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Mark R Cronan, David M Tobin
No abstract text is available yet for this article.
April 10, 2019: Zebrafish
Erick Navarro-Barrón, Crisantema Hernández, Raúl Llera-Herrera, Alejandra García-Gasca, Bruno Gómez-Gil
Diet modulates the gut microbiota and is one of the main factors promoting obesity and overweight. In the present study, we investigated the effect of a high-fat diet (HFD) on the gut microbiota of the zebrafish (Danio rerio). Fish were separated into three groups and fed in different regimes: low fat, high fat, and high fat overfed; the experiments were performed on males and females separately. We analyzed more than 2.6 million sequences of variable region V3 of the 16S rRNA gene generated by the Illumina Miniseq platform, clustered to 97% similarity with vsearch and classified with the EzBioCloud database...
April 9, 2019: Zebrafish
Alexander Neukum, Andreas Bartschat, Helmut Breitwieser, Uwe Strähle, Thomas Dickmeis, Christian Pylatiuk
No abstract text is available yet for this article.
April 2, 2019: Zebrafish
Eda Altan, Steven V Kubiski, Ákos Boros, Gábor Reuter, Mohammadreza Sadeghi, Xutao Deng, Erica K Creighton, Marcus J Crim, Eric Delwart
Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20% in their P1 capsids and <36% in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of Cyprivirus. Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56%) institutions tested...
April 2, 2019: Zebrafish
Daiki Yamamoto, Daisuke Sato, Hiroko Nakayama, Yuki Nakagawa, Yasuhito Shimada
Zebrafish embryos and larvae have become popular vertebrate models because their body walls are transparent, which enables live imaging of target organs using fluorescent protein transgenes or dye staining. Software packages for the quantification of these fluorescent signals are available from both commercial and noncommercial sources; however, their algorithms are complicated and their resources (code) have mostly not been openly shared. In this study, we developed a simple and robust open-source software tool named "ZF-Mapper" for the quantification of the fluorescence intensity of each pixel in zebrafish images with batch image file processing capability...
March 11, 2019: Zebrafish
Carolyn T Chang, Jet'aime Lewis, Christopher M Whipps
Zebrafish health is a primary research concern because diseases can have unintended impacts on experimental endpoints. Ideally, research would be conducted using disease-free fish or fish with known disease status. Mycobacteriosis is a common bacterial disease in wild and captive fishes, including zebrafish. Despite its prevalence, the dynamics of transmission and potential sources of mycobacterial infections in zebrafish are only partially understood. One suspected natural infection source is surface biofilms on tanks and other system components...
March 5, 2019: Zebrafish
Beata Filipek-Górniok, Johan Ledin, Peter Aleström, Lars Braeutigam
The Nordic zebrafish and husbandry meeting took place at Karolinska Institutet in Stockholm, November 7-9, 2018. More than 120 scientists from Europe joined this meeting, which also attracted world-leading keynote speakers such as Zoltan Varga, Didier Stainier, and Hernán Lopez-Schier. The meeting comprised both scientific as well as zebrafish husbandry and animal welfare sessions. This combination led to fruitful discussions, new collaborations as well as in the formation of a working group that will review and compile evidence-based husbandry guidelines for the local authorities...
March 5, 2019: Zebrafish
Yunyun Jiang, Jiahao Chen, Kuangyu Yen, Jin Xu
Interleukin-34 (IL-34) is a known cytokine that plays an important role in the survival, proliferation, and differentiation of macrophages. In previous studies, IL-34 can induce macrophage migration through syndecan-1 or focal adhesion kinase and extracellular signal-related kinase 1 and 2 pathway. These studies mainly focused on in vitro experiments, but the effect of IL-34 on macrophage migration in vivo is less understood. In our study, we artificially induced macrophage, but not neutrophil, enrichment in the skin or liver by overexpressing IL-34 in epidermal cells or hepatocytes in zebrafish...
February 6, 2019: Zebrafish
Zimei Wu, Ben Koh, Lisa M Lawrence, Manju Kanamala, Bregina Pool, Darren Svirskis, Nicola Dalbeth, Jonathan W Astin, Kathryn E Crosier, Philip S Crosier, Christopher J Hall
Chemical interventions are regularly used to examine and manipulate macrophage function in larval zebrafish. Given chemicals are typically administered by simple immersion or injection, it is not possible to resolve whether their impact on macrophage function is direct or indirect. Liposomes provide an attractive strategy to target drugs to specific cellular compartments, including macrophages. As an example, injecting liposomal clodronate into animal models, including zebrafish, is routinely used to deliver toxic levels of clodronate specifically to macrophages for targeted cell ablation...
February 6, 2019: Zebrafish
Caroline Caetano Da Silva, Tímea Kollár, Zsolt Csenki-Bakos, Bianca H V Fernandes, Ákos Horváth, Luciani R Carvalho
We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20 min) using simple composition solution (200 mM glucose, 40 mM KCl, 30 mM Tris, pH = 8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method...
February 6, 2019: Zebrafish
Nicole M Duff, Robin E Sommerfeld, Matthew K Litvak
Zebrafish (Danio rerio) adults are viewed as sexually dimorphic. However, current approaches to sex discrimination rely mainly on subjective assessment of color patterns and body structures. Here, we explore how geometric morphometrics allow for quantitative sex discrimination based on overall body geometry of adult zebrafish (aged 12-24 months). Ten homologous landmarks were placed on the left lateral view of adult zebrafish and transformed through Procrustes superimposition before being analyzed with canonical variate analysis...
