Journals International Journal of Immun...

International Journal of Immunogenetics
Patrick A Flynn, Sebastian Fernando, Judith E Worthington, Kay V Poulton
The aim of this study was to devise an algorithm that would predict flow cytometry crossmatch (FCXM) results using single-antigen bead (SAB) mean fluorescent intensity (MFI) levels using samples received through the National External Quality Assurance Scheme (NEQAS) 2B external proficiency testing scheme between 2019 and 2023. A total of 159 serum samples were retrospectively screened using LABScreen Single Antigen Class I and II (SAB), and 40 peripheral blood samples were human leucocyte antigen (HLA) typed with LABType SSO...
February 19, 2024: International Journal of Immunogenetics
Steven G E Marsh
No abstract text is available yet for this article.
February 13, 2024: International Journal of Immunogenetics
Angeles Carrillo, María Jimena Manzur, Maximiliano Juri Ayub
Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies...
January 31, 2024: International Journal of Immunogenetics
Huitao Wu, Xuan Zhang, Guiling Lin, Qi Zhang, Ziman He, Zhe Wang, Wenlu Xu, Xiyu Yin, Linglan Su, Yanping Zhuang, Aimin Gong
To investigate the correlation between susceptibility to systemic lupus erythematosus (SLE) and single nucleotide polymorphisms (SNPs) rs699, rs4762 and rs1926723 in the AGT gene in the population of Northeast China, while also introducing a new method for early detection of SLE. A total of 856 cases of SLE patients and healthy volunteers who attended the First Affiliated Hospital of Harbin Medical University from January 2020 to December 2022 were recruited. Clinical information and biood samples were collected from particpants in this study...
January 24, 2024: International Journal of Immunogenetics
Jeffy J Fernando, Raja Biswas, Lalitha Biswas
Solid organ transplantation is a life-saving intervention for individuals with end-stage organ failure. Despite the effectiveness of immunosuppressive therapy, the risk of graft rejection persists in all viable transplants between individuals. The risk of rejection may vary depending on the degree of compatibility between the donor and recipient for both human leucocyte antigen (HLA) and non-HLA gene-encoded products. Monitoring the status of the allograft is a critical aspect of post-transplant management, with invasive biopsies being the standard of care for detecting rejection...
January 10, 2024: International Journal of Immunogenetics
Ruyu Ren, Huiling Tan, Xuean Wang, Li Wang, Bin Yang
Rheumatoid arthritis (RA) is a chronic inflammatory disease which is closely related to genetic background. Single-nucleotide polymorphisms (SNPs) have been found to play an important role in the development of RA. This study intends to investigate the links between gene polymorphisms in the interleukin-23 receptor (IL23R) and interleukin 17A (IL17A) and susceptibility to RA in the Western Chinese Han population. Four SNPs (rs6693831 T > C, rs1884444 G > T, and rs7517847 T > G in IL23R gene, and rs2275913 G > A in IL17A gene) were genotyped in 246 RA patients and 362 healthy controls by high resolution melting analysis...
January 9, 2024: International Journal of Immunogenetics
Beatrice Valatkaite-Rakstiene, Rita Cekauskiene, Tadas Zvirblis, Arturas Jakubauskas
The patient-donor human leukocyte antigen (HLA) match remains the most important prognostic factor for successful unrelated donor haematopoietic stem cell transplantation (UD-HSCT). This single-centre study comprised 125 adult patients with malignant haematological diseases undergoing their first UD-HSCT. The primary goal of this study was to validate the impact of HLA matching on HSCT outcomes, specifically at the HLA-DPB1 and HLA-DRB3/4/5 loci. A multivariable Cox regression analysis with a backward selection algorithm was employed to assess the associations of selected prognostic factors with outcomes after UD-HSCT...
January 6, 2024: International Journal of Immunogenetics
Deborah Pritchard, Arthi Anand, Amy De'Ath, Helena Lee, Margaret Tracey Rees
Coeliac disease is a common immune-mediated inflammatory disorder caused by dietary gluten in genetically susceptible individuals. While the diagnosis of coeliac disease is based on serological and histological criteria, HLA-DQ genotyping can be useful, especially in excluding the diagnosis in patients who do not carry the relevant DQ heterodimers: DQA1*05 DQB1*02, DQB1*03:02 or DQA1*02 DQB1*02 (commonly referred to as DQ2.5, DQ8 and DQ2.2, respectively). External quality assessment results for HLA genotyping in coeliac disease have revealed concerning errors in HLA genotyping, reporting and clinical interpretation...
