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Molecular Medicine Reports

Ya Hou, Xiaobo Wang, Xiaorui Chen, Jing Zhang, Xiaopeng Ai, Yusheng Liang, Yangyang Yu, Yi Zhang, Xianli Meng, Tingting Kuang, Yao Hu
This study was conducted to establish a stable hypobaric hypoxia brain injury model. SD rats were randomly separated into control and model groups, and placed outside or inside of a hypobaric chamber, respectively. Subsequent to 24 h anoxic exposure, plasma superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), oxidized glutathione (GSSG) and lactate dehydrogenase (LDH) were measured using commercial biochemical kits. Hematoxylin‑eosin (H&E), Nissl's and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were used to observe the morphology of neurons in the hippocampus...
February 5, 2019: Molecular Medicine Reports
Katsuya Iuchi, Mika Ema, Moe Suzuki, Chikako Yokoyama, Hisashi Hisatomi
Polyunsaturated fatty acids are oxidized by non‑enzymatic or enzymatic reactions. The oxidized products are multifunctional. In this study, we investigated how oxidized fatty acids inhibit cell proliferation in cultured cells. We used polyunsaturated and saturated fatty acids, docosahexaenoic acid (DHA; 22:6), eicosapentaenoic acid (EPA; 20:5), linoleic acid (LA; 18:2), and palmitic acid (16:0). Oxidized fatty acids were produced by autoxidation of fatty acids for 2 days in the presence of a gas mixture (20% O2 and 80% N2)...
February 5, 2019: Molecular Medicine Reports
Liping Guo, Baoli Li, Meijing Miao, Jianjun Yang, Jinshan Ji
Previous studies have demonstrated that numerous tumor‑specific microRNAs (miRNAs) are upregulated or downregulated in hepatocellular carcinoma (HCC), and that their dysregulation is implicated in HCC occurrence and development. Therefore, investigation of crucial miRNAs involved in HCC oncogenesis and progression may provide novel insights into the therapy of patients with this malignant tumor. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) assays were performed to detect tissue and cellular expression levels of miRNA‑663b (miR‑663b) in HCC...
February 5, 2019: Molecular Medicine Reports
Wenbo Zhang, Xiaotong Li, Shanquan Sun, Xi Zhang
Engineered conduction tissues (ECTs) are cardiac conduction tissues fabricated in vitro to allow for more precisely targeted in vivo transplantation therapy. The transplantation of ECTs may be ideal for the treatment of atrioventricular conduction block and could have a significant impact on the future application of biological pacemakers. However, there is little published information regarding the conduction function of ECTs in vivo. In the present study, ECTs were constructed by seeding cardiac progenitor cells (CPCs) into a collagen sponge and were then transplanted into animal hearts to determine whether they could act as an atrioventricular conduction pathway...
February 5, 2019: Molecular Medicine Reports
Mihaela Mitroi, Dana Albulescu, Alina Capitanescu, Anca Oana Docea, Gabriela Musat, George Mitroi, Ovidiu Zlatian, Aristidis Tsatsakis, George Tzanakakis, Demetrios A Spandidos, Daniela Calina
The present study focused on the assessment of the inflammatory infiltrate that characterizes nasal polyps in patients with chronic rhinosinusitis and nasal polyposis. Inflammatory cell type was determined using specific markers. This evaluation was made possible by determining the expression of the following markers: CD20, a marker of B lymphocytes [using activated T cells (ATC) armed with CD20 antibody]; CD3, a marker of T lymphocytes (using ATC armed with anti‑CD3 antibody); CD45, the leukocyte common antigen (using ATC armed with anti‑CD45 antibody; and CD34, for the microvasculature of the nasal polyp (using anti‑CD34 antibody)...
February 5, 2019: Molecular Medicine Reports
Huiling Wu, Xi Song, Yuntao Ling, Jin Zhou, Zhen Tao, Yuying Shen
Non‑alcoholic fatty liver disease (NAFLD) is the most common fatty liver disease in developed countries, in which fat accumulation in the liver is induced by non‑alcoholic factors. The present study was conducted to identify NAFLD‑associated long non‑coding RNAs (lncRNAs), mRNAs and microRNAs (miRNAs). The microarray dataset GSE72756, which included 5 NAFLD liver tissues and 5 controls, was acquired from the Gene Expression Omnibus database. Differentially expressed lncRNAs (DE‑lncRNAs) and mRNAs (DE‑mRNAs) were detected using the pheatmap package...
February 5, 2019: Molecular Medicine Reports
Guangyong Huang, Jingwen Liu, Chuansheng Yang, Youzhang Xiang, Yuehai Wang, Jing Wang, Miaomiao Cao, Wenbo Yang
Dilated cardiomyopathy (DCM) is a common type of non‑ischemic cardiomyopathy, of which the underlying mechanisms have not yet been fully elucidated. Long noncoding RNAs (lncRNAs) have been reported to serve crucial physiological roles in various cardiac diseases. However, the genome‑wide expression profile of lncRNAs remains to be elucidated in DCM. In the present study, a case‑control study was performed to identify expression deviations in circulating lncRNAs between patients with DCM and controls by RNA sequencing...
