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Cytometry. Part A: the Journal of the International Society for Analytical Cytology

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https://read.qxmd.com/read/30729668/nuclear-genome-size-in-contrast-to-sex-chromosome-number-variability-in-the-human-bed-bug-cimex-lectularius-heteroptera-cimicidae
#1
David Sadílek, Tomáš Urfus, Jitka Vilímová, Jiří Hadrava, Jan Suda
The human bed bug Cimex lectularius is one of the most prevalent human ectoparasites in temperate climate zones. The cytogenetic features of this resilient pest include holokinetic chromosomes, special chromosome behavior in meiosis, and numerical variation of chromosomes, where the diploid number ranges from 26 + X1 X2 Y to 26 + X1-20 Y. It is desirable to assess the nuclear DNA content of various cytotypes for a further detailed study of the C. lectularius genome. Detailed knowledge of the DNA content of this parasite could also clarify the origin of additional chromosomes...
February 6, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30729665/high-throughput-automated-single-cell-imaging-analysis-reveals-dynamics-of-glioblastoma-stem-cell-population-during-state-transition
#2
Anastasia P Chumakova, Masahiro Hitomi, Erik P Sulman, Justin D Lathia
Cancer stem cells (CSCs) are a heterogeneous and dynamic self-renewing population that stands at the top of tumor cellular hierarchy and contribute to tumor recurrence and therapeutic resistance. As methods of CSC isolation and functional interrogation advance, there is a need for a reliable and accessible quantitative approach to assess heterogeneity and state transition dynamics in CSCs. We developed a high-throughput automated single cell imaging analysis (HASCIA) approach for the quantitative assessment of protein expression with single-cell resolution and applied the method to investigate spatiotemporal factors that influence CSC state transition using glioblastoma (GBM) CSCs (GSCs) as a model system...
February 6, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30725511/srl-communi-ty-cate
#3
EDITORIAL
Attila Tárnok
No abstract text is available yet for this article.
February 6, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30714682/comprehensive-phenotyping-of-t-cells-using-flow-cytometry
#4
REVIEW
Charlotte M Mousset, Willemijn Hobo, Rob Woestenenk, Frank Preijers, Harry Dolstra, Anniek B van der Waart
The T cell compartment can form a powerful defense against extrinsic (e.g., pathogens) and intrinsic danger (e.g., malignant cells). At the same time, specific subsets of T cells control this process to keep the immune system in check and prevent autoimmunity. A wide variety in T cell functionalities exists, which is dependent on the differentiation and maturation state of the T cells. In this review, we report an overview for the identification of CD4+ T-αβ cells (T-helper (Th)1, Th2, Th9, Th17, Th22, and CD4+ regulatory T cells), CD8+ T-αβ cells (cytotoxic T lymphocyte (Tc)1, Tc2, Tc9, Tc17, and CD8+ regulatory T cells), and their additional effector memory status (naïve, stem cell memory, central memory, effector memory, and effector) using flow cytometry...
February 4, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30714674/robust-cell-detection-and-segmentation-for-image-cytometry-reveal-th17-cell-heterogeneity
#5
Takahiro Tsujikawa, Guillaume Thibault, Vahid Azimi, Sam Sivagnanam, Grace Banik, Casey Means, Rie Kawashima, Daniel R Clayburgh, Joe W Gray, Lisa M Coussens, Young Hwan Chang
Image cytometry enables quantitative cell characterization with preserved tissue architecture; thus, it has been highlighted in the advancement of multiplex immunohistochemistry (IHC) and digital image analysis in the context of immune-based biomarker monitoring associated with cancer immunotherapy. However, one of the challenges in the current image cytometry methodology is a technical limitation in the segmentation of nuclei and cellular components particularly in heterogeneously stained cancer tissue images...
February 4, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30701669/broad-immune-phenotyping-of-innate-and-adaptive-leukocytes-in-the-brain-spleen-and-bone-marrow-of-an-orthotopic-murine-glioblastoma-model-by-mass-cytometry
#6
Sophie A Dusoswa, Jan Verhoeff, Juan J Garcia-Vallejo
No abstract text is available yet for this article.
