journal
https://read.qxmd.com/read/38346667/quantitative-proteomic-and-metabolomic-profiling-reveals-different-osmoregulation-mechanisms-of-tilapia-cells-coping-with-different-hyperosmotic-stress
#21
JOURNAL ARTICLE
Jingyu Pan, Minxu Wang, Jiahua Zhu, Yuxing Huang, Fan Zhang, Erchao Li, Jianguang Qin, Liqiao Chen, Xiaodan Wang
This study aimed to investigate the different regulatory mechanisms of euryhaline fish under regular hyperosmotic and extreme hyperosmotic stress. The OmB (Oreochromis mossambicus brain) cells were exposed to three treatments: control, regular hyperosmotic stress and extreme hyperosmotic stress. After 12 h exposure, proteomics, metabolomics analyses and integrative analyses were explored. Both kinds of stress lead to lowering cell growth and morphology changes, while under regular hyperosmotic stress, the up-regulated processes related with compatible organic osmolytes synthesis are crucial strategy for the euryhaline fish cell line to survive; On the other hand, under extreme hyperosmotic stress, the processes related with cell apoptosis and cell cycle arrest are dominant...
February 10, 2024: Journal of Proteomics
https://read.qxmd.com/read/38331167/proteomic-and-metabolomic-analysis-of-the-serum-of-patients-with-tick-borne-encephalitis
#22
JOURNAL ARTICLE
YanDan Du, LePing Ou, HaiJun Zheng, DeSheng Lu, YiQing Niu, ChunXi Bao, Meng Zhang, ZhiHui Mi
Tick-borne encephalitis virus (TBEV) is a common virus in Europe and Asia, causing around 10,000 to 10,500 infections annually. It affects the central nervous system and poses threats to public health. However, the exact molecular mechanisms of TBE pathogenesis are not yet fully understood due to the complex interactions between the virus and its host. In this study, a comprehensive analysis was conducted to characterize the serum metabolome and proteome of adult patients infected with TBEV, in comparison to a control group of healthy individuals...
February 6, 2024: Journal of Proteomics
https://read.qxmd.com/read/38331166/oxidation-and-cross-link-of-tyrosine-rich-proteins-are-involved-in-the-periostracum-formation-of-the-green-mussel-perna-viridis-linnaeus
#23
JOURNAL ARTICLE
Jingliang Huang, Feng Lin, Chuang Liu, Maoguo Luo
Ocean acidification causes severe shell dissolution and threats the survival of marine molluscs. The periostracum in molluscs consists of macromolecules such as proteins and polysaccharides, and protects the inner shell layers from dissolution and microbial erosion. Moreover, it serves as the primary template for shell deposition. However, the chemical composition and formation mechanism of the periostracum is largely unknown. In this study, we applied transcriptomic, proteomics, physical, and chemical analysis to unravel the mysteries of the periostracum formation in the green mussel Perna viridis Linnaeus...
February 6, 2024: Journal of Proteomics
https://read.qxmd.com/read/38325732/optimal-conditions-for-carrying-out-trypsin-digestions-on-complex-proteomes-from-bulk-samples-to-single-cells
#24
REVIEW
M Shahid Mansuri, Shveta Bathla, TuKiet T Lam, Angus C Nairn, Kenneth R Williams
To identify proteins by the bottom-up mass spectrometry workflow, enzymatic digestion is essential to break down proteins into smaller peptides amenable to both chromatographic separation and mass spectrometric analysis. Trypsin is the most extensively used protease due to its high cleavage specificity and generation of peptides with desirable positively charged N-and C-terminal amino acid residues that are amenable to reverse phase HPLC separation and MS/MS analyses. However, trypsin can yield variable digestion profiles and its protein cleavage activity is interdependent on trypsin source and quality, digestion time and temperature, pH, denaturant, trypsin and substrate concentrations, composition/complexity of the sample matrix, and other factors...
February 5, 2024: Journal of Proteomics
https://read.qxmd.com/read/38325731/development-of-mass-spectrometry-imaging-on-skeletal-muscle-to-characterize-the-local-pro-inflammatory-and-pro-resolution-lipid-responses-in-a-vaccination-context
#25
JOURNAL ARTICLE
Thomas Ribette, Yannick Charretier, Sebastien Laurent, Patrick Syntin, Emilie Chautard, Xavier Meniche, Marion Darnaud, Frédéric Bequet, Laurent Beloeil, Fabienne Piras-Douce, Josephine Abi-Ghanem
Vaccine reactogenicity is well documented at the clinical level but the mechanism involved at the local or systemic level are still poorly understood. Muscular tissue where most vaccines are administered is the first place of interaction between the vaccine formulation and the host's immune cells. So far, this site of vaccine administration is not well documented from a mechanistic standpoint. The study of early molecular events at the injection site is crucial to understand the local response to vaccines. In this paper, we report a standardized workflow, from the injection of vaccine formulations in rabbit muscle, to the analysis by desorption electrospray ionization and histology staining to understand the role of lipids involved in the inflammation and its resolution on striated muscular tissue...
