Christina Coughlan, Kimberley D Bruce, Olivier Burgy, Timothy D Boyd, Cole R Michel, Josselyn E Garcia-Perez, Vanesa Adame, Paige Anton, Brianne M Bettcher, Heidi J Chial, Melanie Koenigshoff, Elena W Y Hsieh, Michael Graner, Huntington Potter
Exosomes are 50- to 150-nm-diameter extracellular vesicles secreted by all mammalian cells except mature red blood cells and contribute to diverse physiological and pathological functions within the body. Many methods have been used to isolate and analyze exosomes, resulting in inconsistencies across experiments and raising questions about how to compare results obtained using different approaches. Questions have also been raised regarding the purity of the various preparations with regard to the sizes and types of vesicles and to the presence of lipoproteins...
September 2020: Current Protocols in Cell Biology