journal
https://read.qxmd.com/read/38532467/high-cell-density-sequential-batch-fermentation-for-enhanced-propionic-acid-production-from-glucose-and-glycerol-glucose-mixture-using-acidipropionibacterium-acidipropionici
#1
JOURNAL ARTICLE
Tarek Dishisha, Mridul Jain, Rajni Hatti-Kaul
BACKGROUND: Propionic acid fermentation from renewable feedstock suffers from low volumetric productivity and final product concentration, which limits the industrial feasibility of the microbial route. High cell density fermentation techniques overcome these limitations. Here, propionic acid (PA) production from glucose and a crude glycerol/glucose mixture was evaluated using Acidipropionibacterium acidipropionici, in high cell density (HCD) batch fermentations with cell recycle. The agro-industrial by-product, heat-treated potato juice, was used as N-source...
March 26, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38528584/potential-use-of-proprotein-convertase-subtilisin-kexin-type-9-pcsk9-inhibition-and-prevention-method-in-viral-infection
#2
REVIEW
Khursheed Muzammil, Mohammad Hosseini Hooshiar, Shirin Varmazyar, Thabit Moath Omar, Manal Morad Karim, Sadeq Aadi, Shaylan Kalavi, Saman Yasamineh
Cellular lipid membranes serve as the primary barrier preventing viral infection of the host cell and provide viruses with a critical initial point of contact. Occasionally, viruses can utilize lipids as viral receptors. Viruses depend significantly on lipid rafts for infection at virtually every stage of their life cycle. The pivotal role that proprotein convertase subtilisin/kexin Type 9 (PCSK9) plays in cholesterol homeostasis and atherosclerosis, primarily by post-transcriptionally regulating hepatic low-density lipoprotein receptor (LDLR) and promoting its lysosomal degradation, has garnered increasing interest...
March 25, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38528536/lyssyl-a-broad-spectrum-phage-endolysin-targeting-staphylococcus-species-and-eradicating-s-aureus-biofilms
#3
JOURNAL ARTICLE
He Liu, Xuemei Wei, Zhefen Wang, Xiaonan Huang, Mengyang Li, Zhen Hu, Kexin Zhang, Qiwen Hu, Huagang Peng, Weilong Shang, Yi Yang, Yuting Wang, Shuguang Lu, Xiancai Rao
BACKGROUND: Staphylococcus aureus and its single or mixed biofilm infections seriously threaten global public health. Phage therapy, which uses active phage particles or phage-derived endolysins, has emerged as a promising alternative strategy to antibiotic treatment. However, high-efficient phage therapeutic regimens have yet to be established. RESULTS: In this study, we used an enrichment procedure to isolate phages against methicillin-resistant S. aureus (MRSA) XN108...
March 25, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38519954/temporal-dynamics-of-stress-response-in-halomonas-elongata-to-nacl-shock-physiological-metabolomic-and-transcriptomic-insights
#4
JOURNAL ARTICLE
Junxiong Yu, Yue Zhang, Hao Liu, Yuxuan Liu, Ali Mohsin, Zebo Liu, Yanning Zheng, Jianmin Xing, Jing Han, Yingping Zhuang, Meijin Guo, Zejian Wang
BACKGROUND: The halophilic bacterium Halomonas elongata is an industrially important strain for ectoine production, with high value and intense research focus. While existing studies primarily delve into the adaptive mechanisms of this bacterium under fixed salt concentrations, there is a notable dearth of attention regarding its response to fluctuating saline environments. Consequently, the stress response of H. elongata to salt shock remains inadequately understood. RESULTS: This study investigated the stress response mechanism of H...
March 23, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38515152/mutagenetic-analysis-of-the-biosynthetic-pathway-of-tetramate-bripiodionen-bearing-3-2h-pyran-2-ylidene-pyrrolidine-2-4-dione-skeleton
#5
JOURNAL ARTICLE
Haixia Zang, Yijia Cheng, Mengjia Li, Lin Zhou, Li-Li Hong, Hai Deng, Hou-Wen Lin, Yongjun Zhou
BACKGROUND: Natural tetramates are a family of hybrid polyketides bearing tetramic acid (pyrrolidine-2,4-dione) moiety exhibiting a broad range of bioactivities. Biosynthesis of tetramates in microorganisms is normally directed by hybrid polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) machineries, which form the tetramic acid ring by recruiting trans- or cis-acting thioesterase-like Dieckmann cyclase in bacteria. There are a group of tetramates with unique skeleton of 3-(2H-pyran-2-ylidene)pyrrolidine-2,4-dione, which remain to be investigated for their biosynthetic logics...
