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Journal of Immunoassay & Immunochemistry

B A Iwalokun, O Akinloye, B E Udoh, K O Akinyemi
Multidrug resistant (MDR) S. aureus infections continue to account for excess mortality in hospital and community settings and constitute a rising global health problem. However, data on the efficacy and mechanism of actions of alternative solutions like silver nanoparticles in developing countries are lacking. This study investigated anti-staphylococcal activity of silver nanoparticles (AgNP) against local strains from Nigeria. A total 119 clinical isolates of S. aureus from five Nigerian laboratories categorized as MRSA (n = 52) and MSSA (n = 67) by PCR were studied...
January 29, 2019: Journal of Immunoassay & Immunochemistry
Evren Doruk Engin
Traditionally, definitive diagnosis of infectious diseases is made by cultivation of the causative agent, while various antigens and antibodies as biomarkers of various diseases are detected by commercially available ELISA kits. PCR has emerged as a major innovation that greatly accelerated the accumulation of genomic and transcriptomic data, yet it has also revolutionized microbial diagnostics by enabling the detection of pathogen nucleic acid. Despite the advantages of and vast experience in ELISA and PCR, the next generation research and diagnostic tools have to fulfill the requirements of systems and synthetic biology era...
January 20, 2019: Journal of Immunoassay & Immunochemistry
Еlena S Don, Sergey А Bobrovnik, Gordon Sherriff, Andrey А Myslivets, Sergey А Tarasov, Оleg I Epstein
Selection of a suitable assay to measure the activity of drugs based on released-active forms (RA forms of Abs) is an important step during their investigation. In this study, ELISA was utilized to examine the effect of RA forms of Abs to interferon gamma on the interaction between monoclonal anti-interferon gamma antibodies and human interferon gamma. The data showed that such RA forms of Abs are able to modulate the antibody interaction with interferon gamma, and it was suggested that the observed influence of RA forms of Abs on 'antibody-antigen' interaction could be used to analyze the activity of this kind of drugs...
January 20, 2019: Journal of Immunoassay & Immunochemistry
Cristiana Tanase, Radu Albulescu, Monica Neagu
No abstract text is available yet for this article.
January 11, 2019: Journal of Immunoassay & Immunochemistry
Maria-Linda Popa, Radu Albulescu, Monica Neagu, Mihail Eugen Hinescu, Cristiana Tanase
Building the future of precision medicine is the main focus in cancer domain. Clinical trials are moving toward an array of studies that are more adapted to precision medicine. In this domain, there is an enhanced need for biomarkers, monitoring devices, and data-analysis methods. Omics profiling using whole genome, epigenome, transcriptome, proteome, and metabolome can offer detailed information of the human body in an integrative manner. Omes profiles reflect more accurately real-time physiological status...
January 11, 2019: Journal of Immunoassay & Immunochemistry
Haseeb Ahsan
Many ingredients in cosmetic products help to develop complex formulations that improve the quality of life in terms of disease prevention, health maintenance, beauty enhancement, and building self-esteem. The beneficial effects promoted from the use of biomolecule-rich substances into the formulations of various topical application products are considered useful ingredients in cosmetic and therapeutic applications. This review article attempts to understand the various biomolecules found in cosmetic products, particularly macromolecules, which may have an important role in prevention or preservation...
December 26, 2018: Journal of Immunoassay & Immunochemistry
Stephen Oluwasegun Adetunji, Emmanuel Donbraye, Lateefah Alawode-Obabiyi, Oluwasola Akinniyi
Hepatitis B virus (HBV) infection has been rated 10th among the killer diseases. Occult HBV infection goes undetected with the widely used HBsAg screening in most hospital thereby encouraging progression into complications such as liver cirrhosis and hepatocellular carcinoma. This work therefore aimed at evaluating HBV serological profiles among the population of apparently healthy hospital attendees and their proportion of susceptibility to HBV infection. 239 participants were recruited for this study. Their blood samples were screened for the HBV markers - HBsAg, anti-HBs, HBeAg, anti-HBe, and anti-HBc...
December 20, 2018: Journal of Immunoassay & Immunochemistry
Alina Fudulu, Adrian Albulescu, Gabriela Anton
Cervical cancer, the fourth leading cause of cancer-associated deaths among women worldwide, is associated with human papilloma virus (HPV) infection. Despite the prophylactic HPV vaccination and the implementation of cervical and HPV-based screening programs, a significant increase in cervical cancer incidence is estimated by the year 2020. Thus, further development of diagnostic tools that allow detection and risk assesment in genital HPV infection is necessary. A special interest is focused on the HPV viral proteins whose expression might be of use either as primary screening tool or in conjunction with other markers (cellular proteins, HPV DNA, PAP test)...
