Martijn W C Verbeek, Beatriz Soriano Rodríguez, Lukasz Sedek, Anna Laqua, Chiara Buracchi, Malicorne Buysse, Michaela Reiterová, Elen Oliveira, Daniela Morf, Sjoerd R Oude Alink, Susana Barrena, Saskia Kohlscheen, Stefan Nierkens, Mattias Hofmans, Paula Fernandez, Elaine Sobral de Costa, Ester Mejstrikova, Tomasz Szczepanski, Lukasz Slota, Monika Brüggemann, Giuseppe Gaipa, Georgiana Grigore, Jacques J M van Dongen, Alberto Orfao, Vincent H J van der Velden
Presence of minimal residual disease (MRD), detected by flow cytometry, is an important prognostic biomarker in the management of B-cell precursor acute lymphoblastic leukemia (BCP-ALL). However, data-analysis remains mainly expert-dependent. In this study, we designed and validated an Automated Gating & Identification (AGI) tool for MRD analysis in BCP-ALL patients using the two tubes of the EuroFlow 8-color MRD panel. The accuracy, repeatability, and reproducibility of the AGI tool was validated in a multicenter study using bone marrow follow-up samples from 174 BCP-ALL patients, stained with the EuroFlow BCP-ALL MRD panel...
September 22, 2023: Cytometry. Part B, Clinical Cytometry