Zhao Jianbo, Wei Jun, Wang Xuanlin, Cao Hui
γ-polyglutamic acid (γ-PGA) is a biomarker that can be directly obtained by microbial fermentation. Poly(amino acid) superabsorbent polymers (SAPs) were prepared with purified γ-PGA as raw material and ethylene glycol diglycidyl ether (EGDGE) as a cross-linking agent. However, γ-PGA fermentation broth has a high viscosity, requires complex extraction and separation processes, and entails high energy consumption, resulting in the high cost of poly (amino acid) SAPs. Therefore, the coupling fermentation processes of glutamate polyglutamic acid, the process of using glutamate fermentation broth instead of pure glutamate powder for fermentation, and the process of treating the fermentation broth under conditions of centrifugation, UV irradiation, and high temperature, were studied...
October 31, 2023: BMC Biotechnology
M Golchin, S Mollayi, E Mohammadi, N Eskandarzade
BACKGROUND: Brucellosis remains one of the global health concerns that reemerges in recent years. Delayed or inaccurate diagnosis end to a long treatment duration and financial burden; therefore, finding a good antigen for detection of specific anti-Brucella antibodies is crucial. We intended to evaluate the serodiagnosis value of recombinant Brucella outer membrane protein 19 kDa (rOMP19) using indirect ELISA system compared with Rose Bengal test. RESULTS: The OMP19 sequence was successfully cloned into pET-28a and produced in E...
October 24, 2023: BMC Biotechnology
Jian He, Ping Liang, Tingting Wang, Shuang Han
A chemiluminescent immunoassay for human serum Cystatin C (Cys C) was established using a direct-antibody sandwich model. The immunoassay kit uses magnetic separation technology, using magnetic particles as the reaction solid phase, alkaline phosphatase as the marker enzyme, and a new chemiluminescent substrate APLS as the substrate. It has the characteristics of high sensitivity and short reaction time. This product uses high-affinity antibodies, resulting in a high specificity. The established method showed good accuracy, uniformity, and stability...
October 11, 2023: BMC Biotechnology
Yasser Tabana, Dinesh Babu, Richard Fahlman, Arno G Siraki, Khaled Barakat
Target identification is an essential part of the drug discovery and development process, and its efficacy plays a crucial role in the success of any given therapy. Although protein target identification research can be challenging, two main approaches can help researchers make significant discoveries: affinity-based pull-down and label-free methods. Affinity-based pull-down methods use small molecules conjugated with tags to selectively isolate target proteins, while label-free methods utilize small molecules in their natural state to identify targets...
October 10, 2023: BMC Biotechnology
Lu Shi, Tiantian Zhao, Lei Huang, Xiaomin Pan, Tianzhen Wu, Xin Feng, Taoli Chen, Jiamin Wu, Jianlou Niu
BACKGROUND: The major safety concern of the clinical application of wild type FGF19 (FGF19WT ) emerges given that its extended treatment causes hepatocellular carcinoma. Therefore, we previously generated a safer FGF19 variant - FGF19ΔKLB , which have same effects on glycemic control and bile acid production but much less mitogenic activity. However, it remains unclear as to whether FGF19ΔKLB ameliorates intrahepatic cholestasis. RESULTS: We found that, similar to that of FGF19WT , the chronic administration of FGF19ΔKLB protects mice from cholestatic liver injury in these two models...
October 3, 2023: BMC Biotechnology
Bahareh Nowruzi, Bilal Saad Jalil, James S Metcalf
Metal nanoparticles exhibit excellent antifungal abilities and are seen as a good substitute for controlling different kinds of fungi. Of all known taxa, cyanobacteria have received significant consideration as nanobiofactories, as a result of the cellular assimilation of heavy metals from the environment. The cellular bioactive enzymes, polysaccharides and pigments can be used as reducers and coatings during biosynthesis. The probability of the antifungal activity of selenium nanoparticles (SeNPs) to prevent plant fungi that can affect humans was evaluated and a toxic Iranian cyanobacterial strain of Desmonostoc alborizicum was used to study the biotechnology of SeNP synthesis for the first time...
September 27, 2023: BMC Biotechnology
Zahra Ahmadzadeh Chaleshtori, Ali Asghar Rastegari, Hashem Nayeri, Abbas Doosti
BACKGROUND: Helicobacter pylori cause a variety of gastric malignancies, gastric ulcers, and cause erosive diseases. The extreme nature of the bacterium and the implantation of this bacterium protects it against designing a potent drug against it. Therefore, employing a precise and effective design for a more safe and stable antigenic vaccine against this pathogen can effectively control its associated infections. This study, aimed at improving the design of multiple subunit vaccines against H...
