journal
https://read.qxmd.com/read/38867483/determination-of-physicochemical-parameters-of-bio-molecules-and-bio-particles-by-capillary-electromigration-methods
#1
REVIEW
Sille Štěpánová, Václav Kašička
The review provides an overview of recent developments and applications of capillary electromigration (CE) methods for the determination of important physicochemical parameters of various (bio)molecules and (bio)particles. These parameters include actual and limiting (absolute) ionic mobilities, effective electrophoretic mobilities, effective charges, isoelectric points, electrokinetic potentials, hydrodynamic radii, diffusion coefficients, relative molecular masses, acidity (ionization) constants, binding constants and stoichiometry of (bio)molecular complexes, changes of Gibbs free energy, enthalpy and entropy and rate constants of chemical reactions and interactions, retention factors and partition and distribution coefficients...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38863110/a-sensitive-bioanalytical-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-method-for-the-simultaneous-quantitation-of-lactone-and-carboxylate-forms-of-topotecan-in-plasma-and-vitreous
#2
JOURNAL ARTICLE
Barbora Mudrova, Katerina Hrabakova, Petr Kozlik, Radka Hobzova, Jakub Sirc, Zuzana Bosakova
Topotecan (TPT) is used in the treatment of retinoblastoma, the most common malignant intraocular tumor in children. TPT undergoes pH-dependent hydrolysis of the lactone ring to the ring-opened carboxylate form, with the lactone form showing antitumor activity. A selective, and highly sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry method was developed for the determination of both forms of TPT in one mobile phase composition in plasma and vitreous humor matrices. The method showed an excellent linear range of 0...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38863086/determination-of-the-binding-constants-and-ionic-mobilities-of-diquat-complexes-with-randomly-sulfated-cyclodextrins-by-affinity-capillary-electrophoresis
#3
JOURNAL ARTICLE
Jan Bílek, Dušan Koval, Veronika Šolínová, Harish L Talele, Lukáš Severa, Paul E Reyes Gutiérrez, Filip Teplý, Václav Kašička
The enantiomers of diquats (DQs), a new class of functional organic molecules, were recently separated by capillary electrophoresis (CE) with high resolution up to 11.4 within 5-7 min using randomly sulfated α-, β-, and γ-cyclodextrins (CDs) as chiral selectors. These results indicated strong interactions between dicationic diquats and multiply negatively charged sulfated CDs (S-CDs). However, the binding strength of these interactions was not quantified. For that reason, in this study, affinity CE was applied for the determination of the binding constants and ionic mobilities of the complexes of DQ P- and M-enantiomers with CD chiral selectors in an aqueous medium...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38863084/recent-advances-in-microscale-separation-techniques-for-glycome-analysis
#4
REVIEW
Chenchen Liu, Koji Otsuka, Takayuki Kawai
The glycomic analysis holds significant appeal due to the diverse roles that glycans and glycoconjugates play, acting as modulators and mediators in cellular interactions, cell/organism structure, drugs, energy sources, glyconanomaterials, and more. The glycomic analysis relies on liquid-phase separation technologies for molecular purification, separation, and identification. As a miniaturized form of liquid-phase separation technology, microscale separation technologies offer various advantages such as environmental friendliness, high resolution, sensitivity, fast speed, and integration capabilities...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38847294/design-of-double-core-shell-structural-materials-for-the-extraction-of-non-steroidal-anti-inflammatory-drugs
#5
JOURNAL ARTICLE
Hongmei Liu, Shihao Dang, Baogui Ye, Hua Fan, Caihong Tao
In this work, core-shell material with a special structure was designed and applied in solid-phase extraction (SPE) for non-steroidal anti-inflammatory drugs (NSAIDs) combined with high-performance liquid chromatography. Based on the advantages of core-shell ZIF-8@ZIF-67 (Zeolite imidazole ester framework materials [ZIFs]), effective derivatization treatment was carried out to partially vulcanize the original ZIFs, resulting in a special and new double-core-shell structural material CoS/ZIF-67/ZnS/ZIF-8 (ZIFs@ZnS@CoS) with porous surface and center hollow...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38822226/improvement-of-oligonucleotide-separation-using-a-repetto-high-performance-liquid-chromatography-recycling-approach
