journal
https://read.qxmd.com/read/38441218/microphysiological-pancreas-on-chip-platform-with-integrated-sensors-to-model-endocrine-function-and-metabolism
#21
JOURNAL ARTICLE
Katharina Schlünder, Madalena Cipriano, Aline Zbinden, Stefanie Fuchs, Torsten Mayr, Katja Schenke-Layland, Peter Loskill
Pancreatic in vitro research is of major importance to advance mechanistic understanding and development of treatment options for diseases such as diabetes mellitus. We present a thermoplastic-based microphysiological system aiming to model the complex microphysiological structure and function of the endocrine pancreas with concurrent real-time read-out capabilities. The specifically tailored platform enables self-guided trapping of single islets at defined locations: β-cells are assembled to pseudo-islets and injected into the tissue chamber using hydrostatic pressure-driven flow...
March 5, 2024: Lab on a Chip
https://read.qxmd.com/read/38436397/microfluidic-impedance-cytometry-with-flat-end-cylindrical-electrodes-for-accurate-and-fast-analysis-of-marine-microalgae
#22
JOURNAL ARTICLE
Xiaoming Chen, Mo Shen, Shun Liu, Chungang Wu, Liangliang Sun, Zhipeng Song, Jishun Shi, Yulong Yuan, Yong Zhao
Marine microalgae play an increasingly significant role in addressing the issues of environmental monitoring and disease treatment, making the analysis of marine microalgae at the single-cell level an essential technique. For this, we put forward accurate and fast microfluidic impedance cytometry to analyze microalgal cells by assembling two cylindrical electrodes and microchannels to form a three-dimensional detection zone. Firstly, we established a mathematical model of microalgal cell detection based on Maxwell's mixture theory and numerically investigated the effects of the electrode gap, microalgal positions, and ion concentrations of the solution on detection to optimize detection conditions...
March 4, 2024: Lab on a Chip
https://read.qxmd.com/read/38436394/tonicity-induced-cargo-loading-into-extracellular-vesicles
#23
JOURNAL ARTICLE
Chaeeun Lee, Sumit Kumar, Juhee Park, Yongjun Choi, Elizabeth Maria Clarissa, Yoon-Kyoung Cho
The current challenge in using extracellular vesicles (EVs) as drug delivery vehicles is to precisely control their membrane permeability, specifically in the ability to switch between permeable and impermeable states without compromising their integrity and functionality. Here, we introduce a rapid, efficient, and gentle loading method for EVs based on tonicity control (TC) using a lab-on-a-disc platform. In this technique, a hypotonic solution was used for temporarily permeabilizing a membrane ("on" state), allowing the influx of molecules into EVs...
March 4, 2024: Lab on a Chip
https://read.qxmd.com/read/38436364/droplet-microfluidic-system-for-high-throughput-and-passive-selection-of-bacteria-producing-biosurfactants
#24
JOURNAL ARTICLE
Klaudia Staskiewicz, Maria Dabrowska-Zawada, Lukasz Kozon, Zofia Olszewska, Lukasz Drewniak, Tomasz S Kaminski
Traditional methods for the enrichment of microorganisms rely on growth in a selective liquid medium or on an agar plate, followed by tedious characterization. Droplet microfluidic techniques have been recently used to cultivate microorganisms and preserve enriched bacterial taxonomic diversity. However, new methods are needed to select droplets comprising not only growing microorganisms but also those exhibiting specific properties, such as the production of value-added compounds. We describe here a droplet microfluidic screening technique for the functional selection of biosurfactant-producing microorganisms, which are of great interest in the bioremediation and biotechnology industries...
March 4, 2024: Lab on a Chip
https://read.qxmd.com/read/38426311/capturing-of-extracellular-vesicles-derived-from-single-cells-of-escherichia-coli
#25
JOURNAL ARTICLE
Fumiaki Yokoyama, André Kling, Petra S Dittrich
Bacteria secrete extracellular vesicles (EVs), also referred to as bacterial membrane vesicles, which carry, among other compounds, lipids, nucleic acids and virulence factors. Recent studies highlight the role of EVs in the emergence of antibiotic resistance, e.g. as carrier and absorbent particles of the drug to protect the cells, or as a pathway to disseminate resistance elements. In this study, we are interested in characterizing the secretion of EVs at the single bacterial level to ultimately understand how cells respond to antibiotic treatment...
