Zhuoya Jiao, Yunxia Pan, Fengyuan Chen
Breast cancer is the most common malignant tumor globally as of 2020 and remains the second leading cause of cancer-related death among female individuals worldwide. Metabolic reprogramming is well recognized as a hallmark of malignancy owing to the rewiring of multiple biological processes, notably, glycolysis, oxidative phosphorylation, pentose phosphate pathway, as well as lipid metabolism, which support the demands for the relentless growth of tumor cells and allows distant metastasis of cancer cells. Breast cancer cells are well documented to reprogram their metabolism via mutations or inactivation of intrinsic factors such as c-Myc, TP53, hypoxia-inducible factor, and the PI3K/AKT/mTOR pathway or crosstalk with the surrounding tumor microenvironments, including hypoxia, extracellular acidification and interaction with immune cells, cancer-associated fibroblasts, and adipocytes...
March 29, 2023: Molecular Diagnosis & Therapy
Yanan Wu, Qiwei Hong, Fang Lu, Zhongqiu Zhang, Jingjing Li, Zhenlin Nie, Bangshun He
BACKGROUND: MicroRNA-155 has been discussed as a biomarker in cancer diagnosis and prognosis. Although relevant studies have been published, the role of microRNA-155 remains uncertain because of insufficient data. METHODS: We conducted a literature search in PubMed, Embase, and Web of Science databases to obtain relevant articles and extract data to evaluate the role of microRNA-155 in cancer diagnosis and prognosis. RESULTS: The pooled results showed that microRNA-155 presented a remarkable diagnostic value in cancers (area under the curve = 0...
March 20, 2023: Molecular Diagnosis & Therapy
Awatif M Abuzgaia, Abdelbaset A Elzagallaai, Tara Mullowney, Michael J Rieder
BACKGROUND: Cystic fibrosis (CF) is a genetic disease characterized by multi-system dysfunction resulting in recurrent lung infections and progressive pulmonary disease. CF patients are at a higher risk for drug hypersensitivity reactions (DHRs) compared to the general population, which has been attributed to the recurrent need for antibiotics and the inflammation associated with CF disease. In vitro toxicity tests such as the lymphocyte toxicity assay (LTA) offer the potential for risk assessment for DHRs...
March 20, 2023: Molecular Diagnosis & Therapy
Tobin J Cammett, Katherine Garlo, Ellen E Millman, Kara Rice, Catherine M Toste, Susan J Faas
No abstract text is available yet for this article.
March 4, 2023: Molecular Diagnosis & Therapy
Anju Sharma, Rajnish Kumar, Pritish Varadwaj
Breath analysis is a relatively recent field of research with much promise in scientific and clinical studies. Breath contains endogenously produced volatile organic components (VOCs) resulting from metabolites of ingested precursors, gut and air-passage bacteria, environmental contacts, etc. Numerous recent studies have suggested changes in breath composition during the course of many diseases, and breath analysis may lead to the diagnosis of such diseases. Therefore, it is important to identify the disease-specific variations in the concentration of breath to diagnose the diseases...
February 2, 2023: Molecular Diagnosis & Therapy
Gisèle Nakhlé, Jean-Claude Tardif, Denis Roy, Léna Rivard, Michelle Samuel, Anick Dubois, Jacques LeLorier
RATIONALE: Atrial fibrillation (AF) is associated with an increased risk of thromboembolism. This risk is currently assessed with scoring systems based on clinical characteristics. However, these tools have limited prognostic performance. Circulating biomarkers are proposed for improved prediction of major clinical events and individualization of treatments in patients with AF. OBJECTIVE: The aim was to assess the cost-effectiveness of precision medicine (PM), i...
January 31, 2023: Molecular Diagnosis & Therapy
Yuh Ping Chong, Kay Weng Choy, Christian Doerig, Chiao Xin Lim
The accuracy of diagnostic laboratory tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can impact downstream clinical procedures in managing and controlling the outbreak of coronavirus disease 2019 (COVID-19). To assess the effectiveness of laboratory tools for managing COVID-19 patients in low-income countries (LICs), we systematically searched the PubMed, Embase, Scopus and CINHAL databases for reports published between January 2020 and June 2022. We found that 22 of 1303 articles reported the performance of various SARS-CoV-2 detection tools across 10 LICs...
