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Regenerative Medicine | Page 2

Jonathan Stem, John C Flickinger, Dante Merlino, Ellen M Caparosa, Adam E Snook, Scott A Waldman
Gastrointestinal cancers remain a tremendous burden on society. Despite advances in therapy options, including chemotherapy and radiation, cancer mortality from recurrences and metastases occur frequently. Cancer stem cells (CSCs) drive disease recurrence and metastasis, as these cells are uniquely equipped to self-renew and evade therapy. Therefore, cancer eradication requires treatment strategies that target CSCs in addition to differentiated cancer cells. This review highlights current literature on therapies targeting CSCs in gastrointestinal cancer...
April 26, 2019: Regenerative Medicine
John T Paige, Michael Kremer, Jace Landry, Samuel A Hatfield, Donald Wathieu, Aaron Brug, Daniel J Lightell, Kara L Spiller, T Cooper Woods
Aim: To determine if porcine urinary bladder matrix (UBM) treatment is associated with modulation of wound inflammation in diabetic patients. Patients & methods: mRNA associated with M1 and M2 macrophages were measured in wounds of diabetic and nondiabetic patients pre- and post-treatment with UBM and an M1:M2 score was calculated. Results: Wound tissue from diabetic subjects exhibited elevated M1:M2 scores compared with nondiabetic patients, suggesting a greater pro-inflammatory state prior to treatment...
April 25, 2019: Regenerative Medicine
Dusko Ilic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in February 2019.
April 16, 2019: Regenerative Medicine
Agnieszka Borkowska-Kuczkowska, Dominika Sługocka, Beata Świątkowska-Flis, Dariusz Boruczkowski
Some ocular diseases, such as dystrophies, retinal and macular degeneration, optic nerve atrophy, and Stargardt disease, are progressive and irreversible. In this review, we focus on the use of mesenchymal stem cells (MSCs) in the treatment of these diseases. In animal studies, MSC transplantation significantly delayed retinal degeneration, led to the regeneration of cone cells, and supported the survival of retinal ganglion cells and axon regeneration. In clinical practice, patients with Behcet's disease with retinal vasculitis who received MSC injections experienced a decrease in retinal vasculitis but no improvement in vision acuity...
April 12, 2019: Regenerative Medicine
Dusko Ilic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in January 2019.
April 2, 2019: Regenerative Medicine
Oren Ben-Yosef, Jorge S Burns, Justin A Colacino, Daniel J Kota, Bruno Péault
No abstract text is available yet for this article.
March 19, 2019: Regenerative Medicine
Joan Oliva, Arjie Florentino, Fawzia Bardag-Gorce, Yutaka Niihara
AIM: The study goals were to engineer and harvest scaffold-free undifferentiated/differentiated multilayer human adipose-derived stem cell (hADSC) cell sheets, in absence of treatment. MATERIALS & METHODS:  The hADSC are seeded in 35 mm culture dishes. At confluence or when multilayer cell sheets are formed, hADSC are treated with predefined differentiation culture media (adipocyte, chondrocyte and osteoblast). RESULTS: Undifferentiated hADSC and differentiated adipocyte, osteoblast and chondrocyte hADSC multilayer cell sheets (hADSCmCS) have been harvested...
March 4, 2019: Regenerative Medicine
Shimeng Ji, Chaomin Wu, Lin Tong, Linlin Wang, Jian Zhou, Cuicui Chen, Yuanlin Song
Aim: To determine the efficiency of mesenchymal stem cells (MSCs) of different sources on airway epithelial cells regeneration and track where and to what extent transplanted MSCs home to injured tissues. Materials & methods: We performed DiO-labeled human bone marrow-derived MSCs (hBMSCs) or human chorionic villi-derived MSCs transplantation studies using naphthalene-induced airway injury animal models. Results: Compared with human chorionic villi-derived MSCs, hBMSCs facilitated airway epithelium regeneration faster and better from day 5 after transplantation; moreover, more transplanted hBMSCs distributed in injured lung tissues at the early stage of postinjury, which was mediated by C-X-C motif chemokine ligand 12...
March 2019: Regenerative Medicine
Glyn N Stacey, Peter W Andrews, Ivana Barbaric, Charlotta Boiers, Amit Chandra, Giulio Cossu, Lynn Csontos, Thomas Jr Frith, Jason A Halliwell, Zoe Hewitt, Mark McCall, Harry D Moore, Malin Parmar, M Beatrice Panico, Venkat Pisupati, Valentin P Shichkin, Alison R Stacey, Francesco S Tedesco, Oliver Thompson, Ravenska Wagey
Human stem cells have the potential to transform medicine. However, hurdles remain to ensure that manufacturing processes produce safe and effective products. A thorough understanding of the biological processes occurring during manufacture is fundamental to assuring these qualities and thus, their acceptability to regulators and clinicians. Leaders in both human pluripotent and somatic stem cells, were brought together with experts in clinical translation, biomanufacturing and regulation, to discuss key issues in assuring appropriate manufacturing conditions for delivery of effective and safe products from these cell types...
March 2019: Regenerative Medicine
Li Chen, Zheng-Cai Wang, Jing-Jing Ma, Wen-Jia Sun, Shao-Wen Wang, Zi-Chun Gu, Xuan Yang
AIM: This study explored the effects of local transplantation of autologous nanofat in the treatment of rats with diabetic foot wounds. MATERIALS & METHODS: Nanofat was transplanted into the left foot wound of diabetic rats. Phosphate-buffered saline injection in the right served as control. We measured wound size, the extent of epithelization, microvessel density and the expression levels of cytokines at six different time-points postoperation. RESULTS: Compared with the control feet, nanofat-treated feet had significantly smaller wound areas at 7 and 9 days after grafting and showed better re-epithelialization, a greater number of microvessels and higher levels of angiogenic factor expression...
