Xinwei Zhao, Dongping Yu, Weijia Zhou, Long Yu, Han Zhou, Yanfang Liu, Zhimou Guo, Aijing Shen, Ziwei Han, Chaoran Wang, Jixia Wang, Xinmiao Liang
Ziziphi spinosae semen has been widely used to treat insomnia and anxiety. To profile its chemical components, an online comprehensive two-dimensional LC-MS was developed. In this two-dimensional LC system, a novel phthalic anhydride bonded stationary phase column was combined with a C18 column. As a result, this new stationary phase exhibited remarkable differences in separation selectivity from C18, achieving a good orthogonality of 83.3%. Moreover, this new stationary phase with weaker hydrophobicity than C18 realized solvent compatibility in the online configuration...
March 9, 2023: Journal of Separation Science