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Tissue Engineering. Part A

Tahereh Derakhshan, Rudra Bhowmick, James H Meinkoth, Jerry Ritchey, Heather Gappa-Fahlenkamp
Mast cells (MCs) play critical roles in the pathogenesis of IgE- and non-IgE-mediated immune responses, as well as host defense against parasites, bacteria, and viruses. Due to the effect of the extracellular matrix components on the tissue morphogenesis and cell behavior, utilizing a tissue model that mimics MC microenvironmental conditions <i>in vivo</i> has greater relevance for <i>in vitro</i> studies. For this work, MCs were developed within a connective tissue-equivalent model and cell function was examined in response to an allergen...
March 21, 2019: Tissue Engineering. Part A
Sarah Berndt, Antoine Turzi, Brigitte Pittet-Cuénod, Ali Modarressi
Nowadays autologous fibroblast application for skin repair presents an important clinical interest. In most cases, in vitro skin cell culture is mandatory. However, cell expansion using xenogeneic or allogenic culture media presents some disadvantages, such as the risk of infection transmission or slow cell expansion. In this study, we investigated an autologous culture system to expand human skin fibroblast cells in vitro with the patient's own platelet-rich-plasma (PRP). Human dermal fibroblasts were isolated from patients undergoing abdominoplasty and blood was collected to prepare non-activated PRP using the CuteCellTM PRP medical device...
March 21, 2019: Tissue Engineering. Part A
Juliana A Passipieri, Xiao Hu, Ellen Mintz, Jack Dienes, Hannah B Baker, C Hunter Wallace, Silvia S Blemker, George J Christ
Despite medical advances, volumetric muscle loss (VML) injuries to craniofacial muscles represent an unmet clinical need. We report an implantable tissue-engineered construct that leads to substantial tissue regeneration and functional recovery in a preclinical model of VML injury that is dimensionally relevant to unilateral cleft lip repair, and a series of corresponding computational models that provide biomechanical insight into mechanism(s) responsible for the VML-induced functional deficits and recovery following tissue-engineered muscle repair implantation...
March 18, 2019: Tissue Engineering. Part A
Antonios G Mikos, John P Fisher, Katja Schenke-Layland, Heungsoo Shin, John A Jansen, Xiu-Mei Wang, Mary Ann Liebert
EDITORIAL Tissue Engineering is proudly celebrating its 25th year in publication in 2019 and would like to begin this Editorial by personally thanking each and every one of our readers, contributors, reviewers, Editorial Board Members, and TERMIS leadership and membership for your continued support of the journal. Your impactful contributions, constructive reviews, and dedicated advocacy of the publication to your peers and institutional leadership has contributed to the continued success of our global publication and we look forward to celebrating our vast achievements throughout the year and beyond...
March 14, 2019: Tissue Engineering. Part A
Liesbet Geris, Ioannis Papantoniou
Ever since the introduction of the concept of Tissue Engineering, the field has developed and matured from a hype to a proper scientific discipline. Recently, the field is witnessing a reversal of the innovation drivers as it has started to move from a technology-driven science-focused field towards a patient-driven manufacturing-focused one. This evolution is made possible through innovations at the interface between biology and technology, including robust biological building blocks, precise biomanufacturing technologies, in-depth characterization methods and in silico models...
March 12, 2019: Tissue Engineering. Part A
Khairunnisa Ramli, Aminath Ifasha Gasim, Amir Adham Ahmad, Ohnmar Htwe, Nor Hazla Mohammad Haflah, Zhe Kang Law, Shariful Hassan, Amaramalar Selvi Naicker, Sabarul Afian Mokhtar, Hisam Mohammad Ariffin, Azmi Baharuddin, Geok Chin Tan, Ruszymah Binti Haji Idrus, Shalimar Abdullah, Min Hwei Ng
We investigated the efficacy of a muscle-stuffed vein seeded with neural-transdifferentiated human mesenchymal stem cells as an alternative nerve conduit to repair a 15-mm sciatic nerve defect in athymic rats.Other rats received either muscle-stuffed vein conduit alone, commercial polyglycolic acid conduit (Neurotube®), reverse autograft or were left untreated. Motor and sensory functions as well as nerve conductivity were evaluated for 12 weeks, after which the grafts were harvested for histological analyses...
March 8, 2019: Tissue Engineering. Part A
Molly S Shoichet, Marian H Hettiaratchi
Protein delivery presents a powerful strategy to stimulate regeneration of damaged tissues. However, the clinical translation of many promising therapeutic proteins in the research pipeline has been hindered by the inability to localize and control their release within the injury site. The only biomaterials-based strategies that have been approved for controlled protein release clinically are the absorbable collagen sponge for bone morphogenetic protein-2 (BMP-2) delivery to repair large bone defects (Medtronic's Infuse Bone Graft) and poly(lactic-co-glycolic) acid (PLGA) microparticles for sustained human growth hormone (hGH) delivery (Genentech's Nutropin Depot)...
