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Tissue Engineering. Part C, Methods

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https://read.qxmd.com/read/30747045/engineered-human-adipose-derived-stem-cells-inducing-endothelial-lineage-and-angiogenic-response
#1
Amita Ajit, Santhoshkumar Thankayyan Retnabai, Lissy K Krishnan
Biotechnological interventions to engineer favorable biochemical environments that facilitate wound repair through quick attainment of vasculature are of immense demand. Potential of angiogenic factors (AF), notably vascular endothelial growth factor A (VEGF-A) and hypoxia-inducible factor-1α (Hif-1α) and the paracrine role of human adipose derived mesenchymal stromal cells (hADMSCs) in establishing vasculature has been reported. Independent application of AF or hADMSCs has led to clinical failure, pertaining to poor means of delivery, deprived bioavailability and insufficiency to drive adequate angiogenesis, respectively...
February 12, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30747044/optimization-of-crispr-cas9-technology-to-knock-out-genes-of-interest-in-aneuploid-cell-lines
#2
Daniyar Dyikanov, Peter Vasiluev, Karina Rysenkova, Natalia Aleksandrushkina, Peter Tyurin-Kuzmin, Konstantin Kulebyakin, Yury Rubtsov, Anna Shmakova, Maria Evseeva, Alexander Balatskiy, Ekaterina Vladimirovna Semina, Alexandra Rostovtseva, Pavel Igorevich Makarevich, Maxim Karagyaur
Most commonly used cell lines are readily susceptible to genome editing and present a good object for cell models to establish disease-causing genes and find ways to cure diseases. However, karyotype and phenotype heterogeneity between individual cells in such cultures as well as multiplicity of target alleles make generation of desired cell lines via single-cell cloning (used for diploid cells) inapplicable. We designed and tested a simple approach for targeted genome modification of single cells in sizable cell populations, containing multiple karyotype and phenotype variants...
February 12, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30747042/development-and-characterization-of-a-rabbit-model-of-compromised-maxillofacial-wound-healing
#3
Stacey Piotrowski, Lindsay Wilson, Neeraja Dharmaraj, Amani Hamze, Ashley Clark, Ramesh Tailor, Lori Hill, Stephen Lai, F Kurtis Kasper, Simon Young
INTRODUCTION: Tissue engineering technologies aiming to enhance maxillofacial wound healing are often tested in vivo in pre-clinical models that do not necessarily reflect the complexity of the clinical need. The aim of this study was to develop a rabbit model of compromised craniofacial wound healing that more accurately mimics clinical scenarios. MATERIALS AND METHODS: An experimental group of rabbits (n= 10) received fractionated radiation of the mandible totaling 36 Gy...
February 12, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30734646/a-standardized-protocol-of-constructing-individual-tissue-engineered-grafts-for-treating-bone-defect
#4
Junchao Xing, Yanzhu Lu, Xiaobo Zhu, Yigong Cui, Fei Luo, Zhao Xie, Xuehui Wu, Moyuan Deng, Jianzhong Xu, Tianyong Hou
Patient-specific individual tissue-engineered bones (iTEBs) have been recognized as a promising strategy for treating large bone defects. However, current construction protocols of iTEBs vary between lots, lack standardization and quality control, hampering further research and application. This study was aimed to detail a standardized constructing protocol for iTEBs, which can be used for both clinical and experimental purposes. The procedure was designed and described as follows: scaffold preparation, cell isolation and culture, and fabrication of iTEBs...
February 8, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30727864/specific-sensitive-and-stable-reporting-of-human-mesenchymal-stromal-cell-chondrogenesis
#5
Rodolfo E De La Vega, Maximilliano Scheu, Lennart A Brown, Christopher Evans, Elisabeth Ferreira, Ryan Michael Porter
Chondrogenesis is critical to the development and repair of not only articular cartilage but also bone. Preclinical studies suggest that defects in both tissues can be repaired using culture-expanded chondroprogenitor cells, such as mesenchymal stem/stromal cells (MSCs), but directing efficient chondrogenesis by candidate cell populations is an ongoing bottleneck to their clinical application. The goal of this study was to describe a method for the molecular reporting of chondrogenic activity by primary stem/progenitor cells that can complement more labor-intensive, destructive measures...
