Read by QxMD icon Read

Future Microbiology

Simon Gaisford
In this exclusive interview, Simon Gaisford discusses his current research into gut dysbiosis and its possible relevance to disease states, as well as the efficacy of probiotic supplementation and its possible use as treatment. This interview was conducted by Ellen Colvin, Commissioning Editor of Future Microbiology. Professor Simon Gaisford is the Head of Department at UCL School of Pharmacy, University College London. He uses isothermal microcalorimetry to monitor the growth and interactions of bacteria, with his research-interest focus on the use of probiotic supplements to improve general health and well-being, ameliorate symptoms of disease and eradicate gut pathogens...
February 13, 2019: Future Microbiology
Sanket S Chiplunkar, Carlos A Silva, Luiz E Bermudez, Lia Danelishvili
AIM: To investigate the formation of Mycobacterium avium membrane vesicles (MVs) within macrophage phagosomes. MATERIALS & METHODS: A phagosome model was utilized to characterize proteomics and lipidomics of MVs. A click chemistry-based enrichment assay was employed to examine the presence of MV proteins in the cytosol of host cells. RESULTS: Exposure to metals at concentrations present in phagosomes triggers formation of bacterial MVs...
February 13, 2019: Future Microbiology
Aryadne L de Almeida, Katiany R Caleffi-Ferracioli, Regiane B de L Scodro, Vanessa P Baldin, Débora C Montaholi, Luiza F Spricigo, Sandra S Nakamura-Vasconcelos, Laíse A Hegeto, Eloísa G Sampiron, Giovana F Costacurta, Diego A Dos S Yamazaki, Gisele de F Gauze, Vera Ld Siqueira, Rosilene F Cardoso
AIM:  To evaluate (i) the in vitro activity of eugenol (EUG) and three derivatives against Mycobacterium tuberculosis (Mtb), nontuberculous mycobacteria (NTM) and other bacteria, (ii) the EUG and antituberculosis drugs combinatory effect and (iii) the EUG and its derivatives cytotoxicity. MATERIALS & METHODS:  Minimum inhibitory concentration of the compounds were determined by resazurin microtiter or broth microdilution assay and the drug interaction between EUG and antituberculosis drugs by resazurin drug combination microtiter...
February 13, 2019: Future Microbiology
Tamara K Kakoschke, Christoph Kleinemeier, Martin C Langenmayer, Frank Ebel
AIM: Immunofluorescence microscopy is a powerful technique to detect surface antigens and study their distribution. Analysis of fungi is often hampered by their weak adherence to glass. We therefore established a novel immunofluorescence staining method to overcome this problem. MATERIALS & METHODS: Fungal material from colonies is bound to adhesive tape and stained with antibodies. RESULTS: The obtained samples had very good optical quality, showing low unspecific background staining and allowing analysis by confocal laser scanning microscopy...
February 13, 2019: Future Microbiology
Shailendra K Saxena, Swatantra Kumar, Vimal K Maurya
No abstract text is available yet for this article.
February 13, 2019: Future Microbiology
Mark Wilcox, Suleiman Al-Obeid, Ana Gales, Roman Kozlov, José A Martínez-Orozco, Flavia Rossi, Sergey Sidorenko, Joseph Blondeau
Methicillin-resistant Staphylococcus aureus (MRSA) remains an important cause of serious infection, for which vancomycin is often recommended as the first-choice antibiotic treatment. Appropriate vancomycin prescribing requires accurate measurement of minimum inhibitory concentrations (MICs) to avoid treatment failure, and yet determination can be challenging due to methodological difficulties associated with susceptibility testing. An International Working Group of infectious disease specialists and clinical/medical microbiologists reached a consensus that empirical MRSA infection therapies should be chosen regardless of the suspected origin of the infecting strain (e...