March 21, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38509572/modifications-of-the-5-region-of-the-caspon-tm-tag-s-mrna-further-enhance-soluble-recombinant-protein-production-in-escherichia-coli
#6
JOURNAL ARTICLE
Christoph Köppl, Wolfgang Buchinger, Gerald Striedner, Monika Cserjan-Puschmann
BACKGROUND: Escherichia coli is one of the most commonly used host organisms for the production of biopharmaceuticals, as it allows for cost-efficient and fast recombinant protein expression. However, challenging proteins are often produced with low titres or as inclusion bodies, and the manufacturing process needs to be developed individually for each protein. Recently, we developed the CASPONTM technology, a generic fusion tag-based platform process for high-titer soluble expression including a standardized downstream processing and highly specific enzymatic cleavage of the fusion tag...
March 20, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38493086/engineering-saccharomyces-cerevisiae-for-targeted-hydrolysis-and-fermentation-of-glucuronoxylan-through-crispr-cas9-genome-editing
#7
JOURNAL ARTICLE
Jonas L Ravn, João H C Manfrão-Netto, Jana B Schaubeder, Luca Torello Pianale, Stefan Spirk, Iván F Ciklic, Cecilia Geijer
BACKGROUND: The abundance of glucuronoxylan (GX) in agricultural and forestry residual side streams positions it as a promising feedstock for microbial conversion into valuable compounds. By engineering strains of the widely employed cell factory Saccharomyces cerevisiae with the ability to directly hydrolyze and ferment GX polymers, we can avoid the need for harsh chemical pretreatments and costly enzymatic hydrolysis steps prior to fermentation. However, for an economically viable bioproduction process, the engineered strains must efficiently express and secrete enzymes that act in synergy to hydrolyze the targeted polymers...
March 16, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38486280/rubisco-activity-assays-a-simplified-biochemical-redox-approach-for-in-vitro-quantification-and-an-rna-sensor-approach-for-in-vivo-monitoring
#8
JOURNAL ARTICLE
Muhammad Faisal, Aditya P Sarnaik, Nandini Kannoju, Nima Hajinajaf, Muhammad Javaid Asad, Ryan W Davis, Arul M Varman
BACKGROUND: Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the most abundant soluble protein in nature. Extensive studies have been conducted for improving its activity in photosynthesis through approaches like protein engineering. Concurrently, multiple biochemical and radiolabeling assays have been developed for determining its activity. Although these existing assays yield reliable results, they require addition of multiple external components, rendering them less convenient and expensive...
March 14, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38486239/production-characterization-and-biomedical-potential-of-biosurfactants-produced-by-haloalkaliphilic-archaea-from-wadi-el-natrun-egypt
#9
JOURNAL ARTICLE
Basma T Alghamrawy, Ghada E Hegazy, Soraya A Sabry, Hanan Ghozlan
Extreme halophilic archaea that can live in high saline environments can offer potential applications in different biotechnological fields. This study delves into the fascinating field of halophilic archaea and their ability to produce biosurfactants. Some strains of haloarchaea were isolated from Wadi El-Natrun and were screened for biosurfactants production in a standard basal medium using emulsification index assay. Two strains were chosen as the potential strains for surface tension reduction. They were identified as Natrialba sp...
March 14, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38481305/development-of-hybrid-biomicroparticles-cellulose-exposing-functionalized-fusion-proteins
#10
JOURNAL ARTICLE
Joanna Żebrowska, Piotr Mucha, Maciej Prusinowski, Daria Krefft, Agnieszka Żylicz-Stachula, Milena Deptuła, Aneta Skoniecka, Agata Tymińska, Małgorzata Zawrzykraj, Jacek Zieliński, Michał Pikuła, Piotr M Skowron
BACKGROUND: One of the leading current trends in technology is the miniaturization of devices to the microscale and nanoscale. The highly advanced approaches are based on biological systems, subjected to bioengineering using chemical, enzymatic and recombinant methods. Here we have utilised the biological affinity towards cellulose of the cellulose binding domain (CBD) fused with recombinant proteins. RESULTS: Here we focused on fusions with 'artificial', concatemeric proteins with preprogrammed functions, constructed using DNA FACE™ technology...
