Hiroko Shimazaki, Ayaka Ono, Masako Tsuruga, Aya Ueki, Shiori Koseki-Kuno, Takako Toyoda, Kozue Saito, Kazumi Sawakami, Minoru Kariya, Osamu Segawa, Kazuhiro Nakamura, Michinori Koizuka, Atsushi Kuno
Lectin is a biomolecule that recognizes a specific part of glycans and, thus, has been used widely as a probe for glycoprotein analysis. Owing to the wide repertoire in nature combined with the recent two decades of advances in microarray technology, the multiplexed use of lectins has been widely used for glycan profiling of endogenous proteins. Because protein glycosylation is recognized as being biologically important and is expected to be a reliable disease marker, lectin microarray analysis with highly sensitive detection has been used to discover disease-relevant glycosylation alterations...
March 2020: Current Protocols in Protein Science