Scott B Laffoon, James D Doecke, Anne M Roberts, Jennifer A Vance, Benjamin D Reeves, Kelly K Pertile, Rebecca L Rumble, Chris J Fowler, Brett Trounson, David Ames, Ralph Martins, Ashley I Bush, Colin L Masters, Paul A Grieco, Edward A Dratz, Blaine R Roberts
BACKGROUND: The Australian Imaging and Biomarker Lifestyle (AIBL) study of aging is designed to aid the discovery of biomarkers. The current study aimed to discover differentially expressed plasma proteins that could yield a blood-based screening tool for Alzheimer's disease. METHODS: The concentration of proteins in plasma covers a vast range of 12 orders of magnitude. Therefore, to search for medium to low abundant biomarkers and elucidate mechanisms of AD, we immuno-depleted the most abundant plasma proteins and pre-fractionated the remaining proteins by HPLC, prior to two-dimensional gel electrophoresis...
January 26, 2022: Proteome Science