#6
JOURNAL ARTICLE
Barbora Fiserova, Milan Minarik, Michal Nahunek, Roman Kysilka, Marek Minarik
A new approach for the improvement of separation of oligonucleotides by recycling ion-pairing chromatography is described. In the so-called repetto process, segments of separated compounds are sequentially returned to the inlet for multiple passages through the column without a need to pass a pump and with the possibility of detecting the level of separation between individual passages. Unlike in the recently described twin-column recycle approach in which eluents are repeatedly transferred between two separation columns, with the repetto method a single column is sufficient, and the detector is not exposed to high back pressure...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38822225/separation-of-bufadienolides-from-helleborus-thibetanus-franch-by-a-combination-approach-involving-macroporous-resin-column-chromatography-and-gradient-countercurrent-chromatography
#7
JOURNAL ARTICLE
Jinxing Guo, Feng Chen, Wenzhen Zhang, Huiyuan Bai, Luqi Li, Yatuan Ma, Zhi Yang
In this study, a combination approach involving macroporous resin (MR) column chromatography and gradient countercurrent chromatography (CCC) was employed to enrich and purify bufadienolides from the roots and rhizomes of Helleborus thibetanus Franch. Initially, a D101 MR-packed column chromatography was utilized for fractionation and enrichment of the bufadienolides, which were effectively eluted from the column using a 60% ethanol solution. CCC was subsequently introduced to separate the enriched product using the ethyl acetate/n-butanol/water (EBuWat, 4:1:5, v/v) and EBuWat (5:0:5, v/v) solvent systems in a gradient elution mode...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819868/deep-learning-framework-for-peak-detection-at-the-intact-level-of-therapeutic-proteins
#8
JOURNAL ARTICLE
Saxena Nikita, Sanghati Bhattacharya, Kriti Manocha, Anurag S Rathore
While automated peak detection functionalities are available in commercially accessible software, achieving optimal true positive rates frequently necessitates visual inspection and manual adjustments. In the initial phase of this study, hetero-variants (glycoforms) of a monoclonal antibody were distinguished using liquid chromatography-mass spectrometry, revealing discernible peaks at the intact level. To comprehensively identify each peak (hetero-variant) in the intact-level analysis, a deep learning approach utilizing convolutional neural networks (CNNs) was employed in the subsequent phase of the study...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819804/carboxyl-functionalized-covalent-organic-frameworks-for-the-extraction-of-malachite-green-and-crystal-violet-in-environmental-water-samples-prior-to-quantification-by-high-performance-liquid-chromatography
#9
JOURNAL ARTICLE
Bingmei Lai, Haoyun Hu, Ningli Tang, Yipeng Huang, Guihua Ruan
In this study, monodisperse, uniform, and spherical covalent organic frameworks (COFs) were synthesized using 1,3,5-tris (4-aminophenyl) benzene and 1,3,5-tricarboxaldehyde benzene at room temperature. Post-modification of 6-aminocaproic acid on the COFs yielded carboxyl-modified COFs (COFs-COOH). The modification enhanced the hydrophilicity and adsorption efficiencies of COFs-COOH for malachite green (MG) and crystal violet (CV). A COFs-COOH-based dispersive solid-phase extraction coupled with high-performance liquid chromatography was developed for the analysis of MG and CV...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819794/metabolomics-analysis-reveals-characteristic-metabolites-in-different-levels-of-oxaliplatin-induced-neurotoxicity
#10
JOURNAL ARTICLE
Yujiao Hua, Rong Wang, Yankui Liu, Quan Liu, Xiaowei Qi, Yongjuan Ding, Juan Lv
Oxaliplatin (L-OHP), a third-generation platinum-based anti-tumor drug, finds widespread application in the first-line treatment of metastatic colorectal cancer. Despite its efficacy, the drug's usage is curtailed by a litany of side effects, with L-OHP-induced peripheral neuropathy (OIPN) being the most debilitating. This condition can be classified into varying degrees of severity. Employing serum metabolomics, a high-sensitivity, high-throughput technique, holds promise as a method to identify biomarkers for clinical assessment and monitoring of OIPN patients across different severity levels...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819793/separation-identification-and-fingerprinting-of-antioxidant-components-in-persimmon-diospyros-kaki-leaves-by-offline-two-dimensional-liquid-chromatography-with-electrochemical-detection-and-tandem-mass-spectrometry
