Stephen Rotatori, Yichong Zhang, Kirby Madden-Hennessey, Christina Mohammed, Chi-Han Yang, Jordan Urbani, Prem Shrestha, Joseph Pettinelli, Dong Wang, Xueqi Liu, Qi Zhao
Although several technologies have been developed to isolate cells of interest from a heterogenous sample, clogging and impaired cell viability limit such isolation. We have developed the Enrich TROVO system as a novel, nonfluidic technology to sort live cells. The TROVO system combines imaging-based cell selection and photo-crosslinking of (gelatin methacrylate) gelMA-hydrogel to capture cells. After capture, cells are released by enzymatic digestion of the hydrogel and then retrieved for downstream analysis or further cell culturing...
January 2, 2024: New Biotechnology