March 1, 2024: Lab on a Chip
https://read.qxmd.com/read/38416560/-amf-sporechip-provides-new-insights-into-arbuscular-mycorrhizal-fungal-asymbiotic-hyphal-growth-dynamics-at-the-cellular-level
#26
JOURNAL ARTICLE
Felix Richter, Maryline Calonne-Salmon, Marcel G A van der Heijden, Stéphane Declerck, Claire E Stanley
Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with the majority of land plants and deliver a wide range of soil-based ecosystem services. Due to their conspicuous belowground lifestyle in a dark environment surrounded by soil particles, much is still to be learned about the influence of environmental ( i.e. , physical) cues on spore germination, hyphal morphogenesis and anastomosis/hyphal healing mechanisms. To fill existing gaps in AMF knowledge, we developed a new microfluidic platform - the AMF-SporeChip - to visualise the foraging behaviour of germinating Rhizophagus and Gigaspora spores and confront asymbiotic hyphae with physical obstacles...
February 28, 2024: Lab on a Chip
https://read.qxmd.com/read/38414410/comparison-between-dynamic-versus-static-models-and-real-time-monitoring-of-neuronal-dysfunction-in-an-amyloid-%C3%AE-induced-neuronal-toxic-model-on-a-chip-platform
#27
JOURNAL ARTICLE
Chu-Chun Liang, Po-Yen Chen, Nien-Che Liu, I-Chi Lee
Microfluidics-based organs-on-a-chip offer a promising method for dynamic and 3-dimensional (3D) cell culture to evaluate the cell behaviors within the biomimetic environment. The purpose of this study was to establish neural network connections in a 3D neural stem cell (NSC)-based system with an interstitial level of flow for simulating the brain microenvironment toward a dynamic amyloid-β (Aβ) induced neuronal toxic model on a chip and to compare the biological effects and neurite dysfunction between static and dynamic systems...
February 28, 2024: Lab on a Chip
https://read.qxmd.com/read/38411270/lab-on-skin-real-time-metabolite-monitoring-with-polyphenol-film-based-subdermal-wearable-patches
#28
JOURNAL ARTICLE
Georgeta Vulpe, Guoyi Liu, Sam Oakley, Guanghao Yang, Arjun Ajith Mohan, Mark Waldron, Sanjiv Sharma
The advent of digital technologies has spurred the development of wearable sensing devices marking a significant shift in obtaining real-time physiological information. The principal objective is to transition from blood-centric monitoring to minimally invasive modalities, which will enable movement from specialised settings to more accessible environments such as the practices of general practitioners or even home settings. While subcutaneously implanted continuous monitoring devices have demonstrated this transition, detection of analytes from sample matrices like skin interstitial fluid (ISF), is a frontier that offers attractive minimally invasive routes for detection of biomarkers...
February 27, 2024: Lab on a Chip
https://read.qxmd.com/read/38410967/deciphering-fibroblast-induced-drug-resistance-in-non-small-cell-lung-carcinoma-through-patient-derived-organoids-in-agarose-microwells
#29
JOURNAL ARTICLE
Qiyue Luan, Ines Pulido, Angelique Isagirre, Julian Carretero, Jian Zhou, Takeshi Shimamura, Ian Papautsky
Patient-derived organoids (PDOs) serve as invaluable 3D tumor models, retaining the histological complexity and genetic heterogeneity found in primary tumors. However, the limitation of small sample volumes and the lack of tailored platforms have hindered the research using PDOs. Within the tumor microenvironment, cancer-associated fibroblasts play a pivotal role in influencing drug sensitivity. In this study, we introduce an agarose microwell platform designed for PDO-based tumor and tumor microenvironment models, enabling rapid drug screening and resistance studies with small sample volumes...
February 27, 2024: Lab on a Chip
https://read.qxmd.com/read/38407354/confining-calcium-oxalate-crystal-growth-in-a-carbonated-apatite-coated-microfluidic-channel-to-better-understand-the-role-of-randall-s-plaque-in-kidney-stone-formation
#30
JOURNAL ARTICLE
Samantha Bourg, Karol Rakotozandriny, Ivan T Lucas, Emmanuel Letavernier, Christian Bonhomme, Florence Babonneau, Ali Abou-Hassan
Effective prevention of recurrent kidney stone disease requires the understanding of the mechanisms of its formation. Numerous in vivo observations have demonstrated that a large number of pathological calcium oxalate kidney stones develop on an apatitic calcium phosphate deposit, known as Randall's plaque. In an attempt to understand the role of the inorganic hydroxyapatite phase in the formation and habits of calcium oxalates, we confined their growth under dynamic physicochemical and flow conditions in a reversible microfluidic channel coated with hydroxyapatite...