February 6, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30724134/development-of-modular-dual-perfused-osteochondral-constructs-for-cartilage-repair
#6
Ethan Daley, Jochen Kuttig, Jan P Stegemann
Treatment of full-thickness osteochondral defects is challenging because of the need to integrate reparative materials with two separate tissue types. This study demonstrates a modular tissue engineering approach in which multiphase osteochondral tissues are built from hydrogel microbeads (50-150 µm in diameter) into which mesenchymal stromal cells (MSC) and specific matrix compositions were encapsulated. Chondrogenic microbeads consisted of an agarose base containing a polyelectrolyte complex of chondroitin sulfate and chitosan...
February 6, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30661463/effects-of-electromagnetic-field-on-proliferation-differentiation-and-mineralization-of-mc3t3-cells
#7
Luvita Suryani, Jian Hui Too, Ammar Mansoor Hassanbhai, Feng Wen, Daryl Jimian Lin, Na Yu, Swee Hin Teoh
The steep increasing incidence of bone diseases and fractures provides a commanding impetus and growing demands for bone tissue engineering research. Pulsed electromagnetic fields (PEMFs) have been documented to promote bone fracture healing in non-unions and to enhance the maturation of osteoblastic cell, which is the key element in bone tissues. However, the optimal parameters for PEMF stimulation are still being explored. In this study, we investigated the effects of PEMF treatment on the proliferation, differentiation and mineralization of osteoblast precursor cells MC3T3-E1, to explore the cell growth profile under different PEMF exposure durations (15, 30 and 60 minutes daily) with a magnetic field strength of 0...
January 19, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30648479/determination-of-a-critical-size-threshold-for-volumetric-muscle-loss-in-the-mouse-quadriceps
#8
Shannon Emily Anderson, Woojin M Han, Vunya Srinivasa, Mahir Mohiuddin, Marissa Ruehle, June Young Moon, Eunjung Shin, Cheryl L San Emeterio, Molly E Ogle, Edward A Botchwey, Nick Willett, Young C Jang
Skeletal muscle has a remarkable regenerative capacity; however, after volumetric muscle loss (VML) or a loss of a large portion of the tissue, this regenerative response is diminished and results in chronic functional deficits. The critical size at which muscle will not recover has not yet been established; subsequently, the response of crucial muscle components at the critically sized threshold is unknown. In this study, we set out to determine the threshold for a critically sized muscle defect by creating full-thickness VML injuries of 2, 3, or 4 mm diameter in the mouse quadriceps...
January 16, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30648471/non-invasive-longitudinal-bioluminescence-imaging-of-human-mesoangioblasts-in-bioengineered-esophagi
#9
Claire Crowley, Colin Butler, Carlotta Camilli, Rob Hynds, Krishna K Kolluri, Sam M Janes, Paolo De Coppi, Luca Urbani
Esophageal engineering aims to create replacement solutions by generating hollow organs using a combination of cells, scaffolds and regeneration-stimulating factors. Currently, the fate of cells on tissue-engineered grafts is generally determined retrospectively by histological analyses. Unfortunately, quality-controlled cell seeding protocols for application in human patients are not standard practice. As such, the field requires simple, fast and reliable techniques for non-invasive, highly specific cell tracking...
January 16, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30648458/using-a-3d-collagen-matrix-to-deliver-respiratory-progenitor-cells-to-decellularized-trachea-in-vivo
#10
Nick Hamilton, Rob Hynds, Kate Gowers, Angela Tait, Colin Butler, Colin Hopper, Alan Burns, Martin Birchall, Mark Lowdell, Sam M Janes
Airway epithelial cells are a key barrier to inhaled toxicants, pollutants and infectious agents. During severe epithelial injury and in transplantation settings, it would be desirable to rapidly reintroduce a functional epithelium through the engraftment of exogenous cells or by promoting host regeneration. However, the three-dimensional surface of the airway and the requirement to maintain airflow using stents, which cause mechanical shear at the tracheal surface, are challenges. Here, we investigate a graft composed of human airway epithelial stem/progenitor cells and lung fibroblasts embedded within a supportive collagen matrix, that would be more resistant to mechanical shear than cells exposed directly to the lumen surface...