#11
JOURNAL ARTICLE
Xijin Yang, Qiju Shao, Qiong Luo, Yan Wang, Zeli Chun, Zhirong Li, Lingyi Jiao, Yaping Zhou, Rongxiang Chen
In this work, the antioxidant components in persimmon (Diospyros kaki) leaves were separated by offline two-dimensional liquid chromatography-electrochemical detection (LC×LC-ECD) and identified by LC-tandem mass spectrometry (LC-MS/MS). A total of 33 antioxidants, mainly proanthocyanidins, and glycosides of kaempferol and quercetin, were identified. The antioxidant assays demonstrated that the fractions collected from the first-dimension LC (1 D-LC) possessed considerable radical scavenging capabilities, with correlation coefficients of peak area versus radical scavenging capability of 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) being 0...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819790/alien-species-water-hyacinth-realizes-waste-into-treasure-the-preparation-of-biomass-sorbent-to-determine-benzoylurea-insecticides-in-tea-products
#12
JOURNAL ARTICLE
Hongmei Liu, Baogui Ye, Zhongwei Zhao, Mengyun Liu, Fangbin Fan, Caihong Tao
A fast and effective analytical method with biomass solid-phase microextraction sorbent combined with a high-performance liquid chromatography-ultraviolet detector was proposed for the determination of benzoylurea (BU) insecticides in tea products. The novel sorbent was prepared by activating and then carbonizing water hyacinth with a fast growth rate and low application value as raw material and showed a high specific surface area and multiple interactions with analytes, such as electrostatic action, hydrogen bonding, and π-π conjugation...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819785/applications-of-magnetic-solid-phase-extraction-in-the-sample-preparation-of-natural-product-analysis-2020-2023
#13
REVIEW
Ling-Xiao Chen, Feng-Qing Yang
Sample preparation, including extraction, separation, and purification, is a vital process for natural product analysis. As an attractive sample pretreatment method, magnetic solid-phase extraction (MSPE) has gained plenty of attention, mainly due to its simpler operation, less consumption of organic solvents, and shorter processing time than traditional SPE. This updated review is devoted to summarizing the applications of MSPE based on different magnetic nanomaterials in the analysis of various natural products in complex matrixes, such as biological samples, plants, and Chinese herbal preparations in the past four years (2020-2023)...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819784/screening-anti-anaphylactoid-components-in-polygonum-cuspidatum-via-cell-membrane-chromatography-with-lc-ms-targeting-mas-related-g-protein-coupled-receptor-x2
#14
JOURNAL ARTICLE
Yuanyuan Lin, Huaqiu Lu, Qianqian Jia, Shengli Han
Mas-related G protein-coupled receptor X2 (MrgprX2) is acknowledged as a mast cell-specific receptor, playing a crucial role in orchestrating anaphylactoid responses through mast cell degranulation. It holds promise as a target for regulating allergic and inflammatory diseases mediated by mast cells. Polygonum cuspidatum (PC) has shown notable anti-anaphylactoid effects, while its pharmacologically active components remain unclear. In this study, we successfully utilized MrgprX2 high-expressing cell membrane chromatography (CMC), in conjunction with liquid chromatography-mass spectrometry (LC-MS), to identify active anti-anaphylactoid components in PC...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819782/a-ternary-correlation-multi-symptom-network-strategy-based-on-in-vivo-chemical-profile-identification-and-metabolomics-to-explore-the-molecular-basis-of-ephedra-herb-against-viral-pneumonia
#15
JOURNAL ARTICLE
Xiaoyan Wang, Jie Mei, Fan Zhang, Miaomiao Wei, Yujun Xie, Alamusi Bayoude, Xiufeng Liu, Boli Zhang, Boyang Yu
Ephedra herb (EH), an important medicine prescribed in herbal formulas by Traditional Chinese Medicine practitioners, has been widely used in the treatment of viral pneumonia in China. However, the molecular basis of EH in viral pneumonia remains unclear. In this study, a ternary correlation multi-symptom network strategy was established based on in vivo chemical profile identification and metabolomics to explore the molecular basis of EH against viral pneumonia. Results showed that 143 compounds of EH and 70 prototype components were identified in vivo...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819781/preparation-of-carboxyl-functionalized-silica-core-shell-microspheres-and-their-applications-in-weak-cation-exchange-chromatography-heavy-metal-removal-and-lysozyme-enrichment