February 5, 2024: Journal of Proteomics
https://read.qxmd.com/read/38325730/technical-report-the-clinically-useful-selection-of-proteins-protocol-an-approach-to-identify-clinically-useful-proteins-for-validation
#26
JOURNAL ARTICLE
Ella E K Swaney, Stephen Hearps, Paul Monagle, Michael H A Roehrl, Vera Ignjatovic
Clinical proteomics studies aiming to develop markers of clinical outcome or disease typically involve distinct discovery and validation stages, neither of which focus on the clinical applicability of the candidate markers studied. Our Clinically Useful Selection of Proteins (CUSP) protocol proposes a rational approach, with statistical and non-statistical components, to identify proteins for the validation phase of studies that could be most effective markers of disease or clinical outcome. Additionally, this protocol considers commercially available analysis methods for each selected protein to ensure that use of this prospective marker is easily translated into clinical practice...
February 5, 2024: Journal of Proteomics
https://read.qxmd.com/read/38325729/effect-of-feeding-fermented-distiller-s-grains-diets-on-immune-status-and-metabolomics-of-spleen-and-mesenteric-lymph-nodes-in-finishing-cattle
#27
JOURNAL ARTICLE
Shihui Mei, Guangxia He, Tiantian Zhang, Ze Chen, Rong Zhang, Yixiao Liao, Mingming Zhu, Duhan Xu, Yanjuan Shen, Bijun Zhou, Kaigong Wang, Chunmei Wang, Chao Chen, Erpeng Zhu, Zhentao Cheng
Fermented distiller's grains(FDG)are rich in nutrients and can improve the growth performance of domestic animals, which is a high-quality alternative for animal feedstuff. However, there are few reports on the effect of feeding FDG diets on spleen and mesenteric lymph nodes (MLN) immune status and metabolomics in domestic animals. In the present study, eighteen Guanling crossbred cattle (18 months old, 250.0 ± 25 kg) were randomly divided into 3 groups: a basal diet (Control) group, a FDG-15% group, and a FDG-30% group (containing 0%, 15% and 30% FDG to partially replace the concentrates, respectively)...
February 5, 2024: Journal of Proteomics
https://read.qxmd.com/read/38320623/proteomic-profiles-of-leptospira-borgpetersenii-serovar-hardjo-strains-jb197-and-hb203-cultured-at-different-temperatures
#28
JOURNAL ARTICLE
Ellie J Putz, Luis G V Fernandes, Kaitlyn M Sarlo Davila, Julian Whitelegge, John D Lippolis, Jarlath E Nally
Leptospirosis is a global zoonotic disease affecting humans, domestic, and wild animals. Leptospira are typically shed in the urine of reservoir hosts which persist in suitable environments where incidental hosts transmission occurs after direct contact with infected urine or contaminated environments. Interestingly, serologically identical L. borgpetersenii serovar Hardjo strains JB197 and HB203 show divergent disease severity in the hamster model; JB197 causes severe acute infection while HB203 causes persistent chronic infection...
February 4, 2024: Journal of Proteomics
https://read.qxmd.com/read/38316181/proteomic-insight-towards-key-modulating-proteins-regulated-by-the-aryl-hydrocarbon-receptor-involved-in-ovarian-carcinogenesis-and-chemoresistance
#29
JOURNAL ARTICLE
Lubna Therachiyil, Paleerath Peerapen, Shahd M Younis, Aamir Ahmad, Visith Thongboonkerd, Shahab Uddin, Hesham M Korashy
Gynecological malignancies pose a severe threat to female lives. Ovarian cancer (OC), the most lethal gynecological malignancy, is clinically presented with chemoresistance and a higher relapse rate. Several studies have highly correlated the incidence of OC to exposure to environmental pollutants, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a process mainly mediated through activating the aryl hydrocarbon receptor (AhR). We have previously reported that exposure of OC cells to TCDD, an AhR activator, significantly modulated the expression of several genes that play roles in stemness and chemoresistance...
February 4, 2024: Journal of Proteomics
https://read.qxmd.com/read/38290411/meat-omics-trends-and-applications-of-omics-strategies-in-meat-research
#30
EDITORIAL
Mohammed Gagaoua, Daniel Franco, Ranjith Ramanathan
No abstract text is available yet for this article.
