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Purinergic Signalling

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https://read.qxmd.com/read/30756226/extracellular-atp-and-%C3%AE-nad-alter-electrical-properties-and-cholinergic-effects-in-the-rat-heart-in-age-specific-manner
#1
Ksenia B Pustovit, Viktoria M Potekhina, Alexandra D Ivanova, Alexey M Petrov, Denis V Abramochkin, Vlad S Kuzmin
Extracellular ATP and nicotinamide adenine dinucleotide (β-NAD) demonstrate properties of neurotransmitters and neuromodulators in peripheral and central nervous system. It has been shown previously that ATP and β-NAD affect cardiac functioning in adult mammals. Nevertheless, the modulation of cardiac activity by purine compounds in the early postnatal development is still not elucidated. Also, the potential influence of ATP and β-NAD on cholinergic neurotransmission in the heart has not been investigated previously...
February 12, 2019: Purinergic Signalling
https://read.qxmd.com/read/30684150/evidence-for-detection-of-rat-p2x4-receptor-expressed-on-cells-by-generating-monoclonal-antibodies-recognizing-the-native-structure
#2
Tatsuhiro Igawa, Shuhei Kishikawa, Yoshito Abe, Tomohiro Yamashita, Saki Nagai, Mitsunori Shiroishi, Chinatsu Shinozaki, Hiroyuki Tanaka, Hidetoshi Tozaki-Saitoh, Makoto Tsuda, Kazuhide Inoue, Tadashi Ueda
P2X purinergic receptors are ATP-driven ionic channels expressed as trimers and showing various functions. A subtype, the P2X4 receptor present on microglial cells is highly involved in neuropathic pain. In this study, in order to prepare antibodies recognizing the native structure of rat P2X4 (rP2X4) receptor, we immunized mice with rP2X4's head domain (rHD, Gln111-Val167), which possesses an intact structure stabilized by S-S bond formation (Igawa and Abe et al. FEBS Lett. 2015), as an antigen. We generated five monoclonal antibodies with the ability to recognize the native structure of its head domain, stabilized by S-S bond formation...
January 25, 2019: Purinergic Signalling
https://read.qxmd.com/read/30644036/hydrolysis-of-atp-adp-and-amp-is-increased-in-blood-plasma-of-prostate-cancer-patients
#3
Carla Fernanda Furtado Gardani, Angélica Regina Cappellari, Julia Brandt de Souza, Bruna Tertuliano da Silva, Paula Engroff, Cesar Eduardo Jacintho Moritz, Juliete Nathali Scholl, Ana Maria Oliveira Battastini, Fabrício Figueiró, Fernanda Bueno Morrone
Prostate cancer is among the major malignancies that affect men around the world. Adenine nucleotides are important signaling molecules that mediate innumerous biological functions in pathophysiological conditions, including cancer. These molecules are degraded by several ectoenzymes named ectonucleotidases that produce adenosine in the extracellular medium. Some of these ecto-enzymes can be found in soluble in the blood stream. Thus, the present study aimed to evaluate the hydrolysis of adenine nucleotides (ATP, ADP, and AMP) in the plasma blood of patients with prostate cancer...
January 14, 2019: Purinergic Signalling
https://read.qxmd.com/read/30637575/allergic-sensitization-increases-the-amount-of-extracellular-atp-hydrolyzed-by-guinea-pig-leukocytes
#4
Jaime Chávez, Mario H Vargas, Jesús Martínez-Zúñiga, Ramcés Falfán-Valencia, Enrique Ambrocio-Ortiz, Verónica Carbajal, Rosa Sandoval-Roldán
Increased levels of ATP have been found in the bronchoalveolar lavage of patients with asthma, and subjects with this disease, but not healthy subjects, develop bronchospasm after nebulization with ATP. Because the main mechanism for controlling the noxious effects of extracellular ATP is its enzymatic hydrolysis, we hypothesized that allergic sensitization is accompanied by a decreased functioning of such hydrolysis. In the present study, peripheral blood leukocytes from sensitized and non-sensitized guinea pigs were used for determining the extracellular metabolism (as assessed by inorganic phosphate production) of ATP, ADP, AMP, or adenosine, and for detecting possible changes in the expression (qPCR and Western blot) of major ectonucleotidases (NTPDase1, NTPDase3, and NPP1) and purinoceptors (P2X1 , P2X7 , P2Y4 , and P2Y6 )...
