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Plant Biotechnology Journal

Jun Cheng, Mengmeng Zhang, Bin Tan, Yajun Jiang, Xianbo Zheng, Xia Ye, Zijing Guo, Tingting Xiong, Wang Wei, Jidong Li, Jiancan Feng
Plant stature is one important factor that affects the productivity of peach orchards. However, little is known about the molecular mechanism(s) underlying the dwarf phenotype of peach tree. Here, we report a dwarfing mechanism in the peach cv. FenHuaShouXingTao (FHSXT). The dwarf phenotype of 'FHSXT' was caused by shorter cell length compared to the standard cv. QiuMiHong (QMH). 'FHSXT' contained higher endogenous GA levels than did 'QMH' and did not response to exogenous GA treatment (internode elongation)...
February 18, 2019: Plant Biotechnology Journal
Jian Ma, Yongfei Wang, Xiaoding Ma, Lingzhi Meng, Ruonan Jing, Fan Wang, Shuai Wang, Zhijun Cheng, Xin Zhang, Ling Jiang, Jiulin Wang, Jie Wang, Zhichao Zhao, Xiuping Guo, Qibing Lin, Fuqing Wu, Shanshan Zhu, Wu Chuanyin, Yulong Ren, Cailin Lei, Huqu Zhai, Jianmin Wan
Lesion mimic mutants that exhibit spontaneous hypersensitive response (HR)-like necrotic lesions are ideal experimental systems for elucidating molecular mechanisms involved in plant cell death and defense responses. Here we report identification of a rice lesion mimic mutant, spotted leaf 35 (spl35), and cloning of the causal gene by TAIL-PCR strategy. spl35 exhibited decreased chlorophyll content, higher accumulation of H2 O2 , up-regulated expression of defense-related marker genes, and enhanced resistance to both fungal and bacterial pathogens of rice...
February 16, 2019: Plant Biotechnology Journal
Philippe V Jutras, Friederike Grosse-Holz, Farnusch Kaschani, Markus Kaiser, Dominique Michaud, Renier A L van der Hoorn
Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed Activity-based Protein Profiling (ABPP) to identify proteases that are inhibited by SlCYS8 in agroinfiltrated Nicotiana benthamiana. We discovered that SlCYS8 selectively suppresses papain-like cysteine protease (PLCP) activity in both apoplastic fluids and total leaf extracts, while not affecting vacuolar processing enzyme and serine hydrolase activity...
February 11, 2019: Plant Biotechnology Journal
Zhengyan Dai, Jiang Tan, Cong Zhou, Xiaofang Yang, Fang Yang, Shijuan Zhang, Shichen Sun, Xuexia Miao, Zhenying Shi
Multi-functional microRNAs (miRNAs) are emerging as key modulators of plant-pathogen interactions. Although the involvement of some miRNAs in plant-insect interactions has been revealed, the underlying mechanisms are still elusive. The brown planthopper (BPH) is the most notorious rice (Oryza sativa)-specific insect that causes severe yield losses each year and requires urgent biological control. To reveal the miRNAs involved in rice-BPH interactions, we performed miRNA sequencing and identified BPH-responsive OsmiR396...
February 7, 2019: Plant Biotechnology Journal
Lauren E Seber Kasinger, Matthew W Dent, Garima Mahajan, Krystal Teasley Hamorsky, Nobuyuki Matoba
The discovery of broadly neutralizing antibodies (bNAbs) has been a major step towards better prophylactic and therapeutic agents against human immunodeficiency virus type 1 (HIV-1). However, effective therapy will likely require a combination of anti-HIV agents to avoid viral evasion. One possible solution to this problem is the creation of bispecific molecules that can concurrently target two vulnerable sites providing synergistic inhibitory effects. Here, we describe the production in plants and anti-HIV activity of a novel bispecific fusion protein consisting of the antigen-binding fragment (Fab) of the CD4 binding site-specific bNAb VRC01 and the antiviral lectin Avaren, which targets the glycan shield of HIV-1 envelope (VRC01Fab -Avaren)...
February 6, 2019: Plant Biotechnology Journal
Ajith Anand, Emily Wu, Zhi Li, Sue TeRonde, Maren Arling, Brian Lenderts, Jasdeep S Mutti, William Gordon-Kamm, Todd J Jones, N Doane Chilcoat
An efficient Agrobacterium-mediated site-specific integration (SSI) technology using the FLP/FRT system in elite maize inbred lines is described. The system allows precise integration of a single copy of a donor DNA flanked by heterologous FRT sites into a predefined recombinant target line (RTL) containing the corresponding heterologous FRT sites. A promoter-trap system consisting of a pre-integrated promoter followed by an FRT site enables efficient selection of events. The efficiency of this system is dependent on several factors including Agrobacterium tumefaciens strain, expression of morphogenic genes Babyboom (Bbm) and Wuschel2 (Wus2) and choice of heterologous FRT pairs...