February 6, 2019: Future Microbiology
Hongxia Bao, Jian-Hua Zhao, Songling Zhu, Shuang Wang, Jianjuan Zhang, Xiao-Yu Wang, Bing Hua, Chang Liu, Huidi Liu, Shu-Lin Liu
AIM: Type VI secretion systems (T6SS) play key roles in bacterial pathogenesis, but their evolutionary features remain largely unclear. In this study, we conducted systematic comparisons among the documented T6SSs in Salmonella and determined their structural diversity, phylogenetic distribution and lineage-specific properties. MATERIALS & METHODS: We screened 295 Salmonella genomes for 13 T6SS core components by hidden Markov models and identified 363 T6SS clusters covering types i1, i2, i3 and i4a...
January 23, 2019: Future Microbiology
Milagros Muthular, Fernanda Bálsamo, Pablo Passero, Virginia Jewtuchowicz, Valeria Miozza, Marcelo Blanco Villalba, María Isabel Brusca, Cristina Pérez
Candida albicans is involved in periodontal disease, which is influenced by sex hormones. AIM: To study the effects of the estrogen antagonist tamoxifen (TAM) on periodontal disease of oncological patients; clinical oral strains of C. albicans. PATIENTS: With periodontitis and breast cancer and other with AIDS were used. MATERIALS & METHODS: Periodontal disease was evaluated by the academy of periodontology procedures and the growth of clinical C...
January 23, 2019: Future Microbiology
Yasaman Sadeghi, Seyed Alireza Salami, Pegah Kananizadeh, Sayed-Hamidreza Mozhgani, Mohammad Reza Pourmand
AIM: We designed a novel approach based on real-time PCR followed by high-resolution melting (HRM) analysis to determine the Staphylococcal cassette chromosome mec (SCCmec) types of methicillin-resistant Staphylococcus aureus  strains, which we compared against the results of conventional multiplex PCR SCCmec typing. METHODS: Multiplex PCR (for ccr and mec gene complexes) was carried out as conventional method. The HRM analysis was then designed using standard strains of each SCCmec type...
January 22, 2019: Future Microbiology
Mariane C Bagatin, Andrew M F Rozada, Franciele A V Rodrigues, Paulo S A Bueno, Jessyka L Santos, Fernanda Canduri, Érika S Kioshima, Flavio A V Seixas, Ernani A Basso, Gisele F Gauze
AIM: Novel 4-methoxy-naphthalene derivatives were synthesized based on hits structures in order to evaluate the antifungal activity against Paracoccidioides spp. METHODS: Antifungal activity of compounds was evaluated against P. brasiliensis and most promising compounds 2 and 3 were tested against eight clinically important fungal species. RESULTS: Compound 3 was the more active compound with MIC 8 to 32 μ for Paracoccidioides spp without toxicity monkey kidney and murine macrophagecells...
January 21, 2019: Future Microbiology
Camilla Rodrigues de Almeida Ribeiro, Luciane Almeida Amado, Renata Santos Tourinho, Lyana Rodrigues Pinto Lima, Juliana Gil Melgaço, Adilson José de Almeida, Leonardo Soares Bastos, Lia Laura Lewis-Ximenez, Vanessa Salete de Paula
AIM: We evaluated the accuracy of a commercial rapid immunochromatographic test (rapid test [RT]) for hepatitis A (HA) diagnosis and epidemiological studies. MATERIALS & METHODS: The accuracy of a RT was evaluated in laboratory and in field conditions. Predictive modeling estimated the test performance in a hypothetical population. RESULTS: The RT showed sensitivities of 66-86%, and specificities of 21-100%, depending on the antibody isotype (IgM or IgG) analyzed and prevalence of infection...
