Anil Kumar Verma, Arun Goyal, Filipe Freire, Pedro Bule, Immacolata Venditto, Joana L A Brás, Helena Santos, Vânia Cardoso, Cecília Bonifácio, Andrew Thompson, Maria João Romão, José A M Prates, Luís M A Ferreira, Carlos M G A Fontes, Shabir Najmudin
The modular carbohydrate-active enzyme belonging to glycoside hydrolase family 30 (GH30) from Clostridium thermocellum (CtXynGH30) is a cellulosomal protein which plays an important role in plant cell-wall degradation. The full-length CtXynGH30 contains an N-terminal catalytic module (Xyn30A) followed by a family 6 carbohydrate-binding module (CBM6) and a dockerin at the C-terminus. The recombinant protein has a molecular mass of 45 kDa. Preliminary structural characterization was carried out on Xyn30A crystallized in different conditions...
December 2013: Acta Crystallographica. Section F, Structural Biology and Crystallization Communications