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BMC Biotechnology

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https://read.qxmd.com/read/30777060/ex-vivo-evolution-of-human-antibodies-by-crispr-x-from-a-naive-b-cell-repertoire-to-affinity-matured-antibodies
#1
Marie-Claire Devilder, Melinda Moyon, Laetitia Gautreau-Rolland, Benjamin Navet, Jeanne Perroteau, Florent Delbos, Marie-Claude Gesnel, Richard Breathnach, Xavier Saulquin
No abstract text is available yet for this article.
February 18, 2019: BMC Biotechnology
https://read.qxmd.com/read/30770741/a-new-prokaryotic-expression-vector-for-the-expression-of-antimicrobial-peptide-abaecin-using-sumo-fusion-tag
#2
Da Sol Kim, Seon Woong Kim, Jae Min Song, Soon Young Kim, Kwang-Chul Kwon
BACKGROUND: Despite the growing demand for antimicrobial peptides (AMPs) for clinical use as an alternative approach against antibiotic-resistant bacteria, the manufacture of AMPs relies on expensive, small-scale chemical methods. The small ubiquitin-related modifier (SUMO) tag is industrially practical for increasing the yield of recombinant proteins by increasing solubility and preventing degradation in expression systems. RESULTS: A new vector system, pKSEC1, was designed to produce AMPs, which can work in prokaryotic systems such as Escherichia coli and plant chloroplasts...
February 15, 2019: BMC Biotechnology
https://read.qxmd.com/read/30760266/correction-to-characterizing-active-transportation-mechanisms-for-free-fatty-acids-and-antibiotics-in-synechocystis-sp-pcc-6803
#3
Matthew P A Bellefleur, Soo-Young Wanda, Roy Curtiss Iii
Following publication of the original article [1], the author reported that the gene/protein names of slr2131 and sll0180 were swapped in the Discussion section. The details of the correction are mentioned below.
February 13, 2019: BMC Biotechnology
https://read.qxmd.com/read/30744615/production-of-high-titer-of-citric-acid-from-inulin
#4
Magdalena Rakicka, Jakub Wolniak, Zbigniew Lazar, Waldemar Rymowicz
BACKGROUND: Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study citric acid was synthesized by genetically engineered strains of Yarrowia lipolytica from widely available, renewable polysaccharide - inulin. Hydrolysis of inulin by the Y. lipolytica strains was established by expressing the inulinase gene (INU1 gene; GenBank: X57202...
February 11, 2019: BMC Biotechnology
https://read.qxmd.com/read/30691440/resveratrol-alleviates-lipopolysaccharide-induced-inflammation-in-pc-12-cells-and-in-rat-model
#5
Guiqi Zhang, Yi Liu, Lichen Xu, Chunhe Sha, Haibin Zhang, Weibing Xu
BACKGROUND: Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in inflammation induced by LPS. RESULTS: RSV improved inflammatory injury and functional recovery in rat model of SCI. RSV inhibited LPS-induced inflammatory injury in PC-12 cells via increasing viability, decreasing apoptosis, and suppressing IL-1β, IL-6, and TNF-α expression...
January 28, 2019: BMC Biotechnology
https://read.qxmd.com/read/30691438/crispr-cas9-mediated-targeted-disruption-of-fad2-2-microsomal-omega-6-desaturase-in-soybean-glycine-max-l
#6
Noor Al Amin, Naveed Ahmad, Nan Wu, Xiumin Pu, Tong Ma, Yeyao Du, Xiaoxue Bo, Nan Wang, Rahat Sharif, Piwu Wang
BACKGROUND: Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system has appeared a promising tool in site-directed mutagenesis due to its innovative utilization in different branches of biology. CRISPR-Cas9 nuclease system have supersedes all previously existed strategies and their associated pitfalls encountered with site-specific mutagenesis...
January 28, 2019: BMC Biotechnology
https://read.qxmd.com/read/30678667/extracellular-overproduction-of-e7-oncoprotein-of-iranian-human-papillomavirus-type-16-by-genetically-engineered-lactococcus-lactis
#7
Amir Hossein Mohseni, Sedigheh Taghinezhad-S, Hossein Keyvani, Vadood Razavilar
BACKGROUND: We aimed at constructing Lactococcus lactis strains expressing HPV-16 recombinant E7 (rE7) oncoprotein and examining its overproduction ability followed by optimizing batch and fed-batch fermentations. Thereafter, in order to assess the immunogenicity of recombinant L. lactis cells, C57BL/6 mice were immunized by oral gavage. RESULTS: The results suggested that recombinant strains harboring optiE7 and E7 genes produced a maximum of 4.84 and 1.91 μg/mL of rE7 in static flask experiments, while the corresponding strains gave a maximum yield of 35...
