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Biotechnology for Biofuels

Evert K Holwerda, Robert S Worthen, Ninad Kothari, Ronald C Lasky, Brian H Davison, Chunxiang Fu, Zeng-Yu Wang, Richard A Dixon, Ajaya K Biswal, Debra Mohnen, Richard S Nelson, Holly L Baxter, Mitra Mazarei, C Neal Stewart, Wellington Muchero, Gerald A Tuskan, Charles M Cai, Erica E Gjersing, Mark F Davis, Michael E Himmel, Charles E Wyman, Paul Gilna, Lee R Lynd
[This corrects the article DOI: 10.1186/s13068-019-1353-7.].
2019: Biotechnology for Biofuels
Seong Hyuk Lee, Min-Sik Kim, Sung Gyun Kang, Hyun Sook Lee
Background: The production of biohydrogen (H2 ) as a promising future fuel in anaerobic hyperthermophiles has attracted great attention because H2 formation is more thermodynamically feasible at elevated temperatures and fewer undesired side products are produced. However, these microbes require anoxic culture conditions for growth and H2 production, thereby necessitating costly and time-consuming physical or chemical methods to remove molecular oxygen (O2 ). Therefore, the development of an O2 -tolerant strain would be useful for industrial applications...
2019: Biotechnology for Biofuels
Hui Guo, Younggy Kim
Background: Microbial electrolysis cells (MECs) can be used for energy recovery and sludge reduction in wastewater treatment. Electric current density, which represents the rate of wastewater treatment and H2 production, is not sufficiently high for practical applications of MECs with real wastewater. Here, a sandwiched electrode-stack design was proposed and examined in a continuous-flow MEC system for more than 100 days to demonstrate enhanced electric current generation with a large number of electrode pairs...
2019: Biotechnology for Biofuels
Donatella Cimini, Lucio Zaccariello, Sergio D'Ambrosio, Licia Lama, Giovanna Ruoppolo, Olimpia Pepe, Vincenza Faraco, Chiara Schiraldi
Background: Due to its wide range of applications in the food, pharmaceutical and chemical fields, microbial synthesis of succinic acid is receiving growing attention, generating already relevant industrial results, as well as fueling constant research for improvements. In order to develop a sustainable process, a special focus is now set on the exploitation and conversion of lignocellulosic biomasses into platform chemicals. Results: In the present work we used Basfia succiniciproducens BPP7 in separated hydrolysis and fermentation experiments with Arundo donax as starting material...
2019: Biotechnology for Biofuels
Chaoyun Chen, Andreas Harst, Wuxin You, Jian Xu, Kang Ning, Ansgar Poetsch
Background: Nannochloropsis oceanica belongs to a large group of photoautotrophic eukaryotic organisms that play important roles in fixation and cycling of atmospheric CO2 . Its capability of storing solar energy and carbon dioxide in the form of triacylglycerol (TAG) of up to 60% of total weight under nitrogen deprivation stress sparked interest in its use for biofuel production. Phenotypes varying in lipid accumulation among an N. oceanica population can be disclosed by single-cell analysis/sorting using fluorescence-activated cell sorting (FACS); yet the phenomenon of single cell heterogeneity in an algae population remains to be fully understood at the molecular level...
2019: Biotechnology for Biofuels
Joana T Cunha, Pedro O Soares, Aloia Romaní, Johan M Thevelein, Lucília Domingues
Background: Xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways have been extensively used to confer xylose assimilation capacity to Saccharomyces cerevisiae and tackle one of the major bottlenecks in the attainment of economically viable lignocellulosic ethanol production. Nevertheless, there is a lack of studies comparing the efficiency of those pathways both separately and combined. In this work, the XI and/or XR/XDH pathways were introduced into two robust industrial S...
2019: Biotechnology for Biofuels
Shraddha Maitra, Atul Narang
Background: Recently, we showed that steady-state continuous cultures of S.   stipitis follow the principles of growth on mixture of two complementary substrates. More precisely, when such cultures are fed with progressively higher concentrations of glucose s f at fixed dilution rate D  = 0.1 h-1 , oxygen mass-transfer coefficient k l a  = 50 h-1 , and oxygen solubility <mml:math xmlns:mml=""> <mml:msubsup> <mml:mi>c</mml:mi> <mml:mrow> <mml:mtext>o</mml:mtext> </mml:mrow> <mml:mrow> <mml:mrow/> <mml:mo>∗</mml:mo> </mml:mrow> </mml:msubsup> </mml:math> , they transition from glucose- to oxygen-limited growth through an intermediate dual-limited regime in which both glucose and oxygen are limiting, and ethanol is produced without loss of glucose...