February 27, 2019: Regenerative Medicine
Serkan Dikici, Naşide Mangır, Frederik Claeyssens, Muhammad Yar, Sheila MacNeil
AIM: In this study, we explored the angiogenic potential and proangiogenic concentration ranges of 2-deoxy-D-ribose (2dDR) and 17β-Estradiol (E2) in comparison with VEGF. The 2dDR and E2 were then loaded into tissue engineering (TE) scaffolds to investigate their proangiogenic potential when released from fibers. MATERIALS & METHODS: Ex ovo chick chorioallantoic membrane (CAM) assay was used to evaluate angiogenic activity of 2dDR and E2. Both factors were then introduced into scaffolds via electrospinning to assess their angiogenic potential when released from fibers...
February 22, 2019: Regenerative Medicine
Eduardo Anitua, Mar Zalduendo, María Troya
AIM: This study investigated the use of the autologous technology of plasma rich in growth factors (PRGF) as a human-based substitute to fetal bovine serum (FBS) in the culture of human dental pulp stem cells. MATERIALS & METHODS: Stem cell characterization was performed. Analysis of isolation, proliferation, migration, trilineage differentiation, senescence and cryopreservation were compared between FBS and PRGF. RESULTS: Human dental pulp stem cell cultures isolated and maintained with PRGF showed a significantly higher number of cells per explant than FBS cultures...
February 15, 2019: Regenerative Medicine
Hendrik Naujokat, Maximilian Lipp, Yahya Açil, Henning Wieker, Falk Birkenfeld, Andre Sengebusch, Florian Böhrnsen, Jörg Wiltfang
AIM: Reconstruction of bone defects with autologous grafts has certain disadvantages. The aim of this study is to introduce a new type of living bioreactor for engineering of bone flaps and to evaluate the effect of different barrier membranes. MATERIALS & METHODS: Scaffolds loaded with bone morphogenetic proteins and bone marrow aspirate wrapped with either a collagen membrane or a periosteal flap were implanted in the greater omentum of miniature pigs. RESULTS: Both histological and radiographic evaluation showed proven bone formation and increased density after 8 and 16 weeks, with an enhanced effect of the periosteal transplant...
February 15, 2019: Regenerative Medicine
Julien Freitag, Dan Bates, James Wickham, Kiran Shah, Leesa Huguenin, Abi Tenen, Kade Paterson, Richard Boyd
AIM: To evaluate the efficacy of autologous adipose-derived mesenchymal stem cell (ADMSC) therapy on pain, function and disease modification in knee osteoarthritis. METHODS: 30 participants with symptomatic knee osteoarthritis were randomized into three groups. Two treatment groups received intra-articular ADMSC therapy consisting of either a single injection (100 × 106 ADMSCs) or two injections (100 × 106 ADMSCs at baseline and 6 months). The third group served as control and continued conservative management...
February 14, 2019: Regenerative Medicine
Sharada Paudel, Wen-Han Lee, Moses Lee, Talal Zahoor, Reed Mitchell, Shang-You Yang, Haiqing Zhao, Lew Schon, Zijun Zhang
AIM: This study investigated a coordinated strategy of revitalizing bone allograft with circulating multipotent stromal cells (MSCs). MATERIALS & METHODS: After chemotactic and releasing assessments, stromal cell-derived factor 1 and platelet-derived growth factor BB in copolymers were coated on the bone allograft (AlloS-P ). Allograft coated with copolymers alone (Allo), as controls, or AlloS-P was implanted into the femur of athymic mice, which received intravenous injections of human MSCs or saline at weeks 1, 2 and 3...
February 14, 2019: Regenerative Medicine
Reza Khorramirouz, Seyedeh M Kameli, Kiarad Fendereski, Seyedeh S Daryabari, Abdol-Mohammad Kajbafzadeh
AIM: The aim of this study was to evaluate the efficacy of tissue-engineered amniotic membrane (AM) in the treatment of myocardial infarction lesions. MATERIALS & METHODS: 20 rats were subjected to coronary arterial ligation in order to induce myocardial infarction injury. Decellularized human AMs were seeded with 2 × 105 adipose-derived mesenchymal stem cells and were implanted in the infarcted hearts. RESULTS & CONCLUSION: Histological and immunohistochemical evaluations indicated the regeneration of cardiomyocytes and reduction of inflammation and fibrosis in the patch-implanted group compared with a control group, 14 days after the surgery...
February 11, 2019: Regenerative Medicine
Dana Alhattab, Fatima Jamali, Dema Ali, Hana Hammad, Sofia Adwan, Reem Rahmeh, Omar Samarah, Bareqa Salah, Mohammad Hamdan, Abdalla Awidi
AIM: Variations in the clinical outcomes using mesenchymal stem cells (MSCs) treatments exist, reflecting different origins and niches. To date, there is no consensus on the best source of MSCs most suitable to treat a specific disease. METHODS: Total transcriptome analysis of human MSCs was performed. MSCs were isolated from two adult sources bone marrow, adipose tissue and two perinatal sources umbilical cord and placenta. RESULTS: Each MSCs type possessed a unique expression pattern that reflects an advantage in terms of their potential therapeutic use...
January 31, 2019: Regenerative Medicine
Shane A Shapiro, Cambray G Smith, Jennifer R Arthurs, Zubin Master
No abstract text is available yet for this article.
January 17, 2019: Regenerative Medicine
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