January 31, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30688025/optimization-of-receptor-occupancy-assays-in-mass-cytometry-standardization-across-channels-with-qsc-beads
#7
Gerd Haga Bringeland, Lucius Bader, Nello Blaser, Lisa Budzinski, Axel R Schulz, Henrik E Mei, Kjell-Morten Myhr, Christian A Vedeler, Sonia Gavasso
Receptor occupancy, the ratio between amount of drug bound and amount of total receptor on single cells, is a biomarker for treatment response to therapeutic monoclonal antibodies. Receptor occupancy is traditionally measured by flow cytometry. However, spectral overlap in flow cytometry limits the number of markers that can be measured simultaneously. This restricts receptor occupancy assays to the analysis of major cell types, although rare cell populations are of potential therapeutic relevance. We therefore developed a receptor occupancy assay suitable for mass cytometry...
January 27, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30681265/omip-xxx-an-18-color-panel-for-measuring-th1-th2-th17-and-tfh-responses-in-rhesus-macaques
#8
Mitzi M Donaldson, Shing-Fen Kao, Kathryn E Foulds
No abstract text is available yet for this article.
January 25, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30663857/cytometry-score-23-to-4
#9
EDITORIAL
Vera S Donnenberg, Susann Müller, Jessica P Houston, Elena Holden
No abstract text is available yet for this article.
January 21, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30604477/single-cell-redox-states-analyzed-by-fluorescence-lifetime-metrics-and-tryptophan-fret-interaction-with-nad-p-h
#10
Ruofan Cao, Horst Wallrabe, Karsten Siller, Shagufta Rehman Alam, Ammasi Periasamy
Redox changes in live HeLa cervical cancer cells after doxorubicin treatment can either be analyzed by a novel fluorescence lifetime microscopy (FLIM)-based redox ratio NAD(P)H-a2%/FAD-a1%, called fluorescence lifetime redox ratio or one of its components (NAD(P)H-a2%), which is actually driving that ratio and offering a simpler and alternative metric and are both compared. Auto-fluorescent NAD(P)H, FAD lifetime is acquired by 2- photon excitation and Tryptophan by 3-photon, at 4 time points after treatment up to 60 min demonstrating early drug response to doxorubicin...
January 2, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30584695/omip-0xx-identification-classification-and-isolation-of-major-foxp3-expressing-human-cd4-treg-subsets
#11
Johannes Nowatzky, Cristy Stagnar, Olivier Manches
No abstract text is available yet for this article.
December 24, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30578619/two-color-analysis-of-leukocytes-labeled-by-modified-rbcs-and-their-fragments
#12
Mohamed K Al-Essa, Susanne Melzer, Attila Tárnok
Red blood cells (RBCs) are attractive tools for surface modification to adhere specifically to molecules, cellular fragments (e.g., microvesicles), or whole cells for potential use in bioanalytical assays or as a delivery vehicle in targeted therapy. Within this study, we have loaded RBCs with fluorochrome-conjugated antibodies (Ab) against CD45 and CD22 leukocyte markers and evaluated the conjugation process by microscopy. We have assessed the potential application of RBCs fragments generated from conjugated RBCs for targeting Cyto-Trol control cells by flow cytometric (FCM) approaches...
December 21, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30576071/a-reproducible-objective-method-using-mitotracker%C3%A2-fluorescent-dyes-to-assess-mitochondrial-mass-in-t-cells-by-flow-cytometry
#13
Genevieve Clutton, Katie Mollan, Michael Hudgens, Nilu Goonetilleke
MitoTracker ® dyes are fluorescent compounds that allow cellular mitochondrial content to be measured semi-quantitatively by flow cytometry and have been used extensively in immunology publications. However, the parameters commonly reported, mean or median fluorescence intensity and percentage of cells that are MitoTracker® "high", can be influenced by variability in cytometer setup, dye stability, and operator subjectivity, making it difficult to compare data between experiments. Here, we describe a method to identify MitoTracker® "high" populations in an objective manner...
December 21, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30570217/implementation-and-validation-of-an-automated-flow-cytometry-analysis-pipeline-for-human-immune-profiling
#14
Valerie K Conrad, Christopher J Dubay, Mehrnoush Malek, Ryan R Brinkman, Yoshinobu Koguchi, William L Redmond
Automated reagent preparation, sample processing, and data acquisition have increased the rate at which flow cytometry data can be generated. Furthermore, advances in technology and flow cytometry instrumentation continually increase the complexity and dimensionality of this data. Together, this leads to increased pressure on manual data analysis, which has inherent limitations including subjectivity of the analyst and the length of time needed for data processing. These issues can create bottlenecks in the data processing workflow and potentially compromise data quality...
