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Journal of Tissue Engineering and Regenerative Medicine

Helena Debiazi Zomer, Gisele Kristina Dos Santos Varela, Priscilla Barros Delben, Diana Heck, Talita da Silva Jeremias, Andrea Gonçalves Trentin
Novel strategies combining cell therapy, tissue engineering and regenerative medicine have been developed to treat major skin wounds. Although mesenchymal stromal cells (MSCs) from different tissues have similar stem cell features, such as self-renewing mesodermal differentiation potential and expression of immunophenotypic markers, they also have distinct characteristics. Therefore, we aimed to characterize the application of MSCs derived from the dermis and adipose tissue (DSCs and ASCs, respectively) in cutaneous wound healing by in vitro approaches...
February 18, 2019: Journal of Tissue Engineering and Regenerative Medicine
Ainara Benavente-Babace, Kristina Haase, Duncan J Stewart, Michel Godin
Endothelial progenitor cells (EPCs) and human mesenchymal stem cells (hMSCs) have shown great regenerative potential to repair damaged tissue; however, their injection in vivo results in low retention and poor cell survival. Early clinical research has focused on cell-encapsulation, to improve viability and integration of delivered cells. However, this strategy has been limited by the inability to reproduce large volumes of standardized microcapsules and the lack of information on cell-specific egress and timed release from hydrogel microcapsules...
February 16, 2019: Journal of Tissue Engineering and Regenerative Medicine
Hongbo Tang, Johanna F A Husch, Yang Zhang, John A Jansen, Fang Yang, Jeroen J J P van den Beucken
The effects of immune cells, in particular macrophages, on the behavior of mesenchymal stem cells (MSCs) have recently gained much attention for MSCs-based tissue-engineered constructs. This study aimed to evaluate the effect of monocytes/macrophages on the osteogenic differentiation of adipose-derived mesenchymal stromal cells (ADMSCs) in three-dimensional (3D) co-cultures. For this, we co-cultured THP-1 monocytes, M1 macrophages or M2 macrophages with ADMSCs on 3D poly (lactic-co-glycolic) acid (PLGA)/polycaprolactone (PCL) scaffolds using osteogenic medium for up to 42 days...
February 16, 2019: Journal of Tissue Engineering and Regenerative Medicine
A K Haudenschild, B E Sherlock, X Zhou, J C Hu, J K Leach, L Marcu, K A Athanasiou
Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents. A non-destructive solution to monitor tissue quality and maturation would greatly reduce costs and accelerate the development of tissue engineered products. The objectives of this study were to 1) determine whether matrix stabilization with exogenous lysyl oxidase like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, 2) evaluate whether label-free, non-destructive, Fluorescence Lifetime Imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, 3) form quantitative relationships between destructive and nondestructive measurements to determine whether the strength of these correlations are sufficient to replace destructive testing methods, and 4) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking...
February 15, 2019: Journal of Tissue Engineering and Regenerative Medicine
Weiwei Wang, Yanni Yu, Yi Jiang, Jing Qu, Longxing Niu, Jicheng Yang, Mingzhong Li
Vascularization remains a critical challenge in dermal tissue regeneration. In this study, a vascular endothelial growth factor (VEGF165) and angiopoietin-1 (Ang-1) dual gene co-expression vector that encoded green fluorescent protein (GFP) was constructed from an Arg-Gly-Asp-modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze-drying method. In vitro, the human endothelial-derived cell line EA.hy926 was infected with adenovirus vectors and then expressed GFP, secreted VEGF165 and Ang-1, and promoted cell proliferation effectively...
February 15, 2019: Journal of Tissue Engineering and Regenerative Medicine
Bin Xia, Guobao Chen, Yang Zou, Li Yang, Jun Pan, Yonggang Lv
The treatment of lengthy peripheral nerve defect is challenging in the field of nerve regeneration. Our previous studies have shown that low-intensity pulsed ultrasound (LIPUS) could promote the proliferation, cell viability, and neural differentiation of induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) and improve the regeneration of damaged peripheral nerve. In this study, the mechanical signal transduction pathway of LIPUS promoting iPSCs-NCSCs proliferation and differentiation was further explored, and the effects of LIPUS combined with iPSCs-NCSCs, perfluorotributylamine (PFTBA), and growth differentiation factor 5 (GDF5) on the repair of peripheral nerve injury were evaluated...
February 15, 2019: Journal of Tissue Engineering and Regenerative Medicine
A Roosens, Y Handoyo, P Dubruel, H Declercq
A significant challenge in the field of tissue engineering is the biofabrication of three-dimensional (3D) functional tissues with direct applications in organ-on-a-chip systems and future organ engineering. Multi-cellular valvular microtissues can be used as building blocks for the formation of larger-scale valvular macrotissues. Yet, for the controlled biofabrication of 3D macrotissues with predefined complex shapes, directed assembly of microtissues through bioprinting is needed. This study aimed to investigate if modified gelatin is an instructive material for valvular microtissues...
