Guiyue Tang, Zilong Zhang, Wei Tan, Fei Long, Jingxian Sun, Yingying Li, Siwei Zou, Yujiao Yang, Kezhu Cai, Shenwei Li, Zhiyi Wang, Jiakun Liu, Guobing Mao, Yingxin Ma, Guo-Ping Zhao, Zhen-Gan Tian, Wei Zhao
Timely and accurate detection of SARS-CoV-2 variants of concern (VOCs) is urgently needed for pandemic surveillance and control. Great efforts have been made from a mass of scientists in increasing the detection sensitivity and operability, and reducing the turn-around time and cost. Here, we report a nucleic acid testing-based method aiming to detect and discriminate SARS-CoV-2 mutations by combining RT-RPA and CRISPR-Cas12a detecting assays (RRCd). With a detection limit of 10 copies RNA/reaction, RRCd was validated in 194 clinical samples, showing 89% positive predictive agreement and 100% negative predictive agreement, respectively...
April 15, 2023: Sensors and Actuators. B, Chemical