January 13, 2019: Purinergic Signalling
https://read.qxmd.com/read/30612254/purines-2018-basic-and-translational-science-on-purinergic-signaling-and-its-components-for-a-healthy-and-better-world
#5
(no author information available yet)
No abstract text is available yet for this article.
January 5, 2019: Purinergic Signalling
https://read.qxmd.com/read/30604179/integrated-use-of-lc-ms-ms-and-lc-q-tof-ms-targeted-metabolomics-with-automated-label-free-microscopy-for-quantification-of-purine-metabolites-in-cultured-mammalian-cells
#6
S Eric Nybo, Jennifer T Lamberts
Purine metabolites have been implicated as clinically relevant biomarkers of worsening or improving Parkinson's disease (PD) progression. However, the identification of purine molecules as biomarkers in PD has largely been determined using non-targeted metabolomics analysis. The primary goal of this study was to develop an economical targeted metabolomics approach for the routine detection of purine molecules in biological samples. Specifically, this project utilized LC/MS/MS and LC/QTOF/MS to accurately quantify levels of six purine molecules in samples from cultured N2a murine neuroblastoma cells...
January 2, 2019: Purinergic Signalling
https://read.qxmd.com/read/30565027/adenosine-a-2a-and-histamine-h-3-receptors-interact-at-the-camp-pka-pathway-to-modulate-depolarization-evoked-3-h-gaba-release-from-rat-striato-pallidal-terminals
#7
Guadalupe-Elide Morales-Figueroa, Nayeli Rivera-Ramírez, Raúl González-Pantoja, Juan Escamilla-Sánchez, Ubaldo García-Hernández, Emilio J Galván, José-Antonio Arias-Montaño
We previously reported that the activation of histamine H3 receptors (H3 Rs) selectively counteracts the facilitatory action of adenosine A2A receptors (A2A Rs) on GABA release from rat globus pallidus (GP) isolated nerve terminals (synaptosomes). In this work, we examined the mechanisms likely to underlie this functional interaction. Three possibilities were explored: (a) changes in receptor affinity for agonists induced by physical A2A R/H3 R interaction, (b) opposite actions of A2A Rs and H3 Rs on depolarization-induced Ca2+ entry, and (c) an A2A R/H3 R interaction at the level of adenosine 3',5'-cyclic monophosphate (cAMP) formation...
December 18, 2018: Purinergic Signalling
https://read.qxmd.com/read/30547277/microparticulate-p2x7-and-gsdm-d-mediated-regulation-of-functional-il-1%C3%AE-release
#8
Srabani Mitra, Anasuya Sarkar
The pro-inflammatory cytokine IL-1β is a secreted protein that is cleaved by caspase-1 during inflammasome activation upon recognition of internal and external insults to cells. Purinergic receptor P2X7 has been described to be involved in the release pathway of bioactive mature IL-1β by activated immune cells. Microparticle (MP) shedding has also been recently recognized as a manner of cytokine IL-1β release. However, the understanding of purinergic receptor roles in the MP-mediated IL-1β release process is still rudimentary...
December 13, 2018: Purinergic Signalling
https://read.qxmd.com/read/30535987/resveratrol-mediated-reversal-of-changes-in-purinergic-signaling-and-immune-response-induced-by-toxoplasma-gondii-infection-of-neural-progenitor-cells
#9
Nathieli B Bottari, Micheli M Pillat, Maria R C Schetinger, Karine P Reichert, Vanessa Machado, Charles E Assmann, Henning Ulrich, Anielen Dutra, Vera M Morsch, Taís Vidal, Ivana B M Da Cruz, Cinthia Melazzo, Aleksandro Schafer Da Silva
The effects of Toxoplasma gondii during embryonic development have not been explored despite the predilection of this parasite for neurons and glial cells. Here, we investigated the activation of the purinergic system and proinflammatory responses during congenital infection by T. gondii. Moreover, neuroprotective and neuromodulatory properties of resveratrol (RSV), a polyphenolic natural compound, were studied in infected neuronal progenitor cells (NPCs). For this study, NPCs were isolated from the telencephalon of infected mouse embryos and subjected to neurosphere culture in the presence of EGF and FGF2...