March 14, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38481270/the-strain-dependent-cytostatic-activity-of-lactococcus-lactis-on-crc-cell-lines-is-mediated-through-the-release-of-arginine-deiminase
#11
JOURNAL ARTICLE
Rafał Jastrząb, Rafał Tomecki, Aneta Jurkiewicz, Damian Graczyk, Agnieszka K Szczepankowska, Jennifer Mytych, Damian Wolman, Pawel Siedlecki
BACKGROUND: Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, posing a serious public health challenge that necessitates the development of new therapeutics, therapies, and prevention methods. Among the various therapeutic approaches, interventions involving lactic acid bacteria (LAB) as probiotics and postbiotics have emerged as promising candidates for treating and preventing CRC. While human-isolated LAB strains are considered highly favorable, those sourced from environmental reservoirs such as dairy and fermented foods are also being recognized as potential sources for future therapeutics...
March 14, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38481222/improving-furfural-tolerance-in-a-xylose-fermenting-yeast-spathaspora-passalidarum-cmuwf1-2-via-adaptive-laboratory-evolution
#12
JOURNAL ARTICLE
Thanyalak Saengphing, Pachara Sattayawat, Thitisuda Kalawil, Nakarin Suwannarach, Jaturong Kumla, Mamoru Yamada, Watanalai Panbangred, Nadchanok Rodrussamee
BACKGROUND: Spathaspora passalidarum is a yeast with the highly effective capability of fermenting several monosaccharides in lignocellulosic hydrolysates, especially xylose. However, this yeast was shown to be sensitive to furfural released during pretreatment and hydrolysis processes of lignocellulose biomass. We aimed to improve furfural tolerance in a previously isolated S. passalidarum CMUWF1-2, which presented thermotolerance and no detectable glucose repression, via adaptive laboratory evolution (ALE)...
March 13, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38481199/unveiling-the-silver-lining-examining-the-effects-of-biogenic-silver-nanoparticles-on-the-growth-dynamics-of-in-vitro-olive-shoots
#13
JOURNAL ARTICLE
Mohamed S Hasanin, Sayed A M Hassan, A M AbdAllatif, Osama M Darwesh
The current study aimed to evaluate the effects of biogenic silver nanoparticles (AgNPs) on growth behavior and leaf anatomy of in vitro growing shoots of 'Picual' and 'Dolce' olive cultivars. Biosynthesis of AgNPs was carried out using the cell-free filtrate of Fusarium oxysporum. The dimension and shape of the synthesized AgNPs have been analyzed using spectroscopy and topography analysis tools, confirming that the biosynthesis of AgNPs is a crystalline nanostructure with an average particle size of 37 nm...
March 13, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38475853/production-and-bioprocessing-of-taxol-from-aspergillus-niger-an-endophyte-of-encephalartos-whitelockii-with-a-plausible-biosynthetic-stability-antiproliferative-activity-and-cell-cycle-analysis
#14
JOURNAL ARTICLE
Asmaa Gamal, Eman Fikry, Nora Tawfeek, Azza M El-Shafae, Ashraf S A El-Sayed, Maher M El-Domiaty
The biosynthetic potency of Taxol by fungi raises their prospective to be a platform for commercial production of Taxol, nevertheless, the attenuation of its productivity with the fungal storage, is the challenge. Thus, screening for a novel fungal isolate inhabiting ethnopharmacological plants, with a plausible metabolic stability for Taxol production could be one of the most affordable approaches. Aspergillus niger OR414905.1, an endophyte of Encephalartos whitelockii, had the highest Taxol productivity (173...