December 5, 2018: Journal of Immunoassay & Immunochemistry
Bogdan Ionel Vatu, Stefan-Alexandru Artene, Adeline-Georgiana Staicu, Adina Turcu-Stiolica, Catalin Folcuti, Alexandra Dragoi, Catalina Cioc, Stefania-Carina Baloi, Ligia Gabriela Tataranu, Cristian Silosi, Anica Dricu
In recent years, immunotherapy has raised the interest of many studies and provided different perspectives for the therapeutic management of high grade glioma. Our meta-analysis focused on the effectiveness of dendritic cell (DC) therapy and viral therapy (VT) in clinical trials. Fourteen eligible studies have been evaluated and the results suggest the improvement of both OS (HR = 0.65) (p < 0.0001) and PFS (HR = 0.59) (p = 0.01) for patients receiving DC therapy. The data for VT showed a slight improvement in terms of OS (HR = 0...
November 30, 2018: Journal of Immunoassay & Immunochemistry
Carlos A Duarte, Sheila Chávez, Yordanka Masforrol, Samy Puertas, Taimí Paneque, Anna C Ramirez, Dionne Casillas, Pedro Puente, Hilda Garay, Celia Fernández-Ortega
The synthetic peptide CIGB-210 is a promising anti-HIV drug candidate shown to inhibit HIV replication in MT4 cells at the nanomolar range by triggering the rearrangement of vimentin intermediate filaments. Sensitive and specific analytical methods are required for pharmacological studies of CIBG-210 in animals. In this study, we describe the development of a competitive ELISA for the quantitative determination of CIGB-210 using an anti-CIGB-210 hyperimmune serum. After optimization of all the steps, the assay exhibited a dynamic range from 11...
November 29, 2018: Journal of Immunoassay & Immunochemistry
Haseeb Ahsan
The oral mucosa protects the host against invading antigens and pathogenic microorganisms and contains an elaborate immune system and remains in a relative state of health despite the heavy antigen load. The oral barrier is exposed to unique and diverse communities of commensal microbial communities that are known to play immune-stimulatory roles in oral inflammatory diseases. Saliva is secreted from the salivary glands and has multiple functions, including mouth cleaning and protection, antibacterial effects, and digestion...
November 29, 2018: Journal of Immunoassay & Immunochemistry
M Manasa, P Revathi, M Prudhvi Chand, V Maroudam, P Navaneetha, G Dhinakar Raj, P B Kavi Kishor, B De, P Rathnagiri
This study was conducted to develop and evaluate protein-G-based lateral flow assay (LFA) for rapid serodiagnosis of brucellosis in various domesticated animal species. The assay diagnostic performance was tested with 144 reference and 356 field sera samples and then compared with other serological assays. Results revealed that LFA showed 89% and 99% sensitivity and specificity, respectively, when compared with competitive ELISA as the gold standard. This study demonstrated LFA alone as a potential serodiagnostic assay for rapid serodiagnosis of brucellosis in various domesticated animal species...
November 26, 2018: Journal of Immunoassay & Immunochemistry
Abdolali Moshfe, Bahador Sarkari, Nasir Arefkhah, Reza Nikbakht, Reza Shahriarirad, Zahra Rezaei, Ali Jamshidi, Farid Moradian
The current study aimed at the seroepidemiological survey of human cystic echinococcosis (CE) in nomadic people in Boyer-Ahmad District in the southwest of Iran. One thousand and five nomads were selected by cross-sectional sample collection in nomadic tribes of Boyer-Ahmad District in the southwest of Iran. Blood samples were taken from each individual, and the sera were tested for detection of anti-hydatid cyst antibodies using antigen B-ELISA. A predesigned questionnaire which contained basic epidemiological and individual information related to hydatid cyst was filled for each subject during sample collecting...
