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Biotechnology Journal

Shangshang Sun, Xinlei Wei, Chun You
Laminaribiose is a reducing glucobiose linked by a β-1,3 glycosidic bond; it is also a precursor for building blocks in the pharmaceutical industry, a powerful germinating agent and antiseptic, as well as a potential prebiotic. In this study, an in vitro enzymatic biosystem composed of α-glucan phosphorylase, laminaribiose phosphorylase, isoamylase and 4-glucanotransferase was designed for the one-pot synthesis of laminaribiose from low-cost maltodextrin and glucose. Through condition optimization, 51 mM laminaribiose was produced from 10 g/L maltodextrin (55...
December 13, 2018: Biotechnology Journal
Tjaša Kumelj, Snorre Sulheim, Alexander Wentzel, Eivind Almaas
Streptomyces coelicolor is a model organism for the Actinobacteria, a phylum known to produce an extensive range of different bioactive compounds that include antibiotics currently used in the clinic. Biosynthetic gene clusters discovered in genomes of other Actinobacteria can be transferred to and expressed in S. coelicolor, making it a factory for heterologous production of secondary metabolites. Genome-scale metabolic reconstructions have successfully been used in several biotechnology applications to facilitate the over-production of target metabolites...
December 7, 2018: Biotechnology Journal
Youping Xie, Kongyong Lu, Xurui Zhao, Ruijuan Ma, Jianfeng Chen, Shih-Hsin Ho
Marine microalgae has great potential for lutein production with the advantage of saving fresh water resource. Thus, marine microalga Chlamydomonas sp. JSC4 was investigated as a potential lutein producer in this study. The medium types, nitrate-N and sea salt concentration were individually investigated to promote the cell growth rate and lutein production of JSC4. In Modified Bold Basal 3N medium, cell growth and lutein content were optimal at the nitrate-N concentration of 1000 mg/L and sea salt concentration of 2%...
December 6, 2018: Biotechnology Journal
Sandiswa Mbewana, Ann E Meyers, Edward P Rybicki
Rift valley fever virus (RVFV) is an emerging mosquito borne virus and haemorrhagic fever agent, which causes abortion storms in farmed small ruminants and potentially causes miscarriages in humans. Although live-attenuated vaccines are available for animals, they can only be used in endemic areas and there are currently no commercially available vaccines for humans. Here we describe the production of chimaeric RVFV virus-like particles transiently expressed in Nicotiana benthamiana by Agrobacterium tumefaciens-mediated gene transfer...
November 28, 2018: Biotechnology Journal
Todd Galbraith, Vincent Roy, Jean-Michel Bourget, Tamao Tsutsumi, Maxime Picard-Deland, Jean-François Morin, Robert Gauvin, Ashraf A Ismail, François A Auger, François Gros-Louis
There is a strong clinical need to develop small-caliber tissue-engineered blood vessels for arterial bypass surgeries. Such substitutes can be engineered using the self-assembly approach in which cells produce their own extracellular matrix (ECM), creating a robust vessel without exogenous material. However, this approach is currently limited to the production of flat sheets that need to be further rolled into the final desired tubular shape. In this study, human fibroblasts and smooth muscle cells were seeded directly on UV-C-treated cylindrical polyethylene terephthalate glycol-modified (PETG) mandrels of 4...
November 28, 2018: Biotechnology Journal
Amr S Ali, Ravali Raju, Rashmi Kshirsagar, Alexander R Ivanov, Alan Gilbert, Li Zang, Barry L Karger
There is continual demand to maximize CHO cell culture productivity of a biotherapeutic while maintaining product quality. In this study, a comprehensive multi-omics analysis is performed to investigate the cellular response to the level of dosing of the amino acid cysteine (Cys) in the production of a monoclonal antibody (mAb). When Cys feed levels are insufficient, there is a significant decrease in protein titer. Multi-omics (metabolomics and proteomics, with support from RNAseq) is performed over the time course of the CHO bioprocess producing an IgG1 mAb in 5 L bioreactors...
November 28, 2018: Biotechnology Journal
Zoltán Kis, Robin Shattock, Nilay Shah, Cleo Kontoravdi
To stop the spread of future epidemics and meet infant vaccination demands in low- and middle-income countries, flexible, rapid and low-cost vaccine development and manufacturing technologies are required. Vaccine development platform technologies that can produce a wide range of vaccines are emerging, including: a) humanized, high-yield yeast recombinant protein vaccines; b) insect cell-baculovirus ADDomer vaccines; c) Generalized Modules for Membrane Antigens (GMMA) vaccines; d) RNA vaccines. Herein, existing and future platforms are assessed in terms of addressing challenges of scale, cost, and responsiveness...
November 22, 2018: Biotechnology Journal
Suzanne S Farid
No abstract text is available yet for this article.
February 2019: Biotechnology Journal
(no author information available yet)
No abstract text is available yet for this article.
January 2019: Biotechnology Journal
Jing Zhu, Uta Göbel
No abstract text is available yet for this article.
January 2019: Biotechnology Journal
William E Bentley, Donald K Martin, Takeshi Gotoh
No abstract text is available yet for this article.