January 27, 2023: Molecular Diagnosis & Therapy
Ernest J Nelson, Maria A Gubbiotti, Alicia M Carlin, MacLean P Nasrallah, Vivianna M Van Deerlin, Sarah E Herlihy
BACKGROUND AND OBJECTIVE: Determination of isocitrate dehydrogenase (IDH) 1/2 mutational status is crucial for a glioma diagnosis. It is common for IDH mutational status to be determined via a two-step algorithm that utilizes immunohistochemistry studies for IDH1 R132H, the most frequent variant, followed by next-generation sequencing studies for immunohistochemistry-negative or immunohistochemistry-equivocal cases. The objective of this study was to evaluate adding a rapid real-time polymerase chain reaction (RT-PCR) assay to the testing algorithm...
January 23, 2023: Molecular Diagnosis & Therapy
Ruben D Houvast, Maurice van Duijvenvoorde, JiaXin Chua, Mireille Vankemmelbeke, Lindy G Durrant, Akin Inderson, Jeanin E van Hooft, J Sven D Mieog, Bert A Bonsing, Cornelis F M Sier, A Stijn L P Crobach, Alexander L Vahrmeijer, Peter J K Kuppen
BACKGROUND: Targeted molecular imaging may improve tumor cell identification during diagnosis and resection of pancreatic ductal adenocarcinoma (PDAC). Although many molecular imaging biomarkers are (over)expressed in PDAC, intertumoral heterogeneity of biomarker expression hampers universal tracer administration. Preoperative, patient-specific screening and selection of the most optimal biomarker could therefore improve tumor delineation. OBJECTIVE: This study evaluated whether fine-needle biopsy (FNB) specimens could be used to preoperatively predict biomarker expression in the corresponding primary PDAC specimen...
January 19, 2023: Molecular Diagnosis & Therapy
Dhaneshree Bestinee Naidoo, Anil Amichund Chuturgoon
The infectious severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent for coronavirus disease 2019 (COVID-19). Globally, there have been millions of infections and fatalities. Unfortunately, the virus has been persistent and a contributing factor is the emergence of several variants. The urgency to combat COVID-19 led to the identification/development of various diagnosis (polymerase chain reaction and antigen tests) and treatment (repurposed drugs, convalescent plasma, antibodies and vaccines) options...
January 19, 2023: Molecular Diagnosis & Therapy
Francisco J Romeo, Spyros A Mavropoulos, Kiyotake Ishikawa
Despite significant advances in novel treatments and approaches, cardiovascular disease remains the leading cause of death globally. Gene therapy is a promising option for many diseases, including cardiovascular diseases. In the last 30 years, gene therapy has slowly proceeded towards clinical translation and recently reached US Food and Drug Administration approval for several diseases such as Leber congenital amaurosis and spinal muscular atrophy, among others. Previous attempts at developing gene therapies for cardiovascular diseases have yielded promising results in preclinical studies and early-phase clinical trials...
January 15, 2023: Molecular Diagnosis & Therapy
Minas Sakellakis, Athanasios Chalkias
Ion channels have major regulatory functions in living cells. Apart from their role in ion transport, they are responsible for cellular electrogenesis and excitability, and may also regulate tissue homeostasis. Although cancer is not officially classified as a channelopathy, it has been increasingly recognized that ion channel aberrations play an important role in virtually all cancer types. Ion channels can exert pro-tumorigenic activities due to genetic or epigenetic alterations, or as a response to molecular signals, such as growth factors, hormones, etc...
January 4, 2023: Molecular Diagnosis & Therapy
Laura Pala, Vincenzo Bagnardi, Francesca Tettamanzi, Massimo Barberis, Giovanni Mazzarol, Cecilia Casali, Tommaso De Pas, Elisabetta Pennacchioli, Sara Coppola, Federica Baldini, Emilia Cocorocchio, Pierfrancesco Ferrucci, Damiano Patane', Maristella Saponara, Paola Queirolo, Fabio Conforti
BACKGROUND: Data on molecular alterations harbored by melanoma brain metastases (MBMs) are limited, and this has hampered the development of more effective therapeutic strategies. We conducted a systematic review and meta-analysis of all the studies reporting DNA sequencing data of MBMs, in order to identify recurrently mutated genes and molecular pathways significantly enriched for genetic alterations. METHODS: We searched PubMed, Embase and Scopus for articles published from the inception of each database to June 30, 2021...