January 21, 2019: Future Microbiology
Suman Sharma, Abhishek Sheoran, Krishna B Gupta, Aparna Yadav, Mandira Varma-Basil, Vishnubhatla Sreenivas, Dhruva Chaudhary, Promod K Mehta
AIM: There is an urgent need to design a reliable diagnostic test for tuberculosis (TB). METHODS: Real-time immuno-PCR (RT-I-PCR) assay was devised for the quantitative detection of a cocktail of mycobacterial MPT64 (Rv1980c) and PstS1 (Rv0934) in TB patients. RESULTS: A broad dynamic range of 0.95 pg/ml-95 ng/ml of MPT64+PstS1 was detected in TB patients. In smear-positive (n = 59) and smear-negative (n = 42) pulmonary TB cases, sensitivities of 93...
January 21, 2019: Future Microbiology
Yinchu Zhu, Wenyang Dong, Jiale Ma, Yue Zhang, Zihao Pan, Huochun Yao
AIM: To develop a markerless gene deletion strategy in Streptococcus suis to solve the problem that several serotypes against electrotransformation of foreign DNA. MATERIALS & METHODS: Bioinformatics retrieval was performed to identified ComRS systems functioning for natural transformation. A sacB-spc cassette with the upper and lower homologous fragments was amplification by fusion-PCR for spectinomycin-positive and sucrose-negative selection during gene deletion...
January 21, 2019: Future Microbiology
Toon Peeters, John Penders, Sanne P Smeekens, Gianluca Galazzo, Bert Houben, Mihai G Netea, Paul Hm Savelkoul, Inge C Gyssens
AIM: We aimed to study the mucosal microbiota of the appendix in a prospective appendicitis cohort and to compare the fecal microbiota of patients and controls. We hypothesized that the microbiota may be associated with susceptibility to appendicitis. PATIENTS & METHODS: The fecal microbiota of 99 patients and 106 controls were characterized using 16S-23S intergenic spacer profiling. Richness, diversity and community structure were compared. The appendiceal microbiota from 90 patients was analyzed according to the severity of appendicitis...
January 21, 2019: Future Microbiology
Katiany R Caleffi-Ferracioli, Rosilene F Cardoso, João Vp de Souza, Letícia S Murase, Pedro H Canezin, Regiane Bl Scodro, Vera Lúcia D Siqueira, Fernando R Pavan
AIM: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. MATERIALS & METHODS: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. RESULTS: VP showed good combinatory effect among RIF-resistant isolates...
January 16, 2019: Future Microbiology
Robert Selegård, Amani Musa, Pontus Nyström, Daniel Aili, Torbjörn Bengtsson, Hazem Khalaf
AIM: Bacteriocins are considered as promising alternatives to antibiotics against infections. In this study, the plantaricins (Pln) A, E, F, J and K were investigated for their antimicrobial activity against Staphylococcus epidermidis. MATERIALS & METHODS: The effects on membrane integrity were studied using liposomes and viable bacteria, respectively. RESULTS: We show that PlnEF and PlnJK caused rapid and significant lysis of S. epidermidis, and induced lysis of liposomes...
January 16, 2019: Future Microbiology
Sam Van Hecke, Bas Calcoen, Katrien Lagrou, Johan Maertens
Cytomegalovirus (CMV) manifestations remain important complications after allogeneic hematopoietic stem cell transplantation (HSCT), even in the current era. Unfortunately, available anti-CMV agents, mainly viral polymerase inhibitors, have a substantial risk of myelosuppression and nephrotoxicity. Letermovir, a novel anti-CMV drug that targets the viral terminase complex, has recently been approved for the prevention of clinically significant CMV infection in adult CMV seropositive hematopoietic stem cell transplantation recipients...
January 15, 2019: Future Microbiology
Indran Balakrishnan, Vasiliki Koumaki, Athanasios Tsakris
No abstract text is available yet for this article.
January 15, 2019: Future Microbiology
Carla T Toro, Eva Eliassen, Bhupesh K Prusty
No abstract text is available yet for this article.
January 15, 2019: Future Microbiology
Rita Murri, Eleonora Taddei, Roberto Cauda, Massimo Fantoni
No abstract text is available yet for this article.
January 15, 2019: Future Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"