2019: Biotechnology for Biofuels
Luodong Huang, Baoyan Gao, Manman Wu, Feifei Wang, Chengwu Zhang
Background: Among all organisms tested, Haematococcus pluvialis can accumulate the highest levels of natural astaxanthin. Nitrogen starvation and high irradiance promote the accumulation of starch, lipid, and astaxanthin in H . pluvialis , yet their cell division is significantly retarded. Accordingly, adaptive regulatory mechanisms are very important and necessary to optimize the cultivation conditions enabling an increase in biomass; as well as promoting astaxanthin accumulation by H ...
2019: Biotechnology for Biofuels
Xin Wang, Xuelin Wang, Xiaolu Lu, Chen Ma, Kequan Chen, Pingkai Ouyang
Background: Methanol has attracted increased attention as a non-food alternative carbon source to sugar for biological production of chemicals and fuels. Moreover, the high degree of reduction of methanol offers some advantages in increasing the production yields of NAD(P)H-dependent metabolites. Here, we demonstrate an example of methanol bioconversion with the aim of improving production of NAD(P)H-dependent chemicals in synthetic methylotrophic Escherichia coli . Results: A synthetic methylotrophic E...
2019: Biotechnology for Biofuels
Bo Tian, Tianquan Lu, Yang Xu, Ruling Wang, Guanqun Chen
Background: Ricinoleic acid is a high-value hydroxy fatty acid with broad industrial applications. Hiptage benghalensis seed oil contains a high amount of ricinoleic acid (~ 80%) and represents an emerging source of this unusual fatty acid. However, the mechanism of ricinoleic acid accumulation in H. benghalensis is yet to be explored at the molecular level, which hampers the exploration of its potential in ricinoleic acid production. Results: To explore the molecular mechanism of ricinoleic acid biosynthesis and regulation, H...
2019: Biotechnology for Biofuels
Evert K Holwerda, Robert S Worthen, Ninad Kothari, Ronald C Lasky, Brian H Davison, Chunxiang Fu, Zeng-Yu Wang, Richard A Dixon, Ajaya K Biswal, Debra Mohnen, Richard S Nelson, Holly L Baxter, Mitra Mazarei, Wellington Muchero, Gerald A Tuskan, Charles M Cai, Erica E Gjersing, Mark F Davis, Michael E Himmel, Charles E Wyman, Paul Gilna, Lee R Lynd
Background: The recalcitrance of cellulosic biomass is widely recognized as a key barrier to cost-effective biological processing to fuels and chemicals, but the relative impacts of physical, chemical and genetic interventions to improve biomass processing singly and in combination have yet to be evaluated systematically. Solubilization of plant cell walls can be enhanced by non-biological augmentation including physical cotreatment and thermochemical pretreatment, the choice of biocatalyst, the choice of plant feedstock, genetic engineering of plants, and choosing feedstocks that are less recalcitrant natural variants...
2019: Biotechnology for Biofuels
Nannan Li, Yan Zhang, Hongjun Meng, Shengting Li, Shufeng Wang, Zhongchun Xiao, Peng Chang, Xiaohui Zhang, Qing Li, Liang Guo, Yasuo Igarashi, Feng Luo
Background: In the past few decades, microalgae biofuel has become one of the most interesting sources of renewable energy. However, the higher cost of microalgae biofuel compared to that of petroleum prevented microalgae biofuel production. Therefore, the research on increasing lipid productivity from microalgae becomes more important. The lipid production source, triacylglycerol biosynthesis in microalgae requires short chain fatty acids as substrates, which are synthesized in chloroplasts...
2019: Biotechnology for Biofuels
Benwei Zhu, Fang Ni, Yun Sun, Limin Ning, Zhong Yao
Background: The alginate oligosaccharides have been widely used in agriculture, medicine, and food industries due to their versatile physiological functions such as antioxidant, anticoagulant, and antineoplastic activities. The bifunctional alginate lyases can degrade the alginate polysaccharide more efficiently into alginate oligosaccharides. Therefore, it is crucial to discover new bifunctional alginate lyase for alginate oligosaccharide production. Results: Herein, a novel bifunctional alginate lyase FsAlgB was cloned and identified from deep-sea bacterium Flammeovirga sp...
2019: Biotechnology for Biofuels
Yanjuan Zhang, Min Huang, Jianmei Su, Huayu Hu, Mei Yang, Zuqiang Huang, Dong Chen, Juan Wu, Zhenfei Feng
Background: Due to biomass recalcitrance, including complexity of lignocellulosic matrix, crystallinity of cellulose, and inhibition of lignin, the bioconversion of lignocellulosic biomass is difficult and inefficient. The aim of this study is to investigate an effective and green pretreatment method for overcoming biomass recalcitrance of lignocellulose. Results: An effective mechanical activation (MA) + metal salt (MAMS) technology was applied to pretreat sugarcane bagasse (SCB), a typical kind of lignocellulosic biomass, in a stirring ball mill...