February 5, 2019: Molecular Medicine Reports
Qiang Ou, Yajuan Zhao, Jianhua Zhou, Xiaolin Wu
Recent studies have revealed that circular RNAs (circRNAs) are involved in the development of various liver diseases. However, the regulatory role of circRNAs in nonalcoholic steatohepatitis (NASH) has not been fully elucidated. In the present study, the circRNA profiles in a NASH mouse model were investigated, and their functions in NASH were predicted using bioinformatics analysis, with the aim of providing novel clues for delineating the mechanisms of action. A NASH mouse model was established by feeding mice with a methionine and choline‑deficient diet...
February 5, 2019: Molecular Medicine Reports
Libo Peng, Zikang Xie, Jie Pei, Bing Wang, Yi Gao, Yuxing Qu
Recent studies have suggested that puerarin may impede osteoclastogenesis and facilitate bone regeneration, in addition to attenuating tissue inflammation. The present study investigated the therapeutic effects of puerarin on inflammatory responses and monocyte recruitment in in vitro and in vivo osteoarthritis (OA) models. Puerarin treatment increased the proliferation of OA chondrocytes, as determined by Cell Counting Kit‑8 assay. In addition, the present results suggested that puerarin suppressed the interleukin‑1β‑induced production of inflammatory cytokines in OA chondrocytes and monocytes/macrophages, as assessed by ELISA...
February 5, 2019: Molecular Medicine Reports
Chang Liu, Xuan Wang, Jiu-Bo Tian, Pei-Yuan Ma, Fan-Xiu Meng, Qi Zhang, Bao-Feng Yu, Rui Guo, Zhi-Zhen Liu, Hai-Long Wang, Jun Xie, Niu-Liang Cheng, Jian-Hua Wang, Bo Niu, Gong-Qin Sun
Earthworms are useful indicator organisms of soil health and Eisenia fetida have been extensively used as test organisms in ecotoxicological studies. In order to gain insight into the gene expression profiles associated with physiological functions of earthworms, a full‑length enriched cDNA library of the Eisenia fetida genome was successfully constructed using Switching Mechanism at 5'End of RNA Template technology. Construction of a cDNA library and analysis of Expressed Sequence Tags (ESTs) are efficient approaches for collecting genomic information and identifying genes important for a given biological process...
February 5, 2019: Molecular Medicine Reports
Yuanshu Zhao, Shoubo Zhang
Phosphoglycerate mutase 1 (PGAM1) is reported to be involved in spermatogenic dysfunction. However, the association between PGAM1 and busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis remains unclear. The aim of the current study was to investigate the association between PGAM1 expression and busulfan‑induced hypospermatogenesis, and the effect of PGAM1 expression on spermatogenic cell apoptosis. PGAM1 expression was detected in mouse models of busulfan‑induced hypospermatogenesis by western blotting, reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry...
February 4, 2019: Molecular Medicine Reports
Qifan Zhang, Shibo Sun, Chen Zhu, Yujian Zheng, Qing Cai, Xiaolu Liang, Haorong Xie, Jie Zhou
The aim of the present study was to identify the differentially expressed genes (DEGs) between primary tumor tissue and adjacent non‑tumor tissue of hepatocellular carcinoma (HCC) samples in order to investigate the mechanisms of HCC. The microarray data of the datasets GSE76427, GSE84005 and GSE57957 were downloaded from the Gene Expression Omnibus database. DEGs were identified using the limma package in the R programming language. Following the intersection of the DEGs screened from the three datasets, 218 genes were selected for further study...
February 4, 2019: Molecular Medicine Reports
Jian-Zhong Li, Tian-Hui Cao, Jin-Cheng Han, Hui Qu, Shuang-Quan Jiang, Bao-Dong Xie, Xiao-Long Yan, Hua Wu, Xiang-Lan Liu, Fan Zhang, Xiao-Ping Leng, Kai Kang, Shu-Lin Jiang
Adipose‑derived stem cells (ADSCs) and bone marrow‑derived stem cells (BMSCs) are considered to be prospective sources of mesenchymal stromal cells (MSCs), that can be used in cell therapy for atherosclerosis. The present study investigated whether ADSCs co‑cultured with M1 foam macrophages via treatment with oxidized low‑density lipoprotein (ox‑LDL) would lead to similar or improved anti‑inflammatory effects compared with BMSCs. ADSCs, peripheral blood monocytes, BMSCs and ox‑LDL were isolated from ten coronary heart disease (CHD) patients...
