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Bioelectrochemistry

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https://read.qxmd.com/read/30771661/electron-transfer-interpretation-of-the-biofilm-coated-anode-of-a-microbial-fuel-cell-and-the-cathode-modification-effects-on-its-power
#1
Yamin Yang, Chansoo Choi, Guorong Xie, Jong-Deok Park, Shao Ke, Jong-Sung Yu, Juanjuan Zhou, Bongsu Lim
Biofilm-coated electrodes and outer cell membrane-mimicked electrodes were examined to verify an extracellular electron transfer mechanism using Marcus theory for a donor-acceptor electron transfer. Redox couple-bound membrane electrodes were prepared by impregnating redox coenzymes into Nafion films on carbon cloth electrodes. The electron transfer was believed to occur sequentially from acetate to nicotinamide adenine dinucleotide (NAD), c-type cytochrome, flavin mononucleotide (FMN) (or riboflavin (RBF)) and the anode substrate...
February 8, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30769178/influence-of-the-electrode-material-on-ros-generation-and-electroporation-efficiency-in-low-and-high-frequency-nanosecond-pulse-range
#2
Paulius Ruzgys, Vitalij Novickij, Jurij Novickij, Saulius Šatkauskas
Electroporation is a widely-used methodology for permeabilization of cells using pulsed electric field (PEF). In this paper, we compare the electroporation efficiency in terms of molecular transport and the generated reactive oxygen species (ROS) between low (1 Hz) and high (1 MHz) frequency nanosecond range PEF bursts. We used aluminum, copper and stainless-steel electrodes and evaluated the influence of electrode material on ROS generation and electroporation. Bursts of 25 or 50 pulses of 7-14 kV/cm amplitude and 200 ns duration were applied, and the results were compared to those obtained using electroporation with pulses of equivalent energy in conventional microsecond range...
February 6, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30745281/biosensor-for-direct-bioelectrocatalysis-detection-of-nitric-oxide-using-nitric-oxide-reductase-incorporated-in-carboxylated-single-walled-carbon-nanotubes-lipidic-3-bilayer-nanocomposite
#3
Filipa O Gomes, Luísa B Maia, Joana A Loureiro, Maria Carmo Pereira, Cristina Delerue-Matos, Isabel Moura, José J G Moura, Simone Morais
An enzymatic biosensor based on nitric oxide reductase (NOR; purified from Marinobacter hydrocarbonoclasticus) was developed for nitric oxide (NO) detection. The biosensor was prepared by deposition onto a pyrolytic graphite electrode (PGE) of a nanocomposite constituted by carboxylated single-walled carbon nanotubes (SWCNTs), a lipidic bilayer [1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-di-(9Z-octadecenoyl)-3-trimethylammonium-propane (DOTAP), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol (DSPE-PEG)] and NOR...
February 5, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30690422/electrochemical-characterization-of-lhcii-on-graphite-electrodes-potential-dependent-photoactivation-and-arrangement-of-complexes
#4
Paulina Piotrowska, Magdalena Łazicka, Adriana Palińska-Saadi, Bohdan Paterczyk, Łucja Kowalewska, Joanna Grzyb, Magdalena Maj-Żurawska, Maciej Garstka
Light-dependent electrochemical properties of the light harvesting complexes of Photosystem II (LHCII) and the corresponding interactions with screen-printed graphite electrodes (GEs) are determined. No exogenous soluble redox mediators are used. LHCII isolated from spinach leaves are immobilized on GE by physical adsorption and through interactions with glutaraldehyde. Importantly, the insertion of LHCII into the pores of a GE is achieved by subjecting the electrode to specific potentials. Both trimeric and aggregated forms of LHCII located within the graphite layer retain their native structures...
January 22, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30690423/effect-of-alternating-current-and-bacillus-cereus-on-the-stress-corrosion-behavior-and-mechanism-of-x80-steel-in-a-beijing-soil-solution
#5
Hongxia Wan, Dongdong Song, Cuiwei Du, Zhiyong Liu, Xiaogang Li
The effects of alternating current (AC) and Bacillus cereus (B. cereus) on the stress corrosion behavior and mechanism of X80 pipeline steel were investigated in a Beijing soil solution in this study. Both AC and B. cereus can promote the generation of pits in X80 steel. B. cereus is a type of nitrate-reducing bacteria and hence nitrate reduction occurs at the bottom of the corrosion product film or the biofilm. Meanwhile, the anode accelerates the dissolution of X80 steel and generates some pits in the local working area...
