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Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

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https://read.qxmd.com/read/31103944/improved-folate-monoglutamate-extraction-and-application-to-folate-quantification-from-wild-lentil-seeds-by-ultra-performance-liquid-chromatography-selective-reaction-monitoring-mass-spectrometry
#1
Haixia Zhang, Ambuj B Jha, Devini De Silva, Randy W Purves, Thomas D Warkentin, Albert Vandenberg
Folates are important micronutrients in lentils (Lens culinaris Medik.). In this work, the folate extraction workflow in ascorbate-containing buffer was optimized and validated, and the concentrations of eight folate monoglutamates in cultivated and six wild lentil species, grown under field or greenhouse conditions, were quantified by ultra-performance liquid chromatography and mass spectrometry (UPLC-MS). In general wild lentil species had higher folate concentrations than cultivated genotypes. Lens tomentosus had the highest folate concentration with median values of 439...
May 8, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31082683/validation-of-a-sensitive-simultaneous-lc-ms-ms-method-for-the-quantification-of-novel-anti-cancer-thiazolidinedione-and-quinazolin-4-one-derivatives-in-rat-plasma-and-its-application-in-a-rat-pharmacokinetic-study
#2
Shankar Sengottuvelan, Ritika Uppal, Suneetha Vuppu
Thiazolidinediones and quinazolin-4-ones compounds, previously known for their activity against Type 2 diabetes and antifungal activity respectively, are currently being investigated for their anti-cancer activity. The determination of pharmacokinetic parameters for these two classes of compounds using a simultaneous chromatographic method with a low detection limit is a challenge. In this study, a highly sensitive and simultaneous LC-MS/MS-based bioanalytical method was developed and validated in rat plasma for the estimation of four novel anti-cancer compounds, BIT-15-67 and BNT-11, belonging to the Thiazolidinedione class, and BNUA-108 and BNUA-48, from the quinazolin-4-one class...
May 7, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31100605/rapid-screening-of-nine-unradiolabeled-candidate-compounds-as-pet-brain-imaging-agents-using-cassette-wave-microdosing-and-lc-ms-ms
#3
Mingyue Sun, Hao Xiao, Haiyan Hong, Aili Zhang, Yan Zhang, Yajing Liu, Lin Zhu, Hank F Kung, Jinping Qiao
The R&D of PET imaging agents is a complex system engineering, simplifying screening steps and increasing screening efficiency have become popular issues. The purpose of this study is to develop a new screening procedure using cassette-wave microdosing and LC-MS/MS to enhance the screening throughput of unradiolabeled candidate compounds as PET imaging agents. Nine compounds were divided into 3 sets and made into 3 cassettes. Fifteen rats were randomly divided into 3 groups, and every animal received three intravenous bolus injections at three different time points; the doses were at microdose levels...
May 6, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31082684/a-quantitative-method-to-detect-human-exposure-to-sulfur-and-nitrogen-mustards-via-protein-adducts
#4
Brooke G Pantazides, Jennifer Quiñones-González, Danisha M Rivera Nazario, Brian S Crow, Jonas W Perez, Thomas A Blake, Rudolph C Johnson
Sulfur and nitrogen mustards are internationally banned vesicants listed as Schedule 1 chemical agents in the Chemical Weapons Convention. These compounds are highly reactive electrophiles that form stable adducts to a variety of available amino acid residues on proteins upon exposure. We present a quantitative exposure assay that simultaneously measures agent specific protein adducts to cysteine for sulfur mustard (HD) and three nitrogen mustards (HN1, HN2, and HN3). Proteinase K was added to a serum or plasma sample to digest protein adducts and form the target analyte, the blister agent bound to the tripeptide cysteine-proline-phenylalanine (CPF)...
May 5, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31078128/cupric-ion-functionalized-polydopamine-coated-magnetic-microspheres-as-solid-phase-adsorbent-for-the-extraction-of-purines-in-plasma
#5
Dong-Dong Zhou, Qian Zhang, Hao Zhang, Yin-Zhen Wang, Feng-Qing Yang, Sheng-Peng Wang, Yi-Tao Wang
In this paper, Cu2+ functionalized Fe3 O4 @polydopamine core-shell (Fe3 O4 @PDA@Cu2+ ) magnetic microspheres were prepared by the chelation between Cu2+ and catechol of polydopamine surface. The synthetic magnetic adsorbent was characterized by Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, vibrating sample magnetometry, thermogravimetric analysis, scanning electron microscope and transmission electron microscopy. Four purines include guanine, adenine, hypoxanthine and xanthine were selected as model compounds to evaluate the applicability of this adsorbent...
