Journals Annals of Clinical Microbiolog...

Annals of Clinical Microbiology and Antimicrobials
Mathira Wongchai, Saharut Wongkaewkhiaw, Sakawrat Kanthawong, Sittiruk Roytrakul, Ratchaneewan Aunpad
BACKGROUND: Due to their resistance and difficulty in treatment, biofilm-associated infections are problematic among hospitalized patients globally and account for 60% of all bacterial infections in humans. Antibiofilm peptides have recently emerged as an alternative treatment since they can be effectively designed and exert a different mode of biofilm inhibition and eradication. METHODS: A novel antibiofilm peptide, BiF, was designed from the conserved sequence of 18 α-helical antibiofilm peptides by template-assisted technique and its activity was improved by hybridization with a lipid binding motif (KILRR)...
May 16, 2024: Annals of Clinical Microbiology and Antimicrobials
Nathalie Yepes Madrid, Luis Fernando Mejia, José Fernando Gomez Urrego
BACKGROUND: Shewanella putrefaciens is a gram-negative, nonfermenting, oxidase-positive, hydrogen sulfide-producing bacillus and a halophilic bacterium, known for causing unusual infections in humans and often regarded as an opportunistic pathogen. Its diverse symptoms have a significant impact on human health, with 260 documented disorders reported in the literature over the last 40 years, highlighting its potential danger. CASE PRESENTATION: We present the case of a previously healthy 15-year-old male patient who sustained a self-inflicted sharp-object injury while working in the field, resulting in secondary septic monoarthritis due to Shewanella putrefaciens...
May 10, 2024: Annals of Clinical Microbiology and Antimicrobials
Mariana Guedes, David Gathara, Inmaculada López-Hernández, Pedro María Martínez Pérez-Crespo, María Teresa Pérez-Rodríguez, Adrian Sousa, Antonio Plata, Jose María Reguera-Iglesias, Lucía Boix-Palop, Beatriz Dietl, Juan Sevilla Blanco, Carlos Armiñanzas Castillo, Fátima Galán-Sánchez, Clara Natera Kindelán, Alfredo Jover-Saenz, Josune Goikoetxea Aguirre, Ana Alemán Alemán, Teresa Marrodán Ciordia, Alfonso Del Arco Jiménez, Jonathan Fernandez-Suarez, Luis Eduardo Lopez-Cortes, Jesús Rodríguez-Baño
BACKGROUND: Klebsiella aerogenes has been reclassified from Enterobacter to Klebsiella genus due to its phenotypic and genotypic similarities with Klebsiella pneumoniae. It is unclear if clinical outcomes are also more similar. This study aims to assess clinical outcomes of bloodstreams infections (BSI) caused by K. aerogenes, K. pneumoniae and Enterobacter cloacae, through secondary data analysis, nested in PRO-BAC cohort study. METHODS: Hospitalized patients between October 2016 and March 2017 with monomicrobial BSI due to K...
May 6, 2024: Annals of Clinical Microbiology and Antimicrobials
Ziran Wang, Danping Liu, Jingjia Zhang, Lingli Liu, Zeming Zhang, Chang Liu, Songnian Hu, Linhuan Wu, Zilong He, Hongli Sun
BACKGROUND: Infections caused by linezolid-resistant enterococci (LRE) are clinically difficult to treat and threaten patient health. However, there is a lack of studies on long time-span LRE strains in China. For this reason, our study comprehensively revealed the resistance mechanisms of LRE strains collected in a Chinese tertiary care hospital from 2011 to 2022. METHODS: Enterococcal strains were screened and verified after retrospective analysis of microbial data...
May 4, 2024: Annals of Clinical Microbiology and Antimicrobials
Madjid Morsli, Florian Salipante, Chloé Magnan, Catherine Dunyach-Remy, Albert Sotto, Jean-Philippe Lavigne
BACKGROUND: Non-surgical chronic wounds, including diabetes-related foot diseases (DRFD), pressure injuries (PIs) and venous leg ulcers (VLU), are common hard-to-heal wounds. Wound evolution partly depends on microbial colonisation or infection, which is often confused by clinicians, thereby hampering proper management. Current routine microbiology investigation of these wounds is based on in vitro culture, focusing only on a limited panel of the most frequently isolated bacteria, leaving a large part of the wound microbiome undocumented...