February 1, 2019: Plant Biotechnology Journal
Zhengzhi Zhang, Lei Hua, Ajay Gupta, David Tricoli, Keith J Edwards, Bing Yang, Wanlong Li
CRISPR/Cas9 has been widely used for genome editing in many organisms, including important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the application of this powerful genome editing tool also depends on DNA delivery methods. In wheat, the biolistics based transformation is the most used method for delivery of the CRISPR/Cas9 complex. Due to the high frequency of gene silencing associated with high copy transgenes and low edit rate in wheat, a large T0 transgenic plant population are required for recovery of desired mutations, which poses a bottleneck for many genome editing projects...
February 1, 2019: Plant Biotechnology Journal
Ragavendran Abbai, Vikas Kumar Singh, Vishnu Varthini Nachimuthu, Pallavi Sinha, Ramchander Selvaraj, Abhilash Kumar Vipparla, Arun Kumar Singh, Uma Maheshwar Singh, Rajeev K Varshney, Arvind Kumar
Though several genes governing various major traits have been reported in rice, their superior haplotype combinations for developing ideal variety remains elusive. In this study, haplotype analysis of 120 previously functionally characterized genes, influencing grain yield (87 genes) and grain quality (33 genes) revealed significant variations in the 3K rice genome (RG) panel. For selected genes, meta-expression analysis using already available datasets along with co-expression network provided insights at systems level...
January 30, 2019: Plant Biotechnology Journal
Xiaolong Li, Jugpreet Singh, Mengfan Qin, Siwei Li, Xun Zhang, Mingyue Zhang, Awais Khan, Shaoling Zhang, Jun Wu
Pear (Pyrus; 2n = 34), the third most important temperate fruit crop, has great nutritional and economic value. Despite the availability of many genomic resources in pear, it is challenging to genotype novel germplasm resources and breeding progeny in a timely and cost-effective manner. Genotyping arrays can provide fast, efficient, and high-throughput genetic characterization of diverse germplasm, genetic mapping and breeding populations. We present here 200K AXIOM® PyrSNP, a large-scale single nucleotide polymorphism (SNP) genotyping array to facilitate genotyping of Pyrus species...
January 28, 2019: Plant Biotechnology Journal
Gunvant B Patil, Naoufal Lakhssassi, Jinrong Wan, Li Song, Zhou Zhou, Mariola Klepadlo, Tri D Vuong, Adrian O Stec, Sondus S Kahil, Vincent Colantonio, Babu Valliyodan, Hollis J Rice, Sarbottam Piya, Tarek Hewezi, Robert M Stupar, Khalid Meksem, Henry T Nguyen
Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance derived from PI88788 is breaking down due to narrow genetic background and SCN population shift. PI88788 requires mainly the rhg1-b locus, while 'Peking' requires rhg1-a and Rhg4 for SCN resistance. In the present study, whole-genome sequencing of 106 soybean lines was used to define the Rhg haplotypes and investigate their responses to the SCN HG-Types...
January 28, 2019: Plant Biotechnology Journal
Deyong Ren, Qiankun Xu, Zhennan Qiu, Yuanjiang Cui, Tingting Zhou, Dali Zeng, Longbiao Guo, Qian Qian
The spikelet is the specific unit of the grass inflorescence, and produces a variable number of florets (1-40) depending on whether the species displays determinate or indeterminate growth. Spikelet morphogenesis therefore plays an important role in crop yield; for example, in wheat (Triticum aestivum) inflorescences with the indeterminate spikelet, the spikelet meristem is continuously active and produces a plethora of lateral floral meristems, leading to the formation of variable numbers of florets. In inflorescences of rice (Oryza sativa) and maize (Zea mays) with the determinate spikelet, the spikelet meristem produces the fixed numbers of lateral floral meristems, after which its fate is terminated as a terminal floral meristem, resulting in the formation of one or two florets...
January 24, 2019: Plant Biotechnology Journal
Fabio Pasin, Wulf Menzel, José-Antonio Daròs
Recent metagenomic studies have provided an unprecedented wealth of data, which is revolutionizing our understanding of virus diversity. A redrawn landscape highlights viruses as active players in the phytobiome, and surveys have uncovered their positive roles in environmental stress tolerance of plants. Viral infectious clones are key tools for functional characterization of known and newly identified viruses. Knowledge of viruses and their components has been instrumental for the development of modern plant molecular biology and biotechnology...
January 24, 2019: Plant Biotechnology Journal
Ashish Ranjan, Nathaniel M Westrick, Sachin Jain, Jeff S Piotrowski, Manish Ranjan, Ryan Kessens, Logan Stiegman, Craig R Grau, Shawn P Conley, Damon L Smith, Mehdi Kabbage
Sclerotinia sclerotiorum, a predominately necrotrophic fungal pathogen with a broad host range, causes a significant yield limiting disease of soybean called Sclerotinia stem rot. Resistance mechanisms against this pathogen in soybean are poorly understood, thus hindering the commercial deployment of resistant varieties. We used a multiomic approach utilizing RNA-sequencing, Gas chromatography-mass spectrometry-based metabolomics and chemical genomics in yeast to decipher the molecular mechanisms governing resistance to S...