December 2018: Biotechnology Journal
Yoon-Mo Koo
No abstract text is available yet for this article.
December 2018: Biotechnology Journal
Mareike Engel, Dirk Holtmann, Roland Ulber, Nils Tippkötter
A future bio-economy should not only be based on renewable raw materials but also in the raise of carbon yields of existing production routes. Microbial electrochemical technologies are gaining increased attention for this purpose. In this study, the electro-fermentative production of biobutanol with C. acetobutylicum without the use of exogenous mediators is investigated regarding the medium composition and the reactor design. It is shown that the use of an optimized synthetic culture medium allows higher product concentrations, increased biofilm formation and higher conductivities compared to a synthetic medium supplemented with yeast extract...
November 20, 2018: Biotechnology Journal
Xin-Yue Lou, Yu-Peng Li, Ying-Wei Yang
Supramolecular nanovalves are an emerging class of important elements that are functionalized on the surfaces of inorganic or hybrid nanocarriers in the constructions of smart cargo delivery systems. Taking advantage of the pseudorotaxane structure via host-guest complexation and the dynamic nature of supramolecular interactions, macrocyclic arene-based supramolecular nanovalves have shown great promise in the applications of drug delivery and controlled release. Careful selection of diverse external stimuli, such as pH variations, temperature changes, redox, enzymes, light irradiation and competitive binding, can activate the opening and closing of the nanovalves by altering the supramolecular structure or binding affinities...
November 20, 2018: Biotechnology Journal
Abir Khalil-Mgharbel, Helena Polena, Paul K Dembélé, Md Mehadi Hasan Sohag, Jean-Pierre Alcaraz, Donald K Martin, Isabelle Vilgrain
BACKGROUND: A major problem for the detection of cancer biomarkers in plasma or serum is that common clinical practice does not require the patient to be in a fasting state. Considering that lipoproteins are the main population affected by food intake, we hypothesized that biomarkers could be embedded in lipid particles and thereby opens a new avenue for detection. AIM: Using our recently published biomarker, soluble VE-cadherin (sVE), we tested our hypothesis using techniques of biophysics, biochemistry and the tools of nanobiotechnology on serum samples from kidney cancer patients (n = 106)...
November 20, 2018: Biotechnology Journal
Rémy Jardillier, Florent Chatelain, Laurent Guyon
With the increased availability of survival datasets, that comprise both molecular information (e.g., gene expression), and clinical information (e.g., patient survival), numerous genes have been proposed as prognostic biomarkers. Despite efforts and money invested, very few of these biomarkers have been clinically validated and are used routinely. A high false discovery rate is assumed to be largely responsible for this, in particular as the number of tested genes is extremely high relative to the number of patients followed...
November 20, 2018: Biotechnology Journal
Yang Wang, Qingtao Liu, Huanjiao Weng, Yanan Shi, Jian Chen, Guocheng Du, Zhen Kang
Promoter is one of the key elements in regulating gene expression. Many natural or synthetic promoters have been modulated by their cis- or tans-regulatory elements to confer instant gene expression change in responding to designated stimuli. In addition, bacterial cells also engage different sigma factors to control the gene expression network at different growth phases or in response to the changing environment and external stresses. In this study, we synthesized a set of promoters that assimilate the endogenous regulation of different sigma factors σ70 , σ38 , σ32 , and σ24 ...
November 20, 2018: Biotechnology Journal
Matheus Regis Belisário-Ferrari, Ren Wei, Tobias Schneider, Annett Honak, Wolfgang Zimmermann
Synthetic plastics such as polyethylene terephthalate (PET) can be cooperatively degraded by microbial polyester hydrolases and carboxylesterases, with the latter hydrolyzing the low-molecular-weight degradation intermediates. For the identification of PET-degrading enzymes, efficient and rapid screening assays are required. Here we report a novel turbidimetric method in a microplate format for the fast screening of enzyme activities against the PET model substrates with two ester bonds bis-(2-Hydroxyethyl) terephthalate (BHET) and ethylene glycol bis-(p-methylbenzoate) (2PET)...
November 15, 2018: Biotechnology Journal
Egon Heuson, Augusto Etchegaray, Stephanie L Filipe, Daniel Beretta, Mickaël Chevalier, Vincent Phalip, François Coutte
Lipopeptides, such as surfactins are important biosurfactants produced by Bacillus sp. that find applications in many areas (environment, medicine, and food industries). Giving their importance, the use of simple detection methods will facilitate screening and quantification. In the present work, the authors describe a completely automated workflow for the screening of lipopeptide-producing strains, including quantification. First, isolated colonies from environmental samples are automatically picked and inoculated in 96 wells growth plate...
November 15, 2018: Biotechnology Journal
Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
The higher intracellular ATP levels of genome-edited strains of P. putida that result from deleting various energy-consuming functions has been exploited for expanding the window of thermal tolerance of this bacterium. Unlike instant growth halt and eventual death of the naturally-occurring strain P. putida KT2440 at 42 °C, the EM42 variant maintained growth and viability of most of the population at the higher temperature for at least 6 h. We took advantage of this quality for implementing a robust thermo-inducible heterologous expression device in this species...
November 12, 2018: Biotechnology Journal
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