February 1, 2019: Molecular Medicine Reports
Dong Gao, Xianying Ma, Peiwen Lian, Surong Zhou, Jian Chen
After the publication of the above article, the Editor of Molecular Medicine Reports received an accusation that this paper contained material taken from the PhD thesis of Dr Xiao Bai of the China Academy of Medical Science & Peking Union Medical College, whose thesis entitled "Study on mutations of ECM1 in lipoid proteinosis" was published online in 2015. After having enquired with the authors regarding this allegation, the first author and the corresponding author, Dr Dong Gao, admitted that she had infringed the rights of Dr Bai, and that it was not appropriate that the following figures were published in the research article: Figs...
February 1, 2019: Molecular Medicine Reports
Samar M Koura, Mohamed Salama, Mahmoud El-Hussiny, Mahmoud El-Sayed Awad Khalil, Ahmed Lotfy, Samia Ahmed Hassan, Seham Aly Gad Elhak, Mohamed A Sobh
Selective serotonin reuptake inhibitors are the most commonly prescribed antidepressants worldwide, which have been reported to exert potential detrimental effects on bone mineral density and increase the risk of developing fractures. The present study aimed to investigate the pathways underlying the negative effects of fluoxetine on bone using mesenchymal stem cells (MSCs) derived from rat adipose tissue as a source of osteoprogenitor cells. MSCs were harvested from adipose tissue using a collagenase enzyme digestion method and were allowed to differentiate into osteoprogenitor cells...
February 1, 2019: Molecular Medicine Reports
Bing Chen, Yaluan Ma, Xin Xue, Jie Wei, Gang Hu, Yajun Lin
The present study aimed to assess the protective effects of tetramethylpyrazine (TMP) on the livers of mice fed a high fat diet. The mice were divided into five groups: Regular diet; high fat diet; simvastatin‑treated; and low and high dose TMP‑treated groups. The results demonstrated that, compared with the control group, serum glucose, total cholesterol (TC) and low‑density lipoprotein cholesterol levels were increased in the model group. Additionally, compared with the model group, simvastatin lowered the TC level, whereas TMP did not...
February 1, 2019: Molecular Medicine Reports
Lingrong Zeng, Yu Sun, Xiaolei Li, Jingcheng Wang, Lianqi Yan
10‑Hydroxycamptothecin (HCPT) effectively controls epidural fibrosis, but the exact underlying mechanisms remain ambiguous. Abnormal microRNA (miR)‑23b‑3p expression has been detected in various types of fibrotic tissues that are present in different diseases. The aim of the present study was to elucidate the mechanisms through which HCPT induces fibroblast apoptosis. Reverse transcription‑quantitative polymerase chain reactions were performed on six traumatic scar samples and matched normal skin samples; traumatic scar formation was revealed to be significantly inversely associated with miR‑23b‑3p expression...
February 1, 2019: Molecular Medicine Reports
Xuexia Liu, Qian Li, Wenjuan Wang, Fujun Liu
The energy required for normal sperm function is mainly generated by glycolysis and oxidative phosphorylation. Testis‑specific glycolytic enzymes are expressed in germ cells and present in mature spermatozoa. The objective of the present study was to investigate the association between aberrant expression of glycolytic enzymes and human sperm quality. In silico analysis of glyceraldehyde‑3‑phosphate dehydrogenase, testis‑specific (GAPDHS), phosphoglycerate kinase 2 (PGK2) and lactate dehydrogenase (LDHC) identified that they were exclusively expressed in post‑meiotic germ cells and this was validated in human testes using immunohistochemistry...
February 1, 2019: Molecular Medicine Reports
Lina Wang, Xueman Lyu, Yunqing Ma, Fei Wu, Ling Wang
The dysregulation of microRNAs (miRNAs/miRs) has become increasingly recognized as a primary feature of retinoblastoma (RB). Furthermore, miRNAs have been demonstrated to be involved in the occurrence and development of RB. Therefore, it is crucial to investigate the expression profile and roles of miRNAs in RB in order to identify potential therapeutic targets to treat patients with RB. The expression profile and biological roles of miRNA‑504 (miR‑504) have been reported in numerous types of human cancer; however, the roles of miR‑504 in RB remain unknown...
February 1, 2019: Molecular Medicine Reports
Yoshihito Inoue, Hirokazu Fukui, Xin Xu, Ying Ran, Toshihiko Tomita, Tadayuki Oshima, Jiro Watari, Hiroto Miwa
Gut microbiota plays a pivotal role in not only the gastrointestinal (GI) immune system but also GI motility and metabolism. Antibiotic treatments are likely to affect the gut flora and GI immune system, subsequently disturbing GI motility and body metabolism. In the present study, we investigated antibiotic‑induced alterations of body metabolism and GI motility in association with the macrophage profile in the colon. Specific pathogen‑free (SPF) mice (ICR; 6 weeks old; female) were orally administered vancomycin (0...
January 31, 2019: Molecular Medicine Reports
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