September 27, 2023: BMC Biotechnology
Nikolaus I Stellner, Zora S Rerop, Norbert Mehlmer, Mahmoud Masri, Marion Ringel, Thomas B Brück
BACKGROUND: Cutaneotrichosporon oleaginosus is an oleaginous yeast that can produce up to 80% lipid per dry weight. Its high capacity for the biosynthesis of single cell oil makes it highly interesting for the production of engineered lipids or oleochemicals for industrial applications. However, the genetic toolbox for metabolic engineering of this non-conventional yeast has not yet been systematically expanded. Only three long endogenous promoter sequences have been used for heterologous gene expression, further three dominant and one auxotrophic marker have been established...
September 18, 2023: BMC Biotechnology
Ellahe Nouruzi, Seyed Mostafa Hosseini, Babak Asghari, Reza Mahjoub, Ehsan Nazarzadeh Zare, Mohammad-Ali Shahbazi, Fereshte Kalhori, Mohammad Reza Arabestani
Staphylococcus aureus is a unique challenge for the healthcare system because it can form biofilms, is resistant to the host's immune system, and is resistant to numerous antimicrobial therapies. The aim of this study was to investigate the effect of poly (lactic-co-glycolic acid) (PLGA) polymer nanoparticles loaded with vancomycin and conjugated with lysostaphin (PLGA-VAN-LYS) on inhibiting S. aureus biofilm formation. Nano drug carriers were produced using the double emulsion evaporation process. we examined the physicochemical characteristics of the nanoparticles, including particle size, polydispersity index (PDI), zeta potential, drug loading (DL), entrapment efficiency (EE), Lysostaphin conjugation efficiency (LCE), and shape...
September 18, 2023: BMC Biotechnology
Huiming Jiang, Jun Lu, Jiawei Li, Zizheng Liu, Fufei Chen, Rui Wu, Xingquan Xu, Yuan Liu, Yiqiu Jiang, Dongquan Shi
BACKGROUND: Cartilage defects are common sports injuries without significant treatment. Articular cartilage with inferior regenerative potential resulted in the poor formation of hyaline cartilage in defects. Acellular matrix scaffolds provide a microenvironment and biochemical properties similar to those of native tissues and are widely used for tissue regeneration. Therefore, we aimed to design a novel acellular cartilage matrix scaffold (ACS) for cartilage regeneration and hyaline-like cartilage formation...
September 14, 2023: BMC Biotechnology
Ramzi Shawahna, Hala Saba'aneh, Amal Daraghmeh, Yara Qassarwi, Valentina Franco, Xavier Declèves
BACKGROUND: Lamotrigine is an effective antiseizure medication that can be used in the management of focal and generalized epilepsies in pediatric patients. This study was conducted to quantify and compare the solubility of lamotrigine in age-specific biorelevant media that simulated the fasted and fed conditions of the gastric and intestinal environments in pediatrics and adults. Another aim was to predict how traditional, re-formulated, modified, and new oral formulations would behave in the gastric and intestinal environments across different age groups...
September 8, 2023: BMC Biotechnology
Riai Okamoto, Nobumasa Ito, Yutaro Ide, Bouchra Kitab, Yoshihiro Sakoda, Kyoko Tsukiyama-Kohara
BACKGROUND: Classical swine fever (CSF) is a fatal contagious disease affecting pigs caused by classical swine fever virus (CSFV). The disease can be transmitted by pigs and wild boars, and it is difficult to prevent and control. To obtain necessary information to establish the CSFV resistant animals in a future study, we designed lentiviral vector-delivered short hairpin RNAs (shRNAs) targeting the conserved domain III of the internal ribosomal entry site (IRES) of the CSFV genomic RNA...
September 8, 2023: BMC Biotechnology
David I Gonzalez, Ruby A Ynalvez
BACKGROUND: Biofuel research that aims to optimize growth conditions in microalgae is critically important. Chlamydomonas reinhardtii is a green microalga that offers advantages for biofuel production research. This study compares the effects of nitrogen-, sulfur-, and nitrogen and sulfur- deprivations on the C. reinhardtii starchless mutant cc5373-sta6. Specifically, it compares growth, lipid body accumulation, and expression levels of acetyl-CoA carboxylase (ACC) and phosphoenolpyruvate carboxylase (PEPC)...