2019: Biotechnology for Biofuels
Jiangfeng Huang, Tao Xia, Guanhua Li, Xianliang Li, Ying Li, Yanting Wang, Youmei Wang, Yuanyuan Chen, Guosheng Xie, Feng-Wu Bai, Liangcai Peng, Lingqiang Wang
Background: Genetic modification of plant cell walls has been implemented to reduce lignocellulosic recalcitrance for biofuel production. Plant glycoside hydrolase family 9 (GH9) comprises endo-β-1,4-glucanase in plants. Few studies have examined the roles of GH9 in cell wall modification. In this study, we independently overexpressed two genes from GH9B subclasses ( OsGH9B1 and OsGH9B3 ) and examined cell wall features and biomass saccharification in transgenic rice plants. Results: Compared with the wild type (WT, Nipponbare ), the OsGH9B1 and OsGH9B3 transgenic rice plants, respectively, contained much higher OsGH9B1 and OsGH9B3 protein levels and both proteins were observed in situ with nonspecific distribution in the plant cells...
2019: Biotechnology for Biofuels
Bo Wu, Han Qin, Yiwei Yang, Guowei Duan, Shihui Yang, Fengxue Xin, Chunyan Zhao, Huanhuan Shao, Yanwei Wang, Qili Zhu, Furong Tan, Guoquan Hu, Mingxiong He
Background: Cellulosic biofuels are sustainable compared to fossil fuels. However, inhibitors, such as acetic acid generated during lignocellulose pretreatment and hydrolysis, would significantly inhibit microbial fermentation efficiency. Microbial mutants able to tolerate high concentration of acetic acid are needed urgently to alleviate this inhibition. Results: Zymomonas mobilis mutants AQ8-1 and AC8-9 with enhanced tolerance against acetic acid were generated via a multiplex atmospheric and room temperature plasma (mARTP) mutagenesis...
2019: Biotechnology for Biofuels
Gaoyang Zhang, Sung-Chul Bahn, Geliang Wang, Yanrui Zhang, Beibei Chen, Yuliang Zhang, Xuemin Wang, Jian Zhao
Background: Soybean oil constitutes an important source of vegetable oil and biofuel. However, high temperature and humidity adversely impacts soybean seed development, yield, and quality during plant development and after harvest. Genetic improvement of soybean tolerance to stress environments is highly desirable. Results: Transgenic soybean lines with knockdown of phospholipase Dα1 ( PLDα1KD ) were generated to study PLDα1' s effects on lipid metabolism and seed vigor under high temperature and humidity conditions...
2019: Biotechnology for Biofuels
Kamonchanock Eungrasamee, Rui Miao, Aran Incharoensakdi, Peter Lindblad, Saowarath Jantaro
Background: Cyanobacteria are potential sources for third generation biofuels. Their capacity for biofuel production has been widely improved using metabolically engineered strains. In this study, we employed metabolic engineering design with target genes involved in selected processes including the fatty acid synthesis (a cassette of accD , accA , accC and accB encoding acetyl-CoA carboxylase, ACC), phospholipid hydrolysis ( lipA encoding lipase A), alkane synthesis ( aar encoding acyl-ACP reductase, AAR), and recycling of free fatty acid (FFA) ( aas encoding acyl-acyl carrier protein synthetase, AAS) in the unicellular cyanobacterium Synechocystis sp...
2019: Biotechnology for Biofuels
Mei-Yuan Zhang, Shuai Zhao, Yuan-Ni Ning, Li-Hao Fu, Cheng-Xi Li, Qi Wang, Ran You, Chen-Ying Wang, Han-Nan Xu, Xue-Mei Luo, Jia-Xun Feng
Background: Raw-starch-digesting glucoamylases (RSDGs) from filamentous fungi have great commercial values in starch processing; however, the regulatory mechanisms associated with their production in filamentous fungi remain unknown. Penicillium oxalicum HP7-1 isolated by our laboratory secretes RSDG with suitable properties but at low production levels. Here, we screened and identified novel regulators of RSDG gene expression in P . oxalicum through transcriptional profiling and genetic analyses...
2019: Biotechnology for Biofuels
Jia Wang, Weijun Lin, Zhongdong Yin, Libing Wang, ShuBin Dong, Jiyong An, Zixin Lin, Haiyan Yu, Lingling Shi, Shanzhi Lin, Shaoliang Chen
Background: Based on our previous studies of 17 Prunus sibirica germplasms, one plus tree with high quality and quantity of seed oils has emerged as novel potential source of biodiesel. To better develop P. sibirica seed oils as woody biodiesel, a concurrent exploration of oil content, FA composition, biodiesel yield and fuel properties as well as prediction model construction for fuel properties was conducted on developing seeds to determine the optimal seed harvest time for producing high-quality biodiesel...
2019: Biotechnology for Biofuels
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