January 2023: Molecular Diagnosis & Therapy
Xianghui Li, Jing Wang, Jingping Geng, Liming Xiao, Hu Wang
In 2019, a new coronavirus was identified that has caused significant morbidity and mortality worldwide. Like all RNA viruses, severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) evolves over time through random mutation resulting in genetic variations in the population. Although the currently approved coronavirus disease 2019 vaccines can be given to those over 5 years of age and older in most countries, strikingly, the number of people diagnosed positive for SARS-Cov-2 is still increasing...
December 28, 2022: Molecular Diagnosis & Therapy
Hoda Y Abdallah, Ahmed Fareed, Asmaa K K Abdelmaogood, Sahar Allam, Mai Abdelgawad, Loaa A Tag El Deen
BACKGROUND: Atherosclerotic plaque is considered the hallmark of atherosclerotic lesions in coronary atherosclerosis (CAS), the primary pathogenesis in coronary artery disease (CAD), which develops and progresses through a complex interplay between immune cells, vascular cells, and endothelial shear stresses. Early diagnosis of CAS is critical for avoiding plaque rupture and sudden death. Therefore, identifying new CAD biomarkers linked to vessel wall functions, such as RNA molecules with their distinct signature, is a promising development for these patients...
December 20, 2022: Molecular Diagnosis & Therapy
Amir Roointan, Alieh Gholaminejad, Behrokh Shojaie, Kelly L Hudkins, Yousof Gheisari
CONTEXT: Lupus nephritis (LN) is a kidney disease caused by systemic lupus erythematosus in which kidneys are attacked by the immune system. So far, various investigations have reported altered miRNA expression profiles in LN patients and different miRNAs have been introduced as biomarkers and/or therapeutic targets in LN. The aim of this study was to introduce a consensus panel of potential miRNA biomarkers by performing a meta-analysis of miRNA profiles in the LN patients. MATERIALS AND METHODS: A comprehensive literature review approach was performed to find LN-related miRNA expression profiles in renal tissues, blood, and urine samples...
December 15, 2022: Molecular Diagnosis & Therapy
Yura Son, Wuqiang Zhu
Heart disease is the primary cause of death worldwide. Even though extensive research has been done, and many pharmacological and surgical treatments have been introduced to treat heart disease, the mortality rate still remains high. Gene therapy is widely used to understand molecular mechanisms of myocardial infarction and to treat cardiomyocyte loss. It was reported that adult cardiomyocytes proliferate at a very low rate; thus, targeting their proliferation has become a new regenerative therapeutic approach...
December 13, 2022: Molecular Diagnosis & Therapy
(no author information available yet)
No abstract text is available yet for this article.
December 12, 2022: Molecular Diagnosis & Therapy
Aastha Vatsyayan, V R Arvinden, Vinod Scaria
BACKGROUND AND OBJECTIVE: The rapid rate at which the current mpox virus outbreak has spread across the globe has led the World Health Organization to declare it a Public Health Emergency of International Concern. Polymerase chain reaction-based methods are one of the cornerstones for effective molecular detection of viruses including mpox virus. Genetic variants in primer binding sites are known to impact the efficiency of polymerase chain reaction and therefore diagnosis. Here we have analyzed the genetic variants and their impact on efficient binding of oligonucleotides used in diagnostics...
December 10, 2022: Molecular Diagnosis & Therapy
Giang T Lam, Sarita Prabhakaran, Alexandra Sorvina, Carmela Martini, Ben S-Y Ung, Litsa Karageorgos, Shane M Hickey, Joanna Lazniewska, Ian R D Johnson, Desmond B Williams, Sonja Klebe, Victoria Malone, John J O'Leary, Louise Jackett, Doug A Brooks, Jessica M Logan
Cutaneous melanoma is one of the most aggressive forms of skin cancer, with the development of advanced stage disease resulting in a high rate of patient mortality. Accurate diagnosis of melanoma at an early stage is essential to improve patient outcomes, as this enables treatment before the cancer has metastasised. Histopathologic analysis is the current gold standard for melanoma diagnosis, but this can be subjective due to discordance in interpreting the morphological heterogeneity in melanoma and other skin lesions...
December 7, 2022: Molecular Diagnosis & Therapy
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.