#16
JOURNAL ARTICLE
Shadi Ali Hassen Ahmed, Bassam Saif, Qian LingHui
Chromatography is a technique of separation based on adsorption and/or interaction of target molecules with stationary phases. Herein, we report the design and fabrication of BTDA@SiO2 core-shell microspheres as a new class of stationary phase and demonstrate its impressive performance for chromatographic separations. The silica microspheres of BTDA@SiO2 were synthesized by in situ method with 1,3,5-benzenetricarboxaldehyde and 3,5-diaminobenzoic to separate peptides and proteins on high-performance liquid chromatography...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819780/-sweet-space-strategy-for-solvent-system-selection-in-countercurrent-chromatography-a-case-study-on-separation-of-polyunsaturated-fatty-acids-from-borage-oil
#17
JOURNAL ARTICLE
Tian Han, Zhuo Fu, Muyun Yang, Xueli Cao
This study presents a comprehensive strategy for the selection and optimization of solvent systems in countercurrent chromatography (CCC) for the effective separation of compounds. With a focus on traditional organic solvent systems, the research introduces a "sweet space" strategy that merges intuitive understanding with mathematical accuracy, addressing the significant challenges in solvent system selection, a critical bottleneck in the widespread application of CCC. By employing a combination of volume ratios and graphical representations, including both regular and trirectangular tetrahedron models, the proposed approach facilitates a more inclusive and user-friendly strategy for solvent system selection...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819776/identity-verification-of-monoclonal-antibodies-by-triple-injection-capillary-zone-electrophoresis
#18
JOURNAL ARTICLE
Johan A Carlsson, Maria Löfgren, Ahmad Amini
This paper presents an approach based on triple injection capillary zone electrophoresis for identification of monoclonal antibodies. The analyte to be identified is injected between two zones of a known reference. The distances between the reference zones (plug I and III) and the target zone (plug II) are adjusted by partial electrophoresis of the first and second injection plugs. The full migration time of the target analyte is calculated from the observed migration time by considering the migration times of the reference in the first and third injection plugs...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819762/analytical-strategies-to-identify-multicomponent-quality-markers-for-commercial-hua-ju-hong-using-multidimensional-chemical-analysis
#19
JOURNAL ARTICLE
Chuchu Zhong, Yuting Wu, Changhong Cao, Danlin Lin, Jinju Zhang, Fan Wu, Jing Deng, Zhiguo Ma, Ying Zhang, Hui Cao, Menghua Wu
Hua-ju-hong (HJH) is a Chinese medicinal material obtained from Citrus grandis 'Tomentosa' (CGT) and Citrus grandis (L.) Osbeck (CG) with various commercial specifications. It is known for relieving cough and dispelling phlegm. To reveal the quality marker for distinguishing the various HJH, 215 batches of commercial HJH were studied systematically using multidimensional chemical analysis. Ten major components were identified by high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry and quantified via high-performance liquid chromatography coupled with diode array detection...
June 2024: Journal of Separation Science
https://read.qxmd.com/read/38819739/harnessing-an-amide-based-covalent-organic-framework-in-solid-phase-extraction-for-chlorophenol-analysis-in-industrial-wastewaters
#20
JOURNAL ARTICLE
Masoumeh Firouzy, Alireza Ghiasvand, Payman Hashemi
An amide-based covalent organic framework (COF) was successfully synthesized using the reaction between 1,3,5-trimesoyl chloride and ethylenediamine. The structure and morphology of the COF were characterized using Fourier-transform infrared spectra, nuclear magnetic resonance spectroscopy, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Brunauer-Emmett-Teller surface area analysis. The COF was employed as a solid-phase extraction adsorbent for the sampling and preconcentration of chlorophenols from industrial wastewater samples prior to high-performance liquid chromatography with ultraviolet detection...
June 2024: Journal of Separation Science
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