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Regenerative Medicine | Page 2

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https://read.qxmd.com/read/31313975/characterization-of-an-umbilical-cord-blood-sourced-product-suitable-for-allogeneic-applications
#21
Mukta S Sane, Huiyuan Tang, Neha Misra, Xinzhu Pu, Sara Malara, Christopher D Jones, Soumyajit Banerjee Mustafi
Aim: Umbilical cord blood (UCB) sourced allografts are promising interventions for tissue regeneration. As applications of these allografts and regulations governing them continue to evolve, we were prompted to identify parameters determining their quality, safety and regenerative potential. Materials & methods: Flow-cytometry, mass-spectrometry, protein multiplexing, nanoparticle tracking analysis and standard biological techniques were employed. Results: Quality attributes of a uniquely processed UCB-allograft (UCBr) were enumerated based on identity (cell viability, immunophenotyping, proteomic profiling, and quantification of relevant cytokines); safety (bioburden and microbiological screening), purity (endotoxin levels) and potency (effect of UCBr on chondrocytes and mesenchymal stem cells derived exosomes)...
July 17, 2019: Regenerative Medicine
https://read.qxmd.com/read/31313645/the-effect-of-cartilage-extracellular-matrix-particle-size-on-the-chondrogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells
#22
Chenjun Zhai, Xiao Zhang, Jun Chen, Jian He, Hao Fei, Yang Liu, Chunyang Luo, Weimin Fan
Aim: To investigate the effect of cartilage extracellular matrix (ECM) particle size on the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Materials & methods: BMSCs were seeded into the scaffolds fabricated by small particle ECM materials and large particle ECM materials. For the positive control, chondrogenically induced BMSCs were seeded into commercial poly-lactic-glycolic acid scaffolds. Macroscopic observation, histological and immunohistochemical staining, mechanical testing and biochemical analysis were performed to the cell-scaffold constructs...
July 17, 2019: Regenerative Medicine
https://read.qxmd.com/read/31313635/potential-for-stem-cell-derived-biologic-pumps-for-cardiovascular-and-other-medical-therapies
#23
Robert A Kloner, Sharon L Hale, Wangde Dai
No abstract text is available yet for this article.
July 17, 2019: Regenerative Medicine
https://read.qxmd.com/read/31313632/university-of-wisconsin-solution-for-the-xeno-free-storage-of-adipose-tissue-derived-microvascular-fragments
#24
Matthias W Laschke, Alexander Heß, Claudia Scheuer, Philipp Karschnia, Elena Kontaxi, Michael D Menger
Aim: Adipose tissue-derived microvascular fragments (ad-MVF) are vascularization units for regenerative medicine. We investigated whether University of Wisconsin (UW) solution is suitable for their xeno-free storage. Materials & methods: Murine ad-MVF were cultivated for 24 h in 4°C or 20°C UW solution and 20°C endothelial cell growth medium (control). The ad-MVF were seeded onto collagen-glycosaminoglycan scaffolds, which were analyzed in dorsal skinfold chambers by intravital fluorescence microscopy and histology...
July 17, 2019: Regenerative Medicine
https://read.qxmd.com/read/31269865/industry-updates-from-the-field-of-stem-cell-research-and-regenerative-medicine-in-may-2019
#25
Dusko Ilic, Mirjana Liovic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in May 2019.
July 4, 2019: Regenerative Medicine
https://read.qxmd.com/read/31210581/the-management-of-risk-and-investment-in-cell-therapy-process-development-a-case-study-for-neurodegenerative-disease
#26
Sujith Sebastian, Paul Hourd, Amit Chandra, David J Williams, Nicholas Medcalf
Cell-based therapies must achieve clinical efficacy and safety with reproducible and cost-effective manufacturing. This study addresses process development issues using the exemplar of a human pluripotent stem cell-based dopaminergic neuron cell therapy product. Early identification and correction of risks to product safety and the manufacturing process reduces the expensive and time-consuming bridging studies later in development. A New Product Introduction map was used to determine the developmental requirements specific to the product...