November 21, 2018: Journal of Immunoassay & Immunochemistry
Amr M Mohamed, Ahmed M Ashshi, Ghada A Abou El-Ella, Mohamed A Basalamah, Maher N Alandiyjany, Aiman A Alsaegh
The current study proposed that previously characterized individual antigenic proteins could represent potential replacement for conventional purified protein derivative (PPD) in tuberculosis skin testing when used in cocktails triggered by suitable TLR-stimulants that would provide the missing pro-inflammatory stimulus. Three different cocktails of previously selected antigens, including C1 (ESAT-6/CPF-10/MPB-83); C2 (ESAT-6/MPB-64/MPB-83); and C3 (CPF-10/MPB-64/MPB-83), were evaluated in vitro using lymphocytic proliferation and IFN-γ production assays, as well as mRNA and protein expression levels of TNF-α, IL-12p40, and IL-2 as pro-inflammatory molecules...
November 19, 2018: Journal of Immunoassay & Immunochemistry
Nazila Ariaee, Abdolreza Varasteh, Farahzad Jabbari Azad, Mojtaba Sankian
Atopic diseases have an increasing trend worldwide during the last two decades. Determining the main cause of allergic diseases, allergens, is the first step in managing and improving the issue, usually is done by Skin Prick tests (SPTs). Having allergenic extract in high quality is desired to perform a reliable SPT. Several parameters of extracts are considered including composition, stability, potency, preservation conditions, and unit definition. In this review, these factors have been explained pointing to factors might have profitable points or harmful drawback in the quality of allergen extracts...
November 13, 2018: Journal of Immunoassay & Immunochemistry
Liliana-Roxana Balahura, Raluca-Ioana Stefan-Van Staden, Jacobus Frederick Van Staden, Hassan Y Aboul-Enein
Immunoassay technique performs a fast, simple, reliable, and sensitive analysis of different compounds, being applied in several areas of interest such as clinical analysis for medical diagnosis, as well as in environmental analysis, and food quality control. The latest research activities in this field are represented by the attempts to achieve a low limit of detection by developing of new signal amplification strategies, eliminate the interferences, and decrease the cost of analysis.
November 7, 2018: Journal of Immunoassay & Immunochemistry
Narges Farshidi, Maliheh Moghaddam, Vahid Yaghoubi, Seyed Hasan Ayati, Abdol-Reza Varasteh, Mojtaba Sankian
The incidence of grape (Vitis vinifera) allergy in the northeast of Iran is second to melon allergy. Type IV chitinase is one of the major grape allergens. The current study investigates the level of type IV chitinase in four grape variants for the first time in Khorasan Razavi Province using a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA). This assay was developed using a polyclonal antibody as a capture antibody and monoclonal antibody as a secondary one. Finally, the amount of type IV chitinase was measured by the validated ELISA test...
October 22, 2018: Journal of Immunoassay & Immunochemistry
Haseeb Ahsan
Arginine is derived from dietary intake, body protein breakdown, or endogenous de novo arginine production. Arginine methylation of non-histone proteins is used in transcriptional regulation. Protein-arginine methylation is used for regulation of transcriptional and various physiological pathological processes. Protein methylation may affect protein-protein, protein-DNA, or protein-RNA interaction. Arginine has an effect on the DNA-binding activity of NF-κB, a dominant transcriptional factor in inflammation...
2018: Journal of Immunoassay & Immunochemistry
Gnanadurai John Fletcher, Raghavendran Anantharam, Kalaivani Radhakrishnan, Unnati Singh, Amaldev Karunakaran, Visalakshi Jeyaseelan, Priya Abraham
Hepatitis B virus-e-antigen (HBeAg) is a viral marker to assess hepatitis B virus (HBV) replication. We have evaluated the reliability of three commonly available HBeAg immunoassays using World Health Organization-International Standard and clinical samples. In addition the performance of enzyme immunoassays (EIAs) was assessed by kinetic binding and reagent exchange experiments. Analytical and diagnostic sensitivity were significantly different among HBeAg assays (P < 0.01). The affinity of capture/detector antibodies varied significantly between EIAs (P < 0...
2018: Journal of Immunoassay & Immunochemistry
A V Opayele, G N Odaibo, O D Olaleye
Livestock handlers are occupationally exposed to Rift Valley fever virus (RVFV) because of frequent and longer contact with mosquito vectors, animal blood and other tissues. We determined the seroprevalence of RVF virus infection among livestock handlers in Ibadan, Nigeria. Blood samples were collected from 265 workers in two major livestock markets and a livestock farm. Questionnaires were administered to obtain information on risk factors that may be associated with RVF virus transmission. The blood samples were tested using enzyme-linked immunosorbent assays (ELISA) to detect multispecies anti-RVF virus IgG/IgM and human anti-RVF virus IgM...
2018: Journal of Immunoassay & Immunochemistry
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