May 3, 2024: Annals of Clinical Microbiology and Antimicrobials
Bing Zhao, Huiwen Zheng, Juliano Timm, Zexuan Song, Shaojun Pei, Ruida Xing, Yajie Guo, Ling Ma, Feina Li, Qing Li, Yan Li, Lin Huang, Chong Teng, Ni Wang, Aastha Gupta, Sandeep Juneja, Fei Huang, Yanlin Zhao, Xichao Ou
BACKGROUND: Pretomanid is a key component of new regimens for the treatment of drug-resistant tuberculosis (TB) which are being rolled out globally. However, there is limited information on the prevalence of pre-existing resistance to the drug. METHODS: To investigate pretomanid resistance rates in China and its underlying genetic basis, as well as to generate additional minimum inhibitory concentration (MIC) data for epidemiological cutoff (ECOFF)/breakpoint setting, we performed MIC determinations in the Mycobacterial Growth Indicator Tube™ (MGIT) system, followed by WGS analysis, on 475 Mycobacterium tuberculosis (MTB) isolated from Chinese TB patients between 2013 and 2020...
May 3, 2024: Annals of Clinical Microbiology and Antimicrobials
Jie Ma, Ranran Xu, Wanxiang Li, Mi Liu, Xiaomei Ding
OBJECTIVE: To analyze the clinical infection characteristics and genetic environments of resistance genes in carbapenem-resistant Citrobacter europaeus using whole-genome sequencing. METHODS: The susceptibility of two clinical isolates of C. europaeus (WF0003 and WF1643) to 24 antimicrobial agents was assessed using the BD Phoenix™ M50 System and Kirby-Bauer (K-B) disk-diffusion method. Whole-genome sequencing was performed on the Illumina and Nanopore platforms, and ABRicate software was used to predict resistance and virulence genes of carbapenem-resistant C...
April 29, 2024: Annals of Clinical Microbiology and Antimicrobials
Rym Charfi, Cécile Guyonnet, Meiggie Untrau, Gaëlle Giacometti, Thierry Paper, Claire Poyart, Céline Plainvert, Asmaa Tazi
PURPOSE: Group B Streptococcus (GBS) is the leading cause of invasive infections in newborns. The prevention of GBS neonatal disease relies on the administration of an intrapartum antibiotic prophylaxis to GBS-colonized women. In recent years, rapid intrapartum detection of GBS vaginal colonization using real-time nucleic acid amplification tests (NAATs) emerged as an alternative to antenatal culture screening methods. METHODS: We compared the performances of two loop-mediated isothermal amplification (LAMP) tests, the Ampliflash® GBS and the PlusLife® GBS tests, to standard culture for GBS detection in vaginal specimens from pregnant women...
April 25, 2024: Annals of Clinical Microbiology and Antimicrobials
Nahid Madadi-Goli, Kamal Ahmadi, Mansour Kargarpour Kamakoli, Mohsen Azizi, Sharareh Khanipour, Shahin Pourazar Dizaji, Mahshid Nasehi, Seyed Davar Siadat, Abolfazl Fateh, Farzam Vaziri
BACKGROUND: Tuberculosis (TB) continues to pose a threat to communities worldwide and remains a significant public health issue in several countries. We assessed the role of heteroresistance and efflux pumps in bedaquiline (BDQ)-resistant Mycobacterium tuberculosis isolates. METHODS: Nineteen clinical isolates were included in the study, of which fifteen isolates were classified as MDR or XDR, while four isolates were fully susceptible. To evaluate BDQ heteroresistance, the Microplate Alamar Blue Assay (MABA) method was employed...