January 21, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30703737/fabrication-of-an-imprinted-electrochemical-sensor-from-l-tyrosine-3-methyl-4-nitrophenol-and-gold-nanoparticles-for-quinine-determination
#6
F Azadmehr, K Zarei
This study employed a molecularly imprinted composite (MIC) to develop a selective and very sensitive sensor for the determination of quinine. To fabricate the MIC sensor, 3-methyl-4-nitrophenol (MNP) and L-tyrosine (Tyr) were simultaneously electrodeposited in acidic media containing HAuCl4 to entrap Au nanoparticles (AuNPs) into the formed composite network. The effect of Tyr on the sensitivity and selectivity of the sensor and its electrochemical performance were evaluated. The signal reduction of the Fe2+ probe during differential pulse voltammetry (DPV) was used to determine the concentration of quinine...
January 18, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30735920/scalability-of-self-stratifying-microbial-fuel-cell-towards-height-miniaturisation
#7
Xavier Alexis Walter, Carlo Santoro, John Greenman, Ioannis A Ieropoulos
The scalability of bioelectrochemical systems is a key parameter for their practical implementation in the real-world. Up until now, only urine-fed self-stratifying microbial fuel cells (SSM-MFCs) have been shown to be scalable in width and length with limited power density losses. For practical reasons, the present work focuses on the scalability of SSM-MFCs in the one dimension that has not yet been investigated, namely height. Three different height conditions were considered (1 cm, 2 cm and 3 cm tall electrodes)...
January 9, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30654242/a-two-phase-gradual-silver-release-mechanism-from-a-nanostructured-tialv-surface-as-a-possible-antibacterial-modification-in-implants
#8
Eva Pruchova, Michaela Kosova, Jaroslav Fojt, Petra Jarolimova, Eva Jablonska, Vojtech Hybasek, Ludek Joska
Titanium biomaterials are widely used in the medical field due to their biocompatibility and excellent corrosion and mechanical resistance. However, these materials have no antibacterial properties. To obtain an antibacterial active surface, a nanostructure of Ti6Al4V alloy was created. This specific nanostructure contained nanotubes and micro-cavities and was used as a substrate for silver anchoring. The electrochemical approach to silver reduction was studied. It is a common approach for silver deposition and in this work, inhomogeneities in the nanostructure were used as a preferential area for silver localisation...
January 8, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30641310/electrochemical-evidence-for-direct-interspecies-electron-transfer-between-geobacter-sulfurreducens-and-prosthecochloris-aestuarii
#9
Lingyan Huang, Xing Liu, Jiahuang Tang, Linpeng Yu, Shungui Zhou
The syntrophic photosynthesis via direct interspecies electron transfer (DIET) between Geobacter sulfurreducens and Prosthecochloris aestuarii has opened a new paradigm of microbial phototrophy. However, it is still unknown whether this photosynthetic DIET can be mediated by an electrical conductor. Here we report first the photosynthetic DIET in a two-chamber microbial fuel cell (photo-MFC). The photo-MFC worked well and generated a maximum current of 0.6 mA/m2 , which validated photosynthetic DIET via the titanium wire...
January 5, 2019: Bioelectrochemistry
https://read.qxmd.com/read/30623791/electrochemical-spectroscopic-and-molecular-docking-studies-on-the-interaction-of-calcium-channel-blockers-with-dsdna
#10
Suniya Shahzad, Burcu Dogan-Topal, Leyla Karadurmus, Mehmet Gokhan Caglayan, Tuğba Taskin Tok, Bengi Uslu, Afzal Shah, Sibel A Ozkan
This study presents evaluation of the possible interaction mechanism between calf thymus dsDNA and three calcium antagonists; nifedipine, lercanidipine and amlodipine. The interactions between Nifedipine-dsDNA and Lercanidipine-dsDNA were investigated by differential pulse voltammetry using two different interaction methods; at the dsDNA-electrochemical biosensor surface and in bulk incubated solution. Amlodipine was used as model drug in bulk incubated solution. The decrease in the peak current of guanine and adenine were used as an indicator for confirmation of the interaction event in acetate buffer of pH 4...