December 28, 2023: International Journal of Immunogenetics
Ahmed Alyami, Abdullah AlJurayyan, Bandar Alosaimi, Haitham Alkadi, Fadwa Alkhulaifi, Haya Al-Jurayb, Awad Osman, Steve Christmas, Suliman Alomar, Zaid Al-Bayati
Human leukocyte antigen-G (HLA-G) is classified as non-classical HLA, located in the short arm of chromosome 6 and composed of seven introns and eight exons. The HLA-G gene has a lower frequency polymorphism in the coding area and higher variability at the regulatory 5'- and 3'-untranslated regions linked to HLA-G microRNA regulation. HLA-G molecule is known to have an immunomodulatory and tolerogenic features role. In 199 Saudi individuals, we examined the association between plasma soluble HLA-G (sHLA-G) levels and eight polymorphic different sites, including 14 bp ins/del/+3003T-C/+3010C-G/+3027C-A/+3035C-T/+3142C-G/+3187A-G/+3196C-G single nucleotide polymorphisms (SNPs) in exon 8 in the HLA-G gene...
December 12, 2023: International Journal of Immunogenetics
Wei Tian, Li Xin Li, Wen Cheng, He Kun Jin, Sha Shuang Zhang
Among the thirteen leukocyte Ig-like receptor (LILR) loci located at 19q13.4, LILRA3 is unique in that it encodes a soluble protein lacking the transmembrane and cytoplasmic domains, and a 6.7 kb deletion spanning the first seven exons has been detected in some human individuals. Presently, there is a lack of data about the distribution of LILRA3 gene deletion in more diverse ethnic groups. Also, no previous studies have investigated the correlation between copy number variation (CNV) of LILRA3 and nasopharyngeal carcinoma (NPC)...
November 28, 2023: International Journal of Immunogenetics
Juliana da Silveira Schauren, Amanda Henrique de Oliveira, Camila Rosat Consiglio, Odirlei André Monticielo, Ricardo Machado Xavier, Natália Schneider Nunes, Joel Henrique Ellwanger, José Artur Bogo Chies
This study investigated the impacts of CCR5 promoter region polymorphisms on the development of systemic lupus erythematosus (SLE) by comparing CCR5 genotypes and haplotypes from SLE patients with ethnically matched controls. A total of 382 SLE patients (289 European-derived and 93 African-derived) and 375 controls (243 European-derived and 132 African-derived) were genotyped for the CCR2-64I G > A (rs1799864), CCR5-59353 C > T (rs1799988), CCR5-59356 C > T (rs41469351), CCR5-59402 A > G (rs1800023) and CCR5-59653 C > T (rs1800024) polymorphisms through polymerase chain reaction-restriction fragment length polymorphism and direct sequencing...
November 20, 2023: International Journal of Immunogenetics
Tianchang Lu, Yuhan Shi, Minyi Wang, Weipeng Liu, Yang Cao, Li Shi, Qianli Ma, Shuyuan Liu
C-C chemokine receptor 5 (CCR5) plays a crucial role in the regulation of immune cell activation and migration as well as the progression of many cancers. We performed an in silico analysis using public data resources and found that the lung cancer patients with higher CCR5 expression had a notably better overall survival than those with lower CCR5 expression patients and CCR5 expression level is positive correlated with the infiltration of immune cells, such as B, CD8+ T and CD4+ T cells, in both lung adenocarcinoma and lung squamous cell cancer...
November 14, 2023: International Journal of Immunogenetics
Rita Angélica Pineda-Sic, David Vega-Morales, Leticia Santoyo-Fexas, Mario Alberto Garza-Elizondo, Andrés Mendiola-Jiménez, Karina Itzel González Marquez, Berenice Carrillo-Haro
OBJECTIVE: Rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibody (anti-CCP) are commonly used for diagnosis of rheumatoid arthritis (RA), although other rheumatic diseases with arthritis can test positive. This study aimed to determine the cutoff values for RF and anti-CCP with the best diagnostic performance in a sample of patients with RA, compared with other rheumatic diseases. METHODS: This was a descriptive, prospective study. EUROINMMUN enzyme-linked immunosorbent assays for RF isotypes immunoglobulin (Ig) A (IgA), IgG and IgM and third-generation assay IgG for anti-CCP were used in serum samples of patients with RA, other rheumatic diseases and healthy subjects...