April 25, 2024: Annals of Clinical Microbiology and Antimicrobials
Dae Hyeon Cho, Si-Ho Kim, Cheon Hoo Jeon, Hyoung Tae Kim, Kyoung-Jin Park, Junyoung Kim, Jiyeong Kwak, Byung Soo Kwan, Sungmin Kong, Jung Won Lee, Kwang Min Kim, Yu Mi Wi
PURPOSE: The clinical significance of negative toxin enzyme immunoassays (EIA) for Clostridioides difficile infections (CDIs) is unclear. Our study aimed to investigate the significance of toxin EIA-negative in the diagnosis and prognosis of CDI. METHODS: All stool specimens submitted for C. difficile toxin EIA testing were cultured to isolate C. difficile. In-house PCR for tcdA, tcdB, cdtA, and cdtB genes were performed using C. difficile isolates. Stool specimens were tested with C...
April 25, 2024: Annals of Clinical Microbiology and Antimicrobials
Oscar Backrud, Erik Engberg, Kristina Nyberg, Peter Wieslander, Edward R B Moore
BACKGROUND: Chromobacterium is a genus of fourteen species with validly published names, most often found in soil and waters in tropical and subtropical regions around the world. The most well-known species of the genus, C. violaceum, occasionally causes clinically relevant infections; cases of soft tissue infections with septicemia and fatal outcomes have been described. CASE PRESENTATION: Here, we present a clinical case report of a 79-year-old man from Sweden with a soft-tissue infection and septicemia...
April 18, 2024: Annals of Clinical Microbiology and Antimicrobials
Mingyu Gan, Yanyan Zhang, Gangfeng Yan, Yixue Wang, Guoping Lu, Bingbing Wu, Weiming Chen, Wenhao Zhou
BACKGROUND: Antimicrobial resistance (AMR) is a major threat to children's health, particularly in respiratory infections. Accurate identification of pathogens and AMR is crucial for targeted antibiotic treatment. Metagenomic next-generation sequencing (mNGS) shows promise in directly detecting microorganisms and resistance genes in clinical samples. However, the accuracy of AMR prediction through mNGS testing needs further investigation for practical clinical decision-making. METHODS: We aimed to evaluate the performance of mNGS in predicting AMR for severe pneumonia in pediatric patients...
April 15, 2024: Annals of Clinical Microbiology and Antimicrobials
Chongyang Wu, Li Xiong, Quanfeng Liao, Weili Zhang, Yuling Xiao, Yi Xie
BACKGROUND: Elizabethkingia is emerging as an opportunistic pathogen in humans. The aim of this study was to investigate the clinical epidemiology, antimicrobial susceptibility, virulence factors, and genome features of Elizabethkingia spp. METHODS: Clinical data from 71 patients who were diagnosed with Elizabethkingia-induced pneumonia and bacteremia between August 2019 and September 2021 were analyzed. Whole-genome sequencing was performed on seven isolates, and the results were compared with a dataset of 83 available Elizabethkingia genomes...
April 10, 2024: Annals of Clinical Microbiology and Antimicrobials
Pranjal Kumar Yadav, Suchetna Singh, Moumita Paul, Sanjay Kumar, S Ponmariappan, Duraipandian Thavaselvam
BACKGROUND: Melioidosis, caused by the category B biothreat agent Burkholderia pseudomallei, is a disease with a high mortality rate and requires an immediate culture-independent diagnosis for effective disease management. In this study, we developed a highly sensitive qPCR assay for specific detection of Burkholderia pseudomallei and melioidosis disease diagnosis based on a novel target sequence. METHODS: An extensive in-silico analysis was done to identify a novel and highly conserved sequence for developing a qPCR assay...
April 10, 2024: Annals of Clinical Microbiology and Antimicrobials
Reham Talaat, Mohamed N Abu El-Naga, Heba Abd Alla El-Bialy, Mohie Z El-Fouly, Mohamed A Abouzeid
BACKGROUND: Anti-virulence therapy is a promising strategy to treat multi-drug resistant (MDR) pathogens. Pseudomonas aeruginosa is a potent opportunistic pathogen because of an array of virulence factors that are regulated by quorum sensing systems. METHODS: The virulence features of four multi-drug resistant P. aeruginosa strains were investigated upon exposure to the sub-lethal dose of gamma rays (1 kGy), and sub-inhibitory concentrations of bioactive metabolites recovered from local halophilic strains in comparison to control...
