihnioglu
Peptides are considered very important due to the diversity expressed through their amino acid sequence, structure variation, large spectrum, and their essential role in biological systems. Antimicrobial peptides (AMPs) emerged as a potent tool in therapy owing to their antimicrobial properties but also their ability to trespass the membranes, specificity, and low toxicity. They comprise a variety of peptides from which specific amino acid-rich peptides are of interest to the current review due to their features in metal interaction and cell penetration...
April 18, 2019: Probiotics and Antimicrobial Proteins
Lei Wang, Xueqin Zhao, Xiaojing Xia, Chunling Zhu, Wanhai Qin, Yanzhao Xu, Bolin Hang, Yawei Sun, Shijun Chen, Huihui Zhang, Jinqing Jiang, Jianhe Hu, Hanna Fotina, Gaiping Zhang
Salmonella is an important zoonotic pathogen and is a major cause of gastrointestinal diseases worldwide. The current serious problem of antibiotic abuse has prompted the search for new substitutes for antibiotics. JH-3 is a small antimicrobial peptide with broad-spectrum bactericidal activity. In this study, we showed that JH-3 has good bactericidal activity towards the clinical isolate Salmonella enterica serovar Typhimurium strain CVCC541. The minimum inhibitory concentration (MIC) of JH-3 against this bacterium was determined to be 100 μg/mL, which could decrease the number of CVCC541 cells by 1000-fold in vitro within 5 h...
April 18, 2019: Probiotics and Antimicrobial Proteins
Yogeswaran Jagadeesan, Selvamanikandan Athinarayanan, Sabeena Begum Mohamed Ayub, Anandaraj Balaiah
Paenibacillus alvei NP75, a Gram-positive bacterium, produces two different antimicrobial peptides, paenibacillin N and P, which has potent antimicrobial activity against many clinical pathogens. The synthesis pattern of these antimicrobial peptides by P. alvei NP75 was studied extensively. The results were outstanding in a way that the paenibacillin N was synthesized irrespective of the growth of bacteria (non-ribosomal mediated), whereas paenibacillin P production was carried out by ribosomal mediated. In addition to the antimicrobial peptides, P...
April 17, 2019: Probiotics and Antimicrobial Proteins
Torsten Stein
It has been recently shown, that certain strains/isolates of Bacillus subtilis can be used as a probiotic for humans. The production of the macrocyclic sactibiotic subtilosin in B. subtilis ATCC 6633 is highly regulated. To improve the subtilosin productivity of B. subtilis, different growth conditions were compared for maximal expression of the sbo promoter that regulates the expression of the subtilosin biosynthetic gene cluster. Oxygen-limiting conditions led to a strong increase of sbo promoter activities compared to aerobic conditions, and accordingly, the subtilosin amount determined by reversed phase HPLC (7...
April 13, 2019: Probiotics and Antimicrobial Proteins
Houssam Abouloifa, Yahya Rokni, Reda Bellaouchi, Nabil Ghabbour, Salwa Karboune, Milena Brasca, Riadh Ben Salah, Nour Eddine Chihib, Ennouamane Saalaoui, Abdeslam Asehraou
The aim of this work is to characterize the potential probiotic properties of 14 antifungal Lactobacillus strains isolated from traditional fermenting Moroccan green olives. The molecular identification of strains indicated that they are composed of five Lactobacillus brevis, two Lactobacillus pentosus, and seven Lactobacillus plantarum. In combination with bile (0.3%), all the strains showed survival rates (SRs) of 83.19-56.51% at pH 3, while 10 strains showed SRs of 31.67-64.44% at pH 2.5. All the strains demonstrated high tolerance to phenol (0...
March 30, 2019: Probiotics and Antimicrobial Proteins
Ratna Sudha Madempudi, Jayanthi Neelamraju, Jayesh J Ahire, Sandeep K Gupta, Vineet K Shukla
Functional constipation has a high prevalence in both adults and children affecting quality of life. Evidence suggests that probiotics can reduce the symptoms of constipation. As the effects of probiotics are strain specific, the efficacy of Bacillus coagulans Unique IS2 in the treatment of functional constipation in adults was evaluated. Subjects (n = 100) diagnosed with functional constipation were supplemented with either B. coagulans Unique IS2 (2 billion CFU) or placebo capsules once daily for 4 weeks...