November 6, 2023: International Journal of Immunogenetics
Richard Battle, Deborah Pritchard, Sarah Peacock, Catherine Hastie, Judith Worthington, Sue Jordan, Jennifer A McCaughlan, Martin Barnardo, Rebecca Cope, Claire Collins, Natalia Diaz-Burlinson, Carla Rosser, Luke Foster, Delordson Kallon, Olivia Shaw, David Briggs, David Turner, Arthi Anand, Arash Akbarzad-Yousefi, Deborah Sage
Solid organ transplantation represents the best (and in many cases only) treatment option for patients with end-stage organ failure. The effectiveness and functioning life of these transplants has improved each decade due to surgical and clinical advances, and accurate histocompatibility assessment. Patient exposure to alloantigen from another individual is a common occurrence and takes place through pregnancies, blood transfusions or previous transplantation. Such exposure to alloantigen's can lead to the formation of circulating alloreactive antibodies which can be deleterious to solid organ transplant outcome...
November 2023: International Journal of Immunogenetics
Steven G E Marsh
No abstract text is available yet for this article.
October 26, 2023: International Journal of Immunogenetics
Aslihan Esra Bildirici
Familial Mediterranean fever (FMF) is an inherited disorder caused by the gain of function mutations in MEFV (MEditerranean FeVer) gene loci. FMF affects more than 100,000 people worldwide and generally seen in the eastern Mediterranean region and causes the lifelong diseases which have a significant effect on the patient's life quality and health systems. The identification of low penetrant or heterozygous MEFV gene mutations in clinically diagnosed FMF patients was considered that epigenetic or environmental factors may display a role in FMF pathogenesis...
October 4, 2023: International Journal of Immunogenetics
Quan Yao Ho, Chew Yen Phang, Ian Tatt Liew, May Ling Lai, Carolyn Shan-Yeu Tien, Sobhana Thangaraju, Marieta Chan, Terence Kee
Human leucocyte antigen (HLA) alleles may generate antibodies that are undetectable by routine single-antigen beads (SABs) assays if their unique epitopes are unrepresented. We aimed to describe the prevalence and explore the potential impact of unrepresented HLA alleles in standard SAB kits in our cohort. All individuals who had undergone two-field HLA typing (HLA-A/B/C/DRB1/DQA1/-DQB1/-DPA1/-DPB1) from February 2021 to July 2023 were included. Two-field HLA-DRB3/4/5 typing was imputed. Each unrepresented allele was compared with the most similar represented allele in the standard LABScreen, LABScreen ExPlex (One Lambda) and the LIFECODES (Immucor) SAB kits...
September 30, 2023: International Journal of Immunogenetics
Hai Cheng, Fenglan Sun, Yaoling Ouyang, Chengbin Li
Hepatitis B virus (HBV) is responsible for various liver diseases, such as chronic hepatitis B (CHB), liver fibrosis, liver cirrhosis (LC) and hepatocellular carcinoma (HCC), which pose a significant threat to human health. An ineffective immune response to HBV can result in viral chronicity. Interleukin-37 (IL-37), an immunomodulator, is capable of inhibiting both innate and adaptive immune responses. It is believed that single nucleotide polymorphisms (SNPs) within the IL-37 gene could contribute to the regulation of HBV clearance...
September 21, 2023: International Journal of Immunogenetics
Jiabin Zhan, Rui Li, Yi Ye, Jing Zheng, Gang Wang, Jinli Wu, Xin Wei, Min Zeng
Allergic rhinitis (AR) is a disease that is difficult to cure and accompanies the patient's life. Proinflammatory cytokines (GM-CSF and eotaxin) and MUC5AC are key mediators promoting AR progression. Herein, the function of lncRNA ZFAS1 in AR was investigated. Nasal epithelial cells (NECs) were subjected to 50 ng/mL IL-13 for 24 h to construct an AR cell model. The mRNA and protein expressions were assessed using qRT-PCR and western blot. The levels of GM-CSF, eotaxin, IL-1β, IL-6, TNF-α and MUC5AC in cell supernatant were examined by ELISA...
September 12, 2023: International Journal of Immunogenetics
Jing Xu, Haiyan Chen, Chen Sun, Siyu Wei, Junxian Tao, Zhe Jia, Xingyu Chen, Wenhua Lv, Hongchao Lv, Guoping Tang, Yongshuai Jiang, Mingming Zhang
The aim of this study was to compare nonrandom associations between physically adjacent single methylation polymorphism loci among rheumatoid arthritis (RA) and normal subjects for investigating RA-risk methylation haplotypes (meplotype). With 354 ACPA-positive RA patients and 335 normal controls selected from a case-control study based on Swedish population, we conducted the first RA epigenome-wide meplotype association study using our software EWAS2.0, mainly including (i) converted the β value to methylation genotype (menotype) data, (ii) identified methylation disequilibrium (MD) block, (iii) calculated frequent of each meplotypes in MD block and performed case-control association test and (iv) screened for RA-risk meplotypes by odd ratio (OR) and p-values...
September 9, 2023: International Journal of Immunogenetics
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