February 26, 2024: Lab on a Chip
https://read.qxmd.com/read/38390638/dynamic-behavior-of-floating-magnetic-liquid-marbles-under-steady-and-pulse-width-modulated-magnetic-fields
#31
JOURNAL ARTICLE
Hossein Dayyani, Alireza Mohseni, Mohamad Ali Bijarchi
Liquid marbles show promising potential for digital microfluidic devices due to their lower friction with the platform surface than non-covered droplets. In this study, the manipulation of a biocompatible magnetic liquid marble with a magnetic shell (LMMS) is experimentally studied. The movement of the floating LMMS on the water surface, which is actuated by DC and pulse width modulation (PWM) magnetic fields, is investigated under the influence of various parameters, including the LMMS volume, the initial distance of the LMMS from the magnetic coil tip, the magnetic coil current, the PWM frequency and its duty cycle...
February 23, 2024: Lab on a Chip
https://read.qxmd.com/read/38385159/multifunctional-cardiac-microphysiological-system-based-on-transparent-ito-electrodes-for-simultaneous-optical-measurement-and-electrical-signal-monitoring
#32
JOURNAL ARTICLE
Zhangjie Li, Kai Niu, Chenyang Zhou, Feifan Wang, Kangyi Lu, Yijun Liu, Lian Xuan, Xiaolin Wang
Drug-induced cardiotoxicity is a significant contributor to drug recalls, primarily attributed to limitations in existing drug screening platforms. Traditional heart-on-a-chip platforms often employ metallic electrodes to record cardiomyocyte electrical signals. However, this approach hinders direct cardiomyocyte morphology observation and typically yields limited functionality. Consequently, this limitation may lead to an incomplete understanding of cardiomyocyte characteristics. To address these challenges, we introduce a multifunctional cardiac microphysiological system featuring transparent indium tin oxide electrodes...
February 22, 2024: Lab on a Chip
https://read.qxmd.com/read/38373026/wearable-intelligent-sweat-platform-for-sers-ai-diagnosis-of-gout
#33
JOURNAL ARTICLE
Zhaoxian Chen, Wei Wang, Hao Tian, Wenrou Yu, Yu Niu, Xueli Zheng, Shihong Liu, Li Wang, Yingzhou Huang
For the past few years, sweat analysis for health monitoring has attracted increasing attention benefiting from wearable technology. In related research, the sensitive detection of uric acid (UA) in sweat with complex composition based on surface-enhanced Raman spectroscopy (SERS) for the diagnosis of gout is still a significant challenge. Herein, we report a visualized and intelligent wearable sweat platform for SERS detection of UA in sweat. In this wearable platform, the spiral channel consisted of colorimetric paper with Ag nanowires (AgNWs) that could capture sweat for SERS measurement...
February 19, 2024: Lab on a Chip
https://read.qxmd.com/read/38372633/a-microfluidic-chip-for-sustained-oxygen-gradient-formation-in-the-intestine-ex-vivo
#34
JOURNAL ARTICLE
Lauren M Delong, Colby E Witt, Madison Pennell, Ashley E Ross
The oxygen gradient across the intestine influences intestinal physiology and the microbial environment of the microbiome. The microbiome releases metabolites that communicate with enterochromaffin cells, neuronal cells, and resident immune cells to facilitate the bidirectional communication across the gut-brain axis. Measuring communication between various cell types within the intestine could provide essential information about key regulators of gut and brain health; however, the microbial environment of the intestine is heavily dependent on the physiological oxygen gradient that exists across the intestinal wall...
February 19, 2024: Lab on a Chip
https://read.qxmd.com/read/38372578/a-miniprotein-receptor-electrochemical-biosensor-chip-based-on-quantum-dots
#35
JOURNAL ARTICLE
Yunong Zhao, Juan Han, Jing Huang, Qing Huang, Yanbing Tao, Ruiqin Gu, Hua-Yao Li, Yang Zhang, Houjin Zhang, Huan Liu
Recently protein binders have emerged as a promising substitute for antibodies due to their high specificity and low cost. Herein, we demonstrate an electrochemical biosensor chip through the electronic labelling strategy using lead sulfide (PbS) colloidal quantum dots (CQDs) and the unnatural SARS-CoV-2 spike miniprotein receptor LCB. The unnatural receptor can be utilized as a molecular probe for the construction of CQD-based electrochemical biosensor chips, through which the specific binding of LCB and the spike protein is transduced to sensor electrical signals...