December 8, 2018: Purinergic Signalling
https://read.qxmd.com/read/30535950/adenosine-plasma-level-in-patients-with-paroxysmal-or-persistent-atrial-fibrillation-and-normal-heart-during-ablation-procedure-and-or-cardioversion
#10
Baptiste Maille, Marion Marlinge, Donato Vairo, Giovanna Mottola, Linda Koutbi, Pierre Deharo, Marguerite Gastaldi, Marine Gaudry, Claire Guiol, Sara Bottone, Patrick Mace, Rosita Gueant, Mohamed Chefrour, Elsa Martinez, Pierre Michelet, Jean Louis Gueant, Alain Boussuges, Jean Ruf, Emmanuel Fenouillet, Jean Claude Deharo, Régis Guieu, Frederic Franceschi
The mechanism of atrial fibrillation (AF) in patients with normal heart remains unclear. While exogenous adenosine can trigger AF, nothing is known about the behavior of endogenous adenosine plasma level (APL) at the onset of AF and during ablation procedure. Ninety-one patients (68 with paroxysmal AF: 40 males, 66 ± 16 years; 23 with persistent AF: 14 males, 69 ± 11 years) and 18 controls were included. Among paroxysmal patients: i) medical therapy alone was performed in 45 cases and ablation procedure in 23...
December 7, 2018: Purinergic Signalling
https://read.qxmd.com/read/30519859/announcement-of-the-resignation-of-holger-eltzschig-as-an-associate-editor
#11
EDITORIAL
Geoffrey Burnstock
No abstract text is available yet for this article.
December 5, 2018: Purinergic Signalling
https://read.qxmd.com/read/30511252/impact-of-genetic-variations-in-adora2a-gene-on-depression-and-symptoms-a-cross-sectional-population-based-study
#12
Sílvia Oliveira, Ana Paula Ardais, Clarissa Ribeiro Bastos, Marta Gazal, Karen Jansen, Luciano de Mattos Souza, Ricardo Azevedo da Silva, Manuella Pinto Kaster, Diogo Rizzato Lara, Gabriele Ghisleni
Genetic variants involved in adenosine metabolism and its receptors were associated with increased risk for psychiatric disorders, including anxiety, depression, and schizophrenia. Here, we examined an association between a single nucleotide polymorphism in A2A receptor gene (ADORA2A, rs2298383 SNP) with current depressive episode and symptom profile. A total of 1253 individuals from a cross-sectional population-based study were analyzed by the Mini International Neuropsychiatric Interview 5.0. Our data showed that the TT genotype of ADORA2A rs2298383 SNP was associated with reduced risk for depression when compared to the CC/CT genotypes (p = 0...
December 3, 2018: Purinergic Signalling
https://read.qxmd.com/read/30430356/purinergic-implication-in-amyotrophic-lateral-sclerosis-from-pathological-mechanisms-to-therapeutic-perspectives
#13
REVIEW
M Cieślak, K Roszek, M Wujak
Amyotrophic lateral sclerosis (ALS) is a clinically heterogeneous disorder characterized by degeneration of upper motor neurons in the brainstem and lower motor neurons in the spinal cord. Multiple mechanisms of motor neuron injury have been implicated, including more than 20 different genetic factors. The pathogenesis of ALS consists of two stages: an early neuroprotective stage and a later neurotoxic. During early phases of disease progression, the immune system through glial and T cell activities provides anti-inflammatory factors that sustain motor neuron viability...
November 14, 2018: Purinergic Signalling
https://read.qxmd.com/read/30417216/characterization-of-purinergic-receptor-expression-in-arpkd-cystic-epithelia
#14
Oleg Palygin, Daria V Ilatovskaya, Vladislav Levchenko, Christine A Klemens, Lashodya Dissanayake, Anna Marie Williams, Tengis S Pavlov, Alexander Staruschenko
Polycystic kidney diseases (PKDs) are a group of inherited nephropathies marked by formation of fluid-filled cysts along the nephron. Growing evidence suggests that in the kidney formation of cysts and alteration of cystic electrolyte transport are associated with purinergic signaling. PCK/CrljCrl-Pkhd1pck/CRL (PCK) rat, an established model of autosomal recessive polycystic kidney disease (ARPKD), was used here to test this hypothesis. Cystic fluid of PCK rats and their cortical tissues exhibited significantly higher levels of ATP compared to Sprague Dawley rat kidney cortical interstitium as assessed by highly sensitive ATP enzymatic biosensors...
November 11, 2018: Purinergic Signalling
https://read.qxmd.com/read/30415294/hypoxic-expression-of-nlrp3-and-vegf-in-cultured-retinal-pigment-epithelial-cells-contribution-of-p2y-2-receptor-signaling
#15
Fabian Doktor, Philipp Prager, Peter Wiedemann, Leon Kohen, Andreas Bringmann, Margrit Hollborn
Retinal hypoxia is a major condition of the chronic inflammatory disease age-related macular degeneration. Extracellular ATP is a danger signal which is known to activate the NLRP3 inflammasome in various cell systems. We investigated in cultured human retinal pigment epithelial (RPE) cells whether hypoxia alters the expression of inflammasome-associated genes and whether purinergic receptor signaling contributes to the hypoxic expression of key inflammatory (NLRP3) and angiogenic factor (VEGF) genes. Hypoxia and chemical hypoxia were induced by a 0...
