Luciana Fraga da Costa Diesel, Bruno Paiva dos Santos, Bruno Corrêa Bellagamba, Angelo Syrillo Pretto Neto, Pedro Bins Ely, Lindolfo da Silva Meirelles, Nance Beyer Nardi, Melissa Camassola
Quantitative real-time PCR can detect variations in gene expression. The identification of the stable reference genes (RGs) is necessary to evaluate the expression of specific genes of interest under various conditions in many cell types, including human adipose-derived stromal cells (hASCs). In this study, we used the algorithms BestKeeper, NormFinder, geNorm, and RefFinder to investigate the stability of 15 potential RGs (B2M, eEF1A1, GAPDH, H2AFZ, HMBS, HPRT1, PGK1, PPIA, RPL5, SDHA, TBP, TKT, TRFC, TUBB, and UBC) in hASCs during control, adipo-, chondro-, and osteogenic differentiation for 28 days...
2015: Journal of Stem Cells