Liang Yu Cui, Bo Yang Liu, Hai Meng Li, Yi Xin Zhu, Yong Heng Zhou, Chang Su, Yin Ping Tian, Hai Tao Xu, Dan Liu, Xiao Ping Li, Yue Ma, Guang Shun Jiang, Huan Liu, Shu Hui Yang, Tian Ming Lan, Yan Chun Xu
Utilization of faeces has long been a popular approach for genetic and ecological studies of wildlife. However, the success of molecular marker genotyping and genome resequencing is often unpredictable due to insufficient enrichment of endogenous DNA in the total faecal DNA that is dominated by bacterial DNA. Here, we report a simple and cheap method named PEERS to predominantly lyse animal cells over bacteria by using sodium dodecyl sulphate so as to discharge endogenous DNA into liquid phase before bacterial DNA...
February 19, 2024: Molecular Ecology Resources