Lia R Serrano, Trenton M Peters-Clarke, Tabiwang N Arrey, Nicolaie E Damoc, Margaret Lea Robinson, Noah M Lancaster, Evgenia Shishkova, Corinne Moss, Anna Pashkova, Pavel Sinitcyn, Dain R Brademan, Scott T Quarmby, Amelia C Peterson, Martin Zeller, Daniel Hermanson, Hamish Stewart, Christian Hock, Alexander Makarov, Vlad Zabrouskov, Joshua J Coon
We describe deep analysis of the human proteome in less than one hour. We achieve this expedited proteome characterization by leveraging state-of-the-art sample preparation, chromatographic separations, data analysis tools, and by using the new Orbitrap Astral mass spectrometer equipped with a quadrupole mass filter, a high-field Orbitrap mass analyzer, and an asymmetric track lossless (Astral) mass analyzer. The system offers high MS/MS acquisition speed of 200 Hz and detects hundreds of peptide sequences per second within data independent- or data-dependent acquisition modes of operation...
April 3, 2024: Molecular & Cellular Proteomics: MCP