September 8, 2023: BMC Biotechnology
Paul-Joachim Niehoff, Waldemar Müller, Johannes Pastoors, Katharina Miebach, Philipp Ernst, Johannes Hemmerich, Stephan Noack, Nick Wierckx, Jochen Büchs
BACKGROUND: Currently, Aspergillus terreus is used for the industrial production of itaconic acid. Although, alternative feedstock use in fermentations is crucial for cost-efficient and sustainable itaconic acid production, their utilisation with A. terreus most often requires expensive pretreatment. Ustilaginacea are robust alternatives for itaconic acid production, evading the challenges, including the pretreatment of crude feedstocks regarding reduction of manganese concentration, that A...
September 3, 2023: BMC Biotechnology
Siyuan Gao, Ding Ma, Yongtao Wang, Alei Zhang, Xin Wang, Kequan Chen
Dopamine is high-value compound of pharmaceutical interest, but its industrial scale production mostly focuses on chemical synthesis, possessing environment pollution. Bio-manufacturing has caused much attention for its environmental characteristic. Resting cells were employed to as biocatalysts with extraordinary advantages like offering stable surroundings, the inherent presence of expensive cofactors. In this study, whole-cell bioconversion was employed to convert dopa to dopamine. To increase the titer and yield of dopamine production through whole-cell catalysis, three kinds of aromatic amino acid transport protein, AroP, PheP and TyrP, were selected to be co-expressed...
August 29, 2023: BMC Biotechnology
Sixun Chen, Ahmad Amirul Bin Abdul Rahim, Pamela Mok, Dan Liu
BACKGROUND: The in-vitro scratch assay is a useful method in wound healing research to assess cell migration. In this assay, a scratch is created in a confluent cell layer by mechanically removing cells through manual scraping with a sharp-edged tool. This step is traditionally done with pipette tips and is unsuitable for high-throughput assays, as the created scratches are highly variable in width and position. Commercially available solutions are often expensive, and require specific cultureware which might not be suitable for all studies...
August 28, 2023: BMC Biotechnology
Iván Hernández-Neuta, Anastasia Magoulopoulou, Flor Pineiro, Jan Gorm Lisby, Mats Gulberg, Mats Nilsson
BACKGROUND: Global efforts to characterize diseases of poverty are hampered by lack of affordable and comprehensive detection platforms, resulting in suboptimal allocation of health care resources and inefficient disease control. Next generation sequencing (NGS) can provide accurate data and high throughput. However, shotgun and metagenome-based NGS approaches are limited by low concentrations of microbial DNA in clinical samples, requirements for tailored sample and library preparations plus extensive bioinformatics analysis...
August 23, 2023: BMC Biotechnology
Chang Liu, Cuicui Chen, Fenglan Peng, Huankun Liang, Shuhai Zhong, Tiancai Liu, Laiqing Li, Alexander Boronin, Wenqi Dong
BACKGROUND: Carbohydrate antigen 724 (CA724) is a sensitive and specific indicator for multiple malignant tumors. The aim of this study was to establish a Eu-time resolved fluorescence immunochromatography (Eu-TRFICO) method for quantitative detection of CA724 in serum. METHODS: Eu-TRFICO strips were optimized and assembled. The sensitivity, specificity and precision were evaluated using CA724 standard dilutions and matrix serum. Meanwhile, the reference interval, comparison, and sensitivity/specificity were performed using clinical negative/positive gastric cancer serum samples...
August 18, 2023: BMC Biotechnology
Weiwei Liu, Xiaoguo Wang, Ruirong Liu, Yaya Liao, Zhiwei Peng, Haoyun Jiang, Qiqi Jing, Yuyun Xing
BACKGROUND: Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory especially when large-size vectors are to be delivered. In this study, we aimed to optimize the transfection conditions for delivery of a large-size vector in PFFs using Lonza 4D-Nucleofector™ vessels and strips. METHODS: We firstly delivered a 13 kb Cas9-EGFP and a 3.5 kb pMAX-GFP vector into PFFs via 7 programs recommended by the Lonza basic protocol...
August 16, 2023: BMC Biotechnology
Asmaa E Farouk, Sohair R Fahmy, Amel M Soliman, Sherif Abdelaziz Ibrahim, Shimaa A Sadek
BACKGROUND: Coelomic fluid, a pharmacologically active compound in earthworms, exhibits a range of biological activities, including antioxidant, anti-inflammatory, and anticancer. However, the biological activities exerted by the coelomic fluid can be restrained by its low bioavailability and stability. Liposomes are progressively utilized as an entrapment system for natural bioactive compounds with poor bioavailability and stability, which could be appropriate for coelomic fluid...
August 3, 2023: BMC Biotechnology
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