Damiola Francesca, Alberti Laurent, Mansuet-Lupo Audrey, Damotte Diane, Hofman Véronique, Tixier Lucie, Penault-Llorca Fréderique, Rouquette Isabelle, Vignaud Jean Michel, Cazes Aurelie, Forest Fabien, Begueret Hugues, Gibault Laure, Badoual Cécile, Cayre Anne, Taranchon-Clermont Estelle, Duc Adeline, Mc Leer Anne, Lantuejoul Sylvie
BACKGROUND: ALK, ROS1 and RET rearrangements occur respectively in 5%, 2% and 1% non-small cell lung cancers (NSCLC). ALK and ROS1 fusion proteins detection by immunohistochemistry (IHC) has been validated for rapid patient screening, but ROS1 fusions need to be confirmed by another technique and no RET IHC test is available for clinical use. RESEARCH DESIGN AND METHODS: We report herein the usefulness of the HTG EdgeSeq Assay, an RNA extraction-free test combining a quantitative nuclease protection assay with NGS, for the detection of ALK, ROS1 and RET fusions from 'real-life' small NSCLC samples...
October 31, 2023: Expert Review of Molecular Diagnostics