March 25, 2019: Probiotics and Antimicrobial Proteins
Yanping Wu, Han Xu, Xuefang Cao, Rongrong Liu, Li Tang, Zhonghua Zeng, Weifen Li
Probiotics have always been considered as a supplementary therapy for many diseases especially gut disorders. The absorption and barrier function of the gut play a vital role in the maintenance of body homeostasis. This study was to investigate the protective effects of Bacillus amyloliquefaciens SC06 (Ba) on H2 O2 -induced oxidative stress on intestinal porcine epithelial cells (IPEC-1) based on the level of gene expression. We demonstrated that Ba was a safe probiotic strain in the first place. Results showed that treatment with H2 O2 significantly increased the mRNA expression of absorptive transporters glucose transporter 2 (GLUT2), Ala/Ser/Cys/Thr transporter 1 (ASCT1), and ASCT2 compared with the control group...
March 20, 2019: Probiotics and Antimicrobial Proteins
Panya Dowdell, Surang Chankhamhaengdecha, Watanalai Panbangred, Tavan Janvilisri, Amornrat Aroonnual
Lactic acid bacteria, Enterococcus faecium and Lactococcus lactis, previously isolated from Thai fermented sausages were elucidated their probiotic properties especially in the control of Clostridium difficile 630. Both isolates survived in simulated gastric solution at pH 3 followed in simulated intestinal solution at pH 8. The presence of skimmed milk also helped the bacteria to survive through acidic and alkaline in gastrointestinal conditions. The adhesion properties of both isolates were tested using a human colon adenocarcinoma cell line...
March 19, 2019: Probiotics and Antimicrobial Proteins
Jianming Zhang, Xue Han, Lanwei Zhang, Huaxi Yi, Shiwei Chen, Pimin Gong
The effects of fructose on plantaricin Q7 biosynthesis in Lactobacillus plantarum (L. plantarum) Q7 were investigated. Fructose induced and accelerated plantaricin Q7 production in the early stages of biosynthesis. Two-dimensional electrophoresis and quantitative real-time reverse transcriptase PCR (qRT-PCR) revealed that shock-related proteins were overexpressed in L. plantarum grown in MRS-fructose compared with cultures grown in MRS broth. A preheat-shock (PHS) treatment was used to overexpress shock-related proteins to enhance plantaricin Q7 production...
March 18, 2019: Probiotics and Antimicrobial Proteins
Mohammad Ali Sabokkhiz, Abbas Tanhaeian, Mojtaba Mamarabadi
Antimicrobial peptides (AMPs) are generally small peptides with less than 50 amino acid residues, which have been considered as the first line of defense system in plants and animals. These small cationic peptides belong to a family of antimicrobials that are multifunctional effectors of innate immunity. The direct antimicrobial activity of AMPs against different bacteria, viruses, fungi, and parasites has been confirmed in different studies. In this study, the antiviral activity of two recombinant AMPs named thanatin and lactoferricin+lactoferrampin was evaluated against Tobacco mosaic virus (TMV) using half-leaf and leaf disk methods under in vivo and in vitro condition, respectively...
March 18, 2019: Probiotics and Antimicrobial Proteins
Chirag Patel, Priyanshi Patel, Sanjeev Acharya
To screen Bacillus clausii UBBC07 as a putative probiotic strain and to examine the protective effect of probiotic-B. clausii UBBC07 spore on uremia on rats induced by acetaminophen. In vitro tests performed to screen potential probiotic strains were gastric and bile acid resistance and ability to reduce pathogen adhesion to surfaces. An in vivo study was performed on rats (n = 18) which were randomly divided into three groups: group I, control-receives normal food and water, groups II and III receive acetaminophen i...
March 16, 2019: Probiotics and Antimicrobial Proteins
Carolina de Oliveira Vogado, Eliana Dos Santos Leandro, Ernandes Rodrigues de Alencar, Verônica Cortez Ginani, Renata Puppin Zandonadi
This study evaluated the in vitro effect of 3.0, 6.0, and 9.0% of green banana pulp (GBP) incorporation in fermented milk on the survival of Lactobacillus paracasei subsp. paracasei LBC 81 subjected to acid stress conditions and in the presence of bile salts. Tolerance to acid stress in pH 2.0 and in the presence of 0.30% of bile salts was evaluated right after the incorporation of the fermented milk in each of these conditions, and also during 3 and 4 h of exposure, respectively. The addition of GBP (3.0%) gives a protective effect on L...
March 8, 2019: Probiotics and Antimicrobial Proteins
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