Vladimir Holan, Kristina Cechova, Alena Zajicova, Jan Kossl, Barbora Hermankova, Pavla Bohacova, Michaela Hajkova, Magdalena Krulova, Petr Svoboda, Eliska Javorkova
Morphine is an analgesic drug therapeutically administered to relieve pain. However, this drug has numerous side effects, which include impaired healing and regeneration after injuries or tissue damages. It suggests negative effects of morphine on stem cells which are responsible for tissue regeneration. Therefore, we studied the impact of morphine on the properties and functional characteristics of human bone marrow-derived mesenchymal stem cells (MSCs). The presence of μ-, δ- and κ-opioid receptors (OR) in untreated MSCs, and the enhanced expression of OR in MSCs pretreated with proinflammatory cytokines, was demonstrated using immunoblotting and by flow cytometry...
December 2018: Stem Cell Reviews