March 8, 2019: Tissue Engineering. Part A
Yuki Murata, Jun-Ichiro Jo, Yasuhiko Tabata
The objective of this study is to prepare cationized gelatin nanospheres (cGNS) incorporating molecular beacon (MB) and a cationized gelatin-MB complex, and evaluate the time period of mRNA visualization. The cGNS with different degradabilities were prepared to incorporate MB. There was no difference in the apparent size and zeta potential between the cGNS incorporating MB and the complex, while the MB release from the complex was faster than that from the cGNS. When mouse mesenchymal stem cells were incubated with the complex and cGNS, the amount of MB internalized into cells and cytotoxicity of complex was higher than those of cGNS...
March 8, 2019: Tissue Engineering. Part A
Dominik Steiner, Gregor Lang, Laura Fischer, Sophie Winkler, Peter Greil, Tobias Fey, Thomas Scheibel, Raymund E Horch, Andreas Arkudas
INTRODUCTION: The surgically induced angiogenesis by means of arteriovenous loops (AV loops) represents a powerful method to significantly enhance vascularization of biomaterials. Concerning Tissue Engineering applications, spider silk is a promising biomaterial with a good biocompatibility and slow biodegradation. The current study aims at investigating vascularization as well as de novo tissue formation of fibrous matrices made of electro- or wet-spun engineered ADF4(C16) spider silks in the rat AV loop model...
March 8, 2019: Tissue Engineering. Part A
Keolebogile Shirley Caroline M Motaung, Franca Nneka Alaribe
Tissue engineering and regenerative medicine therapy is still in a very early phase in the African continent, despite the significant progress that has been made in many parts of the world. Although South Africa's position in the world competitiveness scale has improved, there is an urgent need for a skilled and technically sophisticated work force to be developed in the African continent. The existence of traditional and complementary medicine is known to be a fertile ground source of Western Medicine and Africa is rich with medicinal plants...
March 6, 2019: Tissue Engineering. Part A
Hongxai Cai, Bingbing Wu, Yu Li, Yixiao Liu, Libing Shi, Lin Gong, Yaxian Xia, Boon Chin Heng, Huiling Wu, Hongwei Ouyang, Zhenghua Zhu, XiaoHui Zou
Severe infection and mechanical injury of the uterus may lead to infertility and miscarriage. Currently, there is a lack of effective treatment modality for functional repair of uterine injury. To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1α (rhSDF-1α) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier. A rat model of uterine injury was utilized for this study, which was composed of three groups: blank control, implantation with SF-BC only or SF-BC loaded with rhSDF-1α...
March 6, 2019: Tissue Engineering. Part A
Sunil George, Mehran Abolbashari, Tae-Hyoung Kim, Chao Zhang, Julie Allickson, John D Jackson, Sang Jin Lee, In Kap Ko, Anthony Atala, James J Yoo
Kidney disease is a major medical problem globally. Chronic kidney disease (CKD) is a progressive loss of kidney function. It causes accumulation of waste and fluid in the body, eventually resulting in kidney failure as well as damaging other organs. While dialysis and kidney transplantation have been used as primary treatments for renal disease, dialysis does not restore full renal function, and there is a shortage of donor kidneys for transplantation. Recent advances in cell-based therapies have offered a means to augment and restore renal function...
March 2, 2019: Tissue Engineering. Part A
In Gul Kim, Yanru Wu, Su A Park, Hana Cho, Jun Jae Choi, Seong Keun Kwon, Jung-Woog Shin, Eun-Jae Chung
The use of biomaterials for circumferential esophageal repair is technically challenging in a rat model, and an optimal scaffold implantation technique with nutritional support is essential. The purpose of this study was to investigate the effects of 3D-printed esophageal grafts and bioreactor cultivation on muscle regeneration and re-epithelialization from circumferential esophageal defects in a rat model. Here, we designed an artificial esophagus that can enhance the regeneration of esophageal mucosa and muscle through the optimal combination of a two-layered tubular scaffold and MSC-based bioreactor system...
February 23, 2019: Tissue Engineering. Part A
James Armstrong, Molly Stevens
Technological innovation has been integral to the development of tissue engineering over the last 25 years. Future advances will require the next-generation of tissue engineers to embrace emerging technologies. Here, we discuss four key areas of opportunity in which technology can play a role: biological manipulation, advanced characterization, process automation and personalized medicine. This encompasses key developments in transdifferentiation, gene editing, spatially-resolved -omics and 3D bioprinting. Taken together, we can imagine an idealized future in which computational predictions made by machine learning algorithms are used to programme cells and materials to create personalized tissue constructs within an automated culture system...
