Pingwei Wang, Dongge Liu, Jiajing Cui, Shuqi Yan, Yujun Liang, Qianqian Chen, Yanping Liu, Shuping Ren, Peng Chen
UNLABELLED: Objective Ultraviolet-B (UVB) radiation is an important factor in causing skin damage. The study is to explore whether 1,25-Dihydroxvitamin D3(1,25(OH)2 D3 ) will attenuate the damage of human immortalized keratinocytes (HaCaT) cells caused by UVB and relevant underlying mechanisms. METHODS: CCK-8 was employed to determine the UVB irradiation intensity and 1,25(OH)2 D3 concentration. Western blot was used to detect the expression of NF-κB, Caspase9, Caspase3, Bax, Bcl2, FADD, CytC, Beclin-1; Flowcytometry was applied to measure the production of ROS...
May 2, 2023: Cutaneous and Ocular Toxicology