January 16, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30632461/microwell-scaffolds-using-collagen-iv-and-laminin-111-lead-to-improved-insulin-secretion-of-islets
#11
Elahe Hadavi, Jeroen C H Leijten, Marten A Engelse, Eelco de Koning, Pascal Jonkheijm, Marcel Karperien, Aart Alexander van Apeldoorn
Intrahepatic islet-transplantation is a promising therapy for treatment of type 1 diabetes. During islet isolation, collagenase is used to extract islets from the pancreas, leading to loss of important cell-matrix interactions. Loss of the native pancreatic microenvironment is associated with apoptosis of islet cells, early graft failure, and poor islet function. The islet extracellular matrix is comprised of a specific combination of collagen, laminin, and fibronectin molecules. Reintroducing these molecules has been shown to boost the function, viability, morphology, and proliferation of β-cells...
January 11, 2019: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30560717/99mtc-methyl-diphosphonate-binding-to-mineral-deposits-in-cultures-of-mscs-in-osteogenic-medium
#12
Tobias Grossner, Tobias Gotterbarm, Victor Gerbaudo, Uwe Haberkorn, Myron Spector
Osteogenic differentiation of adult mesenchymal stem cells is a promising method for the therapy of critical size bone defects and other applications, drawing attention to the importance of in vitro assays for its evaluation. While there are standardized protocols to induce osteogenic differentiation in monolayer cultures, there is a lack of validated nondestructive procedures to quantify the osteogenic potential of mesenchymal stem cells directly by accessing the mineral deposition. Here, we present a new method to determine the osteogenic potential of mesenchymal stem cells using the radioactive tracer, 99mTc-MDP, to bind in vitro to newly produced mineral deposits from osteogenic-induced stem cells...
December 18, 2018: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30526420/identification-of-cognitive-and-social-framework-of-tissue-engineering-by-science-mapping-analysis
#13
Antonio Santisteban-Espejo, Fernando Campos, Jesus Chato-Astrain, Daniel Durand-Herrera, Oscar Garcia-Garcia, Antonio Campos, Miguel Angel Martin-Piedra, Jose A Moral-Munoz
In recent decades, tissue engineering (TE) has contributed to a revolution in clinical practice, becoming a cornerstone research area in current health sciences. This article presents a science mapping analysis aimed at elucidating the cognitive and social structures that underlie TE during its development from 1991 to 2016. A total of 41,588 documents retrieved from the Web of Science Core Collection formed the TE literature corpus for this analysis. SciMAT was used to identify terms and concepts directly related to TE, and VOSviewer was used to visualize the global scenario of TE authors, institutions and countries...
December 10, 2018: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30501579/vertical-bone-regeneration-with-synthetic-biomimetic-calcium-phosphate-onto-the-calvaria-of-rats
#14
Alain Hoornaert, Yassine Maazouz, David Pastorino, Carlos Aparicio, Gonzague De Pinieux, Borhane Hakim Fellah, Maria-Pau Ginebra, Pierre Layrolle
Bone regeneration is often required to provide adequate oral rehabilitation before dental implants. Vertical ridge augmentation is the most challenging of all situations and often requires the use of autologous bone grafting. However, autologous bone grafting induces morbidity and the harvestable bone is limited in quantity. Alternatives to autologous bone grafting include bovine bone derived biomaterials which provide good clinical results and synthetic bone substitutes that still fail to provide a reliable clinical outcome...
December 1, 2018: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30501564/primary-mucosal-epithelial-cell-cultivation-a-reliable-and-accelerated-isolation-routine
#15
Heide-Katharina Bauer, Daniela Flesch, Stefan Walenta, Ronald E Unger, Roxana Schwab, Sandra Nezi-Cahn, Annette Hasenburg, Martin Heller, Walburgis Brenner
BACKGROUND: Our aim was to establish a reliable protocol for the isolation of primary human oral mucosa epithelial cells that ensured a successful, efficient and reproducible cell culture for the utilization in subsequent experiments and for tissue engineering purposes. Therefore, we analyzed and evaluated various isolation routines and cultivation protocols. Furthermore, we investigated the functionality of isolated epithelial cells for tissue engineering approaches in an in vitro mucosa equivalent cell culture model system...