January 24, 2019: BMC Biotechnology
https://read.qxmd.com/read/30646876/homologous-recombination-mediated-targeted-integration-in-monkey-embryos-using-tale-nucleases
#8
Chu Chu, Zhaohui Yang, Jiayin Yang, Li Yan, Chenyang Si, Yu Kang, Zhenzhen Chen, Yongchang Chen, Weizhi Ji, Yuyu Niu
BACKGROUND: Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system. It has been demonstrated that these programmable nucleases can introduce genetic changes in embryos from many species including NHPs...
January 15, 2019: BMC Biotechnology
https://read.qxmd.com/read/30646874/microrna-218-promotes-early-chondrogenesis-of-mesenchymal-stem-cells-and-inhibits-later-chondrocyte-maturation
#9
Song Chen, Zhenyu Xu, Jiahua Shao, Peiliang Fu, Haishan Wu
BACKGROUND: MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate chondrogenic differentiation of synovium-derived MSCs (SDSCs) and the maturation of RCJ3.1C5.1 chondrocytes. SDSCs were sourced from the knee joint synovium of New Zealand white rabbits, and their multilineage differentiation potentials were examined. The level of miR-218 was measured during SDSC chondrogenic differentiation, together with determination of SDSCs chondrogenic markers and RCJ3...
January 15, 2019: BMC Biotechnology
https://read.qxmd.com/read/30630457/characterizing-active-transportation-mechanisms-for-free-fatty-acids-and-antibiotics-in-synechocystis-sp-pcc-6803
#10
Matthew P A Bellefleur, Soo-Young Wanda, Roy Curtiss
BACKGROUND: Synechocystis sp. PCC 6803 is a photosynthetic bacterium that has been genetically modified to produce industrially relevant chemicals, yet efflux mechanisms have not been well elucidated. These photosynthetic organisms live in environments that are often nutrient limited; therefore, the genome of these organisms encodes far fewer proteins used for efflux of chemicals when compared to members of the Enterobacteriaceae family. Understanding efflux mechanisms can lead to a greater efficiency of chemical production within the cyanobacterial cell...
January 10, 2019: BMC Biotechnology
https://read.qxmd.com/read/30630453/evaluation-of-strategies-for-improving-the-transgene-expression-in-an-oleaginous-microalga-scenedesmus-acutus
#11
Anongpat Suttangkakul, Anchalee Sirikhachornkit, Piyada Juntawong, Wilasinee Puangtame, Thitikorn Chomtong, Suchada Srifa, Sukhita Sathitnaitham, Wasawat Dumrongthawatchai, Kanidtha Jariyachawalid, Supachai Vuttipongchaikij
BACKGROUND: Genetic transformation of microalgae has been hampered by inefficient transgene expression, limiting the progress of microalgal biotechnology. Many vector tools and strategies have been developed in recent years to improve transgene expression in the model microalga Chlamydomonas, but these were hardly applied to other microalgae. In this work, naturally-isolated oleaginous microalgae were accessed for genetic transformation, and various expression systems were evaluated in a selected microalga to circumvent inefficient transgene expression...
January 10, 2019: BMC Biotechnology
https://read.qxmd.com/read/30626373/fast-gene-disruption-in-trichoderma-reesei-using-in-vitro-assembled-cas9-grna-complex
#12
Zhenzhen Hao, Xiaoyun Su
BACKGROUND: CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei, a filamentous fungus workhorse for cellulase production. However, expression of Cas9 heterologously in the host cell could be time-consuming and sometimes even troublesome. RESULTS: We tested two gene disruption methods in T. reesei using CRISPR/Cas9 in this study. The intracellularly expressed Cas9 led to unexpected off-target gene disruption in T...
January 9, 2019: BMC Biotechnology
https://read.qxmd.com/read/30626366/an-auto-inducible-phosphate-controlled-expression-system-of-bacillus-licheniformis
#13
Nguyen Thanh Trung, Nguyen Minh Hung, Nguyen Huy Thuan, Nguyen Xuan Canh, Thomas Schweder, Britta Jürgen
BACKGROUND: A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in the absence of an artificial inducer would facilitate the efficient production of heterologous proteins at low cost. A novel phosphate-regulated expression system was constructed using the promoter of the phytase encoding gene phyL from Bacillus licheniformis for the overexpression of proteins in this industrially relevant host. RESULTS: It is shown that the phyL promoter enables a strong overexpression of the heterologous genes amyE and xynA in B...
January 9, 2019: BMC Biotechnology
https://read.qxmd.com/read/30606176/establishment-of-tp53-knockout-canine-cells-using-optimized-cripsr-cas9-vector-system-for-canine-cancer-research
#14
Kiyoung Eun, Min Gi Park, Yeon Woo Jeong, Yeon Ik Jeong, Sang-Hwan Hyun, Woo Suk Hwang, Sung-Hak Kim, Hyunggee Kim
BACKGROUND: Genetic engineering technology such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides a powerful tool for developing disease models and determining gene functions. Recent interests in canine cancer models have highlighted the necessity of developing genetic engineering tools for dogs. In this study, we attempted to generate optimized CRISPR/Cas9 system to target canine tumor protein 53 (TP53), one of the most crucial tumor suppressor genes, to establish TP53 knockout canine cells for canine cancer research...