February 15, 2019: Journal of Tissue Engineering and Regenerative Medicine
Aishwarya Satish, Purna Sai Korrapati
Nerve injuries are often debilitating as its regeneration occurs in a slow and laborious manner. Remediation of nerve injury is a colossal task as functional restoration in larger gaps seldom occurs due to the complex nerve regeneration mechanism. A nanofiber-based graft material has been fabricated to provide topographical and biochemical cues to encourage neural differentiation. Laminin plays a crucial role in supporting peripheral nerve regeneration and hence aligned polyvinyl cinnamate nanofibers surface-conjugated with laminin-derived cell-adhesion peptides have been fabricated to improve selective neural adhesion and regeneration...
February 15, 2019: Journal of Tissue Engineering and Regenerative Medicine
Dénes B Horváthy, Károly Schandl, Charlotte M Schwarz, Károly Renner, István Hornyák, Bence T Szabó, Eugenia Niculescu-Morzsa, Stefan Nehrer, Csaba Dobó-Nagy, Attila Doros, Zsombor Lacza
Serum albumin coated bone allografts (BoneAlbumin) have successfully supported bone regeneration in various experimental models by activating endogenous progenitors. However, the effect of tissue aging, linked to declining stem cell function, has yet to be explicitly examined within the context of BoneAlbuminꞋs regenerative capacity. Stem cell function was tested with an in vitro attachment assay, which showed that albumin coating increases stem cell attachment on demineralized bone surfaces in an aging cell population...
February 12, 2019: Journal of Tissue Engineering and Regenerative Medicine
Mi Jang, Astrid Kleber, Thomas Ruckelshausen, Ralf Betzholz, Andreas Manz
HepaRG is a bipotent stem cell line that can be differentiated toward hepatocyte-like and biliary-like cells. The entire cultivation process requires one month and relies on the addition of 2 % dimethyl sulfoxide (DMSO) to the culture. Our motivation in this research is to differentiate HepaRG cells (progenitor cells, undifferentiated cells) toward hepatocyte-like cells by minimizing the cultivation time and without using DMSO treatment by instead using a microfluidic device combined with the following strategies: i) comparison of extracellular matrices (matrigel, collagen I), ii) types of flow (one or both sides), and iii) effects of DMSO...
February 12, 2019: Journal of Tissue Engineering and Regenerative Medicine
Cristina Martínez-Ramos, Laura Rodríguez Doblado, Eric López Mocholi, Ana Alastrue-Agudo, Marina Sánchez Petidier, Esther Giraldo, Manuel Monleón Pradas, Victoria Moreno-Manzano
Spinal cord injuries (SCI) result in the loss of sensory and motor function with massive cell death and axon degeneration. We have previously shown that transplantation of spinal cord-derived ependymal progenitor cells (epSPC) significantly improves functional recovery after acute and chronic SCI in experimental models, via neuronal differentiation and trophic glial cell support. Here we propose an improved procedure based on transplantation of epSPC in a tubular conduit of hyaluronic acid (HA) containing poly (lactic acid) (PLLA) fibers creating a biohybrid scaffold...
February 6, 2019: Journal of Tissue Engineering and Regenerative Medicine
Levent Özçakar, Bayram Kaymak, Murat Kara, Ayşen Akinci, Serdar Can Güven
PRP treatment has a potential to become a part of non-surgical approach in CTS as a regenerative method. Platelet-rich plasma (PRP) therapies aim to enhance the self-healing ability of human body, by exposing the injured tissue to a high concentration of autologous growth factors. Nerve tissues also seem to benefit from the regenerative effects of PRP concentrates. The aim of this study is to investigate the possible beneficial effects of platelet-rich plasma (PRP) injection in carpal tunnel syndrome (CTS)...
February 1, 2019: Journal of Tissue Engineering and Regenerative Medicine
G Dwivedi, A Chevrier, C D Hoemann, M D Buschmann
Bone Marrow Stimulation (BMS) improves knee joint function but elicits incomplete repair. Liquid chitosan (CS)-glycerol phosphate/blood clots have been shown to improve BMS-based cartilage repair. Platelet-rich-plasma (PRP) - a rich source of growth factors and cytokines - improves recruitment and chondrogenic potential of subchondral mesenchymal stem cells. We hypothesized that repair response in a rabbit chronic defect model will improve when freeze-dried chitosan/PRP is used to augment BMS. Bilateral trochlear defects created in New Zealand White rabbits were allowed to progress to a chronic stage over 4 weeks...
February 1, 2019: Journal of Tissue Engineering and Regenerative Medicine
Joao N Ferreira, Riasat Hasan, Ganokon Urkasemsin, Kiaw K Ng, Christabella Adine, Sujatha Muthumariappan, Glauco R Souza
Salivary gland (SG) hypofunction and oral dryness can be induced by radiotherapy for head and neck cancers or auto-immune disorders. These are common clinical conditions that involve loss of saliva-secreting epithelial cells. Several oral complications arise with SG hypofunction that interfere with routine daily activities like chewing, swallowing and speaking. Hence, there is a need for replacing these saliva-secreting cells. Recently, researchers have proposed to repair SG hypofunction via various cell-based approaches in three-dimensional (3D) scaffold-based systems...