February 22, 2019: Tissue Engineering. Part A
Hongfu Wu, Lu Ding, Zhe Zhu, Yuhui Wang, Lini Zeng, Tao Wang, Jiang Luo, Tang-Bin Zou, Rui Li, Xuerong Sun, Guangji Zhou, Xiaoqian Liu
Spinal root avulsion typically leads to massive motoneuron death and severe functional deficits of the target muscles. Multiple pathological factors such as severe neuron loss, induction of inhibitory molecules, and insufficient regeneration are responsible for the poor functional recovery. LINGO-1, a CNS-specific transmembrane protein that is selectively expressed on neurons and oligodendrocytes, serves as a potent negative mediator of axonal regeneration and myelination in CNS injuries and diseases. Although accumulating evidence has demonstrated improvement in axonal regeneration and neurological functions by LINGO-1 antagonism in CNS damage, the possible effects of LINGO-1 in spinal root avulsion remain undiscovered...
February 22, 2019: Tissue Engineering. Part A
Juliana A Passipieri, Jack Dienes, Joseph Frank, Joshua Glazier, Andrew J Portell, Kausnik P Venkatesh, Jacqueline Margaret Bliley, Damian Grybowsky, Benjamin Schilling, Kacey Marra, George Joseph Christ
Severe peripheral nerve injuries have devastating consequences on quality of life in affected patients, and represent a significant unmet medical need. Destruction of nerve fibers results in denervation of targeted muscles, which subsequently undergo progressive atrophy and loss of function. Timely restoration of neural innervation to muscle fibers is crucial to preservation of muscle homeostasis and function. The goal of this study was to evaluate the impact of addition of adipose stem cells (ASCs) to polycaprolactone (PCL) nerve conduit guides on peripheral nerve repair and functional muscle recovery in the setting of a critical size nerve defect...
February 14, 2019: Tissue Engineering. Part A
Marina Pöttler, Anna Fliedner, Julia Bergmann, Linh Katrin Bui, Marina Mühlberger, Christian Braun, Matthias Graw, Christina Janko, Oliver Friedrich, Chistoph Alexiou, Stefan Lyer
Losing one`s ability to speak, due to tissue deficiency at the vocal fold (VF), leads to serious impairment in the quality of life. Until now, there is no successful approach for regenerating the VF. The aim of this work is to show the advantage of magnetic nanoparticles in the generation of scaffold-free 3D VF cell-constructs via magnetic tissue engineering (MTE). Rabbit VF-fibroblasts were used to establish MTE: after cellular uptake of superparamagnetic iron oxide nanoparticles (SPIONs), cells can be controlled with a magnetic field thereby forming solid 3D cell-structures...
February 12, 2019: Tissue Engineering. Part A
Chiharu Sogawa, Takanori Eguchi, Yuka Okusha, Kisho Ono, Kazumi Ohyama, Motoharu Iizuka, Ryu Kawasaki, Yusaku Hamada, Masaharu Takigawa, Norio Sogawa, Kuniaki Okamoto, Ken-Ichi Kozaki
Cancer invasion, metastasis, and therapy resistance are the crucial phenomena in cancer malignancy. The high-expression of matrix metalloproteinase 9 (MMP9) is a biomarker as well as a causal factor of cancer invasiveness and metastatic activity. However, a regulatory mechanism underlying MMP9 expression in cancer is not clarified yet. Additionally, a new strategy for anti-cancer drug discovery is becoming an important clue. In the present study, we aimed (i) to develop a novel reporter system evaluating tumorigenesis, invasiveness, metastasis, and druggability with a combination of three-dimensional (3D) tumoroid model and Mmp9 promoter and (ii) to examine pharmacological actions of anti-cancer medications using this reporter system...
February 8, 2019: Tissue Engineering. Part A
Ethan Nyberg, Ashley Farris, Aine O'Sullivan, Ricardo Rodriguez, Warren L Grayson
Large craniofacial bone defects remain a clinical challenge due to their complex shapes and large volumes. Stem cell-based technologies that deliver osteogenic stem cells have shown remarkable regenerative potential but are hampered by the need for extensive in vitro manipulation prior to implantation. To address this, we explored the bone forming potential of the clinically-relevant stromal vascular fraction (SVF) cells obtained from human lipoaspirate. SVF cells can be isolated for acute use in the operating room and contain a sub-population of adipose-derived stem cells (ASCs) that can develop mineralized tissue...
February 8, 2019: Tissue Engineering. Part A
Isis Côrtes, Renata Akemi Morais Matsui, Mayra Souza Azevedo, Anderson Beatrici, Kléber Luiz Araújo Souza, Guilaume Launay, Frédéric Delolme, José Mauro Granjeiro, Catherine Moali, Leandra Santos Baptista
A stabilized cartilage construct without signs of hypertrophy in chondrocytes is still a challenge. Suspensions of ASCs and cartilage progenitor cells (CPCs) were seeded into micro-molded non-adhesive hydrogel to produce spheroids (scaffold- and serum-free method) characterized by size, immunohistochemistry, fusion and biomechanical properties. After cell dissociation, they were characterized for mesenchymal cell surface markers, cell viability, and quantitative real-time PCR. Both targeted and non-targeted (shotgun mass-spectrometry) analysis were conducted on the culture supernatants...
February 8, 2019: Tissue Engineering. Part A
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