Molecular Plant

No abstract text is available yet for this article.

Receptor-like kinases (RLKs) are the largest signal transduction component in plants, determining how different plants adapt to their ecological environment, resulting in plant-specific ecological niches. Current research on RLKs has focused mainly on a small number of typical RLK members of a few model plants. There is an urgent need to study the composition, distribution, and evolution of RLKs at the holistic level to accelerate the understanding of how RLK assists in the ecological adaptation of different plants...

In summary, we accurately annotated the RLKomes and classified RLK families of 300 plant genomes with chromosome-level assemblies. The RLKdb provides comprehensive information of RLKome, RLK family, and RLKs. We also developed an online tool for genome- and transcriptome-wide identification and classification of RLKs. The valuable resources and tools will aid evolutionary and functional studies in the community.

Constructing inbred lines for self-incompatible species and species with long generation times is challenging, making the use of F1 outcross/segregating populations the main strategy for genetic studies of such species. However, there is a lack of dedicated algorithms/tools for rapid QTL mapping using the F1 populations. To this end, we have designed and developed an algorithm/tool called OcBSA specifically for QTL mapping of F1 populations. OcBSA transforms the four-haplotype inheritance problem from the two heterozygous diploid parents of the F1 population into the two-haplotype inheritance problem common in current genetic studies by removing the two haplotypes from the heterozygous parent that do not contribute to phenotype segregation in the F1 population...

No abstract text is available yet for this article.

No abstract text is available yet for this article.

No abstract text is available yet for this article.

Jasmonates (JAs), a class of lipid-derived stress hormones, play a crucial role across an array of plant physiological processes and stress responses. While JA signaling is generally thought to rely dominantly on the degradation of specific JASMONATE-ZIM DOMAIN (JAZ) proteins by SCFCOI1 , it remains unclear whether other pathways are involved in the regulation of JAZ protein stability. Here, we report that PUB22, a plant U-box type E3 ubiquitin ligase, plays a vital role in the regulation of the resistance against Helicoverpa armigera and other JA responses in tomato...

Soybean is a photoperiod-sensitive short-day crop, whose reproductive period and yield are markedly affected by day length changes. Seed weight is among the key traits determining the soybean yield; however, the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod effect on it remain poorly understood. Here we identify a major locus, SW19, which confers seed weight and classify Dt1, an ortholog of Arabidopsis TFL1 gene governing the soybean growth habit, as the causal gene of the SW19 locus that regulates photoperiod-controlled seed weight in soybean...

No abstract text is available yet for this article.

Plant high-affinity K+ transporters (HKTs) mediate Na+ and K+ uptake, maintain Na+ /K+ homeostasis, and therefore play crucial roles in plant salt tolerance. In this study, we present cryoelectron microscopy structures of HKTs from two classes, class I HKT1;1 from Arabidopsis thaliana (AtHKT1;1) and class II HKT2;1 from Triticum aestivum (TaHKT2;1), in both Na+ - and K+ -bound states at 2.6- to 3.0-Å resolutions. Both AtHKT1;1 and TaHKT2;1 function as homodimers. Each HKT subunit consists of four tandem domain units (D1-D4) with a repeated K+ -channel-like M-P-M topology...

Tomato is an ideal model for studying how plants effectively coordinate specialized metabolites to adapt to environmental change. Among plant metabolites, phenolamides, which are conjugates of hydroxycinnamic acid derivatives and polyamines, play important roles in plant adaptation to abiotic and biotic stresses. However, the molecular mechanisms underlying phenolamides metabolism and regulation in tomato, as well as the effects of domestication in breeding on phenolamide diversity, have not been fully elucidated...

No abstract text is available yet for this article.

Changes in ambient temperature profoundly affect plant growth and performance. Therefore, the molecular basis of plant acclimation to temperature fluctuation is of great interest. In this study, we demonstrate that GLYCINE-RICH RNA-BINDING PROTEIN 7 (GRP7) contributes to cold and heat tolerance mechanisms in Arabidopsis (Arabidopsis thaliana). We show that exposure to a warm temperature can rapidly induce GRP7 condensates in planta, which can be reversed by transfer to a lower temperature. GRP7 undergoes liquid-liquid phase separation (LLPS) in vivo and in vitro...

ATP-binding cassette (ABC) transporters are integral membrane proteins that have been evolved with diverse functions via transport of various substrates. In Arabidopsis, the G subfamily of ABC proteins is particularly abundant and participate in multiple signaling pathways during plant development and stress responses. Here, we revealed two Arabidopsis ABCG transporters, ABCG16 and ABCG25, were engaged in ABA-mediated stress responses and early plant growth through an endomembrane-specific dimerization-coupled transport of ABA and ABA-glucosyl ester (ABA-GE), respectively...

No abstract text is available yet for this article.

The spike architecture of wheat plays a crucial role in determining grain number, making it a key trait to optimize in wheat breeding programs. In this study, through a multi-omic approach, we analyzed the transcriptome and epigenome profiles of the young spike at eight developmental stages, revealing coordinated changes in chromatin accessibility and H3K27me3 abundance during the flowering transition. We constructed a core transcriptional regulatory network (TRN) that drives wheat spike formation, and experimentally validated a multi-layer regulatory module involving TaSPL15, TaAGLG1, and TaFUL2...

Plants are aerobic organisms that rely on molecular oxygen for respiratory energy production. Hypoxic conditions, with oxygen levels ranging between 1% and 5%, usually limit aerobic respiration and affect plant growth and development. Here, we demonstrate that hypoxic microenvironment induced by active cell proliferation during the two-step plant regeneration process intrinsically represses the regeneration competence of callus in Arabidopsis thaliana. Hypoxia-repressed plant regeneration was mediated by the RELATED TO APETALA 2...

Nicotiana tabacum and N. benthamiana are widely used models in plant biology research. However, genomic studies of these species have lagged. Here we report construction of chromosome-level reference genomes of N. benthamiana and N. tabacum with an estimated 99.5% and 99.8% completeness respectively. Sensitive transcription start and termination site sequencing methods were developed and used for accurate gene annotation in N. tabacum. Comparative analyses revealed evidence for the parent origins and chromosome structural changes leading to hybrid genome formation of each species...

Lipid phosphorylation by diacylglycerol kinase (DGK) that produces phosphatidic acid (PA) plays important roles in various biological processes, including stress responses, but the mechanism of action remains elusive. Here we show that DGK5 and its lipid product PA suppress ABA biosynthesis by interacting with ABA DEFICIENT 2 (ABA2), a key ABA biosynthesis enzyme, to negatively modulate plant response to abiotic stress tested in Arabidopsis thaliana. Loss of DGK5 function rendered plants less damaged, whereas its overexpression (OE) enhanced plant damage to water and salt stress, compared to wild type (WT)...