Andrea Ossato, Carlo Gasperoni, Michela Stella, Giulia Montagner, Diletta Trojan, Giuseppe Giovagnoni, Michele Giannini, Francesca Realdon, Valeria Mezzadrelli, Nicola Realdon, Teresa Zuppini, Roberto Tessari
Musculoskeletal allografts represent an important practice in orthopedic surgeries and the demand for them has been growing. For this reason, in order to reduce clinical risk and to more efficiently manage the increase of allograft usage and also to optimize timing of the surgeries, the thawing and washing processes with aseptic technique were centralized in the department of Hospital Pharmacy. This study describe the design and execution of an adapted Media Fill Test (MFT) to demonstrate aseptic thawing and washing of allografts...
September 20, 2023: Cell and Tissue Banking
Saber Saharkhiz, Zohreh Abdolmaleki, Mohammad Amin Eslampour
Spermatogonia stem cells (SSCs) are a unique cell population maintaining male spermatogenesis during life, through their potential for proliferation and differentiation. The application of silicon nanoparticles (SNs) and hyaluronic acid (HA) to induce the differentiation of SSCs seems promising. Herein, we investigate the effect of SN and HA scaffolds on the progression of SSCs spermatogenesis in mice. Initially SSCs were isolated from healthy immature mice and cultured on prepared scaffolds (HA, SN, and HA/SN) in a 3D culture system...
September 7, 2023: Cell and Tissue Banking
Leila Taghizadeh Momen, Arash Abdolmaleki, Asadollah Asadi, Saber Zahri
Utilization of acellular scaffolds, extracellular matrix (ECM) without cell content, is growing in tissue engineering, due to their high biocompatibility, bioactivity ad mechanical support. Hence, the purpose of this research was to study the characteristics and biocompatibility of decellularized rat skin scaffolds using the osmotic shock method. First, the skin of male Wistar rats was harvested and cut into 1 × 1 cm2 pieces. Then, some of the harvested parts were subjected to the decellularization process by applying osmotic shock...
September 3, 2023: Cell and Tissue Banking
Elizaveta Padalko, Luc Colenbie, Alain Delforge, Nadine Ectors, Johan Guns, Romain Imbert, Hilde Jansens, Jean-Paul Pirnay, Marie-Pierre Rodenbach, Ivan Van Riet, Anne Vansteenbrugge, Gilbert Verbeken, Muriel Baltes, Hilde Beele
With the present paper, the Working Group on Cells, Tissues and Organs and other experts of the Superior Health Council of Belgium aimed to provide stakeholders in material of human origin with advice on critical aspects of serological and nucleic acid test (NAT) testing, to improve virological safety of cell- and tissue and organ donation. The current paper focusses on a number of preanalytical variables which can be critical for any medical biology examination: (1) sampling related variables (type of samples, collection of the samples, volume of the sample, choice of specific tubes, identification of tubes), (2) variables related to transport, storage and processing of blood samples (transport, centrifugation and haemolysis, storage before and after centrifugation, use of serum versus plasma), (3) variables related to dilution (haemodilution, pooling of samples), and (4) test dependent variables (available tests and validation)...
August 25, 2023: Cell and Tissue Banking
Noor Rahman, Rafiullah Khan, Tanvir Hussain, Nabi Ahmed
No abstract text is available yet for this article.
August 24, 2023: Cell and Tissue Banking
Zainab Kamal, Arundeep Kaur Lamba, Farrukh Faraz, Shruti Tandon, Archita Datta, Nasreen Ansari, Zaid Kamal Madni, Jaya Pandey
The presence of bone morphogenetic proteins in demineralized freeze-dried bone allograft (DFDBA) are responsible for developing hard tissues in intraosseous defects. The most common mode of sterilization of bone allografts, i.e., Gamma rays, have dramatic effects on the structural and biological properties of DFDBA, leading to loss of BMPs. Ultraviolet-C radiation is a newer approach to sterilize biodegradable scaffolds, which is simple to use and ensures efficient sterilization. However, UV-C radiation has not yet been effectively studied to sterilize bone allografts...
August 14, 2023: Cell and Tissue Banking
Noor Abdelwahab, Mohammadhamed Shahsavari, Kezhou Wu, Leila Laouar, Tamara D Skene-Arnold, Janet A W Elliott, Nadr M Jomha
Osteochondral allograft transplantation is a successfully proven method to repair articular cartilage defects and prevent the degenerative effects of osteoarthritis. The number of osteochondral transplantations that can be performed each year is limited by availability of donor cartilage tissue and storage time constraints. Osteochondral transplantation success has been linked to high chondrocyte viability of the donor cartilage tissue at the time of implantation. Determining optimal storage conditions for donor cartilage is essential for tissue banks to safely provide quality cartilage tissue...
