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Cancer Biomarkers: Section A of Disease Markers

Shengjun Shi, Binqun Tian
BACKGROUND: Bladder cancer is one of the most common genitourinary malignancies, with a high rate of recurrence and progression. The prognosis for patients with bladder cancer, especially muscle-invasive bladder cancer, remains poor despite systemic therapy. OBJECTIVE: To explore the underlying disease mechanisms and identify more effective biomarkers for bladder cancer. METHODS: Weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) network analysis were applied to identify hub genes correlated with the bladder cancer progression...
January 18, 2019: Cancer Biomarkers: Section A of Disease Markers
Lin Yan, Hai-Hua Yu, Yuan-Shui Liu, Yan-Sen Wang, Wen-Hua Zhao
BACKGROUND: Colorectal cancer (CRC) is the most common malignant disease worldwide and thus new therapeutic approaches are needed. 5-Fluorouracil (5-FU) remains the most widely used agent to treat colorectal cancer (CRC). However, its clinical efficacy is currently limited by the development of drug resistance. Esculetin (EST), a coumarin, was found to have anti-proliferative and anti-migration activity in cancer. OBJECTIVE: This research aims to evaluated the influence and possible mechanism of EST on the proliferation, migration and epithelial-mesenchymal transition of CRC cell lines...
January 18, 2019: Cancer Biomarkers: Section A of Disease Markers
Hang Li, Xiang Pan, Jing Quan, Zuwei Li, Liwen Zhao, Xin Guan, Jinling Xu, Weijie Xu, Yaoting Gui, Yongqing Lai
OBJECTIVE: Renal cell carcinoma (RCC) is one of the most common genitourinary cancers, and advanced RCC usually leads to poor prognosis. Therefore, identifying novel biomarkers for predicting the progression and prognosis of RCC is essential. The present study aims to evaluate the clinical value of miR-183-5p in RCC development and prognosis after surgery. MATERIALS AND METHODS: We enrolled a total of 284 patients who received partial or radical nephrectomy from April 2003 to May 2013 at a single institution...
January 15, 2019: Cancer Biomarkers: Section A of Disease Markers
Juan Wei, Wei Wei, Hanfeng Xu, Zhaojing Wang, Wen Gao, Tianjun Wang, Qin Zheng, Yongqian Shu, Wei De
BACKGROUND: Circular RNAs (circRNA) play key regulatory roles in cancer progression. Human circRNA microarray was performed to screen for abnormally expressed circRNA in gastric cancer tissues. In this study, we found circRNA102958 was up-regulated in gastric cancer tissues and cell lines. METHODS: The hsa_circRNA_102958 levels were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in gastric tissue and cell. Then, the association between the expression level of hsa_circRNA_102958 and the clinicopathological features of patients with gastric cancer was further analyzed...
January 15, 2019: Cancer Biomarkers: Section A of Disease Markers
Heba F Pasha, Randa H Mohamed, Mohamed I Radwan
BACKGROUND: DNA methylation status is one of the most prevalent molecular alterations in human cancers. Identification of powerful diagnostic and prognostic biomarkers for hepatocellular carcinoma (HCC) without a biopsy is urgently required. OBJECTIVE: The purpose of this study was to determine the methylation status of RASSIF1A and SOCS-1genes as a non-invasive biomarker for HCC identification and prognosis. METHODS: Methylation specific-PCR technique was performed to recognize the methylation status of RASSIF1A and SOCS-1 genes in 100 patients with HCC, 100 patients with liver cirrhosis (LC) but without HCC were considered as cirrhotic liver control group and 100 healthy control...
January 14, 2019: Cancer Biomarkers: Section A of Disease Markers
Bing Ji, Youmin Huang, Ting Gu, Li'e Zhang, Guohong Li, Changgeng Zhang
BACKGROUND: Plasma carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and cancer antigen 72-4 (CA72-4) are common markers which are useful in the diagnosis and prognosis of GC. However, their sensitivity and specificity in GC remain unsatisfactory. Identification of cancer diagnosed-biomarkers would be of great value. OBJECTIVE: Evaluate the diagnostic and prognostic value of LINC00086 and miR-214 in GC. METHODS: In this study, we determined the expression of LINC00086 and miR-214 in GC by qRT-PCR...
January 14, 2019: Cancer Biomarkers: Section A of Disease Markers
Hedyeh Ziai, Abdulrahman Alenazi, Matthew Hearn, Daniel A O'Connell, Lakshmi Puttagunta, Brittany Barber, Jeffrey R Harris, Hadi Seikaly, Vincent L Biron
BACKGROUND: The role of molecular biomarkers in oropharyngeal squamous cell carcinoma (OPSCC) has recently been increasingly recognized. There is conflicting evidence in the literature with regards to the prognostic value of p53 and Bcl-xL. OBJECTIVE: The purpose of this study was to investigate the association between p53 and Bcl-xL expression profiles and survival outcomes in OPSCC. METHODS: Patients diagnosed with OPSCC and treated with curative intent between 1998 and 2009 were included in the study...