November 10, 2018: Purinergic Signalling
https://read.qxmd.com/read/30392016/multiple-steps-determine-cd73-shedding-from-rpe-lipid-raft-localization-ara1-interaction-and-mmp-9-up-regulation
#16
Wei Zhang, Shumin Zhou, Guoping Liu, Fanqiang Kong, Song Chen, Hua Yan
Physiologically, retinal pigment epithelium (RPE) expresses high levels of CD73 in their membrane, converting AMP to immune suppressive adenosine, mediates an anti-inflammatory effect. However, after being exposed to inflammatory factors, RPE rapidly becomes CD73-negative cells, which render RPE's immune suppressive function and accelerate local inflammation. Here, we investigated the mechanism leading to the loss of membrane CD73 in RPE. We found the controversy that when membrane CD73 was significantly diminished in inflammatory RPE, Cd73 mRNA levels were not changed at all...
November 3, 2018: Purinergic Signalling
https://read.qxmd.com/read/30387030/p2x4r-promotes-airway-remodeling-by-acting-on-the-phenotype-switching-of-bronchial-smooth-muscle-cells-in-rats
#17
Li Wang, Xiaoqian Feng, Bing Hu, Qingqing Xia, Xiuqin Ni, Yinli Song
The P2X4 receptor (P2X4R) contributes to airway inflammation and airway remodeling in mice with allergic asthma. However, the molecular mechanism by which P2X4R affects the airway remodeling in allergic asthma remains largely unknown. We established an allergic asthma model by ovalbumin (OVA) inhalation in BALB/c mice. Compared with the mice in the control group, the expression of proliferating cell nuclear antigen (PCNA) increased and that of alpha-smooth muscle actin (α-SMA) decreased in the OVA-challenged mice...
November 1, 2018: Purinergic Signalling
https://read.qxmd.com/read/30362044/expanding-the-clinical-relevance-of-the-5-nucleotidase-cn-ii-nt5c2
#18
REVIEW
Lars Petter Jordheim
Purine metabolism is depending on a large amount of enzymes to ensure cellular homeostasis. Among these enzymes, we have been interested in the 5'-nucleotidase cN-II and its role in cancer biology and in response of cancer cells to treatments. This protein has been cited and studied in a large number of papers published during the last decade for its involvement in non-cancerous pathologies such as hereditary spastic paraplegia, schizophrenia, and blood pressure regulation. Here, we review these articles in order to give an overview of the recently discovered clinical relevance of cN-II...
October 25, 2018: Purinergic Signalling
https://read.qxmd.com/read/30362043/mechanism-of-p2x7-receptor-dependent-enhancement-of-neuromuscular-transmission-in-pannexin-1-knockout-mice
#19
Anna S Miteva, Alexander E Gaydukov, Valery I Shestopalov, Olga P Balezina
P2X7 receptors are present in presynaptic membranes of motor synapses, but their regulatory role in modulation of neurotransmitter release remains poorly understood. P2X7 receptors may interact with pannexin 1 channels to form a purinergic signaling unit. The potential mechanism of P2X7 receptor-dependent modulation of acetylcholine (ACh) release was investigated by recording miniature endplate potentials (MEPPs) and evoked endplate potentials (EPPs) in neuromuscular junctions of wild-type (WT) and pannexin 1 knockout (Panx1-/- ) mice...
October 25, 2018: Purinergic Signalling
https://read.qxmd.com/read/30362042/role-of-purinergic-receptors-in-the-alzheimer-s-disease
#20
REVIEW
Marek Cieślak, Andrzej Wojtczak
Etiology of the Alzheimer's disease (AD) is not fully understood. Different pathological processes are considered, such as amyloid deposition, tau protein phosphorylation, oxidative stress (OS), metal ion disregulation, or chronic neuroinflammation. Purinergic signaling is involved in all these processes, suggesting the importance of nucleotide receptors (P2X and P2Y) and adenosine receptors (A1, A2A, A2B, A3) present on the CNS cells. Ecto-purines, ecto-pyrimidines, and enzymes participating in their metabolism are present in the inter-cellular spaces...
October 25, 2018: Purinergic Signalling
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