March 12, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38475794/transcriptome-analysis-reveals-multiple-targets-of-erythritol-related-transcription-factor-euf1-in-unconventional-yeast-yarrowia-lipolytica
#15
JOURNAL ARTICLE
D A Rzechonek, M Szczepańczyk, I Borodina, C Neuvéglise, A M Mirończuk
BACKGROUND: Erythritol is a four-carbon polyol with an unclear role in metabolism of some unconventional yeasts. Its production has been linked to the osmotic stress response, but the mechanism of stress protection remains unclear. Additionally, erythritol can be used as a carbon source. In the yeast Yarrowia lipolytica, its assimilation is activated by the transcription factor Euf1. The study investigates whether this factor can link erythritol to other processes in the cell. RESULTS: The research was performed on two closely related strains of Y...
March 12, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38461254/effective-synthesis-of-high-content-fructooligosaccharides-in-engineered-aspergillus-niger
#16
JOURNAL ARTICLE
Xiufen Wan, Lu Wang, Jingjing Chang, Jing Zhang, Zhiyun Zhang, Kewen Li, Guilian Sun, Caixia Liu, Yaohua Zhong
BACKGROUND: Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS accompanied by the by-product (glucose) generation. This study aims to consume glucose to enhance the content of FOS by heterologously expressing glucose oxidase and peroxidase in engineered A. niger. RESULTS: Glucose oxidase was successfully expressed and co-localized with β-fructofuranosidase in mycelia...
March 9, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38448924/correction-stable-expression-of-hiv-1-mper-extended-epitope-on-the-surface-of-the-recombinant-probiotic-bacteria-escherichia-coli-nissle-1917-using-crispr-cas9
#17
Nathaniel Ninyio, Katharina Schmitt, Gladys Sergon, Charlotta Nilsson, Sören Andersson, Nikolai Scherbak
No abstract text is available yet for this article.
March 6, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38433206/automated-workflow-for-characterization-of-bacteriocin-production-in-natural-producers-lactococcus-lactis-and-latilactobacillus-sakei
#18
JOURNAL ARTICLE
Valentin Steier, Lisa Prigolovkin, Alexander Reiter, Tobias Neddermann, Wolfgang Wiechert, Sebastian J Reich, Christian U Riedel, Marco Oldiges
BACKGROUND: Lactic acid bacteria are commonly used as protective starter cultures in food products. Among their beneficial effects is the production of ribosomally synthesized peptides termed bacteriocins that kill or inhibit food-spoiling bacteria and pathogens, e.g., members of the Listeria species. As new bacteriocins and producer strains are being discovered rapidly, modern automated methods for strain evaluation and bioprocess development are required to accelerate screening and development processes...
March 3, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38431598/enhancement-of-cellulolytic-enzyme-production-from-intrageneric-protoplast-fusion-of-aspergillus-species-and-evaluating-the-hydrolysate-scavenging-activity
#19
JOURNAL ARTICLE
Doaa A Goda, Huda M Shakam, Mai E Metwally, Hager A Abdelrasoul, Mohamed M Yacout
BACKGROUND: Lignocellulosic biomass provides a great starting point for the production of energy, chemicals, and fuels. The major component of lignocellulosic biomass is cellulose, the employment of highly effective enzymatic cocktails, which can be produced by a variety of microorganisms including species of the genus Aspergillus, is necessary for its utilization in a more productive manner. In this regard, molecular biology techniques should be utilized to promote the economics of enzyme production, whereas strategies like protoplast fusion could be employed to improve the efficacy of the hydrolytic process...
March 2, 2024: Microbial Cell Factories
https://read.qxmd.com/read/38429691/engineering-escherichia-coli-for-increased-und-p-availability-leads-to-material-improvements-in-glycan-expression-technology
#20
JOURNAL ARTICLE
Emily J Kay, Manoj K Dooda, Joseph C Bryant, Amanda J Reid, Brendan W Wren, Jerry M Troutman, Matthew A Jorgenson
BACKGROUND: Bacterial surface glycans are assembled by glycosyltransferases (GTs) that transfer sugar monomers to long-chained lipid carriers. Most bacteria employ the 55-carbon chain undecaprenyl phosphate (Und-P) to scaffold glycan assembly. The amount of Und-P available for glycan synthesis is thought to be limited by the rate of Und-P synthesis and by competition for Und-P between phosphoglycosyl transferases (PGTs) and GTs that prime glycan assembly (which we collectively refer to as PGT/GTs)...
March 1, 2024: Microbial Cell Factories
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