August 8, 2023: Cell and Tissue Banking
S Amitha Banu, Abhijit M Pawde, Khan Sharun, E Kalaiselvan, Shivaraju Shivaramu, Karikalan Mathesh, Vikas Chandra, Rohit Kumar, Swapan Kumar Maiti, Med Ram Verma, Karam Pal Singh, Amarpal
Biomaterials capable of managing wounds should have essential features like providing a natural microenvironment for wound healing and as support material for stimulating tissue growth. Eggshell membrane (ESM) is a highly produced global waste due to increased egg consumption. The unique and fascinating properties of ESM allow their potential application in tissue regeneration. The wound healing capacity of bone marrow-derived mesenchymal stem cells (BM-MSCs), ESM, and their combination in rabbits with full-thickness skin defect (2 × 2 cm2 ) was evaluated...
August 4, 2023: Cell and Tissue Banking
Feng Zhuo, Xiaojing Jia, Zongru Wang, Yeyong Zhang, Xinfeng Yan
Knee osteoarthritis (KOA) is a chronic joint disease characterized by the degeneration of articular cartilage. In this study, we explored the potential therapeutic effects of platelet-rich plasma (PRP) and identified molecular targets for treating KOA. A rat model of KOA was established via the Hulth method and primary knee joint chondrocytes were isolated to evaluate the effects of PRP and shRNA targeting p65 (sh-p65). ELISA was used to detect inflammatory factors, including IL-6, IL-1β, and TNF-α...
July 27, 2023: Cell and Tissue Banking
Jeevitaa Kshersagar, Akshay A Kawale, Kishor Tardalkar, Mrunal N Damle, Leena R Chaudhari, Rakesh Sharma, Meghnad G Joshi
Platelet Rich Plasma (PRP) contains high concentrations of growth factors, therefore, PRP activation results in their release, stimulating the process of healing and regeneration. The study was conducted to check whether activated platelet-rich plasma (aPRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced disturbed endometrium. Seventy-two female Wistar rats were randomly assigned into the control group, disturbed endometrium (DE) group and aPRP treated group...
July 19, 2023: Cell and Tissue Banking
Liang Cao, Hui-Qian Zhang, Yu-Qi He, Ping-Jiang An, Lei-Luo Yang, Wei Tan, Gang Liu, Chun-Qing Wang, Xiao-Wei Dou, Qing Li
Cerebrospinal fluid-contacting neurons (CSF-cNs) act crucial role in chemosensory and mechanosensory function in spinal cord. Recently, CSF-cNs were found to be an immature neuron and may be involved in spinal cord injury recovery. But how to culture it and explore its function in vitro are not reported in previous research. Here, we first reported culture and identification of CSF-cNs in vitro. We first established a protocol for in vitro culture of CSF-cNs from the cervical spinal cord of mice within 24 h after birth...
June 27, 2023: Cell and Tissue Banking
Mehdi Rasouli, Roya Fattahi, Ghader Nuoroozi, Zeinab Zarei-Behjani, Maliheh Yaghoobi, Zeinab Hajmohammadi, Simzar Hosseinzadeh
Oxygen pressure plays an integral role in regulating various aspects of cellular biology. Cell metabolism, proliferation, morphology, senescence, metastasis, and angiogenesis are some instances that are affected by different tensions of oxygen. Hyperoxia or high oxygen concentration, enforces the production of reactive oxygen species (ROS) that disturbs physiological homeostasis, and consequently, in the absence of antioxidants, cells and tissues are directed to an undesired fate. On the other side, hypoxia or low oxygen concentration, impacts cell metabolism and fate strongly through inducing changes in the expression level of specific genes...
June 26, 2023: Cell and Tissue Banking
Yangyang Jin, Qi Sun, Rongxing Ma, Ruifeng Li, Ruiqi Qiao, Jikai Li, Limin Wang, Yongcheng Hu
Tendon injuries repair is a significant burden for orthopaedic surgeons. Finding a proper graft material to repair tendon is one of the main challenges in orthopaedics, for which the requirement of substitute for tendon repair would be different for each clinical application. Among biological scaffolds, the use of decellularized tendon increasingly represents an interesting approach to treat tendon injuries and several articles have investigated the approaches of tendon decellularization. To understand the outcomes of the the approaches of tendon decellularization on effect of tendon transplantation, a literature review was performed...
June 24, 2023: Cell and Tissue Banking
Vojtech Horvath, Alzbeta Svobodova, Joao Victor Cabral, Radovan Fiala, Jan Burkert, Petr Stadler, Jaroslav Lindner, Jan Bednar, Martina Zemlickova, Katerina Jirsova
This study aimed to evaluate the efficacy of cryopreserved amniotic membrane (AM) grafts in chronic wound healing, including the mean percentage of wound closure per one AM application, and to determine whether the healing efficiency differs between AM grafts obtained from different placentas. A retrospective study analyzing inter-placental differences in healing capacity and mean wound closure after the application of 96 AM grafts prepared from nine placentas. Only the placentas from which the AM grafts were applied to patients suffering from long-lasting non-healing wounds successfully healed by AM treatment were included...
