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Zhongguo Shi Yan Xue Ye Xue za Zhi

Ling Qi, Fei Li
Intestinal microbiome closely relates with human health and disease, which plays a critical role in the immune response, homeostasis, drug metabolism and tumorigenesis. Imbalances in the composition and function of these intestinal microbes associate with diseases. Fecal microbiota transplantation (FMT) is an established successful treatment modality for recurrent Clostridium difficile infection (CDI). The safety profile and potential therapeutic advantages of FMT for diseases associated with dysbiosis and immune dysfunction have led to many publications, mainly case series...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Zhen-Hua Wang, Ming Xu, Xiao-Lin Gu, Zhi Xu, Fei-Yan Yu
Lymphomas are traditionally divided into Hodgkin's lymphoma and non-Hodgkin's lymphoma(NHL), the NHL is a common hematological cancer, which represents a wide spectrum of illnesses from the most indolent to the most aggressive malignancies, and the detection of related molecular targets will be needed for diagosing each subtype of NHL. Advances in understanding the pathogenesis of NHL have improved the precision of diagnosis and the prognosis evaluation of patients with this disorder, such as chromosomal translocation leading to the up-regulation of oncogene expression...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Hui-Min Tang, Yu-Feng Li
As a potential target for cancer treatment, the C-Myc high expresses abnormally in a variety of solid tumors and hematological malignancies in humans. In hematologic malignancies, the increasing expression of C-Myc is associated with poor prognosis of patients diffuse large B-cell lymphoma. However, some studies have shown that high expression of C-Myc might be one of the mechanisms of disease progression and abrupt change, therefore, it can promote the transformation of chronic myeloid leukemia. Besides, the high expression of C-Myc may lead to the decrease of the sensitivity of chronic myelogenous leukemia cells to imatinib...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xiao-Wen Ma, Lin-Hua Yang
Chronic lymphocytic leukemia(CLL)is a malignant hematologic disease with heterogeneous clinical course, about two thirds of patients exhibit a long survival, but the remaining third of patients show aggressiveness of disease or poor response to conventional treatment even drug resistance. Studies suggested that tumor microenvironment possesses a critical effect in CLL disease progression in recent years. Monocyte/macrophage, as an important componens of tumor microenvironment, play a promotive role in the growth and drug reistance of tumor cells by direct cell-to-cell contact, secreting cytokines and suppressing antitumor responses...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Cheng-Ming Fei, Chun-Kang Chang
Bone marrow (BM) microenvironment appears to play an important role in the pathogenesis of hematological malignancies. Apart from soluble factors and direct cell-cell contact, the extracellular vesicles (EVs) were identified as a third mediator for cell communication within BM microenvironment. Recently, more and more evidences have demonstrated that EVs are also involved in the dysregulation of the BM microenvironment in patients with hematological malignancies. Therefore this review focuses on the biological characteristics of EVs, the clinical value of EVs as biomarkers, the BM microenvironment reprogramming in hematological malignancies by EVs, and the potential role of EVs in drug resistance and therapy of hematological malignancies...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Li Wang, De-Xun Ma, Shu-Feng Liu, Chun-Ji Gao
Graft-versus-host disease (GVHD) is a frequently encountered serious complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT), it limits the success and widespread use of allo-HSCT. Mesenchymal stem cells (MSCs) are selected as ideal prophylactic and treatment means for GVHD during allo-HSCT due to their unique immunomodulatory and regenerative properties. Herein, the recent research progress about the prevantive and therapeutic effects of MSCs on GVHD and several issues related with the applications of MSC, including whether MSCs increasing risk of primary disease relapse and infection, impact of several clinical parameters on the clinical response to MSCs, and the prevantive and therapeutic effect of MSC-derived extracellular vesicles on GVHD are systematically reviewed...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Zi-Xian Liu, Fang Dong, Sen Zhang, Ming-Zhe Han, Hideo Ema