June 18, 2019: Regenerative Medicine
https://read.qxmd.com/read/31204905/investigation-mechanisms-between-normal-developing-and-regenerating-livers-for-regenerative-liver-drug-design
#27
Shang-Wen Wong, Shan-Ju Yeh, Cheng-Wei Li, Lily Hui-Ching Wang, Bor-Sen Chen
Aim: A systematic multimolecule drug design procedure is proposed for promoting hepatogenesis and liver regeneration. Materials & methods: Genome-wide microarray data including three hepatic conditions are obtained from the GEO database (GSE15238). System modeling and big data mining methods are used to construct real genome-wide genetic-and-epigenetic networks (GWGENs). Then, we extracted the core GWGENs by applying principal network projection on real GWGENs of normal, developing and regenerating livers, respectively...
June 17, 2019: Regenerative Medicine
https://read.qxmd.com/read/31187701/utilizing-platelet-rich-fibrin-bioscaffold-at-the-graft-site-improves-the-structure-and-function-of-mice-ovarian-grafts
#28
Elham Shojafar, Malek Soleimani Mehranjani, Seyed Ma Shariatzadeh
Aim: The effect of platelet-rich fibrin (PRF) bioscaffold on the structure and function of mice-autotransplanted ovaries was investigated. Materials & methods: Mice were divided into three groups: control, autografted and autografted + PRF bioscaffold. Angiogenesis, ovary histology and serum biochemical factors were assessed. Results: The total volume of the ovary, the number of follicles and the level of superoxide dismutase activity, total antioxidant capacity, IL-10, progesterone and estradiol were significantly higher in the autografted + PRF bioscaffold group compared with the autografted group...
June 12, 2019: Regenerative Medicine
https://read.qxmd.com/read/31180294/poly-%C3%AE%C2%B5-caprolactone-and-polyvinyl-alcohol-electrospun-wound-dressings-adhesion-properties-and-wound-management-of-skin-defects-in-rabbits
#29
Matej Buzgo, Martin Plencner, Michala Rampichova, Andrej Litvinec, Eva Prosecka, Andrea Staffa, Martin Kralovic, Eva Filova, Miroslav Doupnik, Vera Lukasova, Karolina Vocetkova, Jana Anderova, Tereza Kubikova, Robert Zajicek, Frantisek Lopot, Karel Jelen, Zdenek Tonar, Evzen Amler, Radek Divin, Fabrizio Fiori
Aim: This study evaluates the effect of electrospun dressings in critical sized full-thickness skin defects in rabbits. Materials & methods: Electrospun poly-ε-caprolactone (PCL) and polyvinyl alcohol (PVA) nanofibers were tested in vitro and in vivo . Results: The PCL scaffold supported the proliferation of mesenchymal stem cells, fibroblasts and keratinocytes. The PVA scaffold showed significant swelling, high elongation capacity, limited protein adsorption and stimulation of cells. Nanofibrous dressings improved wound healing compared with the control group in vivo ...
June 10, 2019: Regenerative Medicine
https://read.qxmd.com/read/31180275/early-natural-history-of-neotissue-formation-in-tissue-engineered-vascular-grafts-in-a-murine-model
#30
James W Reinhardt, Juan de Dios Ruiz Rosado, Jenny C Barker, Yong-Ung Lee, Cameron A Best, Tai Yi, Qiang Zeng, Santiago Partida-Sanchez, Toshiharu Shinoka, Christopher K Breuer
Aim: To characterize early events in neotissue formation during the first 2 weeks after vascular scaffold implantation. Materials & methods: Biodegradable polymeric scaffolds were implanted as abdominal inferior vena cava interposition grafts in wild-type mice. Results: All scaffolds explanted at day 1 contained a platelet-rich mural thrombus. Within the first few days, the majority of cell infiltration appeared to be from myeloid cells at the peritoneal surface with modest infiltration along the lumen...