December 27, 2018: Cancer Biomarkers: Section A of Disease Markers
Jin-Rong Wang, Bin Liu, Lei Zhou, Yue-Xin Huang
BACKGROUND: A growing body of studies has demonstrated the aberrant expression of microRNAs (miRNAs) contributes to human tumor metastasis. MicroRNA-124-3p (miR-124-3p), which is down-regulated in various cancers, has been found to be involved in several signaling pathways relevant to tumor cell migration and invasion. However, the roles of miR-124-3p in human bladder cancer remain unclear. This study aims to investigate the functional significance of miR-124-3p and to understand how it targets the integrin receptor, and thus affects the progression of human bladder cancer...
December 27, 2018: Cancer Biomarkers: Section A of Disease Markers
Mingjing Shen, Lichun Cai, Kanqiu Jiang, Weihua Xu, Yongbin Chen, Zhongheng Xu
Lung cancer represents a major healthy concern due to high incidence and morality. Increasing evidences showed critical regulatory role of microRNA (miR) in cell growth, differentiation and apoptosis. It has been indicated that the level of miR-328 is abnormally up regulated in lung cancer cell line, which is correlated with cell apoptosis. An in vitro lung cancer model was established through induction of chlamydia pneumonia. Western blot and real-time quantitative PCR were used to measure miR-328 level and its effects on histone H2AX expression...
December 27, 2018: Cancer Biomarkers: Section A of Disease Markers
Dongliang Hou, Tong Fang, Linan Song, Baojin Sun, Bo Liu, Li Chen
Oral squamous cell carcinoma (OSCC) is a complex disease with extensive genetic and epigenetic defects, including microRNA deregulation. The aims of the present study were to explore the regulatory mechanism of microRNA-18a in OSCC cells proliferation and metastasis. Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of miR-18a and KLF4 mRNA in OSCC tissues and cell lines (SCC-25, CAL-27, Tca-8113). Western-blot analysis was used to validate expression of KLF4 protein. CCK-8 assay was performed to e validate the proliferous ability of human OSCC cells...
December 26, 2018: Cancer Biomarkers: Section A of Disease Markers
Youfan Feng, Yuxia Zhang, Xiaofang Wei, Qike Zhang
OBJECTIVE: Human multiple myeloma (MM) is a kind of common tumor in middle-aged and elderly people, in which the osteolytic lesion is formed mainly through inhibiting osteoblast (OB) differentiation and promoting osteoclast (OC) differentiation. Dickkopf-1 (DKK1) is a soluble Wnt inhibitor, which has an important correlation with the pathogenesis of human MM. Therefore, the correlations of DKK1 with pathogenesis and prognosis of human MM were investigated in this study. METHODS: The DKK1 expression in tissues and serum of myeloma patients was detected via immunohistochemistry and enzyme-linked immunosorbent assay (ELISA)...
December 26, 2018: Cancer Biomarkers: Section A of Disease Markers
Zhibin Wang, Xuan Zhao, Zhiming Ma, Li Liu, Bin Wang, Yuan Li
Gallbladder carcinoma (GC) occupies more than 90% of all cancers in biliary tract with an increasing incidence. Most patients with GC are already at terminal stage at the time of primary diagnosis, causing unfavorable prognosis and high mortality. Transformation growth factor-beta (TGF-β) is up-regulated in GC. However, the mechanism by how TGF-β is involved in GC remains unclear. The aim of this study was to investigate the effect and mechanism of TGF-β in GC using GC cell line NOZ cells.In vitro cultured NOZ cell was randomly assigned into control, si-NC and TGF-β1 siRNA groups and were transfected with siRNA negative control (NC) or TGF-β1 siRNA followed by analysis of TGF-β1 expression by Real-time PCR, cell proliferation by MTT assay, cell apoptosis and cell invasion, as well as expression of proteins in epithelial-mesenchymal transition (EMT), p38, Smad2/3 and Smad4 phosphorylation by Western blot, Insulin-like growth factor-binding protein-2 (IGFBP-2) level by ELISA...
December 26, 2018: Cancer Biomarkers: Section A of Disease Markers
Longyang Liu, Zhaoyang Zeng, Juanjuan Yi, Liu Zuo, Jin Lv, Jianhuan Yuan, Zhongqiu Lin, Rongcheng Luo, Xin Feng
OBJECTIVES: To investigate TCF4 expression in epithelial ovarian cancer, and to explore its correlation with clinicopathological parameters and clinical prognosis of epithelial ovarian cancer. METHODS: From 2009 to 2017, 188 cases of paraffin-embedded epithelial ovarian cancer tissues and 41 paratumor ovarian tissues which had been confirmed at the memorial hospital of Sun Yat-sen University were collected in this study, and the expression of TCF4 was performed by immunohistochemistry using a polyclonal antibody specific for TCF4...
