Natural Product Research

A total of 20 endophytic fungi were isolated (ZSEFL1-ZSEFL20) from Ziziphus spina-christi (L.) Desf. (Nabq) leaves. Four isolates A2/ZSEFL2, Alternaria alternata , D/ZSEFL14, Aspergillus niger , E/ZSEFL15, Epicoccum nigrum , and S/ZSEFL19, Penicillium crustosum were found to show the most promising antimicrobial activities either in plug or disc diffusion screening assays against Gram-positive, Gram-negative bacteria and pathogenic fungi. Antimicrobial activity was tested against Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, Serratia marcescens ATCC 14764, Klebsiella pneumonia e ATCC 700603, Candida albicans ATCC 10231, and Fusarium oxysporum ATCC417...

An undescribed dihydrostilbene, macajavanicin D ( 1 ), and three known analogs, malayheyneiin A ( 2 ) and laevifolins A-B ( 3 - 4 ), were isolated from Macaranga javanica (Blume) Müll. Arg. leaves. Macajavanicin D ( 1 ) structure was determined based on a combination of ESI-HRMS data and NMR spectra. Compounds 1-4 were evaluated to Plasmodium falciparum strain 3D7. Macajavanicins D ( 1 ) and laevifolin A ( 3 ) showed potent activity with an IC50 value of 0.85 and 1.03 µg/mL, respectively.

Tripterygium wilfordii has been historically employed as a conventional botanical insecticide and a plant of medicinal significance. A new dihydroagarofuran sesquiterpene ( 1 ) and a new acyclic compound ( 2 ), along with seven known compounds ( 3-9 ), have been isolated from the aerial parts of Tripterygium wilfordii . The identification of the structures of novel compounds were accomplished through comprehensive spectroscopic analyses, encompassing HRESIMS, NMR, UV, IR, and a comparative analysis with spectroscopic data from compounds previously characterised...

Icariin is the most bioactive ingredient of Epimedium L . and a quality marker of Herba Epimedii. Conventional methods for production of Icariin are known to be inefficient, resulting in low yields and significant environmental pollution. This study aimed to develop a sustainable and effective biphasic enzymatic hydrolysis system for the efficient conversion of epimedin C to icariin. The biphasic system was created using butyl acetate and phosphate buffer (pH 4.5) at a ratio of 3:1 ( V/V ) along with α- L -rhamnosidase/epimedin C (2 U/1 mg) at 50 °C for 12 h...

A new cyclopeptide alkaloid, spinachristene A ( 1 ), along with two previously described, sanjoinenine ( 2 ) and oxyphylline C ( 3 ), were isolated from the fruits of Paliurus spina-christi Mill. All three metabolites are being isolated for the first time from the genus Paliurus . A model for the in silico binding affinity of compounds 1 - 3 to Dipeptidyl Peptidase IV (DPP4), which is related to type 2 diabetes (T2D), was developed. According to our model, compounds 1-3 were ranked in positions 9/12, 11/12 and 8/12, respectively and are predicted to exhibit significant affinity to DPP4, in the range of low 2-digit μΜ...

The present study aimed to identify the presence of certain classes of phytochemicals in the leaf extract of medicinal herbs viz. Moringa oleifera and Calotropis procera , using qualitative detection tests and explored the potential of aqueous and ethanolic extract to inhibit aflatoxin production by thin layer chromatography at 25 °C and pH (7) of different incubation times i.e. 0-, 1-, 3-, 6- and 24-h. Qualitative phytochemical analysis reported that the aqueous leave extracts of M. oleifera and C...

Two novel neolignans, piperkadsurenin A ( 1 ) and kadsurenin N ( 2 ), along with six known neolignans ( 3-8 ) and two lignans ( 9-10 ) were isolated from the stems of Piper kadsura (Choisy) Ohwi. Extensive spectroscopic data interpretation and ECD calculations were used to identify the structures of the new compounds 1 and 2 . Especially, compound 1 represents the first example of neolignan with cyclopenta[ b ]pyran framework. The anti-inflammatory efficacy of compounds 1-10 in vitro was systematically assessed through NO production inhibitory assay...

