Sol-Ah Park, Yoonji Lee, Hyunjoo Hwang, Jeong Hwan Lee, Yang Joo Kang, Yerin Kim, Caiquan Jin, Hyun Joo An, Yoo Jin Oh, Peter Hinterdorfer, Eunhye Kim, Sun Choi, Kisung Ko
Production of therapeutic monoclonal antibody (mAb) in transgenic plants has several advantages such as large-scale production and the absence of pathogenic animal contaminants. However, mAb with high mannose (HM) type glycans has shown a faster clearance compared to antibodies produced in animal cells. The neonatal Fc receptor (FcRn) regulates the persistence of immunoglobulin G (IgG) by the FcRn-mediated recycling pathway, which salvages IgG from lysosomal degradation within cells. In this study, Fc-engineering of antirabies virus therapeutic mAb SO57 with the endoplasmic reticulum (ER)-retention peptide signal (Lys-Asp-Glu-Leu; KDEL) (mAbp K SO57) in plant cell was conducted to enhance its binding activity to human neonatal Fc receptor (hFcRn), consequently improve its serum half-life...
March 2024: Biotechnology Journal