May 5, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31082718/stability-kinetics-of-fusidic-acid-development-and-validation-of-stability-indicating-analytical-method-by-employing-analytical-quality-by-design-approach-in-medicinal-product-s
#6
Gajanand Sharma, Kanika Thakur, Kaisar Raza, O P Katare
A simple, rapid, accurate, reproducible and sensitive reverse phase HPLC method for the estimation of fusidic acid (FA) by means of Analytical Quality by Design (AQbD) was the aim of the present study. Initially, the vital pre-requisites for AQbD like analytical method target profile and critical analytical attributes (CAAs) like theoretical plates, tailing factor and percent assay were defined. An octadecyl silyl silica C18 column with a packing size of 5 μm was employed and the detection was performed at 235 nm using UV-detector...
May 4, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31079009/optimization-of-protein-entrapment-in-affinity-microcolumns-using-hydrazide-activated-silica-and-glycogen-as-a-capping-agent
#7
John Vargas-Badilla, Saumen Poddar, Shiden Azaria, Chenhua Zhang, David S Hage
Several approaches were compared for the entrapment of proteins within hydrazide-activated silica for use in affinity microcolumns and high performance affinity chromatography. Human serum albumin (HSA) and concanavalin A (Con A) were used as model proteins for this work. Items considered in this study included the role played by the solution volume, amount of added protein, and use of slurry vs. on-column entrapment on the levels of solute retention and extent of protein immobilization that could be obtained by means of entrapment...
May 4, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31078127/lc-electrolyte-switch-in-a-contiguous-time-segments-to-analyze-multi-components-simultaneous-determination-of-phenolic-acids-and-iridoids-in-rat-plasma-after-inhalation-administration-of-reduning-aerosol
#8
Wei Yang, Shuyao Wang, Guangping Zhang, Siyang Wu, Tengfei Chen, Qiutao Wang, Mengmeng Wei, Wenhao Cheng, Yang Gao, Yingfei Li, Zuguang Ye
The optimization of electrolytes, kinds and concentrations, in mobile phase for multiple constituents analyzing using high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS) was usually compromised to ensure good LC separation of partial components. However, the compromised electrolytes could lead to ionization suppression of some of the analytes. To solve the compromise of electrolytes within various components, taking phenolic acids and iridoids as a case, we used electrolyte switch in contiguous running time segments of UPLC-ESI-MS/MS to ensure chromatographic separation of chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid and improve the response of geniposide...
May 2, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31071580/oxidized-glycerophosphatidylcholines-in-diabetes-through-non-targeted-metabolomics-their-annotation-and-biological-meaning
#9
Joanna Godzien, Bartlomiej Kalaska, Edyta Adamska-Patruno, Jitka Siroka, Michal Ciborowski, Adam Kretowski, Coral Barbas
Lipid oxidation is one of the most important processes occurring in living cells and has been investigated through stable end-products. Currently, new insights into many physiological and pathophysiological processes provide a measurement of the first products of oxidation, e.g., oxidized glycerophosphatidylcholines (oxGPCs). Here, we evaluate the capacity of untargeted global metabolomics to measure oxGPCs in serum samples. This evaluation covered analytical reproducibility and data quality as well as the ability to capture metabolic alterations in diverse conditions...
April 29, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31063954/determination-of-amphetamine-type-stimulants-in-urine-samples-using-microextraction-by-packed-sorbent-and-gas-chromatography-mass-spectrometry
#10
Sara Malaca, Tiago Rosado, José Restolho, Jesus M Rodilla, Pedro M M Rocha, Lúcia Silva, Cláudia Margalho, Mário Barroso, Eugenia Gallardo
The aim of this work was the development, optimization and full validation of a method applying microextraction by packed sorbent (MEPS) coupled to gas chromatography-mass spectrometry (GC-MS) to determine amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxyethylmethamphetamine (MDMA), 3,4-methylenedioxy-N-methyl-α-ethylfenilethylamine (MBDB), and 3,4-methylenedioxy-N-ethylamphetamine (MDE) in urine samples. Using 200 μL of sample, the MEPS procedure was optimized concerning type of sorbent, sample dilution, number of strokes, activation of the ion exchange mechanism and composition of both washing and elution solvents...