The aim of the study was to investigate the relationship between flavonoids in Abrus precatorius leaves (APL) and their hypoglycaemic effects, which have not been studied before. An efficient purification process, transcriptomics and network pharmacology analysis were applied for the first time. High-performance liquid chromatography (HPLC) was used to determine the content of total flavonoids. The results showed that D101 resin was most suitable for purification of flavonoids of APL, which could increase its purity from 25...

The Limonium genus (Plumbaginaceae) includes several species of perennial herbs and shrubs belonging to a particular type of halophytes, known as 'recretohalophytes'. Limonium species are widely distributed in the Mediterranean region, mainly in the North-Eastern and Southern countries and several bioactivities have been well documented. In the present study, the chemical composition of the essential oil (EO) of the aerial parts of Limonium lobatum (L.f.) Chaz., a species never chemically previously studied and collected in Algeria, which grows in South Spain, North Africa, and SW Asia, was analysed using GC-MS...

Gigantol, a bibenzyl compound extracted from various medicinal plants, has shown a number of biological activities, making it an attractive candidate for potential medical applications. This systematic review aims to shed light on gigantol's promising role in inflammation treatment and its underlying mechanisms. Gigantol exhibits potential anti-inflammatory properties in pre-clinical pharmacological test systems. It effectively reduced the levels of pro-inflammatory markers and arachidonic acid metabolites through various pathways, such as NF-κB, AKT, PI3K, and JNK/cPLA2/12-LOX...

Synthesis, characterisation, and anti-diabetic potential of swertiamarin analogues against DPP-4 enzymatic inhibition was done prior to this study. However, swertiamarin and its analogues inhibited DPP-4 enzyme significantly. Semisynthetic swertiamarin analogues have been studied for antidiabetic potential and mechanism of action utilising molecular docking and in-vitro techniques. The mechanism of action for swertiamarin analogues was determined by in-silico molecular docking studies using glucose-transporters, GLUT-1 (PDB ID: 4PYP), GLUT-3 (PDB ID: 7SPS), and GLUT-4 (PDB ID: 7WSM) along with in-vitro glucose uptake and glucose-induced insulin secretion assays...

This paper reports the isolation of two undescribed phenolic glycosides ( 1 and 2 ), together with seven known compounds ( 3 - 9 ) from the branches of Viburnum chinshanense . The structures of undescribed compounds were elucidated by comprehensive spectroscopic methods (1D NMR, 2D NMR, and HRESIMS). The sugar units of compounds 1 and 2 were identified by acid hydrolysis and HPLC analysis of the chiral derivatives of the monosaccharides. Furthermore, the α ‑amylase and α -glucosidase inhibitory activities of all isolates were evaluated and compounds 1 , 5 , and 8 displayed potential α ‑amylase and α -glucosidase inhibitory activities...

This study is focused on investigating the chemical composition and bioactive properties of the essential oil extracted from Psychotria asiatica L., a plant species known for its medicinal properties. Utilising gas chromatography-mass spectrometry (GC-MS) analysis, the essential oil from P. asiatica was found to contain 53 distinct constituents. Major compounds identified include ( E )-citral (20.6%), 10- epi - γ -eudesmol (15.9%), ( Z )-citral (10.5%), geraniol (7.4%), α -cadinol (6.7%), 7- epi - α -eudesmol (4...

Three new metabolites ( 1 - 3 ) were isolated from the stems of Knema globularia , along with five known compounds, including kaempferol ( 4 ), quercetin ( 5 ), isovanillic acid ( 6 ), protocatechuic acid ( 7 ), and gallic acid ( 8 ). Their structures were deduced using NMR spectroscopic, mass spectrometric analyses, and literature data. The absolute configurations of 1 - 3 were established by electronic circular dichroism (ECD) spectroscopy. α -Glucosidase inhibitory activities of those compounds were evaluated using a spectrophotometric method, compounds 1 - 3 showed very strong effects towards α -glucosidase with IC50 values 1...