Mitochondria are double-membrane organelles existing only in eukaryotic cells. Mitochondria perform various important functions,such as producing energy,regulating signal transduction,and contributing to stress response. Recent studies have highlighted an important role of mitochondria in the determination of hematopoietic stem cells (HSC) fate. Limited biogenesis or timely clearance of mitochondria is an important way against oxidative stress,which favors the quiescence of HSC. Accumulation of mitochondria may lead to proliferation of HSC,even the aging of HSC...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Bin Fan, Fen Huang, Yu-Hong Zhang, Gan-Hong Mei
OBJECTIVE: To evaluate the effects of peripheral introvascular central catheters (PICC) on the catheter-associated bloodstream infections (CRBSI) and the formations of catheter-related bloodstream infections. METHODS: Total of 483 patients with hematologic malignancies admitted in our hospital from January 2013 to December 2016 were enrolled in this study, and 612 PICC catheterization were conducted. The median time of catheterization was 101 d (48 ∼ 184 d) . The incidence rates of CRBSI and CRTC were observed, and the CRBSI and CRTC associated variables were also analyzed...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Min Xue, Qin-Qin Pan, Su Fan, Xiao-Yan Wang, Fang Zhao, Tai-Xiang Liu, Li-Li Shi, Ling Ma, Jie Sheng
OBJECTIVE: To estimate the size of HLA -Ⅰ class typed platelet apheresis donor bank. METHODS: A total of 16062 blood samples from Chinese Han voluntary unrelated marrow donors in Jiangsu were included in this study. Luminex-SSO was used to detect the HLA -Ⅰ class(A,B locus) antigens. The probability of finding at least one HLA matched unrelated donor was calculated based on the HLA -I class phenotype frequency. RESULTS: The population genetic data of HLA -Ⅰ class in Jiangsu were obtained, the optinal bans size in HLA typed apheresis plateler donor registry databane hrad been estimated by evaluating the population genetic data of HLA-1 class same donor...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jian-Feng Chen, Wen-Chao Li, Le Chang, Dan Cao, Jian-Hua Tang, Chen Xiao, Chuan-Xing Zhang
OBJECTIVE: To evaluate the performance of the chemiluminescence immune assay (CLIA) and the electro-chemiluminescence immuneoassay(ECLIA) for Treponemapallidum antibody(anti-TP) screening in blood donors. METHODS: The sero-panel samples from NCCL were tested with ELISA, CLIA and ECLIA assays synchronously to evaluate their performances respectively. RESULTS: The sensitivity and the negative predictive value of the CLIA were 100%, which were the same as one kind of ELISA, and better than the other ELISA; The specificity of the CLIA was 88...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Zheng-Min Liu, Rui Wang, Li-Qin Huang, Jing-Hui Hu, Le Chang, Wei Zhen, Peng Wang, Fang Wang, Chao Wei, Shao-Wen Zhu, Jin-Feng Zeng, Yan-Hua Shi, Wei Zheng, Lu-Nan Wang, Hong-Wei Ge
OBJECTIVE: To evaluate the necessity and suitability of the anti-HCV ELISA teot gray zone setted up by 7 blood station laboratories. METHODS: 7 blood station laboratories were coded as 1, 2, 3, 4, 5, 6 and 7 respectively; 8 kinds of ELISA reagents were coded as A, B, C, D, E, F, G and H respectively. 1 or 2 of 8 ELISA reagents produced by different manufactories were used to detect the anti-HCV in specimens of same group by 7 blood station laboratories; the Westen blot was used to detect the specimens with difference of detected results so as to difine the serological status of specimens...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yun-Jing Zheng, Qin Lu, Yan-Hua Yao, Hai-Long He, Jian-Qin Li, Pei-Fang Xiao, Shao-Yan Hu
OBJECTIVE: To investigate the gene mutation of patients with WAS gene defect and its correlation with clinical manifestations. METHODS: Thirty-one patients consulted in Children's Hospital of Soochow University from January 2013 to February 2018 were enrolled in this study. The hot pot mutations of WAS gene in 31 patients were detected and related clinical phenotypes were analyzed retrospectively. RESULTS: All patients were male. The median onset age was 1 month (range, 0-83 months)...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xiao-Yu Chen, Shun-Qing Wang
OBJECTIVE: To investigate the change of microbial diversity and its relation with gastrointestinal (GI) graft-versus-host disease (GVHD) following allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: Fecal samples were collected at the time point of 2 weeks before transplantation (pre-transplant group), 1 month after transplantation (post-tranplant group) and onset of GI GVHD in 65 hematonosis patients, which were also collected in 26 donors and 10 healthy subjects (control group)...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yi-Hui Li, Zhe Liu, Huan Li, Ying-Xi Xu, Hai-Yan Xing, Ke-Jing Tang, Zheng Tian, Min Wang, Qing Rao