June 10, 2019: Regenerative Medicine
https://read.qxmd.com/read/31170883/industry-updates-from-the-field-of-stem-cell-research-and-regenerative-medicine-in-april-2019
#31
Dusko Ilic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in April 2019.
June 7, 2019: Regenerative Medicine
https://read.qxmd.com/read/31115268/screen-enrich-combine-circulating-system-to-prepare-msc-%C3%AE-tcp-for-bone-repair-in-fractures-with-depressed-tibial-plateau
#32
Wenxiang Chu, Xin Wang, Yaokai Gan, Yifu Zhuang, Dingwei Shi, Fengxiang Liu, Yuehua Sun, Jie Zhao, Tingting Tang, Kerong Dai
Aim: To evaluate the clinical efficacy of mesenchymal stem cell/β-tricalcium phosphate composites (MSC/β-TCP) prepared with a screen-enrich-combine circulating system (SECCS) in patients with depressed tibial plateau fractures. Materials & methods: Bone defects in depressed tibial plateaus were filled with MSC/β-TCP (n = 16) or with β-TCP only (n = 23). Enrichment efficiency and effect of enrichment on cell viability were evaluated. Clinical results were assessed by imaging examination and Lysholm score...
May 22, 2019: Regenerative Medicine
https://read.qxmd.com/read/31115266/nomenclature-and-heterogeneity-consequences-for-the-use-of-mesenchymal-stem-cells-in-regenerative-medicine
#33
Alison Wilson, Andrew Webster, Paul Genever
Mesenchymal stem cells (MSCs) are in development for many clinical indications, based both on 'stem' properties (tissue repair or regeneration) and on signaling repertoire (immunomodulatory and anti-inflammatory effects). Potential conflation of MSC properties with those of tissue-derived stromal cells presents difficulties in comparing study outcomes and represents a source of confusion in cell therapy development. Cultured MSCs demonstrate significant heterogeneity in clonogenicity and multi-lineage differentiation potential...
May 22, 2019: Regenerative Medicine
https://read.qxmd.com/read/31115264/chimeric-feeders-of-mesenchymal-stromal-cells-and-stromal-cells-modified-with-constitutively-active-akt-expand-hematopoietic-stem-cells
#34
Anuradha Vaidya, Shweta Singh, Lalita Limaye, Vaijayanti Kale
Aim: To examine whether AKT-modified stromal cells expand human CD34+ hematopoietic stem cells (HSCs). Methods: Coculture, in vitro functional assays, immuno-fluorescence microscopy, flow cytometry. Results: M2-10B4 stromal cells (M2) modified with AKT1 (M2-AKT) expanded primitive CD34+ 38- HSCs, but affected their functionality. A chimeric feeder layer comprising naive human bone marrow-derived mesenchymal stromal cells and M2-AKT not only overcame the negative effects of M2-AKT, but, unexpectedly, also gave a synergistic effect on the growth and functionality of the HSCs...
May 22, 2019: Regenerative Medicine
https://read.qxmd.com/read/31115261/generation-and-comprehensive-characterization-of-induced-pluripotent-stem-cells-for-translational-research
#35
Chad C MacArthur, Suman Pradhan, Nichole Wetton, Aryan Zarrabi, Carl Dargitz, Mahalakshmi Sridharan, Stephen Jackson, Loni Pickle, Uma Lakshmipathy
Induced pluripotent stem cells (iPSCs) hold immense potential in disease modeling, drug discovery and regenerative medicine. Despite advances in reprogramming methods, generation of clinical-grade iPSCs remains a challenge. Reported here is the first off-the-shelf reprogramming kit, CTS CytoTune-iPS 2.1, specifically designed for clinical and translational research. Workflow gaps were identified, and methods developed were used to consistently generate iPSC from multiple cell types. Resulting clones were subjected to characterization that included confirmation of pluripotency, preservation of genomic integrity and authentication of cell banks via an array of molecular methods including high resolution microarray and next-generation sequencing...