April 10, 2024: Annals of Clinical Microbiology and Antimicrobials
Yu Jeong Choi, Yoonjung Kim, Hye Jung Park, Dokyun Kim, Hyukmin Lee, Young Ah Kim, Kyung-A Lee
BACKGROUND: The prevalence of multidrug-resistant tuberculosis (MDR-TB) among Korean tuberculosis patients is about 4.1%, which is higher than the OECD average of 2.6%. Inadequate drug use and poor patient compliance increase MDR-TB prevalence through selective pressure. Therefore, prompt detection of drug resistance in tuberculosis patients at the time of diagnosis and quantitative monitoring of these resistant strains during treatment are crucial. METHODS: A multiplex droplet digital PCR (ddPCR) assay was developed and assessed using DNA material of nine Mycobacterium tuberculosis strains with known mutation status that were purchased from the Korean National Tuberculosis Association...
April 5, 2024: Annals of Clinical Microbiology and Antimicrobials
Dao Thi Huyen, Julien Reboud, Dao Thanh Quyen, Jonathan M Cooper, Thirumalaisamy P Velavan, Ngo Tat Trung, Le Huu Song
BACKGROUND: Neisseria meningitidis can cause life-threatening meningococcal meningitis and meningococcemia. Old standard microbiological results from CSF/blood cultures are time consuming. This study aimed to combine the sensitivity of loop-mediated isothermal nucleic acid amplification (LAMP) with the specificity of CRISPR/Cas12a cleavage to demonstrate a reliable diagnostic assay for rapid detection of N. meningitidis. METHODS: A total of n = 139 samples were collected from patients with suspected meningococcal disease and were used for evaluation...
March 30, 2024: Annals of Clinical Microbiology and Antimicrobials
Qian Wang, Yue Liu, Ran Chen, Meng Zhang, Zaifeng Si, Yueling Wang, Yan Jin, Yuanyuan Bai, Zhen Song, Xinglun Lu, Mingju Hao, Yingying Hao
BACKGROUND: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) co-producing blaKPC and blaNDM poses a serious threat to public health. This study aimed to investigate the mechanisms underlying the resistance and virulence of CR-hvKP isolates collected from a Chinese hospital, with a focus on blaKPC and blaNDM dual-positive hvKP strains. METHODS: Five CR-hvKP strains were isolated from a teaching hospital in China. Antimicrobial susceptibility and plasmid stability testing, plasmid conjugation, pulsed-field gel electrophoresis, and whole-genome sequencing (WGS) were performed to examine the mechanisms of resistance and virulence...
March 29, 2024: Annals of Clinical Microbiology and Antimicrobials
Narjess Bostanghadiri, Mohammad Sholeh, Tahereh Navidifar, Leila Dadgar-Zankbar, Zahra Elahi, Alex van Belkum, Davood Darban-Sarokhalil
INTRODUCTION: Infections caused by Stenotrophomonas maltophilia are clinically important due to its intrinsic resistance to a broad range of antibiotics. Therefore, selecting the most appropriate antibiotic to treat S. maltophilia infection is a major challenge. AIM: The current meta-analysis aimed to investigate the global prevalence of antibiotic resistance among S. maltophilia isolates to the develop more effective therapeutic strategies. METHOD: A systematic literature search was performed using the appropriate search syntax after searching Pubmed, Embase, Web of Science and Scopus databases (May 2023)...
March 19, 2024: Annals of Clinical Microbiology and Antimicrobials
Hyun-Eui Park, Kyu-Min Kim, Minh Phuong Trinh, Jung-Wan Yoo, Sung Jae Shin, Min-Kyoung Shin
BACKGROUND: Mycobacterium avium complex (MAC) is a group of slow-growing mycobacteria that includes Mycobacterium avium and Mycobacterium intracellulare. MAC pulmonary disease (MAC-PD) poses a threat to immunocompromised individuals and those with structural pulmonary diseases worldwide. The standard treatment regimen for MAC-PD includes a macrolide in combination with rifampicin and ethambutol. However, the treatment failure and disease recurrence rates after successful treatment remain high...
March 18, 2024: Annals of Clinical Microbiology and Antimicrobials
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