January 22, 2019: Plant Biotechnology Journal
Thomas Rademacher, Markus Sack, Daniel Blessing, Rainer Fischer, Tanja Holland, Johannes Buyel
Industrial plant biotechnology applications include the production of sustainable fuels, complex metabolites and recombinant proteins, but process development can be impaired by a lack of reliable and scalable screening methods. Here we describe a rapid and versatile expression system which involves the infusion of Agrobacterium tumefaciens into three-dimensional, porous plant cell aggregates deprived of cultivation medium, which we have termed plant cell packs (PCPs). This approach is compatible with different plant species such as Nicotiana tabacum BY2, Nicotiana benthamiana or Daucus carota and 10-times more effective than transient expression in liquid plant cell culture...
January 22, 2019: Plant Biotechnology Journal
Yanpei Zhang, Zhanying Zhang, Xingming Sun, Xiaoyang Zhu, Ben Li, Jinjie Li, Haifeng Guo, Chao Chen, Yinghua Pan, Yuntao Liang, Zhijian Xu, Hongliang Zhang, Zichao Li
Rice (Oryza sativa L.) cultivars harbor morphological and physiological traits different from those of wild rice (O. rufipogon Griff.), but the molecular mechanisms underlying domestication remain controversial. Here, we show that awn and long grain traits in the near-isogenic NIL-GLA are separately controlled by variations within the GLA (Grain Length and Awn Development) gene, a new allele of GAD1/RAE2, which encodes one member of the EFPL (epidermal patterning factor-like protein) family. Haplotype analyses and transgenic studies revealed that InDel1 (variation for grain length, VGL) in the promoter region of GLA (GLAVGL ) increases grain length by promoting transcription of GLA...
January 21, 2019: Plant Biotechnology Journal
Anja Taubert, Torsten Jakob, Christian Wilhelm
Glycolate is produced in autotrophic cells under high temperatures and Ci -limitation via oxygenation of ribulose-1,5-bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C2 cycle by consuming reductants, ATP and CO2 emission (photorespiration). Therefore, photorespiration is an inhibitory process for biomass production. However, cells can be manipulated in a way that they become glycolate producing "cell factories", when the ratio carboxylation/oxygenation is 2...
January 13, 2019: Plant Biotechnology Journal
András Kis, Éva Hamar, Gergely Tholt, Rita Bán, Zoltan Havelda
Wheat dwarf virus (WDV) is an economically important, phloem-limited, insect-transmitted virus belonging to the Geminiviridae family (Tholt et al., 2018). WDV strains infect both wheat and barley causing severe yield losses and the natural resistance resources are limited (Nygren et al., 2015). Direct utilization of the CRISPR/Cas9 system to inhibit geminivirus replication has been described in model plants (Zaidi et al., 2016). Here we show the direct anti-viral utilization of the CRISPR/Ca9 system in an important crop plant, barley (Hordeum vulgare L...
January 11, 2019: Plant Biotechnology Journal
Jia-Wu Feng, Shanshan Huang, Yi-Xiong Guo, Dongxu Liu, Jia-Ming Song, Junxiang Gao, Huan Li, Ling-Ling Chen
In higher eukaryotes, alternative splicing (AS) and alternative polyadenylation (APA) events can produce multiple transcript isoforms in the majority of genes, which significantly increase the protein-coding potential of a genome (Pan et al., 2008; Anvar et al., 2018). Different transcript isoforms might encode proteins with different functions or affect the mRNA stability and translational capacity, in some sense AS and APA events can dramatically increase the complexity and flexibility of the entire transcriptome and proteome (Yang et al...
January 11, 2019: Plant Biotechnology Journal
Xin Ji, Yanxiu Du, Fei Li, Hongzheng Sun, Jing Zhang, Junzhou Li, Ting Peng, Zeyu Xin, Quanzhi Zhao
As members of the basic helix-loop-helix transcription factor families, phytochrome-interacting factors (PIFs) regulate an array of developmental responses ranging from seed germination to plant growth. However, little is known about their roles in modulating grain development. Here we firstly analyzed the expression pattern of rice OsPIL genes in grains and found that OsPIL15 may play an important role in grain development. We then generated knock-out (KO) OsPIL15 lines in rice using CRISPR/Cas9 technology, the silencing expression of OsPIL15 led to increased numbers of cells, which thus enhanced grain size and weight...
January 9, 2019: Plant Biotechnology Journal
Jesse T Beasley, Julien P Bonneau, Jose T Sánchez-Palacios, Laura T Moreno-Moyano, Damien L Callahan, Elad Tako, Raymond P Glahn, Enzo Lombi, Alexander A T Johnson
Bread wheat (Triticum aestivum L.) is cultivated on more land than any other crop and produces a fifth of the calories consumed by humans. Wheat endosperm is rich in starch yet contains low concentrations of dietary iron (Fe) and zinc (Zn). Biofortification is a micronutrient intervention aimed at increasing the density and bioavailability of essential vitamins and minerals in staple crops; Fe biofortification of wheat has proved challenging. In this study we employed constitutive expression (CE) of the rice (Oryza sativa L...
January 8, 2019: Plant Biotechnology Journal
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