January 3, 2019: BMC Biotechnology
https://read.qxmd.com/read/30594179/alterations-in-the-proteomic-composition-of-serratia-marcescens-in-response-to-manganese-ii
#15
Pollyana Santos Queiroz, France Anne Dias Ruas, Natália Rocha Barboza, William de Castro Borges, Renata Guerra-Sá
BACKGROUND: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). RESULTS: The LG1 strain, which grew equally well in the two conditions, was found to express a set of proteins related to cellular processes vital for survival, as well as proteins involved in adaptation and tolerance to Mn (II)...
December 29, 2018: BMC Biotechnology
https://read.qxmd.com/read/30594166/a-chicken-bioreactor-for-efficient-production-of-functional-cytokines
#16
Lissa R Herron, Clare Pridans, Matthew L Turnbull, Nikki Smith, Simon Lillico, Adrian Sherman, Hazel J Gilhooley, Martin Wear, Dominic Kurian, Grigorios Papadakos, Paul Digard, David A Hume, Andrew C Gill, Helen M Sang
BACKGROUND: The global market for protein drugs has the highest compound annual growth rate of any pharmaceutical class but their availability, especially outside of the US market, is compromised by the high cost of manufacture and validation compared to traditional chemical drugs. Improvements in transgenic technologies allow valuable proteins to be produced by genetically-modified animals; several therapeutic proteins from such animal bioreactors are already on the market after successful clinical trials and regulatory approval...
December 29, 2018: BMC Biotechnology
https://read.qxmd.com/read/30587177/enhancing-the-efficiency-of-the-pichia-pastoris-aox1-promoter-via-the-synthetic-positive-feedback-circuit-of-transcription-factor-mxr1
#17
Ching-Hsiang Chang, Hao-An Hsiung, Kai-Lin Hong, Ching-Tsan Huang
BACKGROUND: The methanol-regulated AOX1 promoter (PAOX1 ) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol dependence of PAOX1 restricts its application, it is necessary to develop a flexible induction system to avoid the problems of methanol without losing the advantages of PAOX1 . The availability of synthetic biology tools enables researchers to reprogram the cellular behaviour of P...
December 27, 2018: BMC Biotechnology
https://read.qxmd.com/read/30547780/recombinant-expression-and-bioactivity-comparison-of-four-typical-fungal-immunomodulatory-proteins-from-three-main-ganoderma-species
#18
Zheng-Wei Qu, Si-Ya Zhou, Shi-Xin Guan, Rui Gao, Zuo-Wen Duan, Xin Zhang, Wei-Yan Sun, Wen-Li Fan, Shui-Sen Chen, Li-Jing Chen, Jing-Wei Lin, Yan-Ye Ruan
BACKGROUND: More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap2 from G. applanatum, along with LZ-8 and FIP-gsi, another two representative Ganoderma FIP genes from G. lucidum and G. sinense were functionally expressed in Pichia. Subsequently, bioactivities of four recombinant Ganoderma FIPs were demonstrated and compared...
December 14, 2018: BMC Biotechnology
https://read.qxmd.com/read/30541521/simultaneous-enzymatic-saccharification-and-comminution-for-the-valorization-of-lignocellulosic-biomass-toward-natural-products
#19
Ronald R Navarro, Yuichiro Otsuka, Masanobu Nojiri, Shigehiro Ishizuka, Masaya Nakamura, Kazuhiro Shikinaka, Kenji Matsuo, Kei Sasaki, Ken Sasaki, Kazuhide Kimbara, Yutaka Nakashimada, Junichi Kato
BACKGROUND: Large-scale processing of lignocellulosics for glucose production generally relies on high temperature and acidic or alkaline conditions. However, extreme conditions produce chemical contaminants that complicate downstream processing. A method that mainly rely on mechanical and enzymatic reaction completely averts such problem and generates unmodified lignin. Products from this process could find novel applications in the chemicals, feed and food industry. But a large-scale system suitable for this purpose is yet to be developed...
December 12, 2018: BMC Biotechnology
https://read.qxmd.com/read/30541520/association-of-high-pressure-and-alkaline-condition-for-solubilization-of-inclusion-bodies-and-refolding-of-the-ns1-protein-from-zika-virus
#20
Cleide Mara Rosa da Silva, Rosa Maria Chura-Chambi, Lennon Ramos Pereira, Yraima Cordeiro, Luís Carlos de Souza Ferreira, Ligia Morganti
BACKGROUND: Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor recovery of bioactive proteins. High hydrostatic pressure (HHP) and alkaline pH are two conditions that, in the presence of low level of chaotropes, have been described as non-denaturing solubilization agents...
December 12, 2018: BMC Biotechnology
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