December 1, 2018: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30501563/3d-printing-of-drug-loaded-scaffolds-for-antibacterial-and-analgesic-applications
#16
Jinlong Shao, Jingqi Ma, Lili Lin, Bing Wang, John A Jansen, Frank Walboomers, Yi Zuo, Fang Yang
Pneumatic extrusion-based 3D printing can be used to fabricate custom-made scaffolds to restore irregular bone defects. During the 3D printing process, therapeutic agents can be added to the scaffolds. This study aimed to develop a polycaprolactone (PCL) scaffold loaded with Ag3PO4 to prevent infections and lidocaine for pain relief by one-step 3D printing. We hypothesized that the drug release could be controlled by varying the filament diameter of the 3D printed scaffolds. To this end, PCL slurry mixed with different amounts of silver phosphate and lidocaine was printed via differently sized nozzles...
December 1, 2018: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30430922/a-high-throughput-workflow-to-study-remodeling-of-ecm-based-microtissues
#17
Katherine Anne Cummins, Alexandra Leigh Crampton, David K Wood
Changes to the cellular microenvironment are an integral characteristic of numerous pathologies including cancer, fibrosis, and autoimmune disease. Current <i>in vitro</i> methodologies available to study 3D tissue remodeling are ill-suited for high-throughput studies as they are not scalable for large-scale experiments. Combining droplet microfluidics and patterned low-adhesion culture surfaces, we have engineered a workflow to incorporate cell-ECM interactions in a versatile and high-throughput platform that is compatible with existing high-throughput liquid handling systems, enables long-term experiments (>1 month), and is well-suited for traditional and novel biological measurements...
November 15, 2018: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30421648/multimaterial-segmented-fiber-printing-for-gradient-tissue-engineering
#18
Luis Antonio Diaz-Gomez, Brandon T Smith, Panayiotis D Kontoyiannis, Sean M Bittner, Anthony J Melchiorri, Antonios G Mikos
In this work, we present a printing method to fabricate scaffolds consisting of multimaterial segmented fibers. Particularly, we developed a reproducible printing process to create single fibers with multiple discrete compositions and control over the distribution of particulate ceramics-namely hydroxyapatite (HA) and β-tricalcium phosphate (TCP)-within poly(ε-caprolactone)-based composite scaffolds. Tensile testing revealed the mechanical integrity of individual segmented fibers was preserved compared to non-segmented fibers, and microcomputed tomography and thermal analysis confirmed the homogeneous distribution of ceramics incorporated in the fiber compositions...
November 13, 2018: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30412046/human-synovial-msc-good-manufacturing-practices-for-articular-cartilage-regeneration
#19
Tiago Lazzaretti Fernandes, Heitor A Kimura, Carla Cristina Gomes Pinheiro, Kazunori Shimomura, Norimasa Nakamura, José R M Ferreira, Andreas H Gomoll, Arnaldo José Hernandez, Daniela Franco Bueno
BACKGROUND: cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal stem cells (MSCs) may be isolated from various known tissues, including discarded fragments at arthroscopy such as synovial membrane...
November 9, 2018: Tissue Engineering. Part C, Methods
https://read.qxmd.com/read/30412042/a-three-dimensional-cell-culture-method-with-a-micromesh-sheet-and-its-application-to-hepatic-cells
#20
Takeshi Hori, Osamu Kurosawa
In vitro 3D cultures of hepatocytes are increasingly being used to assess human hepatic metabolism and toxicity in drug development. Here, we developed an in vitro 3D cell culture method with a microstructured mesh sheet and applied it to culturing human hepatoma HepG2 cells. The micromesh sheet is constituted of fine mesh strands and apertures that are each much larger than a single cell in size. Proliferating on a micromesh sheet, HepG2 cells spread out in a planar manner and then formed a multilayered cell sheet, so that cell-cell adhesion was dominant over cell-substrate adhesion as being different from 2D cultures...
November 9, 2018: Tissue Engineering. Part C, Methods
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