January 24, 2024: Journal of Proteomics
https://read.qxmd.com/read/38266913/the-eastern-bandy-bandy-vermicella-annulata-expresses-high-abundance-of-svmp-crisp-and-kunitz-protein-families-in-its-venom-proteome
#31
JOURNAL ARTICLE
Theo Tasoulis, C Ruth Wang, Joanna Sumner, Nathan Dunstan, Tara L Pukala, Geoffrey K Isbister
The Australian elapid snake radiation (Hydrophiinae) has evolved in the absence of competition from other advanced snakes. This has resulted in ecological specialisation in Australian elapids and the potential for venom proteomes divergent to other elapids. We characterised the venom of the Australian elapid Vermicella annulata (eastern bandy bandy). The venom was analysed using a two-dimensional fractionation process consisting of reverse-phase high-performance liquid chromatography then sodium dodecyl sulphate polyacrylamide gel electrophoresis, followed by bottom-up proteomics...
January 22, 2024: Journal of Proteomics
https://read.qxmd.com/read/38246419/profiling-the-neuroproteomics-of-honeybee-brain-a-clue-for-understanding-the-role-of-neuropeptides-in-the-modulation-of-aggressivity
#32
JOURNAL ARTICLE
Gabriela Mendonça Paula, Anally R da Silva Menegasso, José Roberto Aparecido Dos-Santos-Pinto, Osmar Malaspina, Mario Sergio Palma
The aggressivity is modulated in honeybee brain through a series of actions in cascade mode, with the participation of the neuropeptides AmAST A (59-76) and AmTRP (254-262). The aggressivity of honeybees was stimulated by injecting both neuropeptides in the hemocoel of the worker honeybees, which were submitted to behavioral assays of aggression. The brain of stinger individuals were removed by dissection and submitted to proteomic analysis; shotgun proteomic approach of honeybee brain revealed that both neuropeptides activate a series of biochemical processes responsible by production of energy, neuronal plasticity and cell protection...
January 19, 2024: Journal of Proteomics
https://read.qxmd.com/read/38246418/5-ht-treated-mouse-b-cells-alleviate-ulcerative-colitis-via-ripk1-insights-from-proteomic-and-phosphoproteomic-analyses
#33
JOURNAL ARTICLE
Xiuna Zhang, Min Rao, Pujun Gao
5-hydroxytryptamine (5-HT) exerts various physiological effects on the intestine through different signaling pathways and molecular transmission mechanisms, including pro- and anti-inflammatory effects. Adoptive transfer of regulatory B cells (Bregs) into colitis mice has exhibited significant therapeutic benefits. We aimed to elucidate the mechanism through which 5-HT-treated B cells alleviate ulcerative colitis. To this end, we analyzed the proteomic and phosphoproteomic profiles of 5-HT-stimulated B cells from naïve mice...
January 19, 2024: Journal of Proteomics
https://read.qxmd.com/read/38238195/corrigendum-to-determination-of-the-heterogeneous-interactome-between-edwardsiella-tarda-and-fish-gills-journal-of-proteomics-volume-75-issue-4-2-february-2012-pages-1119-1128
#34
Yang Liu, Haili Zhang, Yongjin Liu, Hui Li, Xuanxian Peng
No abstract text is available yet for this article.
January 17, 2024: Journal of Proteomics
https://read.qxmd.com/read/38237666/the-protein-map-of-the-protozoan-parasite-leishmania-leishmania-amazonensis-leishmania-viannia-braziliensis-and-leishmania-leishmania-infantum-during-growth-phase-transition-and-temperature-stress
#35
JOURNAL ARTICLE
Simon Ngao Mule, Joyce Silva Saad, Ismael Pretto Sauter, Livia Rosa Fernandes, Gilberto Santos de Oliveira, Daniel Quina, Fabia Tomie Tano, Deborah Brandt-Almeida, Gabriel Padrón, Beatriz Simonsen Stolf, Martin R Larsen, Mauro Cortez, Giuseppe Palmisano
Leishmania parasites cause a spectrum of diseases termed leishmaniasis, which manifests in two main clinical forms, cutaneous and visceral leishmaniasis. Leishmania promastigotes transit from proliferative exponential to quiescent stationary phases inside the insect vector, a relevant step that recapitulates early molecular events of metacyclogenesis. During the insect blood meal of the mammalian hosts, the released parasites interact initially with the skin, an event marked by temperature changes. Deep knowledge on the molecular events activated during Leishmania-host interactions in each step is crucial to develop better therapies and to understand the pathogenesis...