December 28, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30614442/microbial-phyto-power-systems-a-sustainable-integration-of-phytoremediation-and-microbial-fuel-cells
#11
REVIEW
Beenish Saba, Mohsin Khan, Ann D Christy, Birthe Veno Kjellerup
Constructed wetland microbial fuel cells (CW-MFCs) or phyto-power systems are integrated bioelectrochemical systems (BES) that can sustainably harvest electricity from the anaerobic respiration of rhizospheric bacteria. This integration of techniques shows a promise in phytoremediation of wastewater along with bioenergy generation. In CW-MFCs, electrons harvested in anaerobic respiration of bacteria proliferating in the rhizospheric zone are electrochemically coupled with electron acceptors at the aerobic cathode submersed in water...
December 28, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30597452/a-multi-functional-minimally-disruptive-portable-electrochemical-system-based-on-yeast-co-3-o-4-au-spes-for-blood-lead-ii-measurement
#12
Jing Nie, Bin He, Yu-Jiao Zang, Wei Yin, Liang-Ri Han, Wen-Fei Li, Chang-Jun Hou, Dan-Qun Huo, Mei Yang, Huan-Bao Fa
A minimally-disruptive portable electrochemical system is constructed by combining a hand-held syringe as reservoir with disposable screen-printed electrodes (SPEs) modified with a simple and efficient yeast/Co3 O4 /Au material for lead determination by a square-wave voltammetry (SWV) method. Not only can it preserve the operation and advantages of the conventional electrochemical procedure, but it also integrates sampling, filtering and analysis to make the determination of lead convenient and effective at higher and lower concentration levels...
December 21, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30590224/%C3%AE-lactoglobulin-as-a-potential-carrier-for-bioactive-molecules
#13
S Świątek, P Komorek, G Turner, B Jachimska
In this study, the interaction and binding behavior of anesthetic tetracaine (TET) with bovine β-lactoglobulin (LGB) isoform A and a mixture of isoforms A and B were investigated under varying environmental conditions (pH, ionic strength, concentration, LGB-TET complex molar ratio). A wide range of analytical techniques (dynamic light scattering (DLS), electrophoretic mobility, UV-Vis spectroscopy, circular dichroism (CD), quartz crystal microbalance (QCM-D) were used to analyze the physicochemical properties of the complexes in bulk solution and on the surface of gold...
December 20, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30597451/regenerated-silk-fibroin-membranes-as-separators-for-transparent-microbial-fuel-cells
#14
Grzegorz Pasternak, Yuejiao Yang, Bruno Bosquiroli Santos, Federico Brunello, Martin M Hanczyc, Antonella Motta
In recent years novel applications of bioelectrochemical systems are exemplified by phototrophic biocathodes, biocompatible enzymatic fuel cells and biodegradable microbial fuel cells (MFCs). Herein, transparent silk fibroin membranes (SFM) with various fibroin content (2%, 4% and 8%) were synthesised and employed as separators in MFCs and compared with standard cation exchange membranes (CEM) as a control. The highest real-time power performance of thin-film SFM was reached by 2%-SFM separators: 25.7 ± 7...
December 17, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30590223/interfacial-electron-transfer-between-geobacter-sulfurreducens-and-gold-electrodes-via-carboxylate-alkanethiol-linkers-effects-of-the-linker-length
#15
M Füeg, Z Borjas, M Estevez-Canales, A Esteve-Núñez, I V Pobelov, P Broekmann, A Kuzume
Geobacter sulfurreducens (Gs) attachment and biofilm formation on self-assembled monolayers (SAMs) of carboxyl-terminated alkanethiol linkers with varied chain length on gold (Au) was investigated by electrochemical and microscopic methods to elucidate the effect of the surface modification on the current production efficiency of Gs cells and biofilms. At the initial stage of the cell attachment, the electrochemical activity of Gs cells at a submonolayer coverage on the SAM-Au surface was independent of the linker length...