April 29, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31071582/development-and-application-of-time-staggered-mass-staggered-globally-optimized-targeted-mass-spectrometry
#11
Fanyi Zhong, Mengyang Xu, Jiangjiang Zhu
The emerging requests for handling complex samples in system biology studies highlighted the need to expand the metabolite coverage in metabolomics analysis and to take advantage of the quantitative and targeted assays. Here, we developed a novel workflow to integrate time staggered or mass staggered scan methods with globally optimized targeted-mass spectrometry (GOT-MS), to enable broad metabolites coverage with better stability, repeatability, and quantitative capability. To establish these methods, two scheduled selected reaction monitoring (SRM) methods, time staggered and mass staggered approaches, were configured to achieve optimal sensitivity and scan speed and were combined with the GOT-MS strategy...
April 28, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31060022/simultaneous-profiling-of-vitamin-d-metabolites-in-serum-by-supercritical-fluid-chromatography-tandem-mass-spectrometry-sfc-ms-ms
#12
Ting-Ting Liu, Ling-Zhi Cheong, Qing-Qing Man, Xin Zheng, Jian Zhang, Shuang Song
Simultaneous profiling of serum vitamin D (VD) metabolites with similar structures is a big challenge. Thus, we developed and validated a SFC-MS/MS method, which is capable of eluting hydrophobic molecules, for quantification of VD2 /VD3 , 25-OH VD2 /VD3 , 3-epi-25-OH VD2 /VD3 , 1,25-(OH)2 VD2 /VD3 and 24,25-(OH)2 VD2 /VD3 . VD metabolites were extracted from human serum using acetonitrile solvent. Column stationary phase, elution gradients, flow rate, column temperature, ion-source type and buffer system in post-column make-up solvent were optimized...
April 27, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31071581/a-review-on-native-and-denaturing-purification-methods-for-non-coding-rna-ncrna
#13
REVIEW
Fariha Kanwal, Changrui Lu
Recently, non-coding RNA (ncRNA) became the centerpiece of human genome research. Modern ncRNA-based research has revolutionized disease diagnosis and therapeutics. However, decoding structural/functional information of ncRNA requires large amount of pure RNA, and hence effective RNA preparation and purification protocols. This review focuses on purification schemes of synthetic oligonucleotides, particularly liquid chromatographic (LC) techniques as improved alternatives to urea-polyacrylamide gel electrophoresis (urea-PAGE) purification...
April 26, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31063953/glycan-analysis-for-protein-therapeutics
#14
REVIEW
Xiangkun Yang, Michael G Bartlett
Glycosylation can be a critical quality attribute for protein therapeutics due to its extensive impact on product safety and efficacy. Glycan characterization is important in the process of protein drug development, from early stage candidate selection to late stage regulatory submission. It is also an indispensable part in the evaluation of biosimilarity. This review discusses the effects of glycosylation on the stability and activity of protein therapeutics, regulatory considerations corresponding to manufacturing and structural characterization of glycosylated protein therapeutics, and focuses on mass spectrometry compatible separation methods for glycan characterization of protein therapeutics...
April 26, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31060023/identification-and-quantification-of-dimethyl-acetals-from-plasmalogenic-lipids-in-lamb-intramuscular-fat-under-different-derivatization-procedures
#15
P Gómez-Cortés, V Rodríguez-Pino, A L Martínez Marín, M A de la Fuente
Meat lipids are mostly comprised by triacylglycerols, but small amounts of plasmalogens are also present in intramuscular fat. The purpose of this study was to evaluate the effect of lipid derivatization on the presence of dimethyl acetal (DMA) molecules from plasmalogenic lipids in intramuscular fat samples. Three different methods of methylation were assayed. Acid-catalyzed methanolysis using HCl, the traditional procedure to derivatize meat lipids, was compared to two base-catalyzed methanolysis based on the ISO International standard procedure using either KOH and/or NaOCH3 which, apparently, are only able to methylate fatty acids from triacylglycerols...