May 25, 2023: Cell and Tissue Banking
Huifen Zang, Zhaohui Wang, Qingqing Wu, Lei Shi, Ge Chen
Mesenchymal stem cells (MSCs) derived extracellular vesicles, which have been shown to possess therapeutic effects for many diseases. However, how hypoxic conditions would affect exosomal microRNA expression in human umbilical cord MSCs (hUC-MSCs) is currently not investigated. This study aims to investigate the potential function of in vitro microRNAs of hUC-MSC cultured under normoxic and hypoxic conditions. Extracellular vesicles secreted from hUC-MSCs cultured in normoxic (21% O2 ) and hypoxic (5% O2 ) conditions were collected for microRNA identification...
May 24, 2023: Cell and Tissue Banking
Carlos Alexandre Curylofo Corsi, Claudia Tarcila Gomes Sares, Fabiola Mestriner, Jéssyca Michelon-Barbosa, Vinicius Flora Dugaich, Timna Varela Martins, Alex Martins Násare, Roberta Ribeiro Costa Rosales, Maria Cecília Jordani, José Carlos Alves-Filho, Rodolfo Borges Dos Reis, Mauricio Serra Ribeiro, Christiane Becari
Primary cell cultures are essential tools for elucidating the physiopathological mechanisms of the cardiovascular system. Therefore, a primary culture growth protocol of cardiovascular smooth muscle cells (VSMCs) obtained from human abdominal aortas was standardized. Ten abdominal aorta samples were obtained from patients diagnosed with brain death who were organ and tissue donors with family consent. After surgical ablation to capture the aorta, the aortic tissue was removed, immersed in a Custodiol® solution, and kept between 2 and 8 °C...
May 5, 2023: Cell and Tissue Banking
Marco Lux, Ralf Haller, Bettina Giere, Bianca Lindner, Michael Harder, Stefano Mastrobuoni, Ramadan Jashari
More than 1000 donated aortic and pulmonary valves from predominantly European tissue banks were centrally decellularized and delivered to hospitals in Europe and Japan. Here, we report on the processing and quality controls before, during and after the decellularization of these allografts. Our experiences show that all tissue establishments, which provide native cardiovascular allografts for decellularization, meet comparably high-quality standards, regardless of their national origin. A total of 84% of all received allografts could be released as cell-free allografts...
May 3, 2023: Cell and Tissue Banking
Jiamin Mao, Lexi Huang, Yiyang Ding, Xiaoyu Ma, Quanming Wang, Lei Ding
Background Collagenases are frequently used in chondrocyte isolation from articular cartilage. However, the sufficiency of this enzyme in establishing primary human chondrocyte culture remains unknown. Methods Cartilage slices shaved from femoral head or tibial plateau of patients receiving total joint replacement surgery (16 hips, 8 knees) were subjected to 0.02% collagenase IA digestion for 16 h with (N = 19) or without (N = 5) the pre-treatment of 0.4% pronase E for 1.5 h...
May 3, 2023: Cell and Tissue Banking
Shao-Lin Ji, Xiao-Dan Zhao, Li-Min Wang, Cheng-Gang Pang, Wen-Jing Li, Kun-Xiu Song, Rong-Xing Ma, Rui-Feng Li, Jing-Yu Zhang, Yong-Cheng Hu
Decalcified bone matrix (DBM) is a widely used alternative material for bone transplantation. In the DBM production process, an effective particle size and the highest utilization rate of raw materials can be achieved only through multiple high-speed circulating comminution. The rat posterolateral lumbar fusion model (PLF) is the most mature small animal model for the initial evaluation of the efficacy of graft materials for bone regeneration and spinal fusion. To evaluate the differences in the in vivo osteogenic effects of DBM pulverization through 1, 5, 9, and 14 high-speed cycles, sixty athymic rats were divided into six groups: single cycling crushing (CC1), 5 cycles of crushing (CC5), 9 cycles of crushing (CC9), 13 cycles of crushing (CC13), autogenous bone graft (ABG) and negative control (NC)...
May 3, 2023: Cell and Tissue Banking
Shima Norozi, Mrazieh Ghollasi, Ali Salimi, Raheleh Halabian, Mohsen Shahrousvad
Mesenchymal stem cells with tissue repair capacity involve in regenerative medicine. MSCs can promote bone repair when employed with nano scaffolds/particles. Here, the MTT and Acridine Orange assay enabled the cytotoxic concentration of Zinc oxide nanoparticles and Polyurethane evaluation. Following culturing adipose tissue-derived MSCs, ADSCs' proliferation, growth, and osteogenic differentiation in the presence of PU with and without ZnO NPs is tracked by a series of biological assays, including Alkaline Phosphatase activity, Calcium deposition, alizarin red staining, RT-PCR, scanning electron microscope, and immunohistochemistry...
April 27, 2023: Cell and Tissue Banking
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