Lindsay A Beaton-Green, Jessica M Mayenburg, Leonora Marro, Eman Hassan, Sarita Cuadros Sanchez, Riham Darwish, Sylvie Lachapelle, Nadine Adam, Julie J Burtt, Cyndi Van Den Hanenberg, Matthew A Rodrigues, Qi Wang, David J Brenner, Helen C Turner, Ruth Wilkins
The cytokinesis-block micronucleus (CBMN) assay is a well-established method to assess radiation-induced genetic damage in human cells. This assay has been adapted to imaging flow cytometry (IFC), allowing automated analysis of many cells, and eliminating the need to create microscope slides. Furthermore, to improve the efficiency of assay performance, a small-volume method previously developed was employed. Irradiated human blood samples were cultured, stained, and analysed by IFC to produce images of the cells...
August 1, 2023: Cytogenetic and Genome Research