April 26, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31055190/a-liquid-chromatography-tandem-mass-spectrometry-method-for-simultaneous-determination-of-simeprevir-daclatasvir-sofosbuvir-and-gs-331007-applied-to-a-retrospective-clinical-pharmacological-study
#16
Davide Ferrari, Sabrina Bagaglio, Michele Raso, Laura Galli, Simone Premaschi, Emanuela Messina, Giulia Morsica, Massimo Locatelli, Caterina Uberti-Foppa, Hamid Hasson
A highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of daclatasvir (DCV), simeprevir (SMV), sofosbuvir (SOF), and its major metabolite GS-331007 in human plasma using stable-isotope-labeled (SIL) analogs as internal standards (IS) to minimize a possible matrix effect. Liquid-liquid extraction (LLE) of the analytes and IS from human plasma was performed using a commercial extraction kit requiring low sample volume (50 μL)...
April 26, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31054451/fabric-phase-sorptive-extraction-for-the-isolation-of-five-common-antidepressants-from-human-urine-prior-to-hplc-dad-analysis
#17
Artemis Lioupi, Abuzar Kabir, Kenneth G Furton, Victoria Samanidou
An innovative fabric phase sorptive extraction-high performance liquid chromatography-diode array detection (FPSE-HPLC-DAD) method is developed and validated herein for the simultaneous extraction and analysis of five common antidepressants (venlafaxine, paroxetine, fluoxetine, amitriptyline, clomipramine) in human urine samples. Separation was obtained using a reverse phase column LiChroCART-LiChrospher®100 RP-8e (5 μm, 250 × 4 mm) with diode array detection monitoring at 235 nm. Gradient elution was applied at a flow rate of 1...
April 25, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31054450/chromatographic-separation-of-the-isobaric-compounds-cyclopropylfentanyl-crotonylfentanyl-methacrylfentanyl-and-para-methylacrylfentanyl-for-specific-confirmation-by-lc-ms-ms
#18
Juseon Lee, Alex J Krotulski, Melissa F Fogarty, Donna M Papsun, Barry K Logan
Cyclopropylfentanyl has been encountered by law enforcement and public health officials beginning in mid-2017 and has been associated with numerous overdoses and fatalities. The detection and identification of cyclopropylfentanyl has become more challenging with the subsequent emergence of multiple structural isomeric fentanyl species, some of which have been detected in seized drug casework. These include crotonylfentanyl, methacrylfentanyl, para-methylacrylfentanyl, and ortho-methylacrylfentanyl; all of which have the same exact mass and similar fragmentation patterns...
April 25, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31054449/urinary-metabolomic-profiling-for-noninvasive-diagnosis-of-acute-t-cell-mediated-rejection-after-kidney-transplantation
#19
Sun-Young Kim, Bo Kyung Kim, Mi-Ri Gwon, Sook Jin Seong, Boram Ohk, Woo Youl Kang, Hae Won Lee, Hee-Yeon Jung, Jang-Hee Cho, Byung Ha Chung, Sang-Ho Lee, Yeong Hoon Kim, Young-Ran Yoon, Chan-Duck Kim, Seungil Cho
To improve early renal allograft function, it is important to develop a noninvasive diagnostic method for acute T cell-mediated rejection (TCMR). This study aims to explore potential noninvasive urinary biomarkers to screen for acute TCMR in kidney transplant recipients (KTRs) using untargeted metabolomic profiling. Urinary metabolites, collected from KTRs with stable graft function (STA) or acute TCMR episodes, were analyzed using liquid chromatography-mass spectrometry (LC-MS). Multivariate statistical analyses were performed to discriminate differences in urinary metabolites between the two groups...
April 24, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/31071583/re-certification-of-hydroxyvitamin-d-standards-by-isotope-pattern-deconvolution
#20
J Pitarch-Motellón, A F Roig-Navarro, C Le Goff, E Cavalier, N Fabregat-Cabello
BACKGROUND: Vitamin D testing in analytical clinical laboratories has been experiencing a rapid increase of demand over the last years, as it plays a key role in several disorders. Due to the narrow ranges of medical significance regarding its concentration levels in human serum, accurate and precise determinations of vitamin D metabolites are required. METHODS: We present an isotope dilution mass spectrometry quantification method for the re-certification of routine commercial standards used in method validation steps, isotope pattern deconvolution (IPD) based on LC-MS/MS...
April 22, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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