Amr R Salem, W Bart Bryant, Jaser Doja, Susan H Griffin, Xiaofan Shi, Weihong Han, Yunchao Su, Alexander D Verin, Joseph M Miano
CRISPR editing involves double-strand breaks in DNA with attending insertions/deletions (indels) that may result in embryonic lethality in mice. The prime editing (PE) platform uses a prime editing guide RNA (pegRNA) and a Cas9 nickase fused to a modified reverse transcriptase to precisely introduce nucleotide substitutions or small indels without the unintended editing associated with DNA double-strand breaks. Recently, engineered pegRNAs (epegRNAs), with a 3'-extension that shields the primer-binding site of the pegRNA from nucleolytic attack, demonstrated superior activity over conventional pegRNAs in cultured cells...
December 27, 2023: Vascular Pharmacology