May 22, 2019: Regenerative Medicine
https://read.qxmd.com/read/31115259/freeze-dried-versus-fresh-platelet-rich-plasma-in-acute-wound-healing-of-an-animal-model
#36
Stephany C Huber, José Luiz R C Junior, Letícia Q Silva, Silmara A L Montalvão, Joyce M Annichino-Bizzacchi
Aim: To compare freeze-dried and fresh platelet-rich plasma (PRP) preparations, in a pre-clinical study. Materials & methods:  30 Wistar male rats were used to compare and characterize human PRP which was applied at the perilesional area in an acute wound model, evaluated by macroscopical and histological analysis. Results: Despite the increased growth factor concentration after the freeze-drying process, no change in the healing kinetics was observed in vivo . Nevertheless, a significant increased number of myofibroblasts was demonstrated in comparison with the fresh PRP group...
May 22, 2019: Regenerative Medicine
https://read.qxmd.com/read/31115258/cardiac-nonmyocyte-subpopulations-a-secular-congregation
#37
Mark A Sussman
No abstract text is available yet for this article.
May 22, 2019: Regenerative Medicine
https://read.qxmd.com/read/31115255/g-csf-enhances-the-therapeutic-potency-of-stem-cells-transplantation-in-spinal-cord-injured-rats
#38
Cheng-Yoong Pang, Kuo-Liang Yang, Chin-Hua Fu, Li-Yi Sun, Shin-Yuan Chen, Chia-Hsin Liao
Aim: The therapeutic effects of human wisdom teeth-derived neuronal stem cell (tNSC) cotreatment with granulocyte-colony-stimulating factor (G-CSF) were evaluated for contusion-induced spinal cord injury in rats. Materials & methods:  7 days after contusion, tNSCs were transplanted to the injury site and followed by G-CSF cotreatment for 5 days. Behavioral deficits were evaluated by the Basso, Beattie and Bresnahan test. The injury site was collected for immunohistochemistry analysis. Results: The Basso, Beattie and Bresnahan test significantly improved in the cotreated group compared with the tNSCs or G-CSF single treatment groups...
May 22, 2019: Regenerative Medicine
https://read.qxmd.com/read/31115248/circulatory-support-and-stem-cell-therapy-in-the-management-of-advanced-heart-failure-a-concise-review-of-available-evidence
#39
Mohsin A Hussain, Martina Colicchia, Jessry Veerapen, Deshan Weeraman, Mihai-Nicolae Podaru, Daniel Jones, Ken Suzuki, Anthony Mathur
Stem cell therapy utilizing bone marrow mononuclear cells (BMC's) is a potential strategy to treat heart failure patients with improvement in symptom profile and cardiac function. We describe a rationale for concurrent BMC and left ventricular assist device therapy in selected heart failure patients. This combination therapy has demonstrated improved myocardial perfusion and cardiac function in patients with advanced ischemic cardiomyopathy. Moreover, preclinical data support improved cell retention with left ventricular unloading...
May 22, 2019: Regenerative Medicine
https://read.qxmd.com/read/31074319/human-umbilical-cord-blood-cells-restore-vascular-integrity-in-injured-rat-brain-and-modulate-inflammation-in-vitro
#40
Amit K Srivastava, Karthik S Prabhakara, Daniel J Kota, Supinder S Bedi, Fabio Triolo, Katherine S Brown, Matthew L Skiles, Heather L Brown, Charles S Cox, Scott D Olson
Aim: Traumatic brain injury is a complex condition consisting of a mechanical injury with neurovascular disruption and inflammation with limited clinical interventions available. A growing number of studies report systemic delivery of human umbilical cord blood (HUCB) as a therapy for neural injuries. Materials & methods: HUCB cells from five donors were tested to improve blood-brain barrier integrity in a traumatic brain injury rat model at a dose of 2.5 × 107 cells/kg at 24 or 72 h postinjury and for immunomodulatory activity in vitro ...
May 10, 2019: Regenerative Medicine
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