December 12, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30563748/cytochrome-p450-genes-play-central-roles-in-transcriptional-response-by-keratinocytes-to-a-high-voltage-alternating-current-electric-field
#16
Masayo Aoki, Noriko M Matsumoto, Yuri Okubo, Rei Ogawa
The endogenous electric field (EF) of skin wounds plays an important role in the biological processes that underlie wound healing. Treatments that modulate wound-EFs promote healing. However, the mechanism(s) that underlie this effect remain unclear. Agilent-based microarrays were used to determine the transcriptomes of the keratinocyte line HaCaT, normal human dermal fibroblasts, and the human dermal endothelial cell line HMEC-1 before and after high-voltage alternating current (AC)-EF (14,000 V, 90 Hz) treatment...
December 11, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30563747/influence-of-the-current-density-in-moderate-pulsed-electric-fields-on-p-putida-f1-eradication
#17
Efrat Emanuel, Pogreb Roman, Rivka Cahan
Eradication of P. putida F1 was investigated as a function of current density in pulsed electric fields of 6.7, 4, 2.8, 2 and 1 kV/cm. The pulse numbers were 200, 2000, 5000 and 10,000 and were performed by a series of trains of 500 pulses (except for the 200 pulses). The frequency was 100 Hz and pulse durations were 10 μs or 20 μs as indicated for each experiment. The current density range was 0.02 ± 0.01 to 5.2 ± 0.5 Acm-2 . A clear tendency for increasing bacterial death was found as a result of increasing the current density in each of the tested electric field strengths...
December 11, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30579249/corrosion-behavior-of-x80-pipeline-steel-in-the-presence-of-brevibacterium-halotolerans-in-beijing-soil
#18
Zhong Li, Hongxia Wan, Dongdong Song, Xiaodong Liu, Ziyu Li, Cuiwei Du
In this study, the corrosion behavior of X80 pipeline steel in the presence of Brevibacterium halotolerans (B. halotolerans) was investigated by surface analysis and electrochemical measurements. Results show that B. halotolerans can attach to the surface of X80 steel, and the entire surface is covered with B. halotolerans. The corrosion products induced by B. halotolerans were FeOOH, Fe2 O3 and FeSO4 . X80 steel corrosion was accelerated in the presence of B. halotolerans and was susceptible to pitting corrosion...
December 5, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30554019/electrochemical-behavior-of-titanium-and-platinum-in-dicarboxilic-amino-acids-solution
#19
E A Kornyushova, A V Kashevskii, K Y Arsent'ev, B G Pushkarev, S B Nikiforov, A Y Safronov
Titanium and platinum samples as components of bimetallic implants for the osteoregeneration process have been modified in solutions modeling biological systems and studied by means of cyclic voltammetry, electrochemical impedance spectroscopy and scanning probe microscopy. While aspartic and glutamic acids did not adsorb significantly on platinum in the potential region investigated, the presence of the amino acids affects oxide layer growth on the titanium surface under anodic polarization. The two studied amino acids behave differently on the titanium electrode surface due to differences in adsorption modes of these substances...
December 4, 2018: Bioelectrochemistry
https://read.qxmd.com/read/30540973/a-comparison-of-microbial-fuel-cell-and-microbial-electrolysis-cell-biosensors-for-real-time-environmental-monitoring
#20
Ademola Adekunle, Vijaya Raghavan, Boris Tartakovsky
This study compares the biosensing performance of a microbial fuel cell (MFC) and a microbial electrolysis cell (MEC). Initial tests provided a qualitative comparison of MFC and MEC currents after the anode compartment liquid (anolyte) was spiked with acetate, or sulphates of NH4 + , Na+ , Mg2+ , Fe2+ , or a fertilizer solution. Current measurements showed that the MFC sensor had a faster response time, higher sensitivity, and faster recovery time after the spike. Following the spike tests, the MFC and MEC were operated in a continuous flow mode at several influent concentrations of acetate, and sulphates of NH4 + , Na+ , and Fe2+ ...
December 1, 2018: Bioelectrochemistry
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