January 29, 2019: Zebrafish
Juan E Franco-Restrepo, Diego A Forero, Rafael A Vargas
The analysis of behavior in animal models is an important objective in many research fields, including neuroscience, psychology, toxicology, and neuropsychopharmacology. Animal models have been used for many years, and several behavioral paradigms, such as locomotor activity, social interactions, and cognitive behavior, have been studied in animal models to correlate the behaviors with pharmacological or environmental interventions and with molecular, biochemical, and physiological findings. We reviewed the literature looking for open-source, freely available software to analyze animal behavior and found 12 freely available programs: ToxTrack, EthoWatcher, Mouse Behavior Tracker, Mouse Move, JAABA, wrMTrck, AnimalTracker, idTracker, Ctrax, Mousetracker, VideoHacking, and Cowlog, which were developed with different programs, work on different platforms, and have particular types of inputs and outputs and analysis capabilities...
January 9, 2019: Zebrafish
Jose González-Fraga, Victor Dipp-Alvarez, Ulises Bardullas
Recently, the evaluation of spontaneous tail movement (STM) in zebrafish embryos has been proposed as a possible neurotoxicity marker in studies on behavioral changes due to chemical exposure. Currently, automated STM quantification can only be performed using high-cost commercial software. We have developed a simple and reliable MATLAB® script called ZebraSTM that allows the automatic analysis of STM in multiple zebrafish embryos.
January 7, 2019: Zebrafish
Rafael A Lara, Raquel O Vasconcelos
Zebrafish is a well-established model organism in hearing research. Although the acoustic environment is known to shape the structure and sensitivity of auditory systems, there is no information on the natural soundscape of this species. Moreover, zebrafish are typically reared in large-scale housing systems (HS), although their acoustic properties and potential effects on hearing remain unknown. We characterized the soundscape of both zebrafish natural habitats and laboratory captive conditions, and discussed possible impact on auditory sensitivity...
December 26, 2018: Zebrafish
Mustafa Hamimi, Mitra Khabooshan, Hozana A Castillo, Jan Kaslin
Gene editing using clustered regularly interspaced short palindromic repeats (CRISPR) is widely used throughout the zebrafish community for the generation of knockouts and knockins. One of the bottlenecks that exists during the process is the laborious screening of injected embryos for F0 founder fish or CRISPants, weeks after the injection date. In this study we show that the use of fluorescently tagged tracrRNA and sorting for fluorescent embryos as early as the 512-cell stage using stereomicroscope significantly improve yield of fish with successfully CRISPR/Cas9-edited genomes...
December 26, 2018: Zebrafish
Gil Martins, Patrícia Diogo, Wilson Pinto, Paulo J Gavaia
Zebrafish is a model species with a high variability of feeding regimes among fish facilities. The use of live feeds for early life stages is a common practice, and few studies have focused early weaning into microdiets. The lack of standardized feeding protocols among research facilities promotes discrepancies in biological performances, and few studies relate dietary regimes to zebrafish development. The objective of this work was to assess the effect of an early transition into microdiets in zebrafish development by evaluating growth, survival, reproductive performance, and skeletal anomalies...
December 18, 2018: Zebrafish
Jonathan Pena Castro, Ricardo Shohei Hattori, Túlio Teruo Yoshinaga, Duílio Mazzoni Zerbinato de Adrade Silva, Fausto Foresti, Mateus Henrique Santos, Mara Cristina Almeida, Roberto Ferreira Artoni
Astyanax is an abundant fish genus in South America. Some species of this group are characterized by the presence of B chromosomes and absence of morphologically differentiated sex chromosomes. In this study, we used quantitative real-time polymerase chain reaction to characterize mRNA expression of dmrt1 in Astyanax scabripinnis gonads. Maturing gonads of males with the B chromosome overexpressed dmrt1. Our findings suggest that B chromosomes may have an adaptive role in A. scabripinnis sex determination and maintenance...
December 18, 2018: Zebrafish
Aldana P David, Brenda Delfín, Magela Rodao, Roberto Rodríguez-Ortiz
The V Latin American Zebrafish Network Course and Symposium was held from May 4 to 12, 2018 at the Institute of Biotechnology of the National Autonomous University of Mexico. A group of 19 students from eight different countries were selected and trained during a week in a variety of topics and techniques using zebrafish as a model, followed by a 2-day symposium. In this article, we want to point out not only the outstanding activities carried out during the course but also highlight the great experience, the knowledge, and the fantastic relationships we gained from those days...
December 4, 2018: Zebrafish
Patrícia Diogo, Gil Martins, Ana Eufrásio, Tomé Silva, Elsa Cabrita, Paulo Gavaia
Selection criteria for sperm cryopreservation are highly relevant in zebrafish since sperm quality is particularly variable in this species. Successful cryopreservation depends on high-quality sperm, which can only be ensured by the selection of breeders. Consequently, male selection and management are a priority to improve cryopreservation, and therefore, this study aimed to characterize optimal age and sperm collection frequency in zebrafish. For this purpose, males from wild type (AB) and from a transgenic line [Tg(runx2:eGFP)] were sampled at 6, 8, 12, and 14 months...
December 4, 2018: Zebrafish
Hemagowri Venkatasubramanian, Velanganni Selvaraj, Mohankumar Ramasamy, Kirankumar Santhakumar
Induction of all-male population in Siamese fighting fish, Betta splendens, has potential application in ornamental fish trade. In addition, the sexually dimorphic nature of aggressive behavior exhibited by this species has made it into an emerging model for behavioral studies. Fadrozole, an aromatase inhibitor, which has been used widely in masculinization, has captivated us to use it in this study. Twenty one days postfertilization (dpf), B. splendens fry were subjected to discrete immersion treatment with various concentrations of fadrozole, and eventually, analyses of various socioreproductive behaviors and analyses of stress markers such as dopamine in brain samples, sex hormones, cortisol, and glucose in plasma samples were performed...
December 4, 2018: Zebrafish
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