A novel polycyclic quinazoline alkaloid ( 1 ) along with one new natural quinoline alkaloid ( 2 ) and two known quinoline alkaloids ( 3 , 4 ) were isolated from the marine-derived fungus Trichoderma longibrachiatum QD01. Structural determinations of those isolates were established by comprehensive spectroscopic analyses and literature comparison. Single-crystal X-ray diffraction analysis of novel compound verified its structure and stereochemistry, representing the first characterised crystal structure of a trimeric-type of tetrahydroquinazoline...

In the present study, the chemical composition of the essential oil from aerial parts of two populations of Paeonia mascula subsp. russoi , collected in Sicily, was evaluated by GC-MS. No previously phytochemical investigation has been reported for this subspecies. The main components of the essential oil of the population with pink flowers were salicylaldehyde (34.31%), nonanal (16.95%) and 2-hexenal (10.17%), whereas essential oil of the population with white flowers, was shown to be rich of myrtanal (14...

The techniques LC-UV-BPSU and LC-UV-SPE/NMR were applied for the first time in the analysis of açai berry ( Euterpe oleracea Mart.) pulp extracts. Those techniques allowed the identification of twenty-three metabolites: Valine (1), citric acid (2), tachioside (3), isotachioside (4), α -guaiacylglycerol (5), syringylglycerol (6), uridine (7), adenosine (8), dimethoxy-1,4-benzoquinone (9), koaburaside (10), protocatechuic acid (11), eurycorymboside B (12), 7',8'-dihydroxy-dihydrodehydroconiferyl alcohol-9- O - β-D -glucopyranoside (13), orientin (14), homoorientin (15), dihydrokaempferol-3-glucoside (16), isolariciresinol-9'- O-β-D -glucopyranoside (17), 5'-methoxyisolariciresinol-9'- O-β-D -glucopyranoside (18), cyanidin-3- O -glucoside (19), cyandin-3- O -rutenoside (20), 9,12-octadecadienoic acid (Z,Z)-2-hydroxy-1-(hydroxymethyl) ethyl ester (21), linolenic acid (22), and 1,2-di- O - α -linolenoyl-3- O-β-D -galactopyranosyl-sn-glycerol (23)...

Camelina sativa (L.) Crantz is an oilseed plant common in Europe and Asia. This study used the gas chromatography-mass spectrometry (GC-MS) to examine the differences in the aroma on the basis of extraction method such as water distillation extraction (CSPW), Solid-phase microextraction (CSPM) and subcritical extraction (CSPS). Antibacterial test was evaluated by the microdilution method against Salmonella typhimurium , Streptococcus pneumoniae , Escherichia coli , Strepococcus pyogenens , Staphylococcus aureus , and antioxidant activity was determined through DPPH free radical, hydroxyl free radical, and superoxide anion radical scavenging capacity activity...

Vascular system is essential for the body to maintain health. Dysregulated vascular system leads to cardiovascular diseases and are observed in ischaemic stroke, Alzheimer's disease, amyotrophic lateral sclerosis, and diabetes. TIE2 is a tyrosine kinase receptor expressed on vascular endothelial cells and contributes to the maintenance of a vascular system. In this paper, we screened for natural products with an activity to induce phosphorylation of TIE2, which will be beneficial for protection of a vascular system...

In this study, the effects of natural waste products such as extracts from C. coggygria leaves and Punica granatum L. peels were investigated against oxidative DNA damage induced by Fenton reaction. 8-OH-2'dG as a general marker of DNA damage on thymus DNA, and the bioactive compounds of extracts were measured by LC-MS/MS. Our results had shown that ethanol extracts of C. coggygria leaf and Punica granatum L. peel had a protective effect on oxidative damaged DNA. It was determined that the bioactive compounds of C...