January 16, 2024: Journal of Proteomics
https://read.qxmd.com/read/38218429/millmeter-wave-irradiation-regulates-mrna-expression-and-the-ubiquitin-proteasome-system-in-wheat-exposed-to-flooding-stress
#36
JOURNAL ARTICLE
Setsuko Komatsu, Takumi Nishiuchi, Takashi Furuya, Masahiko Tani
The irradiation with millimeter-wave (MMW) of wheat seeds promotes root growth under flooding stress; however, its role is not completely clarified. Nuclear proteomics was performed, to reveal the role of MMW irradiation in enhancing flooding tolerance. The purity of nuclear fractions purified from roots was verified. Histone, which is a protein marker for nuclear-purification efficiency, was enriched; and cytosolic ascorbate peroxidase was reduced in the nuclear fraction. The principal-component analysis of proteome displayed that the irradiation of seeds affected nuclear proteins in roots grown under flooding stress...
January 11, 2024: Journal of Proteomics
https://read.qxmd.com/read/38218428/proteomic-and-molecular-analyses-to-understand-the-promotive-effect-of-safranal-on-soybean-growth-under-salt-stress
#37
JOURNAL ARTICLE
Rehana Kausar, Takumi Nishiuchi, Setsuko Komatsu
Safranal is a free radical scavenger and useful as an antioxidant molecule; however, its promotive role in soybean is not explored. Salt stress decreased soybean growth and safranal improved it even if under salt stress. To study the positive mechanism of safranal on soybean growth, a proteomic approach was used. According to functional categorization, oppositely changed proteins were further confirmed using biochemical techniques. Actin and calcium-dependent protein kinase decreased in soybean root and hypocotyl, respectively, under salt stress and increased with safranal application...
January 11, 2024: Journal of Proteomics
https://read.qxmd.com/read/38199305/label-free-comparative-proteomic-analysis-of-pleurotus-eryngii-grown-on-sawdust-bagasse-and-peanut-shell-substrates
#38
JOURNAL ARTICLE
Zihao Li, Cuimin Zhao, Yuanyuan Zhou, Suyue Zheng, Qingxiu Hu, Yajie Zou
The white rot fungi Pleurotus eryngii are environmental microorganisms that can effectively break down lignocellulosic biomass. However, understanding of the mechanisms by which P. eryngii is effective in degrading lignocellulose is still limited. This work aimed to examine the extracellular secretory proteins implicated in the breakdown of lignocellulose in P. eryngii and identify degradation tactics across various cultivation substrates. Thus, a comparative analysis of the secretory proteins based on Nanoliquid chromatography combined with tandem mass spectrometry was conducted among P...
January 8, 2024: Journal of Proteomics
https://read.qxmd.com/read/38237665/proteomic-analysis-of-the-mucus-of-the-photosynthetic-sea-slug-elysia-crispata
#39
JOURNAL ARTICLE
Diana Lopes, Susana S Aveiro, Sónia Cruz, Paulo Cartaxana, Pedro Domingues
Elysia crispata is a tropical sea slug that can retain intracellular functional chloroplasts from its algae prey, a mechanism termed kleptoplasty. This sea slug, like other gastropods, secretes mucus, a viscous secretion with multiple functions, including lubrication, protection, and locomotion. This study presents the first comprehensive analysis of the mucus proteome of the sea slug E. crispata using gel electrophoresis and HPLC-MS/MS. We identified 306 proteins in the mucus secretions of this animal, despite the limited entries for E...
March 15, 2024: Journal of Proteomics
https://read.qxmd.com/read/38288553/lentiviral-gene-therapy-with-igf2-tagged-gaa-normalizes-the-skeletal-muscle-proteome-in-murine-pompe-disease
#40
JOURNAL ARTICLE
Qiushi Liang, Eva C Vlaar, Joon M Pijnenburg, Erikjan Rijkers, Jeroen A A Demmers, Arnold G Vulto, Ans T van der Ploeg, Niek P van Til, W W M Pim Pijnappel
Pompe disease is a lysosomal storage disorder caused by deficiency of acid alpha-glucosidase (GAA), resulting in glycogen accumulation with profound pathology in skeletal muscle. We recently developed an optimized form of lentiviral gene therapy for Pompe disease in which a codon-optimized version of the GAA transgene (LV-GAAco) was fused to an insulin-like growth factor 2 (IGF2) peptide (LV-IGF2.GAAco), to promote cellular uptake via the cation-independent mannose-6-phosphate/IGF2 receptor. Lentiviral gene therapy with LV-IGF2...
January 16, 2024: Journal of Proteomics
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