December 20, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30565841/deep-learning-in-image-cytometry-a-review
#15
REVIEW
Anindya Gupta, Philip J Harrison, Håkan Wieslander, Nicolas Pielawski, Kimmo Kartasalo, Gabriele Partel, Leslie Solorzano, Amit Suveer, Anna H Klemm, Ola Spjuth, Ida-Maria Sintorn, Carolina Wählby
Artificial intelligence, deep convolutional neural networks, and deep learning are all niche terms that are increasingly appearing in scientific presentations as well as in the general media. In this review, we focus on deep learning and how it is applied to microscopy image data of cells and tissue samples. Starting with an analogy to neuroscience, we aim to give the reader an overview of the key concepts of neural networks, and an understanding of how deep learning differs from more classical approaches for extracting information from image data...
December 19, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30565839/simultaneous-purification-of-round-and-elongated-spermatids-from-testis-tissue-using-a-facs-based-dna-ploidy-assay
#16
R B Struijk, C M De Winter-Korver, S K M van Daalen, B Hooibrink, S Repping, A M M van Pelt
Spermiogenesis is the final phase of spermatogenesis during which post-meiotic haploid round spermatids (rSpt) differentiate into elongated spermatozoa and includes several critical cell-specific processes like DNA condensation, formation of the acrosome, and production of the flagellum. Disturbances in this process will lead to complications in sperm development and subsequently cause infertility. As such, studying spermiogenesis has clinical relevance in investigating the etiology of male infertility and will improve our scientific understanding of male germ cell formation...
December 19, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30561906/novel-impactor-and-microsphere-based-assay-used-to-measure-containment-of-aerosols-generated-in-a-flow-cytometer-cell-sorter
#17
Stephen P Perfetto, Phillip J Hogarth, Simon Monard, Ben Fontes, Kristen M Reifel, Brandon K Swan, Jan Baijer, Evan R Jellison, Geoffrey Lyon, Patty Lovelace, Richard Nguyen, David Ambrozak, Kevin L Holmes
Today's state-of-the-art cell sorting flow cytometers are equipped with aerosol containment systems designed to evacuate aerosols from the sort chamber during a sort. This biosafety device is especially important when the sort operator is sorting infectious or potentially infections samples. Hence, it is critical to evaluate the performance for this system in normal operation and in "failure" mode to determine the efficacy of containment. In the past decade, the most popular published method for evaluating containment has been the Glo-Germ bead procedure...
December 18, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30556955/chromosome-analysis-using-benchtop-flow-analysers-and-high-speed-cell-sorters
#18
Bee L Ng, Beiyuan Fu, Jennifer Graham, Christopher Hall, Sam Thompson
The use of the DNA dyes Hoechst (HO) and chromomycin A3 (CA3) has become the preferred combination for the bivariate analysis of chromosomes from both human and animals. This analysis requires a flow cytometer equipped with lasers of specific wavelength and of higher power than is typical on a conventional bench top flow cytometer. In this study, we have investigated the resolution of chromosome peaks in a human cell line with normal flow karyotype using different combinations of DNA dyes on a number of flow cytometers available in a flow cytometry core facility...
December 17, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30556331/quality-assessment-of-ki67-staining-using-cell-line-proliferation-index-and-stain-intensity-features
#19
Mathias Buus Lanng, Cecilie Brochdorff Møller, Anne-Sofie Hendrup Andersen, Ásgerður Arna Pálsdóttir, Rasmus Røge, Lasse Riis Østergaard, Alex Skovsbo Jørgensen
Breast cancer is the most frequent cancer among women worldwide. Ki67 can be used as an immunohistochemical pseudo marker for cell proliferation to determine how aggressive the cancer is and thereby the treatment of the patient. No standard Ki67 staining protocol exists, resulting in inter-laboratory stain variability. Therefore, it is important to determine the quality control of a staining protocol to ensure correct diagnosis and treatment of patients. Currently, quality control is performed by the organization NordiQC that use an expert panel-based qualitative assessment system...
December 17, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://read.qxmd.com/read/30556307/monitoring-circulating-tumor-cells-in-vivo-by-a-confocal-microscopy-system
#20
Yuhao Hu, Wanyi Tang, Pan Cheng, Quanyu Zhou, Xiaoying Tian, Xunbin Wei, Hao He
Circulating tumor cells (CTCs) play a key role in cancer metastasis but are very difficult to detect. in vivo monitoring CTCs has been recognized as an important technique for cancer research and clinical diagnosis. Recently, a noninvasive method, in vivo flow cytometry (IVFC) has been developed to enable continuous, real-time, and long-duration detection of CTCs in animal models by detecting CTC fluorescence in blood vessels excited by lasers. In this study, we present a simple optical scheme for direct noninvasive CTC detection using confocal microscopes...
December 17, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
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