January 21, 2019: Journal of Tissue Engineering and Regenerative Medicine
Uresha Patel, Laura Macri-Pellizzeri, Kazi M Zakir Hossain, Brigitte E Scammell, David M Grant, Colin A Scotchford, Alex C Hannon, Andrew R Kennedy, Emma R Barney, Ifty Ahmed, Virginie Sottile
Phosphate glasses (PBG) are ideal materials for regenerative medicine strategies since their composition, degradation rates and ion release profiles can easily be controlled. Strontium has previously been found to simultaneously affect bone resorption and deposition. Therefore, by combining the inherent properties of resorbable PBG and therapeutic activity of strontium, these glasses could be used as a delivery device of therapeutic factors for the treatment of orthopaedic diseases such as osteoporosis. This study shows the cytocompatibility and osteogenic potential of phosphate based glasses where CaO is gradually replaced by SrO in the near invert glass system 40P2 O5 ·(16-x)CaO·20Na2 O·24MgO·xSrO (x= 0, 4, 8, 12 and 16 mol %)...
January 21, 2019: Journal of Tissue Engineering and Regenerative Medicine
Maya Schnabel-Lubovsky, Olga Kossover, Sonia Melino, Francesca Nanni, Yeshayahu Talmon, Dror Seliktar
The present investigation explores the microscopic aspects of cell-laden hydrogels at high resolutions, using three-dimensional cell cultures in semi-synthetic constructs that are of very-high water content (>98% water). The study aims to provide an imaging strategy for these constructs, while minimizing artifacts. Constructs of PEG-fibrinogen (PEG-Fb) and fibrin hydrogels containing embedded mesenchymal cells (human dermal fibroblasts) were first imaged by confocal microscopy. Next, high resolution scanning electron microscopy (HR-SEM) was used to provide images of the cells within the hydrogels, at submicron resolutions...
January 18, 2019: Journal of Tissue Engineering and Regenerative Medicine
Jan Adamowicz, Blazej Kuffel, Marta Pokrywczynska, Shane Vontelin Van Breda, Tomasz Drewa
Reconstructive urology is a complex and demanding branch of modern urology. Complicated cases, necessity of microsurgical approach and constant exposure to urine make urinary reconstruction especially difficult. With impaired healing, excessive scarring and recurring fibrosis functional results are still not satisfying. For better, more successful outcomes a novel tissue engineering technology based solutions are being gradually investigated. The use of tissue engineering is the most promising strategy to improve results of reconstructive urology procedures due to possibility of designing organ- specific grafts...
January 18, 2019: Journal of Tissue Engineering and Regenerative Medicine
Razieh Dalirfardouei, Khadijeh Jamialahmadi, Amir Hosein Jafarian, Elahe Mahdipour
Wound healing is a complicated process that contains a number of overlapping and consecutive phases, disruption in each of which can cause chronic non-healing wounds. In the current study, we investigated the effects of exosomes as paracrine factors released from menstrual blood-derived mesenchymal stem cells (MenSCs) on wound healing process in diabetic mice. The exosomes were isolated from MenSCs conditioned media using ultracentrifugation and were characterized by SEM and western blotting assay. A full thickness excisional wound was created on the dorsal skin of each streptozotocin-induced diabetic mouse...
January 18, 2019: Journal of Tissue Engineering and Regenerative Medicine
Ugnė Jonavičė, Virginijus Tunaitis, Karolina Kriaučiūnaitė, Akvilė Jarmalavičiūtė, Augustas Pivoriūnas
Functional impairments of microglia have been recently associated with several neurological conditions. Therefore modulation of anti-inflammatory and phagocytic properties of microglial cells could represent a novel therapeutic approach. In the present study we investigated the effects of extracellular vesicles (EVs) derived from stem cells from the dental pulp of human exfoliated deciduous teeth (SHEDs) on the inflammatory response and functional properties of immortalized human microglial cells. NFκB reporter assays demonstrated that EVs suppressed LPS-induced activation of NFκB signalling pathway in human microglial cells...
January 16, 2019: Journal of Tissue Engineering and Regenerative Medicine
Ryota Chijimatsu, Fumiko Yano, Taku Saito, Masato Kobayashi, Shuichi Hamamoto, Takashi Kaito, Junichi Kushioka, David A Hart, Ung-Il Chung, Sakae Tanaka, Hideki Yoshikawa, Norimasa Nakamura
As an alternative to chondrocytes-based cartilage repair, stem cell-based therapies have been investigated. Specifically, human synovium-derived stem cells (hSSCs) are a promising cell source based on their highly capacities for chondrogenesis, but some methodological improvements are still required towards optimal cartilage regeneration. Recently, a small compound, TD-198946, was reported to promote chondrogenesis of several stem cells, but the effect on hSSCs is still unknown. This study aimed to examine the effects of TD-198946 on chondrocyte differentiation and cartilaginous tissue formation with hSSCs...
January 16, 2019: Journal of Tissue Engineering and Regenerative Medicine
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