December 26, 2018: Cancer Biomarkers: Section A of Disease Markers
Hong Li, Huogang Wang, Ke Deng, Wei Han, Bo Hong, Wenchu Lin
BACKGROUND: Cisplatin-based chemotherapy and radiotherapy are the most commonly used treatments for small cell lung cancer (SCLC). However, despise initially dramatic response, the response duration of SCLC patients is variable and resistance to chemo- and radio-therapy inevitably develops. OBJECTIVE: The aim of the study is to investigate the role of Bcl-2 family proteins in predicting SCLC sensitivity to cisplatin treatment, and to identify the potential sensitizer of cisplatin or ratiation treatment in SCLC...
December 25, 2018: Cancer Biomarkers: Section A of Disease Markers
Ha Yoon Mo, Yun Sol Jo, Nam Jin Yoo, Min Sung Kim, Sang Yong Song, Sug Hyung Lee
BACKGROUND: Both QKI and TMEFF2 genes are considered putative tumor suppressor genes (TSGs). In gastric (GC) and colorectal (CRC) cancers, downregulation of their expressions is known to be frequent. However, QKI and TMEFF2 mutations that could potentially inactivate their functions are not reported in cancers. METHODS: In a genome database, we observed that both QKI and TMEFF2 harbor mononucleotide repeats, which could be mutated in cancers with high microsatellite instability (MSI-H)...
December 25, 2018: Cancer Biomarkers: Section A of Disease Markers
Ismael Reyes, Niradiz Reyes, Robert Suriano, Codrin Iacob, Nina Suslina, Anthony Policastro, Augustine Moscatello, Stimson Schantz, Raj K Tiwari, Jan Geliebter
BACKGROUND: Thyroid cancer is the most common endocrine malignancy worldwide, with the predominant form papillary thyroid carcinoma (PTC) representing approximately 80% of cases. OBJECTIVE: This study was addressed to identify potential genes and pathways involved in the pathogenesis of PTC and potential novel biomarkers for this disease. METHODS: Gene expression profiling was carried out by DNA microarray technology. Validation of microarray data by qRT-PCR, western blot, and enzyme linked immunosorbent assay was also performed in a selected set of genes and gene products, with the potential to be used as diagnostic or prognostic biomarkers, such as those associated with cell adhesion, extracellular matrix (ECM) remodeling and immune/inflammatory response...
December 22, 2018: Cancer Biomarkers: Section A of Disease Markers
Ying Yang, Zongduan Zhang, Zhengzheng Wu, Wei Lin, Man Yu
BACKGROUND: Long noncoding RNA MIAT expression is related to the development of some diseases. However, the role of MIAT in melanoma was has seldom been studied. OBJECTIVE: To investigate the effect of the lncRNA MIAT on melanoma cells. METHOD: Microarray was used to analyze the lncRNAs expression in tissue samples. The expression of the lncRNA MIAT was detected by qRT-PCR. A CCK-8 assay was used to assess cell viability, and cell counting was used to analyze cell proliferation...
December 20, 2018: Cancer Biomarkers: Section A of Disease Markers
Zhijian Zhou, Jing Ma
MicroRNAs (miRNAs) have been demonstrated that play a critical role in tumorigenesis. The aim of this study is to identify the functional role of miR-378 in cholangiocarcinoma (CCA). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression levels of miR-378 in human CCA tissue samples and CCA cell lines. The receiver operating characteristic (ROC) curve was established, and the area under the ROC curve (AUC) was calculated to estimate the capacity of miR-378 in distinguishing CCA patients with different TNM stages...
December 19, 2018: Cancer Biomarkers: Section A of Disease Markers
Shiro Wakinoue, Tokuhiro Chano, Tsukuru Amano, Takahiro Isono, Fuminori Kimura, Ryoji Kushima, Takashi Murakami
BACKGROUND: Endometrioid ovarian carcinoma and clear cell ovarian carcinoma are both classified as endometriosis-associated ovarian cancer (EAOC). Despite the high rates of recurrence and mortality of EAOC, no prognostic biomarkers have been determined. ADP-ribosylation factor-like protein 4C (ARL4C) has been reported to be involved in various tumor progression processes, but its clinical significance for predicting prognosis in EAOC cases has never been studied. OBJECTIVE: The present study aimed to determine the clinical significance of ARL4C expression in EAOC prognosis...
December 19, 2018: Cancer Biomarkers: Section A of Disease Markers
Jutta Ries, Christoph Baran, Falk Wehrhan, Manuel Weber, Constantin Motel, Marco Kesting, Emeka Nkenke
BACKGROUND: Altered expressions of miR-186, miR-494 and miR-3651 in whole blood of OSCC patients have already been demonstrated. However, nothing is known about their expression in tumor tissues. This study aimed at evaluating differences in miRNA expression in OSCC tissues compared to healthy oral mucosa and at checking if the altered expression is reflected in corresponding blood. METHODS: In 53 OSCC and 40 healthy mucosal tissues the expression of miR-186, -494 and -3651 was determined by RT-qPCR and expression levels were compared between the groups...
December 18, 2018: Cancer Biomarkers: Section A of Disease Markers
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