OBJECTIVE: To explore the effect of damage of bone marrow stroma cells induced by chemotherapeutic drug on the function of normal hematopoitic cells. METHODS: Senescence cells were detected by flow cytometry after SA-β-gal staining; real-time PCR was used to detect the expression of a serial molecules in bone marrow stromal cell line OP9 cells; the expression of γ-H2AX was determined by flow cytometry after histone γ-H2AX staining; the colony forming ability of hematopoietic cells was tested by colony formation assay...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Dong-Ya Li, Jian-Hua Mao, Wei Zhang, Xin-Jie Chen, Bing Xiao, Zheng Ruan, Yun Wang, Guo-Xiong Jiang, Xiao-Feng Shi, Xiao-Dong Xi
OBJECTIVE: To establish 293T cell lines stably expressing Calpain-cleavage related α3 cytoplasmic tail mutants, and to explore the effect of amino acid motifs in integrin β3 cytoplasmic tail on αⅡbβ3-mediated cell function. METHODS: 293T cell lines stably co-expressing human wild type integrin αⅡb and full length β3 or mutant β3, including β3-ΔNITY (β3 cytoplasmic tail NITY motif deleted), β3-Δ754 (β3 cytoplasmic tail TNITYRGT motif deleted) and β3-Δ759 (β3 cytoplasmic tail RGT motif deleted) were established...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Li-Yan Guo, Pei-Long Lai, Su-Xia Geng, Xiao-Mei Chen, Yu-Lian Wang, Tian Huang, Xin DU, Jian-Yu Weng
OBJECTIVE: To investigate the effects of exosomes from human umbilical cord mesenchymal stem cells on the development of Treg and TH17 cells. METHODS: Exosomes from the serum-free-culture supernatants of hUC-MSC were harvested by ultracentrifugation. The electron microscopy, nanoparticle tracking analysis and western blot were used to identify the hUC-MSC-exosomes, such as the morphology, the paticle chameter, and the protein content. The PBMC stimulated with anti-CD3/CD28 were incubated with the exosomes for five days, and then the percentage changes of Treg and TH17 cells were analyzed by using flow cytometry...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ya-Ru Fu, Yang-Yang Sun, Hong-Xing Wang, Xiao-Dun Liu, Ting-Yu Qu
OBJECTIVE: To establish a novel method to isolate endothelial progenitor cells(EPC) from cryopreserved umbilical cord blood (cryoUCB), to investigate the biological characteristics of EPC and to improve the rate of EPC obtained from cryoUCB. METHODS: Twelve cryoUCB samples during 2000 to 2001 years were collected from allogeneic cord blood bank, cryoUCB was thawed rapidly in a water bath at 37 ℃, total nucleated cells (TNCs) were washed by phosphate-buffered saline (PBS)...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Bin Yan, Yang He, Shi-Qi Lu, Meng-Qiao Xu, Qi Wang, Yi-Ming Zhao, Chang-Geng Ruan
OBJECTIVE: To establish a novel flow cytometric immunobead array (FCIA) for detecting plasma von Willebrand factor activity (vWF:GPIbR) and apply it in ischemic stroke (IS). METHODS: Microspheres coated with anti-human platelet glycoprotein Ibα (GPIbα) monoclonal antibody SZ151 IgG, were incubated with recombinant fragment of GPIbα, then added ristocetin and plasma, finally incubated with FITC-conjugated sheep-anti-human vWF IgG polyclonal antibody, and detected by flow cytometry...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Dong Zhou, Li-Jun Mu, Qi Zhang, Dan Huang, Xue-Yu Chen
OBJECTIVE: To study the etiology of macrocytic anemia in elderly patients and to evaluate the diagnostic significance of laborotory tests. METHODS: 133 elderly macrocytic anemia patients, whose age>60 years old, hemoglobin<100 g/L, mean red cell volume(MCV)>100 fL, and bone marrow cell test was performed, and these patients were grouped according to diseases, and the bilirubin, lactate dehydrogenase, folic acid, vit B12 and serum ferritin were tested, then the results of tests were compared and analyzed...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ting-Xiao Li, Fu-Ting Sun, Bao-Jun Ji
OBJECTIVE: To investigate the correlation of IgG subclasses with blood cell parameters in the patients with autoimmune hemolytic anemia (AIHA). METHODS: Thirty-four patients with AIHA (except C3d types) of immune complex type (IgG+C3d) and single IgG type, including 26 cases of primary AIHA and 8 cases of secondary AIHA from December 2010 to August 2016 in our hospital were selected and enrolled in AIHA group; 30 healthy persons were selected and enrolled in healthy control group...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
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