February 19, 2024: Lab on a Chip
https://read.qxmd.com/read/38372397/test-strip-coupled-cas12a-assisted-signal-amplification-strategy-for-sensitive-detection-of-uracil-dna-glycosylase
#36
JOURNAL ARTICLE
Bin Guo, Chong Hu, Zeping Yang, Chu Tang, Chuanxian Zhang, Fu Wang
Uracil-DNA glycosylase (UDG) is a base excision repair (BER) enzyme, which catalyzes the hydrolysis of uracil bases in DNA chains that contain uracil and N -glycosidic bonds of the sugar phosphate backbone. The expression of UDG enzyme is associated with a variety of genetic diseases including cancers. Hence, the identification of UDG activity in cellular processes holds immense importance for clinical investigation and diagnosis. In this study, we employed Cas12a protein and enzyme-assisted cycle amplification technology with a test strip to establish a precise platform for the detection of UDG enzyme...
February 19, 2024: Lab on a Chip
https://read.qxmd.com/read/38372394/a-system-for-fluid-pumping-by-liquid-metal-multi-droplets
#37
JOURNAL ARTICLE
Liyu Dai, Xiaomin Wu, Huimin Hou, Zhifeng Hu, Yukai Lin, Zhiping Yuan
The transportation and control of microfluidics have an important influence on the fields of biology, chemistry, and medicine. Pump systems based on the electrocapillary effect and room-temperature liquid metal droplets have attracted extensive attention. Flow rate is an important parameter that reflects the delivery performance of the pump systems. In the systems of previous studies, cylindrical structures are mostly used to constrain the droplet. The analysis and quantitative description of the influence of voltage frequency, alternating voltage, direct current voltage bias, and solution concentration on the flow rate are not yet comprehensive...
February 19, 2024: Lab on a Chip
https://read.qxmd.com/read/38372324/microfluidic-systems-for-infectious-disease-diagnostics
#38
REVIEW
Thomas Lehnert, Martin A M Gijs
Microorganisms, encompassing both uni- and multicellular entities, exhibit remarkable diversity as omnipresent life forms in nature. They play a pivotal role by supplying essential components for sustaining biological processes across diverse ecosystems, including higher host organisms. The complex interactions within the human gut microbiota are crucial for metabolic functions, immune responses, and biochemical signalling, particularly through the gut-brain axis. Viruses also play important roles in biological processes, for example by increasing genetic diversity through horizontal gene transfer when replicating inside living cells...
February 19, 2024: Lab on a Chip
https://read.qxmd.com/read/38372151/from-animal-testing-to-in-vitro-systems-advancing-standardization-in-microphysiological-systems
#39
REVIEW
Darwin R Reyes, Mandy B Esch, Lorna Ewart, Rohollah Nasiri, Anna Herland, Kyung Sung, Monica Piergiovanni, Carolina Lucchesi, James T Shoemaker, Jelena Vukasinovic, Hiroki Nakae, James Hickman, Kapil Pant, Anne Taylor, Niki Heinz, Nureddin Ashammakhi
Limitations with cell cultures and experimental animal-based studies have had the scientific and industrial communities searching for new approaches that can provide reliable human models for applications such as drug development, toxicological assessment, and in vitro pre-clinical evaluation. This has resulted in the development of microfluidic-based cultures that may better represent organs and organ systems in vivo than conventional monolayer cell cultures. Although there is considerable interest from industry and regulatory bodies in this technology, several challenges need to be addressed for it to reach its full potential...
February 19, 2024: Lab on a Chip
https://read.qxmd.com/read/38363157/immune-cells-and-inflammatory-mediators-cause-endothelial-dysfunction-in-a-vascular-microphysiological-system
#40
JOURNAL ARTICLE
Aishwarya Rengarajan, Hannah E Goldblatt, David J Beebe, María Virumbrales-Muñoz, Derek S Boeldt
Functional assessment of endothelium serves as an important indicator of vascular health and is compromised in vascular disorders including hypertension, atherosclerosis, and preeclampsia. Endothelial dysfunction in these cases is linked to dysregulation of the immune system involving both changes to immune cells and increased secretion of inflammatory cytokines. Herein, we utilize a well-established microfluidic device to generate a 3-dimensional vascular microphysiological system (MPS) consisting of a tubular blood vessel lined with human umbilical vein endothelial cells (HUVECs) to evaluate endothelial function measured via endothelial permeability and Ca2+ signaling...
February 16, 2024: Lab on a Chip
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